Académique Documents
Professionnel Documents
Culture Documents
Section: 112
121
181
241
308
428
542
A508
(AT)
0.02
0.07
0.255
0.309
0.324
0.552
A450
1.06
0.495
0.764
0.792
0.699
0.78
AINFA508
AINFAT/AINFAO=[MCL]/[MCL]O
LN(AIN
F)
0.961
0.911
0.726
0.672
0.657
0.429
1.186
1.124
0.896
0.829
0.811
0.530
0.171
0.117
-0.110
-0.187
-0.210
-0.636
AINF..*[MLN[M 1/
CLO]
-CL]T [M=CL
(GIVES
]T
CONCENTR
ATION AT
TIME T)
0.020 -3.915
50.143
0.019 -3.968
52.895
0.015 -4.195
66.372
0.014 -4.273
71.706
0.014 -4.295
73.343
0.009 -4.721
112.31
8
CHM151Y
Name: Ruo yi (Caroline) LinStudent #: 1001333112
778
915
0.479
0.655
1200
0.673
0.755
Section: 112
0.502
0.326
0.620
0.402
-0.479
-0.910
0.010
0.007
-4.564
-4.996
0.584
0.397
0.490
-0.713
0.008
-4.799
1495
0.564
0.417
0.515
-0.664
0.009
-4.750
1802
0.602
0.379
0.468
-0.760
0.008
-4.845
2400
0.61
0.371
0.458
-0.781
0.008
-4.867
95.986
147.80
1
121.37
1
115.55
0
127.13
4
129.87
6
Table 1. Absorbances given over time and associated calculations. Ainf is the
absorbance of the sample as time approaches infinity. The value of A 450 used in the
calculation of Ainf .is the average of the measured A450, omitting the first two
samples.
500
1000
1500
2000
2500
3000
-1.000
-2.000
ln[M-Cl] (M)
-3.000
-4.000
-5.000
f(x) = - 0x - 4.17
R = 0.6
-6.000
time (s)
Graph 1. Plot of time against ln[M-Cl]. If the reaction is first-order, this plot should
be a line of the form ln[M-Cl]=-kt+ln[M-Cl]o. The calculated value of [M-Cl]o from this
graph is 0.0154M. k is 0.0004.
CHM151Y
Name: Ruo yi (Caroline) LinStudent #: 1001333112
Section: 112
120.0
100.0
1/[M-Cl] (M-1)
80.0
60.0
40.0
20.0
0.0
500
1000
1500
2000
2500
3000
Time (s)
Graph 2. Plot of time against 1/[M-Cl]. If the reaction is second-order, this plot
should be a line of the form 1/[M-Cl]=kt+1/[M-Cl] o. The calculated values of [M-Cl]o
and k would be 0.0151M and 0.0355 respectively.
1/T
0.03
0.03
0.03
0.02
0.02
0.02
T (oC)
30
35
40
45
50
60
k (run
0.0000 0.0001 0.0003 0.0005 0.0004 0.0039
1)
730
55
05
91
00
30
k (run
0.0000 0.0001 0.0003 0.0005
0.0038
2)
728
45
01
98
70
k (run
0.0000 0.0001 0.0003 0.0005
0.0040
3)
741
51
03
87
20
lnk (1)
-9.53
-8.77
-8.10
-7.43
-7.82
-5.54
lnk (2)
-9.53
-8.84
-8.11
-7.42
-5.55
lnk (3)
-9.51
-8.80
-8.10
-7.44
-5.52
lnk
-9.52
-8.80
-8.10
-7.43
-7.82
-5.54
(avg)
Table 2. Rate constants at different temperatures from the lab manual. The value
of k found from graph 1 was placed in the 50 degrees Celsius spot in run 1.
CHM151Y
Name: Ruo yi (Caroline) LinStudent #: 1001333112
Section: 112
lnk
0.00
0.02
0.02
-1.00
ln(average k)
-2.00
-3.00
-4.00
-5.00
-6.00
f(x) = - 237.03x - 1.92
-7.00
f(x) = - 209.35x - 2.78
0.96
Linear (Avg R
(no=
R
=outlier))
0.86
-8.00
-9.00
-10.00
0.03
0.03
0.04
Avg (no outlier)
Graph 3. A plot of (1/T) against lnk. According to the Arrhenius equation, the such a
plot should yield a line of the form lnk=(-E a/R)(1/T)+lnA, where A is the Arrhenius
constant, R the gas constant, and Ea the activation energy. Two trendlines were
plotted on this graph: one for all points, and another for all points excluding the
outlier at T=50degrees C. The values of k were taken by averaging the 3 values of k
at each temperature given in the lab manual.
Sample Calculations for table 1.
[M-Cl]o=0.240g(1mol/285.49g)(0.050L)=0.0168mol/L
A450=AVERAGE(0.764, 0.792, 0.699, 0.78, 0.673, 0.755)=0.744
Ainf=(1.005/0.762)A450=0.981
Ao=(0.175/0.762)A450=0.171
For the first row (time=121s):
Ainf-A508=0.981-0.02=0.961
(Ainf-At)/(Ainf-Ao)=(0.981-0.02)/(0.981-0.171)=0.961/0.810=1.186 (This value is equal
to [M-Cll]t/[M-Cl]o by equation (14) of the lab manual.)
ln((Ainf-At)/(Ainf-Ao))=ln(1.186)=0.171
[M-Cl]t=[M-Cl]o((Ainf-At)/(Ainf-Ao))=0.0168(1.186)=0.020 (notice that this is a
nonsensical result which indicates that the concentration of [M-Cl] increased in the
first two minutes of the hydration reaction; see discussion for details.)
ln[M-Cl]t=ln(0.020)=-3.915
1/[M-Cl]t=1/0.020=50.1
All other such calculations were done using excel.
Discussion
Conclusions
CHM151Y
Name: Ruo yi (Caroline) LinStudent #: 1001333112
Section: 112
The Lambert-Beer law in conjunction with a simple spectrometer can be used to find
the concentration of an unknown sample of a given chemical compound. For this
experiment, and unknown concentration of Copper Sulphate which gave a voltage
of 3.60V was calculated to have a concentration of 2.61x10 -3M. Using coloured
filters, the validity of the Lambert Beer law in an experimental setting was
confirmed. Limitations of the Lambert-Beer law were observed using the
phenomenon of chlorophyll photofluorescence. In conclusion, the Lambert-Beer law
is shown to be a useful tool for the identifications of the concentrations of
compounds, although not without limitations.
References
1. Hollas, Michael J. Modern Spectroscopy. John Wiley & Sons, 1996.
2. Sawyer, Heineman, and Beebe. Chemistry Experiments for Instrumental
Methods, John Wiley & Sons, 1984.
3. Skoog, Holler and Nieman. Principles of Instumental Analysis, 5th ed.,
Saunders College Publishing, 1998.