CURRICULUM VITAE

NAME

V.Venkata Suryanarayana

DATE AND PLACE OF BIRTH

31st August 1975

Visakhapatnam, Andhra pradesh
India

NATIONALITY

Indian

SEX

Male

MARITAL STATUS

Single

PERMANENT ADDRESS

39-23-28/1, Vamsi nagar

Madhavadhara, Visakhapatnam
Andhrapradesh 530007
India
Ph: 00-91-9440481554

ADDRESS FOR CORRESPONDENCE Plot No-64, H.No- 1-19-164/5,

Venkata Sai Nagar Colony

West Venkatapuram
Trimulghery post
Secunderabad-500015
India
Email: suryasan@yahoo.com,
Mobile: +91-9492925781

ACADEMIC QUALIFICATIONS
Degree

Year

Ph.D

Subjects studied

University/Colle

Division/Perce

ge
Centre

ntage

2001-

for

2008

Cellular

and

Molecular
Biology,
M.Tech

1997-99

(Biotechnology)

Biochemical Engg

Hyderabad
Anna University, First/ 9.05

Bioprocess Modelling Chennai

(CGPA out of

& simulation

10)

Enzyme Engineering
Rec. DNA Tech
Bioinformatics
Biochemistry
Immunology
M.Sc

1995-97

(Biochemistry)

Bioorganic Chemistry Andhra

Metabolism

University,

Enzymology

Visakha Patnam

First/ 70

Molecular Biology
Genetics
Clinical Biochemistry
Immunology
B.Sc

1992-95

RESEARCH EXPERIENCE

Cell Biology
Mathematics

Andhra

Biochemistry

University,

Chemistry

Visakha Patnam

First/ 75

1.

Ph.D. CCMB, India. Doctoral thesis Molecular status of Detoxification genes

and functional proteomics in recurrent early pregnancy loss (REPL)
Supervisor: Dr. Lalji Singh, Director, Centre For Cellular and Molecular
Biology, Hyderabad.
Description: REPL is a heterogeneous condition experienced by about 20%
couples and in half of the cases the reasons are unknown. Present study is aimed at
dissecting out the molecular genetic markers responsible for REPL in South Indian
women. Mutational screening of genes involved in detoxification, estrogen
metabolism, blood homeostasis, vascular activity etc provided us with exciting
results with a strong association between the risk of recurrent miscarriages and the
polymorphic markers studied was found. In addition, a number of novel
polymorphisms that were not reported earlier had been discovered. This data is
valuable for appropriate model development to assess the risk associated with a
particular or combination of alleles.
Another part of the study is targeted at the analysis of blood platelet proteome and
a comparison between normal and REPL females to fish out the candidate proteins
that can be used as diagnostic markers. Platelet proteome showed consistent
differences between cases & controls and the identification of the proteins by
MALDI-TOF and peptide mass fingerprinting was done.
Techniques Used: DNA isolation from blood, PCR primer design, synthesis and
amplification of DNA, RFLP, DNA Sequencing using ABI-3730 automated
sequencer, Sequence analysis using Gene tools, Mutant phenotype prediction using
Transcription factor binding site search, phylogenetic footprinting analysis,
comparative modelling. Statistical analyses include Significance tests, Correlation
and regression, power calculations. Protein analytical techniques included Blood
cell separation in a dextran gradient, platelet protein preparation, quantitation,
Isoelectric Focussing in pre-cast as well as manually developed pH gradient gels,
SDS-PAGE, staining to visualize the protein spots, image analysis using PDquest
software from BIORAD, preparation of peptides from gel spots for MALDI-TOF

MS analysis, analysis of MALDI spectra, database searching to find out the

peptide mass fingerprints.
2.

M.Tech Dissertation: carried out for a period of six months at Centre for
Biotechnology, Anna University, Chennai
Title:Multivariable optimization of poly--hydroxy butyrate production by
Azotobacter beizerinckii by the use of response surface methods.
Supervisor: Prof. Kuntala jayaraman and Dr. R.Sachidanandham
Description: Azotobacter beijerinckii is known to grow and produce the
biodegradable plastic, poly--hydroxy butyrate (PHB) using defined medium. In
the present study, we developed and optimised a process for the production of PHB
from molasses and Corn steep liquor (CSL) as the carbon and nitrogen sources.
The growth profile of A.beijerinckii was monitored and the product harvesting time
was optimised. Experiments were designed using the full factorial design and the
results were analysed by the 2D and 3D response surfaces. A mathematical model
was developed in terms of total Carbohydrate and Nitrogen content to predict the
yield of PHB. This was used to calculate the optimum molasses and CSL
concentrations that maximize the yield.
Techniques Used: Basic microbiological methods such as culture maintenance,
revival, media preparation, sterilisation, staining, microscopic examination;
Bioreactor operation (1lit, 2lit), shake flask batch experiments, PHB extraction
using a solvent extraction method, using STATGRAPHICS plus for WINDOWS
for experimental design, regression analysis, Design Expert software for studying
the response surfaces.

WORK EXPERIENCE

As a Scientist at Dr.Surapanenis Genomic Solutions Pvt Ltd since September 2007 till
date. Job responsibilities include gene isolation, cloning, and expression of protein in
prokaryotic system, protein purification and modification (fluorescent tagging, enzyme

coupling).
TEACHING EXPERIENCE

An experience of two years in Biotechnology and Biochemistry for postgraduate and under
graduate students at V.S.Lakshmi Womens College, Kakinada, an affiliated centre to Andhra
University. Subjects taught were Intermediary Metabolism, Enzymology, Clinical
Biochemistry, Molecular Biology, Industrial biotechnology, Immunology.

RESEARCH EXPERTISE

Biochemistry: Protein purification utilising the affinity chromatography technique, protein

estimations, Western Blotting, ELISA, Polyacrylamide Gel Electrophoresis, 2-D gel
electrophoresis, image analysis using soft wares like PDQUEST, Proteomic analysis using
techniques like MALDI-TOF. Qualitative and quantitative analysis of various classes of bio
molecules, enzyme assays and kinetic studies. Evaluation of clinical parameters with special
emphasis on biological fluids.
Molecular biology: Isolation of DNA and RNA from cells and tissues, PCR, RT-PCR,
agarose and polyacrylamide gel electrophoresis, DNA sequencing and Gene scan.
Microbiology: Culturing of bacterial cells, Staining, basic Bioreactor operations (up to 20L
level). Downstream operations involved in product purification.
Computational Skills: Working knowledge of computational tools such as SPSS, Epistat,
Statgraphics, MATLAB, Arlequin, GOLD, Swiss PDB modeller, Clustal, Sequence
assembling using Auto assembler, Gene tool. Programming knowledge in computer
languages such as C and PERL with orientation towards biological applications.

PUBLICATIONS

1. Venkata V Suryanaryana , Lakshmi Rao , Murthy K Kanakavalli , Venkata V

Padmalatha , Mamata Deenadayal , Lalji Singh. Role of CYP17 and CYP19

polymorphisms in idiopathic recurrent miscarriages among South Indian women.

Reprod Biomed Online. 2007 Mar ;14 (3):341-7.
2. Venkata Suryanarayana, Lakshmi Rao, Murthy Kanakavalli, Venkata Padmalatha,
Mamata Deenadayal, Lalji Singh . Recurrent early pregnancy loss and endothelial
nitric oxide synthase gene polymorphisms. Arch Gynecol Obstet. 2005 Dec 16: 1-6.
3. K. Anil kumar, K. Lakshmi Rao, Suryanarayana V Vedula, M.K. Kanakavalli,
Padmalatha V Vaddamani, Mamata Deenadayal, Nalini Gupta, B.N.Chakravarthy,
Lalji Singh (2005). Screening of the galactose-1-phosphate uridyltransferase gene in
Indian women with ovarian failure. Reproductive BioMedicine Online, Vol.11 (4),
444448.
4. Venkata Suryanarayana, Mamata Deenadayal, Lalji Singh (2004). Association of
CYP1A1 gene polymorphism with recurrent pregnancy loss in the South Indian
population, Human Reproduction, vol. 19 (11), 2648-2652.
5. K Arvind Babu, K Lakshmi Rao, M K Kanakavalli, V V Suryanarayana, A P Singh,
M Deenadayal and Lalji Singh (2004). CYP1A1, GSTM1 and GSTT1 genetic
polymorphism is associated with susceptibility to polycystic ovaries in South Indian
women. Reproductive BioMedicine Online, Vol.9 (2), 194200
6. M.Purushothaman, R.K.I.Anderson, S.Narayana, V.K.Jayaraman. (2001) Industrial
byproducts as cheaper medium components influencing the production of
polyhydroxyalkanoates (PHA)- biodegradable plastics. Bioprocess and Biosystems
Engineering. 24 (3), 131-136.
ACHIEVEMENTS

1.

Qualified the National Eligibility Test (NET), held by the Council for Scientific and
Industrial Research (CSIR), India in 2000.

2.

Awarded Junior Research Fellowships and Senior Research Fellowships by CSIR,

India

3.

Selected for Jawaharlal Nehru University fellowship to carry out M.Tech at Anna
University, Chennai in 1997.

4.

Secured 5th rank in the entrance test for admission into M.Sc Biochemistry in the year
1995.

5.

Won a best poster award for Detoxification gene polymorphisms- an etiological factor in
idiopathic early pregnancy loss (REPL) at 9th ADNAT & 30th ISHG symposium on
Molecular Medicine and Health at CCMB, Hyd, INDIA in the year 21-24 Feb 2005.

CONFERENCES AND SYMPOSIA ATTENDED

International Silver Jubilee Symposium (Nov2002) Current Excitement in Biology

organized by CCMB, Hyderabad, INDIA

Participated in 7th ADNAT (Association for the Promotion of DNA Fingerprinting and
other Technologies), a two day symposium on Proteomics & Micro array at CCMB,
Hyderabad, INDIA.

Presented a poster entitled Human Reproductive disorders; Understanding the

Genetic basis at C.C.M.B for the opening day in the year Nov 2003.

Participated & presented a poster in CME programme on Current Concepts & Research in
Infertility Obstetrics & Gynaecology in Jan 2004 at Owaisi Hospital & Research centre,
Hyderabad, INDIA & presented a poster entitled Chromosome defects & Human
health.

Participated in 8th ADNAT, a two day symposium on Comparative and Functional

Genomics at CCMB, Hyderabad, INDIA in the year 23-24 Feb 2004.

NIRRHISSRF (National Institute for Research in Reproductive Health Indian Society

for the Study of Reproduction and Fertility) Symposium on Advances and Challenges in
Reproductive Health Research in the Post Genomic Era, January 912, 2005, NIRRH,
Mumbai, India

Participated in 9th ADNAT & 30th ISHG (Indian Society of Human Genetics), a four
days symposium on Molecular Medicine and Health at CCMB, Hyderabad, INDIA in

the year 21-24 Feb 2005.

Participated in 31st annual ISHG conference, a three day symposium held at Jawaharlal
Nehru University, New Delhi, INDIA during 27Feb-1Mar 2006.

REFERENCES

Dr. Lalji Singh

Director,
Centre for Cellular and Molecular Biology,
Tarnaka, Habsiguda, Hyderabad, A.P.INDIA
Email: lalji@ccmb.res.in
Ph.No: +91-40-27160789
Fax No: +91- 40-27160311
Dr.K.Thangaraj,
Scientist, W 410,
Centre for Cellular and Molecular Biology,
Tarnaka, Habsiguda, Hyderabad, A.P.INDIA
Email: thangs@ccmb.res.in
Ph.No: +91-040-27192636
Fax:

+91-40-27160591

Ms.K.Lakshmi Rao,
Scientist, E 409,
Centre for Cellular and Molecular Biology,
Tarnaka, Habsiguda, Hyderabad, A.P.INDIA
Email: lakshmi@ccmb.res.in
Ph.No: +91-040-27192607
Fax :

+91-40-27160591

I assure that the information that has been given above is true and correct to my
knowledge.
V.V.Suryanarayana