Exercise 2: Skeletal Muscle Physiology: Activity 3: The Effect of Stimulus Frequency on Skeletal Muscle Contraction Lab

Report
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
1. During a single twitch of a skeletal muscle
You correctly answered: b. maximal force is never achieved.
2. When a skeletal muscle is repetitively stimulated, twitches can overlap each other and result in a stronger muscle
contraction than a stand-alone twitch. This phenomenon is known as
You correctly answered: c. wave summation.
3. Wave summation is achieved by
You correctly answered: a. increasing the stimulus frequency (the rate of stimulus delivery to the muscle).
4. Wave summation increases the force produced in the muscle. Another way to increase the force produced by a muscle
is to
You correctly answered: d. increase the number of activated motor units.

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Experiment Results
Predict Question:
Predict Question 1: As the stimulus frequency increases, what will happen to the muscle force generated with each
successive stimulus? Will there be a limit to this response?
Your answer : b. As the stimulus frequency increases, the muscle force generated by each successive stimulus will
increase. There will be a limit to this increase.
Predict Question 2: In order to produce sustained muscle contractions with an active force value of 5.2 grams, do you think
you will need to increase the stimulus voltage?
Your answer : a. yes
Stop & Think Questions:
Was there any change in the force generated by the muscle during the second stimulated twitch?
You correctly answered: c. Yes, the second twitch generated more muscle force.
Is the total muscle force generated by the higher frequency stimulation greater than the force generated in previous
stimulations?
You correctly answered: a. Yes, it is greater than the previous stimulations.
Does the force generated by the muscle change with each additional stimulus?
Your answer : a. As the stimulus frequency increased, the muscle tension generated by each successive stimulus also
increased and there was no limit to this increase.
Correct answer: b. As the stimulus frequency increased, the muscle tension generated by each successive stimulus also
increased, and a limiting maximum value was observed.
Experiment Data:
Voltage
8.5
8.5
8.5
8.5
10
8.5

Length
75
75
75
75
75
75

Stimulus
Single
Multiple
Multiple
Multiple
Multiple
Multiple

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Active Force
1.83
2.41
2.50
4.45
4.03
5.65

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Passive Force
0.00
0.00
0.00
0.00
0.00
0.00

Total Force
1.83
2.41
2.50
4.45
4.03
5.65

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Post-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.
1. Which of the following is not one of the ways that the body can increase the force produced by a skeletal muscle?
You correctly answered: d. application of higher voltages to the whole muscle
2. When a muscle receives a stimulus frequency that causes non-overlapping twitches to follow each other closely in time
such that the peak tension of each twitch rises in a stepwise fashion up to a plateau value, the result is known as
You correctly answered: c. treppe.
3. In this experiment the isolated skeletal muscle was repetitively stimulated such that individual twitches overlapped with
each other and resulted in a stronger muscle contraction than a standalone twitch. This phenomenon is known as
You correctly answered: c. wave summation.
4. Wave summation is achieved by
You correctly answered: a. increasing the rate of stimulus delivery (frequency) to the muscle.

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Review Sheet Results

1. What is the difference between stimulus intensity and stimulus frequency?
Your answer:
Intensidad, consiste en la carga de estmulo, en este caso elctrico, y medido en voltaje
Frecuencia, consiste en el numero de estimulos o las veces en que estimulamos al musculo.
2. In this experiment you observed the effect of stimulating the isolated skeletal muscle multiple times in a short period with
complete relaxation between the stimuli. Describe the force of contraction with each subsequent stimulus. Are these results
called treppe or wave summation?
Your answer:
Estos resultados se refieren al efecto escalera, en el que hay un tiempo de relajacion entre cada estimulo y su consiguiente
contraccin.
3. How did the frequency of stimulation affect the amount of force generated by the isolated skeletal muscle when the
frequency of stimulation was increased such that the muscle twitches did not fully relax between subsequent stimuli? Are
these results called treppe or wave summation? How well did the results compare with your prediction?
Your answer:
este resultado correspone a la sumacin.Este fenmeno ocurre cuando las ?bras musculares estn desarrollando tensin
y son estimuladas de nuevo antes de que se hayan relajado. Por eso la sumacin se consigue aumentando la
frecuencia de estimulacin
, o la rapidez con que se aplican los estmulos al msculo. Sucede porque las fibras musculares estn parcialmente
contradas.
4. To achieve an active force of 5.2 g, did you have to increase the stimulus voltage above 8.5 volts? If not, how did you
achieve an active force of 5.2 g? How well did the results compare with your prediction?
Your answer:
Se puede llegar aumentando la frecuencia de estmulos
5. Compare and contrast frequency-dependent wave summation with motor unit recruitment (previously observed by
increasing the stimulus voltage). How are they similar? How was each achieved in the experiment? Explain how each is
achieved in vivo.
Your answer:
Al aumentar el voltaje, las unidades motoras estimuladas son las mismas, en una sumacin va aumentando el numero de
unidades motoras activadas. Son similares, porque ambas llegan a aumentar la fuerza activa.

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