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Waste Management
journal homepage: www.elsevier.com/locate/wasman
Institute of Technology of Agricultural Products, National Agricultural Research Foundation, 1, Sof. Venizelou St., Lycovrissi, 14123 Athens, Greece
Technological Educational Institution of Kalamata, Department of Food Technology, Antikalamos, 24100 Kalamata, Greece
c
National Technical University of Athens, Department of Chemical Engineering, 9, Heroon Polytechniou St., Zographou 157 70, Greece
b
a r t i c l e
i n f o
Article history:
Received 9 July 2009
Accepted 7 April 2010
Available online 14 May 2010
a b s t r a c t
Considerable concern is caused by the problem of potato peel waste (PPW) to potato industries in Europe.
An integrated, environmentally-friendly solution is yet to be found and is currently undergoing investigation. Potato peel is a zero value waste produced by potato processing plants. However, bio-ethanol produced from potato wastes has a large potential market. If Federal Government regulations are adopted in
light of the Kyoto agreement, the mandatory blending of bio-ethanol with traditional gasoline in amounts
up to 10% will result in a demand for large quantities of bio-ethanol. PPW contain sufcient quantities of
starch, cellulose, hemicellulose and fermentable sugars to warrant use as an ethanol feedstock. In the
present study, a number of batches of PPW were hydrolyzed with various enzymes and/or acid, and fermented by Saccharomyces cerevisae var. bayanus to determine fermentability and ethanol production.
Enzymatic hydrolysis with a combination of three enzymes, released 18.5 g L 1 reducing sugar and produced 7.6 g L 1 of ethanol after fermentation. The results demonstrate that PPW, a by-product of the
potato industry features a high potential for ethanol production.
2010 Elsevier Ltd. All rights reserved.
1. Introduction
With the inevitable depletion of the worlds energy supply,
there has been an increasing worldwide interest in alternative energy sources (Lin and Tanaka, 2006). In recent years, increasing research and development efforts have been directed towards the
commercial production of ethanol as the most promising biofuel
from renewable resources. In many European countries the use
of bio-ethanol as an alternative fuel or gasoline supplement in
amounts up to 15% is highly recommended (Mojovic et al.,
2006). If Federal Government regulations are adopted based on
the Kyoto agreement, the mandatory blending of bio-ethanol with
traditional gasoline in amounts up to 10% will result in a demand
for large quantities of bio-ethanol. Many countries have implemented, or are in the process of implementing, programs providing for the addition of ethanol to gasoline. Fuel ethanol production
has increased remarkably due to the global demand to reduce oil
importation, thereby contributing towards boosting rural economies and improving air quality. The world ethyl alcohol production has reached approximately 51,000 million liters, whereas
the USA and Brazil are the main producers. On average, 73% of
1899
Fig. 1. Diagram of the potato crisps manufacturing and production of liquid wastes.
1900
Table 1
Chemical composition of potato peel waste (PPW).
Parameters
Moisture %
Total carbohydrate
Total soluble sugar
Reducing sugar
Starch
Nitrogen
Protein (Ntot 6.25a)
Fat
Ash
85.06
68.7
1
0.61
52.14
1.3
8
2.6
6.34
1901
Table 2
Fermentable reducing sugars released after enzymatic hydrolysis of PPW with various
enzyme combinations, reducing sugar (g L 1) at the end of fermentation and
fermentable sugar during fermentation.
Enzyme combinations
a
b
c
d
e
f
g
Table 3
Ethanol production (g L
fermentation.
Hydrolysate
reducing
sugar
Reducing
sugar after
fermentation
Consumed
sugar
14.06 0.67a
10.27 0.98b
16.82 1.01c
14.68 1.20a
16.99 1.17c
1.46 0.12
1.09 0.22
1.82 0.19
1.61 0.31
2.03 0.53
12.60 0.33
9.18 0.17
15.00 1.14
13.07 0.99
14.96 1.25
a
b
c
d
e
18.32 1.23d
2.11 0.28
16.21 1.54
18.48 0.65d
1.93 0.31
16.55 0.85
L: Liquozyme.
T: Ternamyl.
V: Viscozyme.
C: Celluclast.
a,b,c,d
Different superscripts are statistically signicant, p = 0.05.
Enzyme combinations
Ethanol (g L
Y (p/s)
% Max theoretical
yield
5.86 0.15a
4.19 0.10b
7.00 0.06c
6.05 0.23a
6.89 0.47c
0.462
0.459
0.467
0.460
0.456
92.40
91.80
93.38
92.10
91.24
7.58 0.24c
0.463
92.54
7.50 0.28c
0.458
91.64
L: Liquozyme.
T: Ternamyl.
V: Viscozyme.
C: Celluclast.
a,b,c,
Different superscripts are statistically signicant, p = 0.05.
1902
study. Moreover, the availability of manufacturers to become involved in this project and to devote resources to its implementation will provide a signicant contribution.
Acknowledgements
The authors wish to thank Mrs. Malli Basiliki and Mrs.
Chaidemenaki Katerina for their valuable help in the analytical
work.
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