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ANTHROPOLOGY
ABSTRACT: This study provides evidence of craniofacial growth variation between the sexes in juveniles of European descent. Data were collected from lateral cephalometric radiographs belonging to the Michigan Craniofacial Growth Study. The collection consists of longitudinal lateral
radiographs that represent individuals 516 years of age. Each radiograph was manually traced on hyprint vellum from which eight craniometric
points were identified. From these points, 20 craniofacial measurements were recorded and then analyzed by means of a canonical discriminant function analysis. Sex classification equations were then created by applying a backward stepwise procedure to the discriminant functions. The analysis
demonstrates the presence of sexually dimorphic differences in craniofacial growth. The neurocranium is the most sexually dimorphic region of the
juvenile craniofacial skeleton, until the onset of puberty. Size is the main source of variation with males having taller and longer heads than females.
Overall, sex classification in the sample ranges from 78 to 89% accuracy.
KEYWORDS: forensic science, forensic anthropology, sexual dimorphism, sex determination, craniofacial growth, skull identification
Two thousand four hundred and eighty-seven juveniles between
the ages of 5 and 16 were homicide victims in the United States from
2004 to 2007. Most juvenile homicides were committed against
males, the majority of which occurred between the ages of 1316
(14). The trends in juvenile victimization coupled with the fact that
many juvenile skeletal remains are recovered in archaeological settings, make the development of forensic anthropological techniques
for juvenile skeletal identification a necessity. There are numerous
identification issues in the archaeological and forensic analysis of
juvenile skeletons; sex determination is the most important because
sex is not as easily identifiable in juveniles as it is in adults.
Determination of sex is a vital aspect of skeletal analysis and the
key for establishing skeletal profiles of unidentified individuals
(5,6). Previous studies have attempted to develop sex identification
standards for juvenile skeletal material, and a large body of work
has accumulated concerning morphological differences that center
on those regions that are most sexually dimorphic in adults, the
skull, and pelvic girdle (724). Unfortunately, many of these
attempts to develop sex identification standards for juvenile skeletal
remains have provided variable degrees of accuracy and according
to Komar and Buikstra (25), have failed to find convincing evidence of sexual dimorphism in preadolescent skeletons (p. 126).
1
Department of Anthropology, St. Lawrence University, 23 Romoda
Drive, Canton, NY 13617.
2
Current address: Department of Physical Therapy, Clarkson University,
Clarkson Hall, 59 Main St., Potsdam, NY 13699.
*Presented in part at the 76th Annual Meeting of the American Association of Physical Anthropologists, March 2831, 2007, in Philadelphia, PA,
and at the 60th Annual Meeting of the American Academy of Forensic Sciences, February 1823, 2008, in Washington DC.
Partially funded by the St. Lawrence University Jeffrey Campbell Graduate Fellowship.
Received 24 June 2010; and in revised form 1 Nov. 2010; accepted 27
Nov. 2010.
24
25
Females
Males
Total
50
50
50
50
50
49
50
50
50
50
50
49
100
100
100
100
100
98
26
Name
GOL
NOL
NBL
BOL
OBL
BBL
BPL
BNL
BSL
NSL
NPL
PBL
PPL
PNL
SPL
PSL
SGL
SOL
OPL
PBR
GlabellaOpisthocranion
NasionOpisthocranion
NasionBregma
BregmaOpisthocranion
OpisthocranionBasion
BasionBregma
BasionProsthion
BasionNasion
BasionSella
NasionSella
NasionProsthion
PNSBasion
PNSProsthion
PNSNasion
SellaPNS
ProsthionSella
SellaGlabella
SellaOpisthocranion
OpisthocranionProsthion
ProsthionBregma
variables that contributed the least to sex identification were eliminated from the analysis. Thus, the equations rely on the variables
that are most statistically associated with sex. The average of the
function centroids, within each discriminant formula, created sectioning points between males and females. Each classification
model was then cross-validated by using the leave-one-out function
of the canonical discriminant function analysis procedure.
Results
Three of the 20 functions produced by the canonical discriminant
function analysis were the most statistically significant and account
for 87.3% of the total variation (Table 3). Results from each of
these canonical discriminant functions represent metric differences
in craniofacial form between the sexes throughout development.
The first canonical discriminant function (CAN1) accounts for
71.9% of the total variance (Table 4) and displays high loadings
for PNSnasion length (PNL), PNSprosthion length (PPL),
nasionprosthion length (NPL), prosthionsella length (PSL), and
basionnasion length (BNL). The pattern of variation in this axis
represents important developmental changes in the craniofacial
skeleton as it grows over time (Fig. 2). A combination of facial
and neurocranial variables are most significant in this axis. CAN1
illustrates sequences of developmental differences between the
sexes in the facial skeleton and that an interaction with neurocranial
growth determines the growth trajectory of the face. Both sexes
show a similar developmental transition from childhood to puberty.
This transition occurs at the 1112 age-group when there is a trend
toward greater facial development. However, there are periods in
which facial size increase is slow, and there are periods in which
facial size increase is faster.
The position of the age-groups 56 and 1112 in CAN1 suggests
slow or decreased facial changes throughout development in these
two age-groups. Male and female differences at these two agegroups are only slightly evident. For the other age-groups, size
tends to change exponentially and as these facial size changes
occur, so does sexual dimorphism. In the earlier age-groups, female
facial growth is much faster and, consequently, females appear
much larger than males. At the transition into puberty, female facial
growth decreases and male facial growth increases. Therefore,
males appear much larger than females. The ages 56 and 1112
represent a reduction of craniofacial growth.
The second canonical discriminant function (CAN2), which
accounts for 10.6% of the total variance (Table 4), displays high
loadings for sellaglabella length (SGL), PNSprosthion length
(PPL), bregmaopisthocranion length (BOL), nasionprosthion
length (NPL), and prosthionsella length (PSL). The pattern of variation in this axis represents an illustration of craniofacial developmental growth velocity curves between the sexes (Fig. 3). Both
sexes follow a similar pattern of growth velocity in which there are
increases and decreases in the rate of craniofacial growth. A developmental transition from childhood to puberty is also evident at the
1112 age-groups. At this point in development, both sexes go
from a slowdown of craniofacial development, to having a slight
increase in craniofacial growth during puberty. CAN2 clearly illustrates that the majority of craniofacial growth is completed around
the age of six. The slight increases at the onset of puberty reflect
the development of secondary sexual characteristics.
In the third canonical discriminant function (CAN3), which
accounts for 4.8% of the total variance (Table 4), high loadings are
displayed for basionbregma length (BBL), nasionbregma length
(NBL), sellaglabella length (SGL), bregmaopisthocranion length
(BOL), and glabellaopisthocranion length (GOL). The pattern of
27
Wilks Lambda
Eigenvalue
% Variance
Cumulative %
Canonical Correlation
p > 0.05
0.030
0.182
0.319
5.059
0.746
0.335
71.9
10.6
4.8
71.9
82.5
87.3
0.914
0.654
0.501
0.000
0.000
0.000
CAN1
CAN2
CAN3
PNL
PPL
NPL
PSL
BNL
PBR
BPL
BSL
SPL
NSL
BBL
NBL
SGL
BOL
GOL
NOL
OPL
OBL
PBL
SOL
0.766*
0.687*
0.676*
0.654*
0.624*
0.576
0.522
0.521
0.462
0.452
0.368
0.287
0.452
0.212
0.368
0.383
0.412
0.043
0.167
0.166
)0.275
0.044*
)0.018*
)0.067*
)0.245
)0.100
)0.101
)0.329
)0.295
)0.120
)0.227
)0.147
0.139*
)0.005*
)0.080
)0.142
)0.127
)0.131
)0.127
)0.104
0.097
)0.198
0.026
0.070
0.340
0.389
0.038
0.225
0.180
0.399
0.715*
0.526*
0.511*
0.464*
0.462*
0.411
0.206
0.213
0.244
0.235
FIG. 2Sequence of developmental differences between the sexes in the facial skeleton. Solid bars represent males and clear bars represent females. The
horizontal axis represents the age-groups in the analysis and the vertical axis represents the discriminant score means of the first canonical discriminant function for each age-group. In the vertical axis, deviations from 0 represent the degree of change in craniofacial growth. This bar graph illustrates the significance of the first canonical discriminant function in which variables influencing facial growth are most meaningful. Both sexes show a similar developmental
transition from childhood to puberty. There are periods in which facial growth is slow and periods in which facial growth is faster. The 56 and 1112
age-groups show the slowest facial growth changes and represent the developmental periods in which males and females are most similar. From ages 56 to
ages 910 female facial growth is much faster and, consequently, females appear much lager than males. From ages 1112 to ages 1516 female facial
growth decreases and male facial growth increases. This represents the development of secondary sexual characteristics in males.
28
FIG. 3Craniofacial growth velocity differences between the sexes. Solid bars represent males and clear bars represent females. The horizontal axis represents the age-groups in the analysis and the vertical axis represents the discriminant score means of the second canonical discriminant function for each
group. In the vertical axis, deviations from 0 represent the degree of change in craniofacial growth. This bar graph illustrates the significance of the second canonical discriminant function. Neurocranial and facial variables exhibit a similar pattern of change. In this function, both sexes follow a similar pattern of craniofacial growth velocity with periodic changes in the rate of craniofacial growth. This function clearly illustrates that the majority of craniofacial
growth is completed around the age of six. The slight increases at the onset of puberty reflect the development of secondary sexual characteristics.
FIG. 4Sex differences in the juvenile craniofacial skeleton. Solid bars represent males and clear bars represent females. The horizontal axis represents
the age-groups in the analysis and the vertical axis represents the discriminant score means of the third canonical discriminant function for each group. In
the vertical axis, deviations from 0 represent the degree of change in craniofacial growth. This bar graph illustrates the significance of the third canonical
discriminant function. Most of the variation in this function derives from neurocranial variables, which represent clear-cut difference between the sexes. This
function demonstrates that sex differences in the craniofacial skeleton are established early in life. Further development of secondary sexual characteristics is
the result of enhancements of already established craniofacial features that are unique to males and females.
29
FIG. 5Developmental trajectories between the sexes. Females: circles with solid lines, Males: triangles with dotted lines. This two-dimensional plot represents the first and second canonical discriminant functions, which collectively account for 82.5% of the total variance. This plot illustrates a separation of the
entire sample according to sex and age-group category. The data separate into three clusters: the end of childhood (ages 56), the juvenile period (ages 78
to 910), and the pubertal period (ages 1112 to 1516). Sex differentiation is evident in all developmental stages with females beginning developing earlier
and completing growth much faster than males.
FIG. 6Sex differences in craniofacial growth. Females: circles with solid lines, Males: triangles with dotted lines. This two-dimensional plot represents
the first and third canonical functions, which collectively account for 76.7% of the total variance. This plot shows that although sex differences are established early in the neurocranium, the expression of sexual dimorphism in the entire craniofacial complex depends on the growth interaction between all the
craniofacial features.
30
Model
Variable
Unstandardized
Coefficients
Standardized
Coefficients
Wilks
Lambda
Canonical
Correlation
Ages 56
PPL
NSL
OBL
BOL
SPL
BSL
NPL
PNL
BBL
SOL
PSL
BNL
Constant
BOL
PPL
PBL
NBL
BNL
NOL
Constant
1.201
)0.419
0.602
0.171
0.894
)0.373
0.467
)0.712
)0.306
)0.692
)1.208
0.810
19.809
0.142
)0.170
)0.081
0.198
0.260
)0.141
)29.547
2.845
)1.057
3.262
1.117
2.381
)0.861
1.517
)2.103
)1.256
)3.288
)3.735
2.715
0.685
0.561
0.950
)0.358
)0.227
0.649
1.004
)0.781
0.609
0.625
Ages 78
Sectioning
Point
% Accuracy
Original
% Accuracy
Cross-Validated
F: 0.672
M:)0.672
F: 80
M: 76
Total: 78
F: 74
M: 74
Total: 74
F: )0.793
M: 0.793
F: 76
M: 84
Total: 80
F: 76
M: 80
Total: 78
Sectioning
Point
% Accuracy
Original
% Accuracy
Cross-Validated
Centroids
Model
Variable
Unstandardized
Coefficients
Standardized
Coefficients
Wilks
Lambda
Canonical
Correlation
Ages 910
PBR
PBL
BSL
SPL
PNL
SGL
NPL
SOL
NBL
OPL
BNL
Constant
OBL
BSL
BOL
PPL
PBL
NPL
BBL
SGL
GOL
NBL
PSL
NOL
Constant
0.466
0.542
0.542
)0.424
0.395
0.566
)0.307
0.154
)0.286
)0.184
)0.772
)25.176
0.995
0.915
0.908
0.650
0.596
0.370
)1.236
1.283
)0.688
0.922
)0.885
)0.899
)14.079
2.299
1.626
1.246
)0.972
1.097
1.762
)0.971
0.750
)1.101
)1.199
)2.908
0.621
0.615
F: )0.773
M: 0.773
F: 86
M: 78
Total: 82
F: 80
M: 78
Total: 79
5.460
2.224
7.402
1.663
2.395
1.169
)5.639
3.870
)4.138
3.883
)3.592
)5.134
0.641
0.599
F: )0.740
M: 0.740
F: 76
M: 80
Total: 78
F: 70
M: 74
Total: 72
Ages 1112
Centroids
31
Model
Variable
Unstandardized
Coefficients
Standardized
Coefficients
Wilks
Lambda
Canonical
Correlation
Ages 1314
PBR
OBL
PPL
SPL
SGL
NPL
PNL
BBL
NSL
NBL
GOL
PSL
SOL
BNL
OPL
Constant
BOL
BSL
OBL
BBL
SGL
GOL
BNL
PSL
OPL
NOL
Constant
)2.344
)0.334
0.845
0.442
)0.087
2.070
)0.306
0.727
)0.583
1.835
0.440
)0.936
)1.191
)0.494
1.056
)23.371
)0.105
0.425
)0.315
0.205
0.425
)0.448
)0.323
)0.226
0.255
0.516
)39.242
)12.842
)1.816
2.234
1.263
)0.290
7.363
)0.900
3.066
)1.864
8.326
2.843
)3.595
)6.014
)1.978
6.686
0.531
0.685
F: )0.931
M: 0.931
)0.660
1.125
)1.778
0.878
1.246
)2.709
)1.063
)0.853
1.662
3.086
0.420
0.762
F: )1.164
M: 1.164
Ages 1516
Centroids
Sectioning
Point
% Accuracy
Original
% Accuracy
Cross-Validated
F: 86
M: 80
Total: 83
F: 80
M: 76
Total: 78
F: 88
M: 90
Total: 89
F: 84
M: 86
Total: 85
32
recognition that the craniofacial skeleton has a modular organization. Sex expression and the ability to identify sex-specific traits
vary according to age-group owing to variation in growth patterns
between the neurocranium and face. Therefore, recognizing the
growth variation between the sexes allows the identification of sexual dimorphism in the juvenile craniofacial skeleton.
Investigators should approach the method presented in this study
with caution. If juvenile remains are recovered and the craniofacial
skeleton is in good shape, radiographs of the head can be taken. If an
age estimate can be derived from the dentition, then as long as the
magnification factor of the X-ray machine is known, the craniometric landmarks described in this study can be identified and the radiographs measured with calipers or some other tool. The investigator
will then have to convert the measurements to natural size and apply
the measurements to the sex classification equations presented previously. This method is simple and practical and may be of use when
genetic methods for sex identification fail to yield reliable results.
Some may argue that controlling the data for size by using techniques such as the one provided by Darroch and Mosimann (78)
will eliminate the need for breaking the sample into age-groups,
thus lumping all the age-groups together and creating a single discriminant function for males and females. However, the major
source of variation between the sexes throughout development is
size. It is through the observation of size differences that it is possible to identify important developmental events in the craniofacial
skeleton and for that matter, in the rest of the skeleton. Size is an
important source of variation that should not be ignored. By eliminating size from the data, the important components that contribute
to sexual dimorphism, at least in this sample, will be eliminated as
well. Consequently, it would not be possible to identify sex-specific
variation in the data.
The results of this investigation require careful consideration and
may require further study. Sample size, sample quality, sample representation, and sample treatment during an analysis can affect statistical outcomes. The data utilized in this study represent a very
specific population in the United States. Therefore, the results presented here may only be representative of this population (61). The
results of this study may or may not be applicable to other populations. Moreover, this study only used lateral cephalometric radiographs. As a consequence, this study does not provide a complete
profile of the development of craniofacial sexual dimorphism in
juveniles of European descent (61). We need to know more about
craniofacial growth variation between and within populations. Fortunately, there are numerous researchers presently studying the
human variation in craniofacial growth and development (79,80).
Further study is necessary to make this and other juvenile identification methods acceptable to the Daubert Standard (81). Nevertheless, this investigation contributes to the growing body of work
involving the study of sexual dimorphism in humans and should be
viewed as the first of many studies yet to come.
Acknowledgments
Conclusions
In conclusion, the results presented previously demonstrate the
presence of sexually dimorphic differences in craniofacial growth.
The pattern of craniofacial sexual dimorphism presented in this
study derives from variation in the rate and timing of growth,
which leads to allometric differences between the sexes. Females
have a faster rate of development and cease craniofacial growth at
a much earlier age than males. Sex identification depends on the
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Additional information and reprint requests:
Richard A. Gonzalez, Ph.D.
Department of Physical Therapy
Clarkson University
Clarkson Hall
59 Main St.
Potsdam, NY 13699
E-mail: rgonzale@clarkson.edu