Isolation

and

Identification

of

Amniotic

Membrane SLPI Gene

One of gingival recession treatment is through surgery which will cause a
wound. Sterile condition or free of bacteria is hard to achieve in the oral cavity. Many
methods were developed to faster wound healing. Today many wound treatment in
gingival recession with surgical procedure without active material application for
healing; the wound was only protected with periodontal pack.
SLPI is a protein which has some functions in wound healing such as protease
inhibitor, control leucocytes activity, reducing TGF beta, anti inflammatory, anti
bacterial, control intracellular enzyme, monocytes and matrix metallo proteinase
(MMP) suppressor (Zhang et al 2002).
According to Ashcroft et al (2000), SLPI reduce Nuclear Factor-kB (NFkB) a
transcription factor which can induce inflammatory gene activation. Cell signaling
molecule would be activated by inflammation process, kinase protein would be
activated and Mitogen-activated protein kinase (MAPK) would be stimulated by later
process of kappa B inhibitory (IkB) would be activated and degraded into NFkB
excessively.
SLPI is serine protease inhibitor found in large amount in bronchus, nasal,
cervical mucous, saliva, and seminal fluid (Zhang et al., 2001). SLPI is a protein with
+ 12 kDa consists of two homology rich in sistein on 53 and 54 amino acids. Inhibitor
activity to inhibit chemotripsin, tripsin, netrofil elastase and Capthesin G. Based on
above introduction hopefully the addition of SLPI amnion membrane could be
considered as biomaterial candidate to faster gingival wound healing.
SLPI recombinant is needed to get pure SLPI protein and specific which
capable as bioactive material to faster wound healing (Combe, 2007). Preliminary
studies has been done and resulting a trash protein band. The result makes pure SLPI
hard to get, besides make contamination easier, and lessen the protein amount. When
host cell divides, copy from recombinant DNA molecule inside vector would also be
carried by the host cell divisions along with the vector. Multiple divisions would

produce several host cells called identical clone and contain one or more recombinant
DNA molecule copies (Nicola and Andrew, 2005).
REFERENCES
Ashcroft D.B., Xinhua J.W., Winston H.S., John K. (2000): Inflammatory gene
activation using SLPI, Nature, 921: 53-9.
Combe E.C. (2007): Textbook of Gene Sequencing Techniques. 2 nd ed, Elsevier, New
York, p: 77-90.
Nicola K. and Andrew M.A. (2005): DNA molecule copies and host cell clones, JDR,
329: 59-67.
Zhang J., Min X., Mikhail K., Olivier G.M., (2001): SLPI gene in research use,
IADR, 329: 723-8.
Zhang M., Antonio H.G., Pinoy N., Masuya U. (2002): Amnionic membrane gene
patent, JDR, 89: 649-57.