Code.

No: 37188
R05 SET-1

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

IV .B.TECH – I SEM REGULAR EXAMINATIONS JANUARY- 2010
DOWNSTREAM PROCESSING
(BIO-TECHNOLOGY)
Time: 3hours Max.Marks:80
Answer any FIVE questions
All questions carry equal marks
---

1. What are the salient features, advantages and disadvantages of bioprocess

compared to conventional chemical process? [16]

2. What are the stages involved in the recovery of intra cellular products? Explain
with help of suitable unit operation involved in each stage. [16]

3. Write short note on the following:

a) Cell aggregation.
b) Flocculation.
c) Rotary vaccume filter. [16]

4. Write short notes on.

a) Liquid membranes
b) Ultra filtration [16]

5. Write short notes on

a) Precipitation of proteins by organic solvents.
b) Enrichment operations by polymers. [16]

6. Write short notes on.

a) SDS – gel electrophoresis
b) Staining techniques for protein gels. [16]

7. Describe the bio specific affinity chromatography and its industrial applications.
[16]

8. Describe the principle, types of crystalisation and its significance in product

recovery. [16]

*****
Code.No: 37188
R05 SET-2

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

IV .B.TECH – I SEM REGULAR EXAMINATIONS JANUARY- 2010
DOWNSTREAM PROCESSING
(BIO-TECHNOLOGY)
Time: 3hours Max.Marks:80
Answer any FIVE questions
All questions carry equal marks
---

1. Discuss about the application of biochemical engineering principles in

downstream processing? [16]

2. Discuss the steps involved in product isolation and purification of an enzyme?

[16]

3. Write short notes on the following.

a) Settling velocity in a centrifuge
b) Sigma factor of a centrifuge
c) Tubular centrifuge. [16]

4. Explain in detail how do you classify the membrane separation methods

according to particle size? [16]

5. Write the advantages and disadvantages of using ionic and non-ionic poly
electrolytes for enrichment operations. [16]

6. Write the principle of pulse field electrophoresis. Write the method and
significance of pulse field electrophoresis? [16]

7. Describe the principle, advantages and disadvantages of gas chromatographic

detectors. [16]

8. Write short notes on.

a) Super critical fluid extractions
b) Differences between precipitation and crystallizations. [16]

*****
Code.No: 37188
R05 SET-3

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

IV .B.TECH – I SEM REGULAR EXAMINATIONS JANUARY- 2010
DOWNSTREAM PROCESSING
(BIO-TECHNOLOGY)
Time: 3hours Max.Marks:80
Answer any FIVE questions
All questions carry equal marks
---

1. What do you understand by upstream and downstream processing? Discuss with

neat flow charts. [16]

2. a) Discuss recent developments in product isolation.

b) Describe characteristics of fermentation broths. [16]

3. Write notes on the operation of tubular bowl centrifuge and disc stack bowl
centrifuge. [16]

4. a) What are the advantages of membrane separation processes

b) What is Ultra filtration? How is it useful in bio separation? [16]

5. What is integrated bio processing? Explain it with a familiar industrial product

as an example? [16]

6. Write the principle, instrumentation, detection and significance of capillary

electrophoresis. [16]

7. Write short notes on:

a) Partition coefficient
b) Retention time
c) Retention volume
d) Band broadening. [16]

8. Discuss the process of crystallization. What are the different parameters

considered before crystallizing a compound. [16]

*****
Code.No: 37188
R05 SET-4

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

IV .B.TECH – I SEM REGULAR EXAMINATIONS JANUARY- 2010
DOWNSTREAM PROCESSING
(BIO-TECHNOLOGY)
Time: 3hours Max.Marks:80
Answer any FIVE questions
All questions carry equal marks
---

1. Describe about economic assessment of various proteins via –r – DNA. [16]

2. Discuss the steps involved in the product isolation and purification of ethanol
fermentation [16]

3. Write notes on the operation and functioning.

a) Bead mill
b) Homogenizer in cell disruption. [16]

4. Discuss about the basic principle of membrane separation and explain about
different membrane configurations. [16]

5. Explain “in situ product removal” as a tool for bio processing? [16]

6. Explain the various steps along with the principle of 2D-gel electrophoresis.
[16]

7. Discuss the qualitative and quantitative applications of gas chromatography.

[16]

8. Write the principle of super critical fluid extraction, phase diagram and
advantages of super critical fluid extraction. [16]

*****