Certificate of Analysis

RAP (human) (recombinant)

Product Number: BML-SE552
Lot Number: 12211215
Purity: >90%
Method (purity): SDS-PAGE
Concentration: 0.32 mg/mL
Endotoxin level: <0.31 EU/mg
Long Term Storage: -80C
Formulation: Liquid. In 0.0067M Phosphate, pH 7.4 containing 0.15M
sodium chloride.
Handling: After opening, prepare aliquots and store at -80C.
Avoid freeze/thaw cycles.
Source: Produced in E. coli.
Country of Origin: USA

By:
Name: Eric Begley
Title: Operations Supervisor
Date: 10/14/2013

10/14/2013

General Product Storage and Handling Information

Specific storage and handling information for each product is indicated on the product datasheet. Reagents have a
warranty of one year from the date of receipt, except for reagents with an expiration date indicated on the label
or other supporting document, and conjugates and proteins which are warranted for six months. Most Enzo
Life Sciences products, stored under the recommended conditions, are stable for at least one year. Products are
sometimes shipped at a temperature that differs from the recommended storage temperature. Many products are
stable in the short-term at temperatures that differ from that required for long-term storage. We ensure that the product
is shipped under conditions that will maintain the quality of the product, but save you shipping charges by using the
most economical storage conditions for an overnight shipment. Upon receipt of the product, follow the storage
recommendations on the product data sheet.
Solubilization of Small Molecules
Solubility information can be found on the product datasheet. Concentrations listed are concentrations at which
products have been tested for solubility by Enzo Life Sciences. If a solvent, but no concentration, is listed, the product
should be soluble at typical stock concentrations. Make sure any organic solvents used are anhydrous. Unless stated
otherwise, solutions can be warmed in a water bath to improve solubility. For very hydrophobic products, the addition
of a carrier protein, such as BSA, to the aqueous media may also be helpful.
Using water or other aqueous media to make serial dilutions of compounds dissolved in organic solvents is not
recommended. Rather, make a concentrated stock solution in an organic solvent and dilute directly to your working
concentration in the appropriate aqueous medium. Some products may precipitate upon initial dilution, but warming the
solution will often redissolve the precipitate. Some hydrophobic compounds can be effective in tissue culture
experiments when used in suspension rather than in solution.
Solubilization, Handling and Storage of Peptides
For maximum stability, store lyophilized peptides desiccated at -20C. If the peptide is to be weighed out, equilibrate to
room temperature in a desiccator prior to opening. Adsorption of water reduces stability and affects sample weight.
Changes in temperature during the defrost cycle in a frost-free freezer may reduce peptide stability. Reconstitute
peptides in sterile, distilled water. A few drops of ammonium hydroxide for acidic peptides or 10% acetic acid for basic
peptides will facilitate dissolution. If necessary the solution can be sonicated briefly. With extremely hydrophobic or
neutral peptides, solubilize using a minimal amount of dimethylsulfoxide (DMSO) or dimethylformamide (DMF) then add
water or buffer. In order to avoid serious solubility problems, fully dissolve peptides before adding buffer or saline. Use
oxygen-free solutions and reducing agents with peptides containing Trp, Met or free Cys to avoid oxidation.
After reconstituting, aliquot the solution into individual tubes and freeze at -20 or -70C. Avoid repeated freeze/defrost
cycles. In solution, store peptides at pH 5-7 for maximal stability. Peptides in solution have limited stability, especially
peptides containing Cys, Met, Trp, Asn and Gln. For maximum stability re-Iyophilize reconstituted peptides.
Enzymes
Enzymes must be handled particularly carefully In order to retain maximal enzymatic activity. Defrost enzymes quickly
in a room temperature water bath or by rubbing between fingers, then immediately store on an ice bath. Unused
enzymes should be quickly refrozen by placing at -70C. To minimize the number of freeze/thaw cycles, aliquot
enzymes into separate tubes and store at -70C.
Lipids
Lipids can be supplied either dissolved in solvent or neat (not dissolved in solvent). Lipids supplied neat are sold by
weight and appear as oily liquids or waxy solids. To use, add a known volume of appropriate solvent, divide the weight
of the lipid supplied by the volume of the solvent added to obtain the concentration. Aliquot as needed and store the
remaining product as recommended on the datasheet.
Antibodies
Antibodies have a compact and stable secondary structure making them relatively stable proteins. Specific storage
recommendations are listed on the datasheet. In general, antibodies can be stored at -20C or -70C. Avoid repeated
freeze/defrost cycles. Aliquot undiluted antibody into smaller volumes (not less than 10 L) prior to freezing if
appropriate. Store diluted antibody at 2-4C (do not freeze) and use within 1 month.

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Species cross-reactivity and tested applications for each antibody are listed on the datasheet.
Germany

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If you need additional information or technical assistance with any of our products please contact us at:
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WARNING: THIS PRODUCT IS NOT INTENDED OR APPROVED FOR HUMAN DIAGNOSTICS OR VETERINARY USE. USE OF THIS PRODUCT FOR
HUMAN OR ANIMAL TESTING IS EXTREMELY HAZARDOUS AND MAY RESULT IN DISEASE, SEVERE INJURY OR DEATH.

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MATERIAL SAFETY DATA: This material should be considered hazardous until information to the contrary becomes available. Do not ingest, swallow or
inhale. Do not get in eyes, on skin, or on clothing. Wash thoroughly after handling. This information contains some, but not all, of the information required for
safe and proper use of this material. Before use, the user must review the complete Material Safely Data Sheet.
WARRANTY AND LIMITATION OF REMEDY: Enzo Life Sciences AG, Enzo Life Sciences International, Inc., Enzo Life Sciences GmbH, Enzo Life Sciences
(UK) Ltd. and Enzo Life Sciences BVBA ("The Companies") make no warranty of any kind, expressed or implied, including, but not limited to, the warranties of
fitness for a particular purpose and merchantability, which extends beyond the description of the products on the face hereof, except that the material will meet
our specifications at the time of delivery. Buyers exclusive remedy and the Companies sole liability hereunder shall be limited to refund of the purchase price
of, or at the Companies option, replacement of all material that does not meet our specifications. The companies shall not be liable otherwise, or for Incidental
or consequential damages including, but not limited to, the costs of handling. Said refund or replacement is conditioned on Buyer giving written notice to the
Companies within thirty (30) days after arrival of the material at its destination. Failure of Buyer to give said notice within thirty (30) days shall constitute a
waiver by Buyer of all claims hereunder with respect to said material.

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