)~

Proceedings

The Joint Meeting of Conference and Congress

of Asian Society of Veterinary Pathology

(ASVP) 2011
&
th

The 10 Scientific Symposium of Indonesian

Society of Veterinary Pathology (lSVP) 2011

The Role of Veterinary Pathology Animal Health for

Improving Eco-Health

IPB International Convention Centre

Bogar, Indonesia, l'Jovember 2011

Hosted by Faculty of Veterinary Medicine

Bogar Agricultural University 2011

Faculty of Veterinary Medicine

Bogor Agricultural University 20 I I

All right reserved. No part of this book may be reproduced in any form without
pennission in writing from the publisher, except by reviewer who wishes to quote brief
passages in review written for inclusion in magazine or newspaper.

Perpustakaan Nasional Indonesia Cataloguing -in-Publication Data

The Joint Meeting of the 5th Conference and Congress of Asian Society of Vetrinary
Pathology (ASVP) and the 10th Scientific n Symposium of Indonesian Society of
Veterinary Pathology (ISVP), 2011,
The Proceedings of the Joint Meeting of the 5th Conference and Congress of ASVP and
the 10th Scientific n Symposium of ISVP, November 22-24,2011,
IPB International Convention Centre, Bogor Indonesia.
ISBN; 978-979-493-371-8

Typest and Printed by

Penerbit IPB Press
Contact Address
ASVP 2011 Organizing Committee
Division of Veterinary Pathology
Department of Veterinary Clinic, Reproduction & Pathology
Faculty of Veterinary Medicine
Bogor Agricultural University
Wing 6th & 7th, 1st Fl. .II. Agatis, Campus IPB of Darmaga
Bogor 16680, INDONESIA
Phone/Fax. +62-251-8421807
E-mail ;asvpbogor2011@gmail.com
Website: http://asvpbogor201I.event.ipb.ac.id

"

Page

Invited Speaker
OPS 1

TGF-beta Niche Regulation for Better Therapy

jin-Kyu Park! , EUI1-lv[i Lee l !, Ah- Young Kin/!, Sang- Young

You!!. Seon-Yolll1~ HOI/! . Elin-Joo Le/ c, and Kl'll-Shik

Jeong l :'
'

OPS:2

OPS 3

OP54

OPS 5

OPS 6

OPS 7

5
Pathological findings and Molecular charcteristics ofPRRSV

isolated from infected in North of Vietnam

Ng/lyen Thi Lanl. Nglll'en HUll Na1/1 L. Ngl;yen Ba Hierl Le ,

Huynh Thanh P/lIIongL Ngllyen Thi Hoa~. Ryoji YOll1agllchi

8
Rat Tumor Models and their Applications, Pariicularly

for Renal and Hepatic Fibrosis

Jyoji Yalllate
II
Vascular Pathology Associated with Experimental
Pastellrel/a IIlItltocida serotype: B:2 Infection in Calves
S. J(lSni. E.lY1. AlIIl1(l. At Z(JI/Iri-Sa(/d. Z Zakario, S.S. Arslwd.
A. R. Ol/wr and T I. A:::llli
13
Challcnges in safety evaluation ofbiomaterials. biomedical

devices and engineered tissue constructs

TV Ani/kllmar. R Deepa (ll1d Al Jaseer

15
Diagnostic Classification and Detection of Minimal Residual
Disease (MRD) in Canine Lymphoma
~Blll1gsipipot, N :\4wwc//(/i. J Clwyapollg, S. Wangl1uifhol/l

ond S. TechollgulIIs/l\\'(//i

20
The Development of Porcine Reproductive and Respiratory

Syndrome Virus Vaccine

Min- Yuan Chid', Hui- Ting Chan", Yi- Yin Do",

Pllng-Ling Huang". Victor Fei Pang", Chian-Ren

Page

Participant
POI

P 02

I' 03

25

Pathology and Veterinary Pathology Challenge-Future

Pcrspective
1.1. A/ SlIlfatl
Ultra structural Characteristic of Blood Cells in Giants
Freshwatcr Stingray (Hil//(/lIfllnt clwop/iruya)

A.Sailasl/to. Y. ;\Iat/lIJra, N. CI/(/I1SII(,

Analysis of 1'53 and PCNA Expression in the Genital and

Extra genital CTVT Microarray

S. Jon/alltsa. K. T(lIIgfrogoon. P. Kli%fha, S. Bolflwisollf!,.

S. iVallgnait!II//II. P. TO/lgkOll'Olfana, A. Rl/ngsipl/)(It. S.

26

28

To ngkOlI'atto 110

P 04

30

Comparing Expression ofHERR-2 in Malignant Feline

Mammary Tumors
Tissue Microarray

tV RlIngrass(/l/Iee. P. Tml'Ornpanich. W. Jilltowes, S.

Bulfhaisong, S. Wal1gnaifhlll1l. P. Tangkmraffmw. A.

I' 05

Rllngsipipai, S. TClllgkall'affona

Investigation of the Death of Java Rhinoceros (Rhinoceros

sondaicus) in Ujung Kulon National Park

Adhi Racl1llI(Jf Slidraiat Har~l'Odi. Halldayani, Agl/s

Pr~l'OlIIhlldi,

I' 06

Ridlmn Seti(JlI'{I/I

Pathogenicity of a Pathogenic Filed Isolate NDV/Bali- 1107 in

Commercial Chicken Strain ISA brown
Anak Agllng Ani /vliwh Adi, iHade Kardel1a. !~ro/llall ,Hanfik

Asfmi'(J Yoshihiro Hayashi. YaslIl70hll AlotSl/llloto

35

P07

P 08

P 09

PIO

PI!

PI2

P 13

PI4

P 15

P 16
PI7

PI8

P 19

P 20

P 21

Immunohistochemical Characterization of Macrophages and

Myofibroblasts in Biliary Cirrhosis due to Liver Fluke
(Fasciola spp) Infestation in Cattle
HM Golbar. V Junianfifo. C. Ichikawa. M. Tanaka. T. [::.m!'a.
Af KlIlI'amlira. OJ. 'lamale
Expression of Metallothionein Protein in Malignant Feline
Mammary Tumors by Using Tissue Microarray
P. Siffhicharoenchai. P. Alarlow. P. Prufthifhmrorn. S.
Sirivaidyapong. S Wangnaithllll1 . . S Tangkall'utfana, A.
RUl1gsipiput
Genetic Characterization and Phy logenetic Analysis of Thai
Canine Distemper Virus (CDV) Isolates
S TechangamslI\\'{ln, N Charoensiml. A. Radranakatikanol7.
A. RlIl1gsl/JI/)(It
In Vitro Antimicrobial Activity of Saccharomyces cerevisae
Against Streprococclis agalactiae Isolated from Nile Tilapia.
K Pinpill1ai. P. Kaywlsalllrlwj. C. Rodkhwl1. A. POl1pornpisit.
LV. pirarat.
Necrotizing Granulomatous Lesions of Uterus in a Dog by
Nematode Larvae in the family ofOnchocercidae
JK Park A Y Killl, El'" Lee. l.:.J. Lee, SY. YOII. SY. Han. DAf
KHak. TH Kim. KS. Jeong
Phylogenetic and RFLP analysis of Canine Distemper Virus
Fusion (F) Gene from Domestic Dogs in Thailand
A. Rac/tanakatikanon. N. Clwroensivu/. J. Kemrchoroen. K.
Oraveeraklil. K Sliwo/lnokaril. r Poovorowo!1. S
Techongwl/s 11\\'(//1
Immunohistochemical Detection of Macrophages and
Myolibroblasts in rat Model of Bleomycin-Induced
Scleroderma
Vetlli::.ah Jl/lliO/lto. Cliisa [chikm\'(/. Takeshi 1::'<7\m. lv[itslirII
Kill \'(/ 11 /(/I'll. Jroji 'l(II 11 ate.
High Pathogenic Avian Influenza Infection in Wild Birds of
Korea.
Olin-Kl'Ong 1"'0011. Woo-Hee Park KYlll1g-f~\'IIn Lee. In-Soon
Rooh. Moo/1- Yo ling Rhyoo. Hl'e-Ryoung Kim. YOII Chan Bae.
O-Soo Lee
Ganglioneuritis of Indonesian Abalone
Dewi Ratill AglIllgprimno. Mm\'aI'Sllballgkit. Agus SlIl1al'to
Canine Multiple Epithelioid Hemangioma in Labial Mucosa
Elv!. Lee. A Y. Kil1l. EJ. Lee. BS. Joo. SY. YOII. SY. Han. YH
YOOI1. KS. Jeong
Histopathological Changes and Apoptosis Detection in Canine
Myxomatous Mitral Valve Disease Using Tissue Microarray
Technique
T. Jiranantasak, A. Rungsipipat, S. Disatian
Case Report: Infectious Canine Hepatitis and Colibacilosis in
Malayan Sun Bear (He/arc/os Afalayanlls)
Agustin [ndrm\'afi
Generation of Hepatocellular Carcinoma animal Model in
Sparoglle Dmrfey Rats
Aglls Setil'Ol1o. [ndriayzmi Prahastllti. Elpita Br Tarigall.
fJkterono Dlt'i BlI(Zvati. Andi [llama
Case Report: Ovarian Lipoma in a Dog
JH Park A Y. Kim. EJI Lee. sr. Yo II. SY. Han. EJ. Lee. BS
.100. AI Mlln. I'll.. Yoon, KS Jeong
Decreased Myostatin Moves Up Fetal Myogenesis in Somatic
Cell Nuclear Transferred Dog
EJ. Lee, JK. Park. A Y. Kim. EAt Lee. Sr. ron. sr. Hall. YH
Yoon. BS .100. JH Park. AI. AlII, KS Jeong

37

40

43

46

48

51

54

56

57
59

62

65

67

70

72

P 22

Ceruminous Adenocarcinoma with Psammoma Bodies in

External Auditory Canal of a Dog

75

A Y Kim, E/v1 Lee, SY YOIl, SY Han, YR. Yoon. KS Jeong

P 23

Production and Characterization of Plyclonal Anti Domain-I

Alpha-Fetoprotein Antibody

78

AglIs Setiro/lo, Elpita BI' Tarigan, IlldriaYlini Pralwsfufi. ,

Akferono Dwl Blldmfi, Andi Ufallla

P 24

Pesticide Poisoning in Korean Wild Birds from 2008 to 20 II

81

P 25

OIlI1-Kl'o/lllg Moon, Moon- rOllng Rhyoo, Kyung-Hl'lIl1 Lee, In

Soon Roh. Woo-Hee Park, Dong-GYII Kim, Jin- YOllllg Shin,
HYIlI1-Jeong Kl1'li/l, Clwe-III; Lim, O-Soo Lee
The Effect of Blackseed (Nigel!([ satim) Oil Extracts on Mice

82

(Mus musculus) Testicle Histopathology,

A,

P 26

Rahllli, D,R, AglI!1gpr(I'0I10, S Estllnlllgsiit

Splenic Angiomyxoma in a Dog: The First Reported Case

BS, Jo, A Y Kim, Elf Lee, SY YOll, SY. Hcm, EJ. Lee, JR.

85

Park. AL MIIII, YR. YOOII, KS Jeong

P27

A Case of Hemangiosarcoma in a Jindo Dog

H7

AL M'III, A Y. Kim, EM Lee, SY. Yo II, St'. Han. fJ Lee, BS.

JO(), JR. Pork. KS Jeong

P 28

Foot and Mouth Disease Virus Infection in Young Korean

Black Goat (Capra itirclIs) in Korea
KI'II/Ig-Hmn Lee, In-Sool1 Roil, Woo-Hee Park. MoolI- YOllllg
R!tl'Oo, YOlIlIg-JOOII Ko, JOllg-Hl'eoll Pork, OIlIl-KnJllng Moo/l,
O-Soo Lee

P 29

Acute Severe Melena Caused by Salmonella Paratyphii C

Infection in Javan Slow Loris Nycticebus Javanicus (A Case
Report)

90

[//fall Citronillgplltri, S//(/rlllilli JP, Kat/"e/e {S, rVendi P

P 30

P 31

A Case of Bacterial Septicaemia in a Malayan Tapir (Tapirlls

indiclls)
Sr/\'(/lIlo, S, S//(//,il"lldill, :'vI. ZWllri-Saad
Effect of Garlic (Allilllll sol/mill L) to the Immunity of Carp
Fish (Cl'prillllS (,lII1)io) Infected by EdH'ordsiel/a tonfa
Kllmi({sih, Sya/'itiu/din Toto

The Application of Immunohistochemistry to Detect Prion

Protein (Prp) of B(m'lIe Spongitiml/ El7cepjaiopafhy (BS E) in
Feedlot Cattle to Anticipate BSE Emergence in Indonesia

92

94

96

Rilli /)unwyanti

P 33

Mammary Adenocarcinoma with Pan-Cytokeratin and Alpha

Estrogen Recepto Immunohistochemical Detection in Captive
African Hedgehog (AtelerJ~,( a/hit'ellf!'is)

98

K(/tr~\'a ClwnkOlI', KOlllkiell' Pinpimai, Poison Tiellt/wi,

Nopadul1 Pirarof.

P 34

The Suspect Atlatoxicosis in Toxicity Case Caused by

Aspergillus Fungi

P 35

Gross Pathology Report of a Wild-Born Captive Sumatran

Rhino (Dicerorhinlls Sli 17 W frellS is ), Torgamba
DR. Agul7gpr~l'OlIo, AI Suhangkil, S. EStlll1illgsih, D, Condra,

P 36

Pulmonary Lesions of an Aged Sumatran Rhinoceros

WohYllni, Hadi

100

PUrl/WIIO ~f/,

102

4ndricll1syah

105

Wilrin Wil7o!'sih, Vetni::ah Junianto, DeH'i Rati"

Agungpn)'Ono, Dac/wl D. SlIbrata.

P 37

Widespread Fibrosis Due to Uremia in an Aged Sumatran

Rhinoceros (Dicerorhinlls slIl1Iatrensis)
Vetni:;ah JlIl1ianfo, Aglis Selil'Ono, BalJlbong Pontjo
Priosoer}'anto, Al1drial1s~'{/h

107

PANCREATIC BETA CELLS EVALUATION AFTER TREATED

BY Plwleria macrocarpa (Mahkota Dewa) FRUIT EXTRACT IN DIABETIC MONKEY
E. Sulistiawati\ I. H. Suparto l 2 , M. Bintang 1, 1. Indraswaril, S.A. Prabandari

Iprimate Research Center, Bogor Agricultural University

'Department of Chemistry and 3Department of Biochemistry Faculty of Mathematics and Natural Science,

Bogor Agricultural University

Keyword: Phaleria macrocarpa, Diebetes Mdlitus. ,\/acaca/ascicularis

Introduction
Phaleria macrocarpa, is a medicinal plants
originated from Papua. Empirically, it is capable to
control \arious health problems including diabetes
mellitus. There is growing evidence that excess
generation of highly reactive free radicals largely
due to oxidative stress (hyperglycemia) causing
increase blood level. This further exacerbates the
development and progression of diabetes and its
complications. Based on previous studies, Phaleria
macrocarpa contained antioxidant of phenolic
glycoside (Oshimi, et al., 2008) and lignans
pinoresinol, lariciresinol, and matairesinol (Saufi et
aI, 2008). The aim of this study was to evaluate the
numhcr of heta cell pancreas and to detect antigen
antihody reaction of ~ cells after treated Phaleria
macrocarpa fruit extract in diabetic monkey (DM)
induccd with streptozotocin (STZ).
Materials and Methods
Pancreatic tissues from fifteen diabetic adults
male Macaca fascicularis were collected at
necropsy and preserved in 4% paraformaldehyde as
fixative solution. Each pancreatic tissues were
trimmcd at three difTercnt areas; caput, corpus and
cauda,
processed
for
immunohistochemical
staining, then evaluated and calculated under
microscope. Monkeys werc induced by single
intravenous injection of STZ (55 mg/kg BW) to be
DM. All DM were divided randomly into threc
groups (n = 5 anilllals). First group, DM treated
only with distilled water as control, second and
third groups were treated with Phaleria
macrocarpa fruit extract of 1000 and 500 mg/kg
BW, respcctively. All experimental procedure on
theses animals were conducted in compliance with
the guideline estahlished hy the Institutional
Animal Care and Use Committee.
Results
Pancreatic l3 cells of the Langerhans Islets
distributed randomly in three different areas, caput,
corpus, and cauda pancreas. The mean of number l3
cells on the caput pancreas of the control DM was
higher than both of treated DM group. While the
mean of the number l3 cells on the corpus and cauda

pancreas of DM which had received extract of 500

mg/kgBW was higher than the control and animals
with 1000 mg/kgI3W (Figure. 1).
Immunohistochemical staining method showed
various color intensity which depends on the
concentration of antigen-antibody binding, tissue
preparation and other factors. In this study, the
hrown color intensity indicated the amount of
insulin that sccreted by pancreatic l3 cells. Factors
afTccting the color intensity of the antibody-antigen
binding specificity closely related to the
concentration of primary antibody used in the
process. Optimization of anti-insulin concentration
was performed and revealed that the concentration
of I: 1500 \\'as the optimal concentration with
unstained background. supported by Gobel (20 II).
I3ascd on the data of the color intensity of
pancreatic ~ cell evaluation, the caput pancreas of
the control DM had the lowest of color intensity
compared to both treated groups. Corpus and cauda
areas of animals treated with 500 mg/kg BW
showed highcr intensity compared to the other
groups (Figure 2).

Disscussion
Detection of pancreatic l3 cells in Langerhans
Islet hy immunohistochemistry indicate the
presence of ~ cell damage in diabetes mellitus.
Pancreatic tissue of untreated animals had lowest
pancreatic l3 Ct~11 compared to treated animals. This
may he caused hy ad\'anced ~ cell damaged due to
cytotoxic cffect of STZ. Glucose uptake through
glucose transporters (GLUT-2) caused the STZ into
~ cdls, resulting in DNA alkylation. DNA was
fi'agmented so that activating poly (ADP-ribose)
synthetase, the enzyme that polymerizes to form
the ADP-rihose poly (ADP-ribose), and activation
uf the ATP and NAD+ reduction. Decreased
production of ATP and NAD+ led to the opening of
K+ channels and the plasma membrane
hyperpolarization. Furthermore, closure of the gate
voltage reduced Ca2 + concentration and insulin
secretion resulting in l3 cell death (Elsner et al.
2000).

Caput
Corpus
'" Cauda
Control

Figure I

1000
Group

500

Mean of the number of p cells on

three different areas of pancreas by

immuno histochemical staining

evaluation

Fruit extract improved total number of p cell,

modestly higher in the lowest dosage (500 mglkg
BW). This was supported by Supa-rlo CI 01. (20 I 0),
that Phaleria macrocarpa tj'uit e;;tract increased
insulin secretion on diabetic cynomolgus monkeys.
This fruit extract was already available in the
market with dosage of 500 mg/capsule. It revealed
that dose of 500 mg/kgBW was more appropriate
to improve hyperglycemic condition or to reduce
blood glucose leve!'
Pancreatic p cells distribution in three different
areas of untreated animals showed that the caput
and the corpus pancreas had higher p cells than the
cauda pancreas. In the highest dosage, the corpus
area showed higher numbers of P cells than in the
caput and caudal pancreas. Total number of p cells
for the lowest dosage showed higher number in the
corpus and the cauda compared to caput pancreas.
This result 'was supported by Oahmiarti (2000), that
the distribution of diabetic pancreas p cells located
at the periphery of thc islets of Langerhans.
However, according to Sundler and Harkanson
(1988), P cell distribution diflcred from each
species and the composition of the islets of
Langerhans dif1ered from each area of the
pancreas. The Langerhans islets located in the
cauda pancreas seemed II) has tendency to be more
numerous.
The color intensity of pancreatic p cells were
highest in treated animal with 500 mg/kg BW. This
result was also indicated by increasing in the
number of pancreatic P cells. J\ dark brown color
on the Langcrhans Islets was resulted from the
aflinity of anti-insulin with high insulin and vice
versa. Affinity of antibody was the strength of the
bond of a side of antibody binding (paratop) with
antigenic detenninants (epitopes) (Vara 2005). So,
it can be stated that the number of pancreatic ~ cells
,vas directly proportional to the color intensity
obtained from the reaction of antigen and antibody
binding by immunohistochemical staining methods.

Conclusion

Phaleria marcocarpa fruit extract with dosage

of 500 mglkgBW orallY was the best dose to
improve pancreatic beta cells damage in DM.

.-5'
a
.5'"

Caput

corpus
IliiCauda

5
1
"0
u
0

Control

'r~--~r~""'-~

1000
500

Group

Figure 2 Mean ofthe color intensity of P cells

on three different area of pancreas

by immuno histochemical staining

evaluation

Acknowledgement
This study was partly supported by the Ministry
of Research and Technology fund (RT2009-1388)
and partly by internal research funds of Primate
Research Center of Bogor Agricultural University.
References
Elsner M. Guldbakke B. Tledge M, Munday R,
Len:aen S. 2000. Relative importance of transport
and alkylation for pancreatic beta-cell toxicity of
streptozotocin. Diabctologia.43: 1528-1533.
Gobel S. 20 II. Optimasi anti-insulin pada sel ~
pancreas
Macaw
fascicularis
secara
imunohistokimia [Laporan]. Bogor: Program
Diploma, Institut Pertanian Bogor.
Harmanto N. 2003. Afahkola Dcwa Dbat Pusaka
Para Dewa. Jakarta: Agromedia Pusaka.
Oshimi, S., Zaima, K., Matsuno. Y. Hirasawa, Y.,
Iizuka, :r, Studiawan, H., Indrayanto, G., Zaini,
N.C., Morita, H 2008. Studies on the constituents
from the fruits of Phaleria macrocOlpa,
Nat Med(Tokyo), 62(2), pp. 207-210
Saufi, A, von Heimendah!. c.B., Alfermann,
AW., Fuss, E. 2008. Stereochemistry of lignans in
Phaleria macrocarpa (Schell) Boer!. Z
Nalurforsch, 63(1-2), Pl'. 13-16
Suparto IH et at 2009. Pha/aia macrocarpa fruit
extract as insulinotropic agent in streptozotocin
induced diabetic cynomolgus monkeys (Macaca
fascicu!aris).
Oi
dalam:
Proceeding
of

International Conference on Medicinal Plants,

Surabaya, 21-22 Juli 20 I O.
Sundler J, Hakanson R. 1988. Handbook of
Chemical Neuroanatomy Vol: 6. The peripheral
Nervous System Elsevier Science Publisher BV.
Vara JAR.
2005.
Technical
Aspects of
Immuohistochemistry.
[Terhubung
Berkala]
http://vet:sagepup.com!contentl42/4/405 [03 Mei
20 II J.