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INTRODUCTION
Over the past decade, several scientific advances and economic
pressures have driven the need for improved drug discovery
screening technology
Rapid progress in genomics has greatly expanded the number of
potential therapeutic targets.
Combinatorial chemical synthesis has generated large numbers of
compounds with the potential for pharmacological activity. H
High Throughput Screening (HTS) emerged as a way to screen these
large chemical libraries against multiple targets while
simultaneously reducing development and operating costs.
In terms of definition, HTS can be considered the process in which
batches of compounds are tested for binding or biological activity
against target molecules.
Today, many pharmaceutical companies are screening 10
Today, many pharmaceutical companies are screening 100,000300,000 or more compounds perscreen to produce approximately
100-300 hits.
This is achieved using automated, robust miniaturized assays. The
use of 384-well and higher density plates and commercially
available plate-handling robotics has made HTS a reality
Several recent developments have led to new opportunities for the
use of high throughput technologies at numerous stages of the drug
discovery process.
A typical HTS system has 7 essential components: targets,
assays, compound libraries, automation, information systems,
facilities, and scientists
TARGET
Suitable facilities for the system are also an absolute necessity. This
includes adequate lab space and environmental control
Finally, Scientists are needed to design the experiments, run and
manage the system, and interpret the results