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State the resolution and magnification that can be achieved by a light microscope, a transmission electron microscope
and a scanning electron microscope.
Maximum
Resolution
Maximum
Magnification
2.
Light Microscope
0.2 micrometres
TEM
0.0001 micrometres
SEM
0.005 micrometres
X 1500
Over x 1,000,000
3.
Explain the need for staining samples for use in light and electron microscopy
In Light microscopes and TEMs the beam of lights/electrons pass through the object,
and there is an image produced as some parts of the specimen absorb more
light/electrons than others, but sometimes the specimen is transparent so it will look
white because light/electrons pass through so the object is stained
Light Microscope
Dye- usually methylene blue/eosin
4.
Electron Microscope
Specimen dipped in metal like lead, the
metal ions scatter electrons to contrast.
Emily Summers
Picture
Description
Function
Flattened membranous
sacs called cisternae,
rough is studded with
ribosomes, smooth is not.
Site of aerobic
respiration, ATP is
produced.
Site of photosynthesis,
carbohydrate molecules
made.
Emily Summers
Spherical sacs
surrounded by a single
membrane, with no clear
internal structure.
Contains enzymes.
TINY.
Bound to ER to make
RER and also in
cytoplasm. Consist of two
subunits.
Small tubes of
microtubules. A pair can
be found next to the
nucleus in animal cells.
Also in some protocytists.
Support, movement.
E.g. Chromosome
movement in mitosis.
Emily Summers
In eukaryotic cells it
contains organelles, in
prokaryotic cells it
contains enzymes
needed for metabolic
reactions.
Genetic instructions
A thick polysaccharide
layer outside of the cell
wall
Plasmid
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A tightly-folded area of
the cell membrane
Contains membrane
bound proteins needed
for respiration
6.
Explain the importance of the cytoskeleton in providing mechanical strength to cells, aiding transport within cells and
enabling cell movement.
7.
Compare and contrast, with the aid of diagrams and electron micrographs, the structure of prokaryotic cells and
eukaryotic cells.
Prokaryotic Cells
Prokaryotic cells are smaller (0.2-2.0 m)
Dont have a nucleus, DNA floats free in
cytoplasm and is circular
Less organelles and no membranous ones
70S Ribosomes
8.
Eukaryotic Cells
Eukaryotic cells are bigger 10-100 m
DNA is protected in nucleus and is linear
Many organelles, plant & animal
80S Ribosomes
Compare and contrast, with the aid of diagrams and electron micrographs, the structure and ultrastructure of plant cells
and animal cells.
Emily Summers
Cell
Membranes
9.
Describe, with
the aid of
diagrams, the
Emily Summers
13. Explain the role of membrane-bound receptors as sites where hormones and drugs can bind.
Explain what is meant by passive transport (diffusion and facilitated diffusion including the role of membrane proteins), active
transport, endocytosis and exocytosis.
Emily Summers
16. Recognise and explain the effects that solutions of different water potentials can have upon plant
and animal cells.
Emily Summers
Emily Summers
Humans have 46 chromosomes in total 23 pairs. One chromosome in each pair comes
from the mother, and then the other comes from the father. Same size, same genes
although they can have different versions of the genes (alleles).
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Meiosis:
1. Gametes are found in all
sexually reproducing
organisms
2. Male & Female join at
fertilisation forming a
zygote dividing into a
new organism
3. (Sperm and Egg)
4. (Pollen grains and
ovules)
5. Normal body cells of
plants and animals have
diploid (2n) number of
chromosomes, each cell
contains two of each
chromosome from each
parent
6. Gametes have the
haploid number of
chromosomes (n)
theres one copy of
each chromosome
7. At fertilisation the
haploid male gamete
and female fuse to
make a cell with the
diploid number of
chromosomes, half from
sperm half from egg.
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Emily Summers
Define the term stem cell
Stem cells are cells that are not specialized and can differentiate into specialized cells
with mitosis and the correct stimulation.
Define the term differentiation, with reference to the production of erythrocytes (red blood cells) and neutrophils derived from stem
cells in bone marrow, and the production of xylem vessels and phloem sieve tubes from cambium.
Bones are living organs containing nerves and blood vessels, and the main bones have
marrow in the middle, adult stem cells divide and differentiate to replace worn out
erythrocytes and neutrophils to fight infection.
In plant cells stem cells
are in the cambium. In the
root and stem the stem
cells of the vascular
cambium divide to
differentiate into the xylem
and phloem, the vascular
cambium then forms a ring
inside the root and shoots.
These cells divide and
grow from the ring differentiating and moving away from the cambium.
Describe and explain, with the aid of diagrams and photographs, how cells of multicellular organisms are specialised for particular
functions, with reference to erythrocytes (red blood cells), neutrophils, epithelial cells, sperm cells, palisade cells, root hair cells and
guard cells.
Neutrophills protect the body against illness, they are flexible so they can engulf
pathogens and they have lots of lysosomes with digestive enzymes that can break
down the pathogens.
Erythrocytes carry oxygen in the blood and they have a
biconcave disc shape to give a large surface area to
volume ratio for gaseous exchange, they dont have a
nucleus so they have more room for haemoglobin.
Epithelial cells cover organ surfaces and cilia can beat to
move particles, and other like microvilli can fold in the cell
membrane to increase surface area to volume ratio
Sperm cells have a flagellum that enables them to swim
to the egg and they have lots of mitochondria to provide
energy to swim, the acrosome contains digestive
enzymes so the sperm can penetrate the egg surface.
Explain the meaning of the terms tissue, organ and organ system.
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Emily Summers
A tissue is a group of similar cells that are specialized to work together to carry out a
particular function.
E.g. Ciliated epithelium, xylem tissue, squamous epithelium tissue, phloem tissue
Organs are groups of different tissues that work together to form a function.
E.g. Lungs squamous epithelium, ciliated epithelium, elastic connective tissue and
vascular tissue.
Organ systems are different organs working together for a different function, e.g. the
respiratory system is made of all of the organs, tissues and cells involved in breathing
like the lungs, trachea, larynx, nose, the diaphragm and mouth.
Discuss the importance of cooperation between cells, tissues, organs and organ systems.
Mulitcellular organisms work efficiently as they have different cells that are specialized
for various functions
It is beneficial because every different cell can carry out a specialized function in a more
efficient way than unspecialized cells could.
Each cell depends on the other cells for the functions it cannot carry out
So cells, tissues and organs in multicellular organisms cooperate to keep the organism
alive and working well.
E.g. Muscle cells can move well but to do so they need oxygen, so they need
erythrocytes to carry oxygen to them from lungs.
Module 2 Exchange and Transport
Exchange Surfaces & Breathing
Explain, in terms of surface area:volume ratio, why multicellular organisms need specialised exchange surfaces and single-celled
organisms do not.
Smaller organisms have a higher surface area to volume ratio, so single celled
organisms can diffuse substances directly in or out of the cell across the cell surface
membrane.
However, diffusion in multicellular organisms is too slow because:
13
Some cells are deep in the body, so large diffusion distance from external
environment
Emily Summers
Describe the features of the mammalian lung that adapt it to efficient gaseous exchange.
Goblet cells secrete mucus which traps pathogens and dust in the inhaled air, the cilia
on the surface of cells beat rhythmically to waft mucus at the back of the throat where
its swallowed and the stomachs acidity kills any pathogens.
Elastic fibres in the walls of the trachea, bronchi, bronchioles and alveoli aid ventilation.
They stretch and recoil to push air out when exhaling.
Smooth muscle is in the walls of the trachea, bronchi and bronchioles and it can relax to
dilate the lumen, to allow air in/out easily.
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Rings of cartilage are in the trachea walls and bronchi to give support and flexibility to
stop them collapsing on inhalation and pressure dropping.
Part of lung
Smooth
Elastic
Muscle
Fibres
Yes
Bronchi
Larger
Trachea
Cartillage
Goblet Cells
Epithelium
Yes
Yes
Ciliated
Yes
Yes
Ciliated
None
Yes
Yes
Yes
Ciliated
None
Yes
Yes
No
Ciliated
None
No
Yes
No
No cilia
None
No
Yes
No
No cilia
Large-C
shape
Bronchiole
Small
Bronchiole
Smallest
Bronchiole
Alveoli
Outline the mechanism of breathing (inspiration and expiration) in mammals, with reference to the function of the rib cage,
intercostal muscles and diaphragm
Inspiration
Intercostal and
diaphragm muscles
contract
Ribcage moves upwards
and outwards and
diaphragm flatten
increasing volume of
thorax
As the volume of the
thorax increases lung
pressure decreases
below atmospheric
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Expiration
Intercostal and
diaphragm muscles relax
Ribcage moves
downwards inwards and
diaphragm curved again
Emily Summers
pressure
Air flow into the lungs
Active process needing
energy (ATP)
pressure
Air forced out of lungs
Passive process not
requiring energy.
Explain the meanings of the terms tidal volume and vital capacity.
Describe how a spirometer can be used to measure vital capacity, tidal volume, breathing rate and oxygen uptake
1.
2.
3.
4.
A spirometer has an oxygen filled chamber with a lid that can move
The person will breathe through a tube connected to O 2 chamber
On inspiration/expiration the lid of the chamber moves up/down
The movements are recorded by a pen attached to the lid of the chamber,
and writes on a rotating drum to create a spirometer trace
5. The soda lime in the tube the person breathes into absorbs CO 2
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Emily Summers
Analyse and interpret data from a
spirometer.
Transport in animals
Explain the meaning of the terms single circulatory system and double circulatory system, with reference to the circulatory systems
of fish and mammals.
In a single circulatory system blood passes through the heart once, whereas in a double
circulatory system the blood goes through the heart twice for each complete circuit of
the body.
Explain the meaning of the terms open circulatory system and closed circulatory system, with reference to the circulatory systems of
insects and fish.
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Emily Summers
Mammals and fish have closed circulatory system, which means the blood is inside
blood vessels. The heart pumps blood into arteries which branch into capillaries, and
substances like oxygen and glucose diffuse from blood in capillaries to body cells but
blood will stay in the blood vessels, veins take blood back to the heart.
Whereas insects have open circulatory systems meaning that blood isnt contained in
blood vessels, it flows free through the body cavity.
Describe, with the aid of diagrams and photographs, the external and internal structure of the mammalian heart.
Emily Summers
Describe the cardiac cycle, with reference to the action of the valves in the heart
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SAN is a pacemaker and sends out a wave of excitation that spreads over atrial
walls
Ventricles are electrically insulated by a collagen tissue band, the right and left
atria contract
The wave of excitation spreads to the AVN from the SAN
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After a small delay of 0.1s so the atria have emptied the AVN passes the wave to
the bundle of His
The Bundle of His passes the wave to the Purkyne fibres
The Purkyne fibres carry the wave of excitation to the apex of the ventricle walls
causing them to contract simultaneously from the bottom up.
Describe the cardiac cycle, with reference to the action of the valves in the heart
20
Emily Summers
Interpret and explain electrocardiogram (ECG) traces, with reference to normal and abnormal heart activity.
21
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P= Contraction/Depolarisation of
atria
QRS= Depolarisation of ventricles
T= Repolarisation/Relaxation of
ventricles
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Emily Summers
Too fast heartbeats (i.e. 120 beats a minute) are fine during exercise, at rest however it
shows that the heart doesnt pump blood efficiently.
With the atria contracting but the ventricles not, e.g. some Ps not followed by a QRS
this could indicate a problem with the AVN, i.e. no impulse from the atria to ventricles.
Fibrillation is when the atria lose their rhythm and dont contract properly, resulting in
chest pain, fainting or even death.
Describe, with the aid of diagrams and photographs, the structures and functions of arteries, veins and capillaries.
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Emily Summers
Explain the differences between blood, tissue fluid and lymph.
Blood
Tissue Fluid
Lymph
Explanation
Erythrocytes
Yes
No
No
Leukocytes
Yes
Few
Yes
Platelets
Yes
No
No
Proteins
Yes
Few
Antibodies
Water
Yes
Yes
Yes
Dissolved
Solutes
Yes
Yes
Yes
Erythrocytes are
too large and
cannot get through
capillary walls to
tissue fluid
Most white blood
cells are in
lymphatic system
& only go to tissue
fluid on infection
In tissue fluid if
capillaries are
damaged
Too big to go
through capillary
walls
Tissue Fluid and
Lymph have a
higher water
potential than
blood
Solutes like salt
can move freely
Tissue fluid surrounds the cells and is made from substances that leave the blood, like
oxygen, water, etc. At the start of the capillary bed the pressure inside the capillaries
near the arteries is more than the pressure in tissue fluid, this difference forces fluid out
of the capillaries and into spaces surrounding cells to form tissue fluid.
When the fluid leaves the pressure is less in the capillaries, so the pressure is lower at
the end of the capillary bed nearest veins.
Because of the fluid loss the water potential at the end of the capillaries nearer the veins
is lower than the water potential in the tissue fluid, so some water will re enter the
capillaries from the tissue fluid near the veins by osmosis down a water potential
gradient.
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Emily Summers
Hb + 4O2
HbO8
Erythrocytes contain Hb, a large protein with a quaternary structure (Because it has 4
polypeptide chains)
CONFIRMATIONAL CHANGE
Each chain has a haem group containing iron, and is why Hb is red.
Hb has a high affinity for oxygen and each molecule can carry 4 O 2 molecules
It is reversible, and oxygen can dissociate from Hb near the body cells to leave Hb.
The pO2 is a measure of O2 concentration, the greater the concentration the higher the
partial pressure. So pCO2 is the measure of CO2 concentration in a cell.
Haemoglobins affinity for oxygen varies depending on the partial pressure of oxygen,
i.e. Oxygen loads onto haemoglobin to form oxyhaemoglobin when there is a high
partial pressure, and unloads at lower partial pressures.
Oxygen enters blood capillaries at the alveoli, alveoli have a high partial pressure so
oxygen will combine with Hb to form oxyhaemoglobin.
Respiring cells use oxygen and have a lower partial pressure so erythrocytes take
oxyhaemoglobin to respiring tissues and the oxygen dissociates.
Then the haemoglobin goes back to the lungs to pick up more oxygen
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Emily Summers
The fetus gets oxygen from its mothers blood across the placenta
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Emily Summers
By the time the mothers blood reaches the placenta its oxygen saturation has
decreased as some has been used by the mothers body.
For the fetus to get enough oxygen to survive its haemogobin must have a higher
affinity for oxygen than adults.
Transport in plants
Explain the need for transport systems in multicellular plants in terms of size and surface area to volume ratio.
Plants need water, CO2 minerals like nitrates and potassium, and sugars to live and
they need to get rid of waste substances. They are multicellular and have a small
surface area to volume ratio so need transport systems to move substances to and from
cells quickly as diffusion alone is too slow.
Describe, with the aid of diagrams and photographs, the distribution of xylem and phloem tissue in roots, stems and leaves of
dicotyledonous plants.
Emily Summers
Root Cross Section
Describe, with the aid of diagrams and photographs, the structure and function of xylem vessels, sieve tube elements and
companion cells.
thin layer of cytoplasm and few organelles. The cytoplasm of nearby cells is joined
through holes in sieve plates.
Companion cells are there for each sieve
tube element to carry out metabolic
processes for the sieve tube elements that
cannot survive on their own as they have
no nucleus, etc., and itself- e.g. they
provide energy for active transport of
solutes
.
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Emily Summers
A plant must open its stomata for absorption of carbon dioxide for photosynthesis,
which as a consequence allows water to escape because there is a higher water
potential inside the leaf than outside. So water moves out of the leaf by osmosis down
the water potential gradient.
Describe, with the aid of diagrams, how a potometer is used to estimate transpiration rates.
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Emily Summers
1. Cut a shoot under water to stop air from going into the xylem at a slant to
increase surface area to volume ratio for water uptake
2. Check that the apparatus has no air bubbles and is full with water
3. Put the shoot into the apparatus underwater to prevent air entering
4. Remove the photometer from the water and make it air and water tight
5. Dry the leaves, let the shoot acclimatize and shut the tap
6. Keep conditions constant throughout the experiment
7. Record the starting position of the air bubble
8. Start a stopwatch and record the distance moved by the bubble per unit time
Explain, in terms of water potential, the movement of water between plant cells, and between plant cells and their environment.
Light
Temperature
Humidity
Wind
Describe, with the aid of diagrams and photographs, how the leaves of some xerophytes are adapted to reduce water loss by
transpiration.
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Emily Summers
Describe, with the aid of diagrams, the pathway by which water is transported from the root cortex to the air surrounding the leaves,
with reference to the Casparian strip, apoplast pathway, symplast pathway, xylem and the stomata.
Water travels through the roots via the root cortex into the xylem by two ways
The Symplast Pathway
Goes through living parts of the cells, the
cytoplasm. The cytoplasm of nearby cells
connect through plasmodestmata, which are
small spaces in cell walls.
When water is in the Apoplast pathway it goes to the endodermis cells in the root, but the path is
blocked by the Casparian strip- which is just a waxy strip. The water then must take the
Symplast pathway.
This is not a hindrance because the water than has to go through the cell membrane which
controls substances entering/leaving.
If the water goes past the barrier it moves into the Xylem.
The main pathway used is the Apoplast pathway as it provides the least resistance.
Explain the mechanism by which water is transported from the root cortex to the air surrounding the leaves, with reference to
adhesion, cohesion and the transpiration stream.
Cohesion and tension move water up from roots to the leaves against gravity, water
evaporates from the leaves at the top of the xylem via transpiration
This creates suction/tension which pulls more water into the leaf
Water molecules are cohesive, meaning they stick together, so if one is pulled into the
leaf so are more. The whole column of water in the xylem moves upwards, and it enters
the stem through the roots.
Adhesion is the water molecules being attracted to the walls of the xylem vessels,
helping water rise up.
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Emily Summers
E.g. The source for sucrose is the leaves and the sinks are mainly food storage organs and the
meristems (growth areas) in the roots, stems and leaves.
Enzymes maintain the concentration from the source to the sink by changing the dissolved
substances at the sink, like by breaking them down or changing them into something else, to
make sure there is a lower concentration at the sink than the source to keep a steep
concentration gradient.
Describe, with the aid of diagrams, the mechanism of transport in phloem involving active loading at the source and removal at the
sink, and the evidence for and against this mechanism.
32
At the source active transport is said to actively load the dissolved solutes into sieve
tubes of the phloem.
Lowering the water potential inside sieve tubes and water enters them via osmosis.
Creating a high pressure inside the sieve tubes at the source end of the phloem.
At the sink the solutes are removed from the phloem to be used
Increasing water potential inside the sieve tubes so water leaves by osmosis
Lowering pressure inside the sieve tubes
This gradient is responsible for pushing solutes along the sieve tubes to where they are
required in the plant.
Emily Summers
For
Removing a ring of bark from a tree taking
the phloem not the xylem from a woody
stem a bulge will form above the ring. On
analysis of the fluid in the bulge, there will
be a higher sugar concentration above the
ring than below- so there must be a
downward sugar flow.
Aphids pierce the phloem with their
mouthparts and sap flows into them, the
sap flows out quicker nearer the leaves
than further down the stem, so there must
be a pressure gradient.
A metabolic inhibitor stopping ATP
production in the phloem stops
translocation, proving it is active transport.
There are experimental mass flow models
33
Against
Sugar travels to many sinks not one with
the highest water potential, as the model
indicates