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1. INTRODUCTION
1.1
Background : rice production in Kenya
In Kenya rice is mainly grown in Mwea, Ahero,
Bunyala, West Kano, Yala Swamp (MoA, 2011)
About 98% of Mwea rice is Basmati.
By year 2030 Kenya population is expected to
be 60.0million.
Year
2006
2030
2050
Kenya Population
38m
60m
??
Production (Tones)
64,840
??
??
Area (ha)
23,106
??
??
1.5 Objective
Use hybrid rice technology to raise yield
i) Introduce EGMS in Kenya and test for
their adaptability
ii) Develop Basmati with EGMS gene
iii) Produce hybrid by crossing EGMS-Basmati
with conventional Basmati.
2. MATERIALS
EGMS included; Photoperiod sensitive male sterile
(PGMS) rice and Thermosentive genic male sterile
lines.
PGMS Rice
i) V1 - IR-73827-23-76-15-7S
ii) V3 - IR-75589-31-27-8-33S
TGMS Rice
i) V2 - IR-77271-42-5-4-36S
Basmati370
3. METHODS
3.1 Test for EGMS adaptability
Sowing of EGMS
Growth at Sterility and assess spikelet fertility
Growth under fertility inducing conditions and
assess spikelet fertility
a
b
Fig. 1 EGMS growing in a greenhouse. Fig. a and b show
EGMS and the greenhouse respectively
a
b
c
Fig. 2 EGMS under sterility inducing conditions. Fig a shows EGMS
growing in paddy trough, Fig.b is a sterile panicle while Fig.c is pollen from
sterile panicle.
a
b
Fig. 3. EGMS grown in fertility inducing conditions . Fig. a,b&c
shows EGMS rice plant, panicles and pollen from plants grown
under fertility inducing conditions respectively with grains.
1
2
3
1
2
3
1
2
3
Rice line
Treatment
V1PGMS
V2TGMS
V3PGMS
Average
V1PGMS
V2TGMS
V3 PGMS
Average
V1PGMS
V2TGMS
V3PGMS
Average
LDL + HT
LDL + HT
LDL + HT
HT +NDL
HT +NDL
HT +NDL
NT +NDL
NT+NDL
NT+NDL
Average
Seed set per panicle %
0
3
0
1
0
2
0
0.67
45
30
26
33.67
a
b
c
Fig. 4 Hybrid rice. Fig. a show hybrid rice. Fig. a and c shows hybrid rice
plants with deep anthocyanin at the base of the plant.
Challenges
Quality traits in Basmati are under recessive
gene control
Daylength in the tropics 12hours, not long
enough to induce sterility gene particularly in
PGMS
Temperature fluctuation : may not completely
induce complete sterility.
Incompatibility of Basmati male restorer lines
with EGMS
Challenges continues
Recalcitrance in regenerating plants from F1
between EGMS and Basmati calli
Albinism in regenerants
Summary of achievements
EGMS - were very adaptable in Kenya
First hybrids have been realized
Recombinant inbreed aimed at developing
EGMS genes in Basmati background is at 3rd
forth generation
Very good response anthers to callusing
Conclusion
Production of hybrid using EGMS has been
very success in greenhouse. Thus use of hybrid
rice seeds is very promising
Acknowledgement
National Council for Science and Technology
Collaborating institutions (TUK, UoN, NIB,
KARI)
THANK YOU