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Interference
from HbC
Interference
from HbS
Interference
from HbE
Interference
from HbD
Interference
from
elevated HbF
No
No
HbA1c not
quantified
HbA1c not
quantified
No <30% HbF
Axis-Shield Afinion
No
No
No
No
Beckman AU
system
Yes
Yes
No
No
Beckman Synchron
System
No
No
No
No
No
No
No
No
No <10% HbF
Bio-Rad Variant II
NU
No
No
No <10% HbF
Bio-Rad Variant II
Turbo
No
No
Yes
Yes
No <5% HbF
Bio-Rad Variant II
Turbo 2.0
No
No
No/Yes
(conflicting
reports)
No
No <25% HbF
http://www.ngsp.org/interf.asp
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6/24/15, 1:37
Bio-Rad in2it
Yes
No
Yes
No
Ortho-Clinical Vitros
No
No
No
No
Polymer Tech
Systems A1cNOW
Yes
Yes
No
No
Roche Cobas
Integra Gen.2
No
No
No
No
Roche/Hitachi (Tina
Quant II)
No
No
No
No
Sebia Capillarys 2
Flex Piercing
No
No
No
No
No <15% HbF
No
No
Siemens
DCA 2000/Vantage
No
No
No
No
No <10% HbF
Siemens Dimension
No
No
No
No
Tosoh G7
Yes
No
Yes
No
No 30% HbF
Tosoh G8
No
No
Yes
No
No 30% HbF
Trinity (Primus)
HPLC (affinity)
No
No
No
No
No <15% HbF
@ In the absence of specific method data, it can generally be assumed that immunoassay methods do not have clinically significant interference
from HbE and HbD because the E and D substitution are distant from the N-terminus of the hemoglobin beta chain (1).
$ In the absence of specific method data, it can generally be assumed that both immunoassay and boronate affinity methods show interference
from HbF levels above ~10-15% (2,3).
More comprehensive information regarding HbA1c assay interferences
More (from the NIDDK web site):
Sickle Cell Trait and Other Hemoglobinopathies and Diabetes: Important Information for Physicians
For People of African, Mediterranean, or Southeast Asian Heritage: Important Information about Diabetes Blood Tests
References:
1. Little RR, Rohlfing CR, Hanson S, Connolly S, Higgins T, Weykamp C, D'Costa M, Luzzi V, Owen WE, Roberts WL. Effects of hemoglobin E and
D traits on glycated hemoglobin (HbA1c) Measurements by twenty-three methods. Clin Chem 2008;54:1277-82.
2. Little RR, Rohlfing CL, Hanson SE, Schmidt RL, Lin C-N, Madsen RW, and Roberts WL. The Effect of Increased Fetal Hemoglobin on 7 Common
Hb A1c Assay Methods. Clin Chem 2012 58: 945-6.
3. Rohlfing C, Connolly S, England J, Hanson S, Moellering C, Bachelder J, Little R. The effect of elevated fetal hemoglobin on HbA1c results: five
common HbA1c methods compared to the IFCC reference method. Amer J Clin Path 2008;129:811-4.
2010 NGSP
The NGSP is supported in part by National Institutes of Diabetes and Digestive and Kidney Diseases 1UC4DK096587-01.
http://www.ngsp.org/interf.asp
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