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BIOL 350: General Microbiology

FINAL EXAM
VERSION A

Beforebeginning:
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Checktomakesurethatyourtesthasall50questions.

Readeachquestioncarefully.
Thisexamisworth100points.Eachquestionisworth2points.
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1.Whichofthefollowingenergy(electron)sourceswouldaLithotrophicmicrobe
use?
A.O2
B.Iron
C.Methane
D.Alloftheabove
E.Noneoftheabove
2.WhichofthefollowingistrueofJohnSnow
A.HeuseddeadpeopletodiscoverthesourceofCholeraepidemics
B.HediscoveredtheagentthatcausedCholera
C.Withinayearofhisdiscovery,allepidemicsstoppedinLondon
D.Alloftheabovearetrue
E.Noneoftheabovearetrue
3.Theenzymenitrogenase:
A.Muststayanaerobictofixnitrogen
B.HasanFeScluster
C.UsesalotofenergytofixN2
D.Alloftheabove
E.Noneoftheabove
4.Archaea:
A.HavecirculargenomesjustlikeEukaryotes
B.Werefirstdiscoveredindeepseasediments
C.WraptheirDNAaroundhistoneproteins
D.Haveheat/pHresistantlipidbilayers
E.Noneoftheabove
5.Onethousandyeareggsareadelicacymadepossiblethrough:
A.Acidicfermentation
B.Alkalinefermentation
C.Ethanolicfermentation
D.Yeast
E.Noneoftheabove
6.Whenbreaddoughrises___
A.ThereleaseofO2causesthebreadtorise
B.PyruvateisconvertedtoethanolandCO2
C.Ethanolisremovedbytheyeast
D.Alloftheabove
E.Noneoftheabove

7.Inancientrockformations,evidenceforlifecomesintheformof:
A.Limestoneenrichedincarbon13(higherconcentrationthanregularlimestone)
B.Fossilizedfilamentousprokaryotes(microbes)

C.Oxygenbubblesintheearliestrockformations
D.Alloftheabove
E.Noneoftheabove
8.Archaealgenomes:
A.Arelinearlikeeukaroytes
B.HaveTATAbindingproteinslikeeukaryotes
C.HaveRNApolymerasesimilartobacteria
D.Encodehistoneslikebacteria
E.Alloftheabove
9.TheearliesthypotheticalcellprobablydidNOThave:
A.RNA
B.DNA
C.Lipids
D.Itprobablyhadallofthesecomponents
E.Ithadnoneofthesecomponents
10.Inwhichofthefollowingwayshavebacterialandviraldiseasesimpacted
historyandsociety?
A.Theybrokedownthesocialfabricofsociety
B.TheyfactoredheavilyincolonizationoftheAmericas
C.Theychangedthewaycitiesaredesigned
D.Alloftheabove
E.Noneoftheabove
11.Whatistrueofthenasal/oralmicroflora?
A.Thisfloraisvirtuallybacteriafreebecauseofmucous
B.Lactobacillusarecommoninthismicroflora
C.Itplaysaprotectiverolewhenenteringthebloodstream
D.NasalmicrobesneedtotoleratelowpH.
E.Noneoftheabove.

12.Whatistrueofthestomachconditionknownasachloryhydia(mainsymptom:
lossofacidity)?
A.ItallowssurvivaloforganismsliketheCholerabacterium
B.ItcreatesthehigherpHconditionsneededfortheulcerbacteriumtogrow
C.Itisassociatedwithobesity
D.Alloftheabove
E.Noneoftheabove

13.Anorganismwhichbreakdownscomplexcarboncontainingorganiccompoundsfor
energy,whichresultsinthegenerationofCO2iscalleda(an)________?4

A. heterotroph.

B.
C.
D.
E.

autotroph.
photoautotroph.
lithotroph.
prototroph.

Questions1417:Matchthefollowingorganismstotheitemsbelow.Choosethebest
answer.Onlyoneanswerperquestion,butalettermaybeusedmorethanonceor
notatall.
(A)Actinobacteria
(B)Aquificales
(C)GammaProteobacteria
(D)Spingomonas

14.Waxylipidsincellwalls
15.E.coli
16.Includefacultativelyanerobicrods
17.Showercurtains
Questions1821:Matchthefollowingorganismstotheitemsbelow.Choosethebest
answer.Onlyoneanswerperquestion,butalettermaybeusedmorethanonceor
notatall.
(A)Chlorophyta
(B)Ascomycetes
(C)Diatoms
(D)SlimeMolds
18.Chlorophyll
19.Fruitingbody
20.Diseaseknownasriceblast
21.Secondaryendosymbionts
22.TRUEofFALSE:Insoilecosystems,fungiaremajordecomposers
A.TRUE
B.FALSE
23.TRUEofFALSE:Toxingenescanundergohorizontalgenetransfer.
A.TRUE
B.FALSE
24.TRUEofFALSE:Ontheoceanfloor,theprinciplesourceofcarbonisdead
whales

a.TRUE
b.FALSE
25.TRUEofFALSE:Aerobicorganismsneedspecialcellstofixnitrogen.
a.TRUE
b.FALSE

26.WhichisthecounterstainintheGramstainprocedure?

A.
B.
C.
D.
E.

crystalviolet
methyleneblue
malachitegreen
safranin
Gramsiodine

27.ThefollowingrodshapedbacteriumwillbewhatcolorafterGramstaining?

A.
B.
C.
D.
E.

Staphylococcus,purple.
Bacillus,pink.
Streptococcus,purple.
Salmonella,purple.
Escherichiacoli,pink.

28.Whatpartofacellisreferredtoasthesacculus?

A.
B.
C.
D.
E.

nucleoid
cytoplasm
cellmembrane
cellwall
outermembrane

29.Whichnamereferstohavingflagellaattachedallaroundthecell?

A.
B.
C.
D.
E.

peritrichous
lophotrichous
monotrichous
bitrichous
flagellated

30.Analkalophilicorganismrequireswhichofthefollowingcondition(s)togrow?

A.
B.
C.
D.
E.

apHbetween911.
ahighlevelofoxygen.
highconcentrationofNaCl.
atemperatureof0oC20oC.
cangrowatbothhighandlowlevelsofoxygen.

31.Thelipopolysaccharideoftheoutermembraneisofmedicalimportancebecause
itactsasa(n)____________.

A.
B.
C.
D.
E.

specializedphospholipid.
endotoxin.
adhesin
capsulepolysaccharide.
BothBandD

32.Duringwhichstageofbacterialgrowthistheculturegrowingexponentially?

A.
B.
C.
D.
E.

lagphase
logphase
deathphase
stationaryphase
bothAandB

33.Anorganismthatrequiresinorganiccompoundsassourcesofenergy/electronsis
specificallycalleda/an______?

A.
B.
C.
D.
E.

Phototroph
Heterotroph
Lithotroph
Organotroph
Autotroph

34.Humanpathogensare

A.
B.
C.
D.
E.

halophiles.
psychrophiles.
mesophiles.
thermophiles.
extremethermophiles.

35.Whichofthefollowingisadormantformofbacteriaresistanttodesiccation
andothernaturalstresses?

A.
B.
C.
D.
E.

ribosome
carboxysomes
storagegranules
thylakoids
endospore

36.WhichofthefollowingmutagensmaycauseThyminedimers?

A.
B.
C.
D.
E.

Xrays
5bromouracil
nitrosoguanidine
UVradiation
ethidiumbromide

37.Whichofthefollowingformsoftransmissionspecificallyrequirescelltocell
contactforDNAtransfertooccur?

A.
B.
C.
D.
E.

specializedtransduction
replicativetransposition
transformation
generaltransduction
conjugation

38.Whichofthefollowingformsoftransmissionspecificallyrequires
bacteriophage?

A.
B.
C.
D.
E.

transduction
replicativetransposition
transformation
generalrecombination
conjugation

39.WhichmoleculeisresponsiblefortheinitialopeningoftheDNAhelixduring
DNAreplication?

A.
B.
C.
D.
E.

DNAprimase
singlestrandedbindingprotein
DNApolymeraseIII
DnaA
DnaB

40.Whenabacterialcultureisgrowing,underwhichofthefollowingconditions
willCatabolightRepressionoccur?

A.
B.
C.
D.
E.

Glucosepresent,Lactosepresent
OnlyLactosepresent
Glucoseabsent,Lactosepresent
Glucoseabsent,Lactoseabsent
BandConly

41.Whichprocessofthebacterialcellservesasanidealtargetforantibiotic
therapy?

A.
B.
C.
D.
E.

cellwallsynthesis
DNAreplication
proteinsynthesis
Transcription
alloftheabove

42.Anantibioticwhichkillsthebacteriumisconsideredtobewhat?

A.
B.
C.
D.
E.

bactericidal
broadspectrum
narrowspectrum
bacteriostatic
alloftheabove

43.Whichbacteriumcausesmeningitisprimarilyinnewbornsbeingpassedfromthe
mothertotheinfant?

A.
B.
C.
D.
E.

Streptococcuspneumoniae
Hemophilusinfluenzae
Neisseriameningitidis
GroupBstreptococcus
Bacillusanthracis

44.Betalactamasesworkbywhichmechanismtoachieveantibioticresistance?

A.
B.
C.
D.
E.

additionofmodifyinggroupsthatinactivatetheantibiotic
effluxpumps
destructionoftheantibiotic
modificationofcelltargetprotein
alloftheabove

45.Penicillininhibitswhatcomponentofcellwallsynthesis?

A.
B.
C.
D.
E.

autolysins.
transpeptidases.
transglycosidases.
bactoprenols.
Nacetylglucosamines.

46.Whichpartofthecellularinnatedefensesystemisknownasthebigeater
responsibleforkillingmicrobes?

A.
B.
C.
D.

neutrophil.
naturalkillercell.
Bcells.
macrophage.

E.dendriticcell.

47.WhichisthelargestPhysicalbarriertoinfection?

A.
B.
C.
D.

mucous.
skin.
ciliaescalator.
tolllikereceptors.

E.vomitinganddiarrhea.

48.Whichofthefollowingbacteriumisassociatedwithgastrointestinaltract
infections,andspecificallystomachulcers?

A.
B.
C.
D.
E.

Campylobacterjejuni
Bacillusanthracis
Staphylococcusaureus
Helicobacterpylori
EnteroinvasiveE.coli

49.Whichofthefollowingbacteriumisconsideredazoonoticpathogen?

A.
B.
C.
D.
E.

Campylobacterjejuni
Bacillusanthracis
Staphylococcusaureus
Helicobacterpylori
EnteroinvasiveE.coli

50.Whichofthefollowingbacteriumhasbeenusedasabioweapon?

A.
B.
C.
D.
E.

Campylobacterjejuni
Bacillusanthracis
Staphylococcusaureus
Helicobacterpylori
EnteroinvasiveE.coli

BIOL 350 FINAL EXAM Fall 10