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ABSTRACT
RESUMEN
Water supply is the main limiting factor that affects oil palm
(Elaeis guineensis Jacq.) yield. This study aimed to evaluate
the gas exchange and photosynthetic capacity, determine the
physiological effects and assess the tolerance potential of oil
palm genotypes under water-deficit conditions. The two oil
palm commercial genotypes IRHO1001 and IRHO7010 were
exposed to soil water potentials of -0.042 MPa (field capacity or
well-watered) or -1.5 MPa (drought-stressed). The leaf water potential and gas exchange parameters, including photosynthesis,
stomatal conductance, transpiration and water use efficiency
(WUE), as well as the photosynthesis reduction rate were monitored at 4 and 8 weeks after treatment. The IRHO7010 genotype
showed fewer photosynthesis changes and a smaller photosynthetic reduction under the prolonged water deficit conditions
of 23% at 4 weeks after the treatment as compared to 53% at
8 weeks after treatment, but the IRHO1001 genotype showed
46% and 74% reduction at the two sampling times. IRHO7010
had a higher stomatal conductance and transpiration potential
than IRHO1001 during the water shortage. The WUE and
leaf water potential were not different between the genotypes
during dehydration. The data suggested that IRHO7010 had
a higher photosynthetic capacity during the drought stress and
was more drought-tolerant than IRHO1001.
Introduction
During plant evolution, physiological regulators, such as
stomatal and guard cell adjustment, chloroplast reactions,
membrane depolarization and expression-to-function
signaling, have been developed to save water and optimize
water use for subsequent periods (Wasilewska et al., 2008;
Sirichandra et al., 2009). These mechanisms enable plants
Received for publication: 26 March, 2015. Accepted for publication: 30 June, 2015.
Jazayeri, Rivera, Camperos-Reyes, and Romero: Physiological effects of water deficits on two oil palm (Elaeis guineensis Jacq.) genotypes
165
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166
soil for the oil palm in Colombia. The water potential of the FC and WP
was calculated with both the volumetric and weight-based water content using Eq. 1 and a soil density of 1.2 g cm-3.
Point
Percentage
(w/w)
Percentage volumetric
water content
Water potential
(MPa)
33.08
19.48
39.70
23.38
- 0.042
- 2.4
Estimated FC
Estimated WP
Humidity (%)
y = 26.19x-0.131
R = 0.9921
30
20
10
0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
IRHO7010
0 WAT
0
WW
4 WAT
DT
WW
a a
a a
8 WAT
DT
WW
DT
-0.5
-1.0
-1.5
a a
a a
-2.0
b b
b b
-2.5
Field capacity
40
IRHO1001
60
50
There were no differences in the leaf water potentials between the sampling times, i.e. 4 and 8 WAT, in the genotypes (Fig. 2). The leaf water potential for the WW plants
of both genotypes was approximately constant and did not
show any significant difference during the sampling times,
0, 4 and 8 WAT (Fig. 2).
Results
1.6
Figure 1. Soil water retention curve for the oil palm in Colombia. Field
capacity of the soil was around 50% humidity. The WP was calculated
as -2.4 MPa to determine the drought-treatment water potential in the
soil. -1.5 MPa was selected as a severe drought point for the oil palms
before reaching the WP.
Jazayeri, Rivera, Camperos-Reyes, and Romero: Physiological effects of water deficits on two oil palm (Elaeis guineensis Jacq.) genotypes
167
DT_ IRHO7010
WW_IRHO7010
DT_ IRHO1001
WW_IRHO1001
0.45
14
12
10
0.40
gs (mol H2O m-2 s-1)
DT_ IRHO7010
WW_IRHO7010
DT_ IRHO1001
WW_IRHO1001
0.35
0.30
0.25
0.20
0.15
0.10
0.05
2
0
WAT
WAT
IRHO7010 and IRHO1001, subjected to drought in Colombia. WW, wellwatered (field capacity -0.042 MPa); DT, drought treatment (-1.50 MPa);
WAT, weeks after beginning of the treatment. Results are meanSD
(n=12). Measurements were taken at 4 and 8 weeks after exposure to
the treatments.
DT_ IRHO1001
WW_IRHO1001
DT_ IRHO7010
WW_IRHO7010
6
E (mmol H2O m-2 s-1)
5
4
3
2
1
0
WAT
DT_ IRHO7010
WW_IRHO7010
DT_ IRHO1001
WW_IRHO1001
WW_IRHO7010
0.982
DT_IRHO1001
3.5
2.0
WW_IRHO1001
1.5
1.0
0.5
0
3.535
-2.21
2.5
DT_IRHO7010
PC2 (14.9%)
3.0
-1.78
0
PC1 (79.4%)
WAT
Figure 6. Water use efficiency (WUE) of two oil palm genotypes, i.e.
IRHO7010 and IRHO1001, subjected to drought in Colombia. WW, wellwatered (field capacity -0.042 MPa); DT, drought treatment (-1.50 MPa);
WAT, weeks after beginning of the treatment. Results are meanSD
(n=12). Measurements were taken at 4 and 8 weeks after exposure to
the treatments.
Discussion
A water potential less than -1.5 MPa has been considered
as severe drought condition for many plants because it
can affect physiological variables harshly, decreasing
photosynthesis and stomatal conductance, changing some
metabolites and regulating gene expression to mitigate
water deficit effects (Vsquez-Robinet et al., 2010; Liu et al.,
2011; Kakumanu et al., 2012). In the current study, the soil
Jazayeri, Rivera, Camperos-Reyes, and Romero: Physiological effects of water deficits on two oil palm (Elaeis guineensis Jacq.) genotypes
169
The WUE can reveal how a plant is able to fix carbon during drought conditions based on the water used (Bacon,
2004), but it varies because of different factors, such as leaf
spatial orientation, seasonal effects, nocturnal and diurnal
molecular responses, and single leaf vs. whole plant study
scale, etc. (Medrano et al., 2015). Because of its variation,
the WUE cannot be used solely to determine drought
tolerance as other variables can, i.e. photosynthesis and
transpiration, but it may be used to complete the results
of photosynthesis and transpiration studies during water
shortages.
As shown in Fig. 7, the samples were successfully categorized by Multibase2015 using a PCA analysis according to
their physiological responses into four different groups,
including two genotypes by two water treatments separately. The WW plants were completely different from the
DT plants in both genotypes and the DT plants were totally
distinct between both genotypes. These findings suggest
that the two genotypes behaved differently in terms of
the studied physiological parameters and were successfully classified based on their drought-tolerance levels,
i.e. IRHO7010 as tolerant and IRHO1001 as susceptible.
Conclusion
This study examined two oil palm genotypes for their
drought tolerance by evaluating physiological variables, including photosynthesis, stomatal conductance,
Agron. Colomb. 33(2) 2015
transpiration, water use efficiency and leaf water potential. The photosynthetic rate was used to differentiate the
genotypes. This characteristic was ascribed to the ability of
IRHO7010 to control water in the leaves. Higher stomatal
conductance and transpiration were observed during the
prolonged water shortage in IRHO7010. The photosynthesis reduction rate showed a slower diminishing rate in
the IRHO7010 plants than those of IRHO1001. Thus,
our results suggest that IRHO7010 was able to mitigate
the prolonged drought conditions more effectively than
IRHO1001 during the initial phase of development.
IRHO7010 is considered as more drought-tolerant than
IRHO1001, according to the obtained results.
These results can be used in downstream studies on these
oil palm genotypes in omics studies, such as genomics and
transcriptomics to find likely genes, pathways, processes
and mechanisms which are involved in oil palm drought
tolerance and in terms of oil palm plant breeding programs
based on molecular responses.
The strategy of studying changes of desired physiological
parameters between WW and DT plants (i.e. reduction
percent of photosynthesis, transpiration and stomatal
conductance) as a whole package seems more efficient for
deducing tolerance levels in different genotypes, rather
than studying each parameter individually and separately.
IRHO7010, as the more tolerant genotype, can be recommended for cultivation in Colombian oil palm plantations,
where water deficits are a regional problem. Of course, the
production and yield potential of both genotypes should
be taken into account in further studies to more effectively
support this suggestion.
Acknowledgments
Research at Cenipalma is funded by the Colombian Oil
Palm Promotion Fund (FFP), administered by Fedepalma
(Federacin Nacional de Cultivadores de Palma de Aceite).
The authors would like to thank Mr. Cristihian Jarri Bayona
Rodriguez for his scientific support, Mr. Wilson Diaz Castillo for his field assistance at Cenipalma and Mrs. Eloina
Mesa Fuquen for her assistance with the statistical analyses.
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