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CHA University
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Keywords: carboxymethyl cellulose, polyvinylpyrrolidone, multicomponent semi-IPN polymer hydrogel bead, pH-responsive,
controlled release
References
[1] M. Shen, Y.Z. Huang, L.M. Han, J. Qin, X.L. Fang, J.X. Wang, V.C.
Yang, Multifunctional drug delivery system for targeting tumor
and its acidic microenvironment, J. Control. Release 161 (2012)
884892.
[2] W.B. Wang, Q. Wang, A.Q. Wang, pH-Responsive carboxymethylcellulose-g-poly(sodium acrylate) /polyvinylpyrrolidone
semi-IPN hydrogels with enhanced responsive and swelling
properties, Macromol. Res. 19 (2011) 5765.
doi:10.1016/j.jconrel.2015.05.152
PEG shell are one of the versatile carriers due to their complementary characteristics of both polymeric nanoparticles and liposomes
[2]. In this study, we fabricated protamine incorporated cationic
DOTAP lipid layered PLGA hybrid nanoparticles (LPHNPs) as a gene
delivery system by convenient double emulsion solvent evaporation
method and examined the effect of lipid concentration on the
properties of LPHNPs (i.e. size). DLS results conrmed relatively
monodisperse LPHNPs with a Z-average size of 153 nm and low
polydispersity (PDI-0.07) and TEM result conrmed the structure of
a hydrophobic PLGA core and cationic lipid shell (Fig. 1). The zeta
potential shifted from a negative value ( 24 mV) to a positive value
(+60) with the incorporation of cationic lipid on the surface of
PLGA, which was probably induced by the hydrophobic interaction
between lipids and PLGA. We also found that the size and surface
charge of LPHNPs increased with the increase of cationic lipid
concentration indicating easy modulation of the surface properties of
LPHNPs. Long term stability of those complexes was also observed.
Additionally, transfection and viability of HEK 293 cells treated with
LPHNPs/DNA complexes with different concentrations were evaluated by ow cytometry. In all concentrations, LPHNPs/DNA complexes
exhibited strong transfection efciency while maintaining high cell
viability, suggesting LPHNPs as an excellent and versatile gene
delivery system.
Fig. 1. TEM images of lipid polymer nanoparticle and size graph with undersize curve
(upper), the zeta potential of bare PLGA and LPHNPs (scale bar50 nm).
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doi:10.1016/j.jconrel.2015.05.154
Acknowledgements
This work was supported by the Fusion Research Program for Green
Technologies through the National Research Foundation of Korea