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A. They lower the reaction energy by stabilizing the [ES]* transition state.
B. They increase substrate concentration to more effectively bind substrate to
product.
C. They introduce energy from ATP into the reaction.
D. They remove the product so that the reaction is not product-inhibited.
E. They deactivate inhibitors to the reaction.
2)
A.
B.
C.
D.
E.
3) During the enzyme kinetics lab, you used a _____________ to directly monitor
the progress of the reaction.
A. balance
B. spectrophotometer
C. stopwatch
D. microscope
E. balance with a stopwatch
4)
A.
B.
C.
D.
E.
5) What is a PCR
A. Polyunsaturase Chain reaction
B. Polysaturase Chain reaction
C. Polymerase Chain Reaction
D. Pectinase Chain Reaction
E. None of the Above
6) What step of PCR is where primers go and attach to DNA strand
A. Annealing
B. Extension
C. Denaturation
D. Heating
E. Cooling
7) How many hydrogen bonds are present between A-T base pair
A.
B.
C.
D.
E.
One
Two
Three
Four
None of the above
A.
B.
C.
D.
E.
340nm
380nm
590nm
560nm
None of the above
Tube
Blank
1
2
3
4
5
[Pyruvate]
(mM)
0.0
0.2
0.4
0.6
0.8
1.0
[NADH]/min
(mM/min)
0.00
0.06
0.13
0.18
0.21
0.22
2) Using the following data, plot a Michaelis-Menten curve in the graph area below.
Label the x- and y-axes correctly, and indicate the approximate Km and Vmax
values on the plot. Absorption co-efficient of NADH at 340nm is 6220 M-1cm-1
(10points)
Tube
Enzyme(L
)
Blank
1
2
3
4
5
50
50
50
50
50
50
3mM
NADH(L
)
100
100
100
100
100
100
14mM
Buffer(L)
pyruvate(L)
0
20
40
80
160
320
850
830
810
770
690
530
Substrate
Concn
(mM)
V0 (A340 V0
(mM
-1
min )
NADH ml-1
min-1)
0
0.06
0.13
0.18
0.21
0.22
3) AFFINITY CHROMOTOGRAPHY
True/False: Dehydrogenases are broadly known as oxidoreductases because they transfer
electrons from one molecule to another.(2points)
Why did we use Cibacron Blue in the column? Explain briefly how this aided in isolating
LDH. (4 points)
What technique did you use to identify the rate at which NAD+ was converted to NADH
and what did that tell you about your fractions?(4 points)
4) Write in short sentences about following terms
a.
b.
c.
d.
e.
f.
g.
h.
i.
Affinity chromatography
Two factors of Enzyme kinetics.
GMO
Gel electrophoresis
PCR
DNA
Promoter of a gene
LDH
Molisch Test