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Name: _________________
Answer all of the following questions and record your answer on the answer sheet.
You must show all of your calculations in order for any credit to be given. You must
box your final answers on any scratch paper that you include with this Problem Set.
If I cant follow your work, you wont receive partial credit.
1. Km = _________________
Vm = _________________
1.
2.
3.
V (nmol/min)
___________
0.20
0.26
0.33
1.0
1.43
1.67
2.08
3.33
V (mmol/min)
___________
10
[S]1
[S]2
[S]3
[S]4
1.2
1.7
2.1
2.2
2.5
The effect of an inhibitor on an enzyme was tested and the experiment gave
the results below. Plot the data and determine, by inspection of the graph,
what type of inhibition is involved.
[S] M
______
0.4
0.67
1.00
2.00
4.
V (mol/min)
with 0.0 nM
Inhibitor
___________
V (mol/min)
with 25 nM
Inhibitor
___________
V (mol/min)
with 50 nM
Inhibitor
___________
0.22
0.29
0.32
0.40
0.21
0.26
0.30
0.36
0.20
0.24
0.28
0.32
5.
The following questions refer to the figure. You must CALCULATE every
answer, you cannot simply look at the titration curve and make an
approximatation.
i)
ii)
iii)
6.
The following questions refer to the figure. You must CALCULATE every
answer, you cannot simply look at the titration curve and make an
approximatation.
i)
ii)
iii)
ii)
2,4-dimethyl-3-pentanol
1-ethyl-3-methylbenzene
2,6-diaminohexanoic acid
1-methoxy-3-hexanone
benzaldehyde