Diaclone Immunoassay Catalogue

2004-2005

Cytokines •
Cytokines receptors •
Adhesion molecules •
Apoptosis •
CD antigens •
 IMMUNOASSAY CATALOGUE

TABLE OF CONTENTS

EDITORIAL .........................................................................................................................................................................................3

ELISA ..............................................................................................................................................................................................4-14
Human 6-12
Apoptosis 6
Cytokines / Growth Factors 6-9
Cytokines Receptors 10
Adhesion molecules 11
Soluble CD Antigens 12

Mouse 13
Cytokines
14
Rat
Cytokines

ELI-PAIRS ....................................................................................................................................................................................15-16
Human 16
Mouse 16
Rat 16

ELISPOTS ....................................................................................................................................................................................17-22
Elispot - Enzymatic 18
Human 19-20
Mouse 20
Rat 20-21

Human Fluorospot 21-22

Human Dual Elispot 22

ELISPOT MATCHED ANTIBODY PAIRS ......................................................................................................................................23

Human 23
Mouse 23
Rat 23

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 EDITORIAL

Another
new Diaclone ratories also keeps us close to the latest develop-
catalogue is ments and their needs for new products.
lying in front I hope we can continue to give you full satisfac-
of you and when thinking about the history of tion now and in the future.
Diaclone Research products I realise that we have
been on the market for over ten years. The first
catalogue contained antibodies and 6 Elisa kits
filling 18 pages of product information in total!
Even by writing smaller and smaller (sorry for the
spectacles) and skipping unessential information
we now need two catalogues to present our pro-
duct range showing the important increase of our
product development since the beginning. This is
not only related to new products but also to new
formats like the high sensitive ELISAs, CE marked
ELISAs, Fluorospots and CE labelled CD138. We
have noticed that creating new formats with higher
levels of quality control is actually increasing the
overall quality of all products. This is also reflec- Besançon, March 2004
ted in fewer customer complaints, a criterion Dr. John Widjenes,
important for the quality of the products.
Another change leading to increased quality is
the separation made between the therapeutic and
research / diagnostic activity. Being a company
fully dedicated to the development, production
and sales of the diagnostic and research products
has also increased performances and is visible,
for instance, by the recently developed new pac-
kaging for the monoclonal antibody vials. CEO of Diaclone
Being present in more than 30 countries is com-
forting for our self-esteem. Being appreciated by
one’s home country is one thing; being appre-
ciated in so many other countries is not easily
obtained. Sending a product 100 kilometres away
does not entail the same logistics as sending a
product 10,000 kilometres away. Even so, we try
to satisfy all our customers to the same extent, be
they close or far.
This new structure generates a sense of total
coordination; a well-oiled organisation which
manages to integrate all aspects of our work,
from development and production, to marketing,
sales and shipment.
Our continuous contact with many research labo-

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 ELISA

Diaclone is proud to introduce 4 CE-marked Elisa Kits : IFNγ, IL-6, IL-10 and TNFα. These CE-marked reagents meet the requirements
of the 98/79/EC directive and provide you with an addition guarantee. We will continually introduce new CE-marked Elisa kits to fullfill
your needs.

Diaclone Elisa Kits are ready-to-use. With pre-coated 12 strip plates, standards, buffers, detection antibody, conjugate and substrate,
you can easily and with high sensitivity measure molecule secretion in serum, plasma or cell culture supernatants.

Advantages of Diaclone Elisa

Wide choice
Large range of antigens: cytokines, cytokine receptors, adhesion molecules, apoptosis related proteins, CD antigens.
Several species: human, mouse and rat

High performance
Fast procedure: average incubation time for human kits 1 1/2 hours
High sensitivity
Both natural and recombinant antigen specificity
High reproductibility
Standard calibration to NIBSC/WHO (when available)

CE-marked Elisa
CE-marked Elisa kits available for IFNγ, IL-6, IL-10 and TNFα.

Iso quality
Accuracy and reliability guaranteed: all of our reagents have been extensively validated according to the ISO 9001 quality system

Convenience
12 strip plates: which enable to perform two assays per plate
Two types of standard dilution buffers: (when necessary) to be as close as possible to the sample composition
Demo kit: available upon special request
Several sizes: available in 1 or 2 x 96 well-plate formats
Similar procedure for all kits

Elisa kit contents

Pre-coated 12 strip plates
Biotinylated secondary antibody
Standards
Controls (when available)
Buffers
Streptavidin-HRP
TMB
Stop reagent (H2SO4)
Detailed procedure datasheet

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 PROCEDURE SUMMARY

All incubations are performed

at room temperature.

Plate

Pre-coated plate ready-to-use

Dilute antigens

Note :
Standard, control or sample
In a few cases (not shown) antigen
and biotinylated antibody are added in appropriate diluent buffers
step by step.

and add to wells

+
Dilute biotinylated antibody
in appropriate diluent
Wash and add to wells

Dilute streptavidin-HRP
in its diluent
and add to wells

Wash

Add TMB substrate, incubate

in the dark, stop the reaction
by adding H2SO4

READ AT 450 nm

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Human Apoptosis
1x96 tests 850.740.096
2x96 tests 850.740.192
•Range :...................... 93.75 pg / ml - 3000 pg / ml
•Sensitivity :................ 47 pg / ml
•Incubation :................ 1 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
Reference:
Girard M. et al., Int. J. Parasitol., 2003; 33 : 713 - 720
1x96 tests 850.790.096
2x96 tests 850.790.192
•Range :...................... 31.25 pg / ml - 1000 pg / ml
•Sensitivity :................ 20 pg / ml
•Incubation :................ 3 h
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
1x96 tests 850.770.096
2x96 tests 850.770.192
•Range :...................... 93.75 pg / ml - 3000 pg / ml
•Sensitivity :................ 64 pg / ml
•Incubation :................ 3 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
References:
Wandinger KP et al., Lancet, 2003; 14 : 2036 - 2043
Lünemann JD. et al., J. Immunol., 2002; 168 : 4881 - 4888
Human Cytokines / Growth Factors
1x96 tests 650.050.096
GM-CSF
•Range :...................... 8 pg / ml – 500 pg / ml
•Sensitivity :................ 4.4 pg / ml
•Incubation :................ 2 h 15
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 50 µl
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ELISA
1x96 tests 850.750.096
CD95 / Fas CD178/Fas L 2x96 tests 850.750.192
•Range :...................... 62.5 pg / ml - 2000 pg / ml
•Sensitivity :................ 44 pg / ml
•Incubation :................ 3 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
References:
Girard M. et al., Int. J. Parasitol., 2003; 33 : 713 - 720
Kern P. et al., Infection and Immunity 2000; 68(5) : 3061 - 3063
1x96 tests 850.630.096
Granzyme B p53 2x96 tests 850.630.192
•Range :...................... 3.1 U / ml - 100 U / ml
•Sensitivity :................ 1.5 U / ml
•Incubation :................ 3 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
Trail / APO2L
1x96 tests 950.000.096 CE
IFNγ 2x96 tests 950.000.192 CE
•Range :...................... 12.5 pg / ml – 400 pg / ml
•Sensitivity :................ 5 pg / ml
•Incubation :................ 2 h 40
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
•CE-Marked
References:
Dembinski J. et al., Cytokine, 2003; 21 : 200 - 206
Pilch H. et al., Clin. Diagn. Lab. Immunol., 2002; 9 : 267 - 278
Saverino D. et al., J. immunol., 2002; 168 : 207 - 215
Fahy O. et al., J. immunol., 2002; 168 : 5912 - 5919
Hammad H. et al., Blood, 2001; 98 : 1135 - 1141
Carcelain G. et al., J. Virol., 2001; 75(1) : 234 - 241
Zarkesh-Esfahani S. H. et al., J. Clin. Endocrinol. Metab., 2000; 85(9) : 3383 - 3390
Woerly G. et al., J. Exp. Med., 1999; 190(4) : 487 -496
Tayebi H. et al., J. Immunol. Methods., 1999; 229 : 121 - 130
Hasegawa D. et al., Blood, 1998; 91: 2793 - 2799
65

Human Cytokines / Growth Factors
1x96 tests 850.005.096
2x96 tests 850.005.192
•Range :...................... 31.2 pg / ml - 1000 pg / ml
•Sensitivity :................ 10 pg / ml
•Incubation :................ 3 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
1x96 tests 850.010.096
2x96t ests 850.010.192
•Range :...................... 31.2 pg / ml - 1000 pg / ml
•Sensitivity :................ 10 pg / ml
•Incubation :................ 1 h 50
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
References:
Blanco B. et al., J. Immunol., 2003 ; 170 : 5464 - 5469
Saverino D. et al., J. immunol., 2002; 168 : 207 - 215
Kapsogeorgou E.K. et al., J. Immunol., 2001; 166 : 3107 - 3113
Woerly G. et al., J. Exp. Med., 1999; 190(4) : 487 -496
1x96 tests 850.025.096
2x96 tests 850.025.192
•Range :...................... 7.8 pg / ml – 250 pg / ml
•Sensitivity :................ 5 pg / ml
•Incubation :................ 3 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
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ELISA
1x96 tests 850.006.096
IL-1α IL-1β 2x96 tests 850.006.192
•Range :...................... 15.6 pg / ml - 500 pg / ml
•Sensitivity :................ 5 pg / ml
•Incubation :................ 3 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
Reference:
Young Kwon K. et al., J. Korean Med. Sci., 2001; 16 : 774 - 780
1x96 tests 850.020.096
IL-2 IL-4 2x96 tests 850.020.192
•Range :...................... 1.1 pg / ml – 35 pg / ml
•Sensitivity :................ 0.5 pg / ml
•Incubation :................ 3 h 50
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
References:
Fahy O. et al., J. immunol., 2002; 168 : 5912 - 5919
Hammad H. et al., Blood, 2001; 98 : 1135 - 1141
1x96 tests 850.035.096
IL-5 IL-6 High Sensitivity 2x96 tests 850.035.192
•Range :...................... 1.56 pg / ml – 50 pg / ml
•Sensitivity :................ 0.8 pg / ml
•Incubation :................ 3 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
1x96 tests 950.030.096 CE
IL-6 2x96 tests 950.030.192 CE
•Range :...................... 6.25 pg / ml – 200 pg / ml
•Sensitivity :................ 2 pg / ml
•Incubation :................ 1 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
• CE Marked
References:
Balabanian K. et al., Am. J. Respir. Crit. Care Med., 2002; 165 : 1419 - 1425
Young Kwon K. et al., J. Korean Med. Sci., 2001; 16 : 774 - 780
Kube D. et al., Blood, 2001; 98 : 762 - 770
Kulms D. et al., J. Biol. Chem., 2000; 275 : 15060 - 15066
Corbi P. et al., Eur J Cardiothorac Surg., 2000; 18 : 98 - 103
Le Meur Y. et al., Nephrol. Dial. Transplant., 1999; 14(10) : 2420 - 2426
Vita N. et al., J. Immunol., 1997; 158 : 3457 - 3462
7

Human Cytokines / Growth Factors
1x96 tests 850.040.096
2x96 tests 850.040.192 IL-7
•Range :...................... 6.25 pg / ml - 200 pg / ml
•Sensitivity :................ 3 pg / ml
•Incubation :................ 2 h 40
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
Reference:
Triantafilou K. et al., J. Cell Sci., 2001; 114 : 2535 - 2545
1x96 tests 950.060.096 CE
2x96 tests 950.060.192 CE IL-10
•Range :...................... 12.5 pg / ml - 400 pg / ml
•Sensitivity :................ 5 pg / ml
•Incubation :................ 2 h 40
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
•CE Marked
References:
Perez-Bercoff D. et al., J. Virol., 2003; 77 : 4081 - 4094
Perez S A. et al., Blood, 2003; 101 : 3444 - 3450
Gosset P. et al., J. Immunol., 2003 ; 170 - 4943 - 4952
Schneider E M. et al., Blood, 2002; 100 : 2891 - 2898
Gary-Gouy H. et al., Blood, 2002; 100 : 4537 - 4543
Saverino D. et al., J. immunol., 2002; 168 : 207 - 215
Sammons J. et al., Stem Cells 2000; 18 : 214 - 219
Oksenhendler E. et al., Blood, 2000; 96 : 2069 - 2073
Woerly G. et al., J. Exp. Med., 1999; 190(4) : 487 -496
Abbal C. et al., Int. Immunol., 1999; 11(9) : 1451 - 1462
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ELISA
1x96 tests 850.050.096
IL-8 2x96 tests 850.050.192
•Range :...................... 62.5 pg / ml - 2000 pg / ml
•Sensitivity :................ 25 pg / ml
•Incubation :................ 1 h 40
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
References:
Bäcked F. et al., J. Biol. Chem., 2002; 277 : 18198 - 19205
Ellmerich S. et al., Carcinogenesis, 2000; 21 : 753 -756
1x96 tests 850.070.096
IL-12p70 2x96 tests 850.070.192
•Range :...................... 6.25 pg / ml – 200 pg / ml
•Sensitivity :................ 3 pg / ml
•Incubation :................ 3 h 40
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
References:
Gosset P. et al., J. Immunol., 2003 ; 170 - 4943 - 4952
Rea D. et al., J. Immunol., 2001; 166 : 5236 -5244
Rea D. et al., Blood, 2000; 95 : 3162 - 3167
Verdijk R M. et al., J. Immunol., 1999; 163 : 57 - 61
Rea D. et al., J. Virol., 1999; 73 : 10245 -10253
1x96 tests 850.810.096
IL-12 p70 High Sensivity 2x96 tests 850.810.192
•Range :...................... 0.78 pg / ml – 25 pg / ml
•Sensitivity :................ 0.75 pg / ml
•Incubation :................ 4 h 00
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
8
 ELISA
Human Cytokines / Growth Factors
1x96 tests 850.075.096 1x96 tests 850.080.096
2x96 tests 850.075.192 IL-12p40+p70 IL-13 2x96 tests 850.080.192
•Range :...................... 62.5 pg / ml – 2000 pg / ml •Range :...................... 3.12 pg / ml – 100 pg / ml
•Sensitivity :................ 20 pg / ml •Sensitivity :................ 1.5 pg / ml
•Incubation :................ 1 h 45 •Incubation :................ 2 h 10
•Samples :................... Serum, Plasma, Cell culture supernatant •Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl •Sample size :............. 100 µl
References:
Saverino D. et al., J. immunol., 2002; 168 : 207 - 215
Ota M. O. C. et al., J. Immunol., 2002; 168 : 919 - 925
Fahy O. et al., J. immunol., 2002; 168 : 5912 - 5919
Abbal C. et al., Int. Immunol., 1999; 11(9) : 1451 - 1462

1x96 tests 650.010.096 1x96 tests 650.020.096

TGF-β1
•Range :...................... 0.5 ng / ml - 30 ng / ml
•Sensitivity :................ 1.36 pg / ml
•Incubation :................ 5 h
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
TGF-β2
•Range :...................... 31.25 pg / ml - 1000 pg / ml
•Sensitivity :................ 10 pg / ml
•Incubation :................ 5 h 30
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl

1x96 tests 950.090.096 CE

TNF-α 2x96 tests 950.090.192 CE
•Range :...................... 25 pg / ml – 800 pg / ml
•Sensitivity :................ 8 pg / ml
•Incubation :................ 3 h 40
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
•CE Marked
References:
Perez S A. et al., Blood, 2003; 101 : 3444 - 3450
Niemand C. et al., J. Immunol., 2003; 170 : 3263 - 3272
Latz E. et al., J. Biol. Chem., 2002; 277 : 47834 - 47843
Young Kwon K. et al., J. Korean Med. Sci., 2001; 16 : 774 - 780
Kothny-Wilkes G. et al., J. Biol. Chem., 1999; 274 : 28916 - 28921
Le Meur Y. et al., Nephrol. Dial. Transplant., 1999; 14(10) : 2420 - 2426

1x96 tests 650.040.096 1x96 tests 650.030.096

VEGF-A
•Range :...................... 16 pg / ml - 1000 pg / ml
•Sensitivity :................ 11 pg / ml
•Incubation :................ 2 h 25
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 25 µl
VEGF-R1
•Range :...................... 0.16 pg / ml - 10 ng / ml
•Sensitivity :................ 0.1 ng / ml
•Incubation :................ 3 h 30
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 50 µl

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 ELISA
Human Cytokine Receptors
1x96 tests 850.500.096 1x96 tests 850.660.096
2x96 tests 850.500.192 CD25/IL-2R CD116/GM-CSF R 2x96 tests 850.660.192
•Range :...................... 68.75 pg / ml – 2220 pg / ml •Range :...................... 31.25 pg / ml – 1000 pg / ml
•Sensitivity :................ 8 pg / ml •Sensitivity :................ 16 pg / ml
•Incubation :................ 3 h 40 •Incubation :................ 3 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant •Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl •Sample size :............. 100 µl
Reference: Reference:
Foussat A. et al., Blood, 2001; 98 : 1678 - 1686 Prevost JM. Et al., J. Immunol., 2002; 169 : 5679 - 5688

1x96 tests 850.700.096 1x96 tests 850.670.096

2x96 tests 850.700.192 CD117/c-KIT CD124/IL-4R 2x96 tests 850.670.192
•Range :...................... 0.312 ng / ml – 10 ng / ml •Range :...................... 31.25 pg / ml - 1000 pg / ml
•Sensitivity :................ 0.095 ng / ml •Sensitivity :................ 11.5 pg / ml
•Incubation :................ 1 h 45 •Incubation :................ 1 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant •Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl •Sample size :............. 100 µl

1x96 tests 850.510.096 1x96 tests 850.520.096

2x96 tests 850.510.192 CD126/IL-6R/gp80 CD130/gp130 2x96 tests 850.520.192
•Range :...................... 31.25 pg / ml - 1000 pg / ml •Range :...................... 56.25 pg / ml - 1800 pg / ml
•Sensitivity :................ 5 pg / ml •Sensitivity :................ 15 pg / ml
•Incubation :................ 1 h 40 •Incubation :................ 1 h 40
•Samples :................... Serum, Plasma, Cell culture supernatant •Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl •Sample size :............. 100 µl
Référence: Reference:
Corbi P. et al., Eur J Cardiothorac Surg., 2000; 18 : 98 - 103 Corbi P. et al., Eur J Cardiothorac Surg., 2000; 18 : 98 - 103
Le Meur Y. et al., Nephrol. Dial. Transplant., 1999; 14(10) : 2420 - 2426

1x96 tests 850.800.096

2x96 tests 850.800.192 CD213a2/IL-13Rα2
•Range :...................... 156 pg / ml - 5000 pg / ml
•Sensitivity :................ 150 pg / ml
•Incubation :................ 3 h
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl

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 ELISA
Human Adhesion Molecules
1x96 tests 850.710.096 1x96 tests 850.570.096
2x96 tests 850.710.192 CD31 / PECAM-1 CD44/Pgp-1 2x96 tests 850.570.192
•Range :...................... 0.312 ng / ml - 10 ng / ml •Range :...................... 0.31 ng / ml – 10 ng / ml
•Sensitivity :................ 0.11 ng / ml •Sensitivity :................ 0.13 ng / ml
•Incubation :................ 2 h 45 •Incubation :................ 2 h 10
•Samples :................... Serum, Plasma, Cell culture supernatant •Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl •Sample size :............. 100 µl

1x96 tests 850.560.096 1x96 tests 850.540.096

2x96 tests 850.560.192 CD50 / ICAM-3 CD54 / ICAM-1 2x96 tests 850.540.192
•Range :...................... 0.62 ng / ml – 20 ng / ml •Range :...................... 0.25 ng / ml – 8 ng / ml
•Sensitivity :................ 0.3 ng / ml •Sensitivity :................ 0.1 ng / ml
•Incubation :................ 1 h 40 •Incubation :................ 1 h 40
•Samples :................... Serum, Plasma, Cell culture supernatant •Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl •Sample size :............. 100 µl
Référence:
Béchard D. et al., J. Immunol., 2001; 167 : 3099 - 3106
Sammons J. et al., Stem Cells 2000; 18 : 214 - 219

1x96 tests 850.530.096 1x96 tests 850.650.096

2x96 tests 850.530.192 CD62E / E-Selectin CD62L / L-Selectin 2x96 tests 850.650.192
•Range :...................... 1 ng / ml – 32 ng / ml •Range :...................... 1.6 ng / ml – 60 ng / ml
•Sensitivity :................ 0.5 pg / ml •Sensitivity :................ 1 ng / ml
•Incubation :................ 2 h 10 •Incubation :................ 1 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant •Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl •Sample size :............. 100 µl

1x96 tests 650.060.096 1x96 tests 850.550.096

CD62P / P-Selectin CD102 / ICAM-2 2x96 tests 850.550.192
•Range :...................... 2.19 ng / ml – 140 ng / ml •Range :...................... 0.75 U / ml – 24 U / ml
•Sensitivity :................ 1.3 ng / ml •Sensitivity :................ 0.2 U / ml
•Incubation :................ 2 h 15 •Incubation :................ 1 h 40
•Samples :................... Serum, Plasma, Cell culture supernatant •Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 10 µl •Sample size :............. 100 µl

1x96 tests 850.580.096

2x96 tests 850.580.192 CD106 / VCAM-1
•Range :...................... 1.56 ng / ml – 50 ng / ml
•Sensitivity :................ 0.6 ng / ml
•Incubation :................ 2 h 15
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
References:
Foussat A. et al., Blood, 2001; 98 : 1678 - 1686
Sammons J. et al., Stem Cells 2000; 18 : 214 - 219

www.diaclone.com 11
 ELISA
Human Soluble CD Antigens
1x96 tests 850.780.096 1x96 tests 850.730.096
2x96 tests 850.780.192 CD14 CD21 2x96 tests 850.730.192
•Range :...................... 6.8 ng / ml - 220 ng / ml •Range :...................... 3.12 U / ml - 100 U / ml
•Sensitivity :................ 6 ng / ml •Sensitivity :................ 3 U / ml
•Incubation :................ 3 h 45 •Incubation :................ 1 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant •Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl •Sample size :............. 100 µl

1x96 tests 850.590.096 1x96 tests 850.640.096

2x96 tests 850.590.192
•Range :...................... 1.187 U / ml - 38 U / ml
•Sensitivity :................ 0.7 U / ml
•Incubation :................ 2 h 40
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
CD86 CD138/Syndecan-1 2x96 tests 850.640.192
•Range :...................... 8 ng / ml - 256 ng / ml
•Sensitivity :................ 2.56 ng / ml
•Incubation :................ 1 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl
References:
Joensuu H. et al., Cancer Res., 2002; 62 : 5833 - 5842
Seidel C. et al., Blood, 2000; 95 : 388 - 392
Seidel C. et al., Blood, 2000; 96 : 3139 - 3146

1x96 tests 850.720.096

2x96 tests 850.720.192 CD141 / Thrombomodulin
•Range :...................... 1.625 ng / ml – 20 ng / ml
•Sensitivity :................ 0.38 ng / ml
•Incubation :................ 1 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 100 µl

www.diaclone.com 12
 ELISA
Mouse cytokines
1x96 tests 860.060.096 1x96 tests 860.050.096
1x96 tests 860.060.192 GM/CSF IFNγ 2x96 tests 860.050.192
•Range :...................... 15.6 pg / ml – 500 pg / ml •Range :...................... 31.25 pg / ml – 1000 pg / ml
•Sensitivity :................ 9 pg / ml •Sensitivity :................ 15 pg / ml
•Incubation :................ 3 h 45 •Incubation :................ 3 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant •Samples :................... Serum, Cell culture supernatant
•Sample size :............. 100 µl •Sample size :............. 100 µl

1x96 tests 660.010.096 1x96 tests 860.000.096

IL-1α IL-2 2x96 tests 860.000.192
•Range :...................... 7.8 pg / ml – 500 pg / ml •Range :...................... 15.6 pg / ml - 500 pg / ml
•Sensitivity :................ 6 pg / ml •Sensitivity :................ 5 pg / ml
•Incubation :................ 3 h 10 •Incubation :................ 3 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant •Samples :................... Serum, Cell culture supernatant
•Sample size :............. 50 µl •Sample size :............. 100 µl

1x96 tests 660.020.096 1x96 tests 660.030.096

IL-4 IL-5
•Range :...................... 3.9 pg / ml - 250 pg / ml •Range :...................... 78 pg / ml - 500 pg / ml
•Sensitivity :................ 3 pg / ml •Sensitivity :................ 5 pg / ml
•Incubation :................ 3 h 20 •Incubation :................ 3 h 10
•Samples :................... Serum, Plasma, Cell culture supernatant •Samples :................... Serum, Plasma, Cell culture supernatant
•Sample size :............. 50 µl •Sample size :............. 50 µl

1x96 tests 860.020.096 1x96 tests 860.030.096

2x96 tests 860.020.192
•Range :...................... 15.6 pg / ml - 500 pg / ml
•Sensitivity :................ 10 pg / ml
•Incubation :................ 3 h 45
•Samples :................... Serum, Cell culture supernatant
•Sample size :............. 100 µl
References:
Velders G A. et al., Blood, 2003; 10.1182
Yin F. et al., J. Biol. Chem., 2003; 278 : 21070 - 21075
IL-6 IL-10 2x96 tests 860.030.192
•Range :...................... 31.25 pg / ml - 1000 pg / ml
•Sensitivity :................ 20 pg / ml
•Incubation :................ 3 h 45
•Samples :................... Serum, Cell culture supernatant
•Sample size :............. 100 µl

1x96 tests 660.040.096 1x96 tests 860.040.096

IL-12 TNFα 2x96 tests 860.040.096
•Range :...................... 31.25 pg / ml - 2000 pg / ml •Range :...................... 31.25 pg / ml - 1000 pg / ml
•Sensitivity :................ 5.9 pg / ml •Sensitivity :................ 25 pg / ml
•Incubation :................ 3 h 10 •Incubation :................ 3 h 45
•Samples :................... Serum, Plasma, Cell culture supernatant •Samples :................... Serum, Cell culture supernatant
•Sample size :............. 10 µl •Sample size :............. 100 µl
Reference:
Velders G A. et al., Blood, 2003; 10.1182

www.diaclone.com 13

Rat cytokines
1x96 tests 865.020.096
2x96 tests 865.020.192
•Range :...................... 31.25 pg / ml - 1000 pg / ml
•Sensitivity :................ 15 pg / ml
•Incubation :................ 4 h 45
•Samples :................... Serum, Cell culture supernatant
•Sample size :............. 100 µl
www.diaclone.com
ELISA
1x96 tests 865.010.096
IFNγ 2x96 tests 865.010.192
•Range :...................... 31.25 pg / ml - 1000 pg / ml
•Sensitivity :................ 10 pg / ml
•Incubation :................ 3 h 45
•Samples :................... Serum, Cell culture supernatant
•Sample size :............. 100 µl
1x96 tests 865.000.096
IL-4 TNFα 2x96 tests 865.000.192
•Range :...................... 31.25 pg / ml - 1000 pg / ml
•Sensitivity :................ 15 pg / ml
•Incubation :................ 3 h 45
•Samples :................... Serum, Cell culture supernatant
•Sample size :............. 100 µl
14
 ELI-PAIRS

The Diaclone Eli-pair line allows you to carry out successful Elisa assays with optimised reagents at competitive prices. All our Do-
It-Yourself Eli-pairs are available in 1, 5, 10, 15, and 20 x 96 well-plate formats, they also include the corresponding number of vials
containing freeze-dried antigen to make one standard curve per plate.
Eli-pair components are exactly the same validated components as those used in our ready-to-use Diaclone Elisa kits enabling you
to occasionally validate your results by using a ready-to-use Diaclone Elisa kit.

Advantages of Diaclone Eli-pairs

Wide choice
Large range of antigens: cytokines, cytokine receptors, adhesion molecules, apoptosis related proteins, CD antigens
Several species: human, mouse and rat

High performance
Both natural and recombinant antigen specificity
High sensitivity
High reproductibility
Standard calibration to NIBSC/WHO (when available)

Iso quality
Accuracy and reliability guaranteed: all our reagents have been extensively validated according to the ISO 9001 quality system

Convenience
Demo kit available upon special request
Several sizes: available in 5, 10, 15 or 20 x 96 well-plate formats
Same components are used in the Diaclone Elisa kits

Eli-pair kit contents

Pre-titrated capture antibody
Pre-titrated biotinylated detection antibody
One standard per plate
Streptavidin-HRP
TMB
Detailed procedure including buffer composition

www.diaclone.com 15
 ELI-PAIRS
Human ELI-PAIRS (Including standard vials)
Specificity Size/Cat N°
5 x 96 tests 10 x 96 tests 15 x 96 tests 20 x 96 tests
Cytokines
IFN-γ 851 560 005 851 560 010 851 560 015 851 560 020
IL-1α 851 600 005 851 600 010 851 600 015 851 600 020
IL-1β 851 610 005 851 610 010 851 610 015 851 610 020
IL-2 851 500 005 851 500 010 851 500 015 851 500 020
IL-4 851 510 005 851 510 010 851 510 015 851 510 020
IL-5 851 670 005 851 670 010 851 670 015 851 670 020
IL-6 851 520 005 851 520 010 851 520 015 851 520 020
IL-7 851 680 005 851 680 010 851 680 015 851 680 020
IL-8 851 530 005 851 530 010 851 530 015 851 530 020
IL-10 851 540 005 851 540 010 851 540 015 851 540 020
IL-12 851 550 005 851 550 010 851 550 015 851 550 020
IL-13 851 630 005 851 630 010 851 630 015 851 630 020
TNF-α 851 570 005 851 570 010 851 570 015 851 570 020
Other Soluble Antigens
CD25/IL-2R 851 650 005 851 650 010 851 650 015 851 650 020
CD54/ICAM-1 851 590 005 851 590 010 851 590 015 851 590 020
CD62E/ELAM-1 851 580 005 851 580 010 851 580 015 851 580 020
CD106/VCAM-1 851 660 005 851 660 010 851 660 015 851 660 020
CD138/Syndecan-1 851 620 005 851 620 010 851 620 015 851 620 020
CD141/Thrombomodulin 851 690 005 851 690 010 851 690 015 851 690 020

Mouse ELI-PAIRS (Including standard vials)

Specificity Size/Cat N°
5 x 96 tests 10 x 96 tests 15 x 96 tests 20 x 96 tests
IFN-γ 861 050 005 861 050 010 861 050 015 861 050 020
IL-2 861 000 005 861 000 010 861 000 015 861 000 020
IL-6 861 020 005 861 020 010 861 020 015 861 020 020
IL-10 861 030 005 861 030 010 861 030 015 861 030 020

Rat ELI-PAIRS (Including standard vials)

Specificity Size/Cat N°
5 x 96 tests 10 x 96 tests 15 x 96 tests 20 x 96 tests
IFN-γ 872 000 005 872 000 010 872 000 015 872 000 020
IL-4 872 020 005 872 020 010 872 020 015 872 020 020
TNF-α 872 010 005 872 010 010 872 010 015 872 010 020

Human Elipair References:

CD138 Elipair : Seidel C. et al., Blood, 2000; 96 : 3139 - 3146 / IFNγ Elipair : Haller D. et al., Clin. Diagn. Lab. Immunol., 2002; 9 : 649 - 657 ; Sareneva T. et al., J. Immunol.,
2000; 165 : 1933 - 1938 / IL-10 Elipair : Haller D. et al., Clin. Diagn. Lab. Immunol., 2002; 9 : 649 - 657 / IL-12 Elipair : Puig-Kröger A. et al., Blood, 2001; 98 : 2175 - 2182
; Haller D. et al., Clin. Diagn. Lab. Immunol., 2002; 9 : 649 - 657 / IL-13 Elipair : Schramm G. et al., J. Biol. Chem., 2003; 278 : 18384 - 18392 / IL-1β Elipair : Haller D. et
al., Clin. Diagn. Lab. Immunol., 2002; 9 : 649 - 657 / IL-4 Elipair : Eglite S. et al., J. Immunol., 2000; 165 : 2183 - 2189 / IL-8 Elipair : Björkholm B M. et al., J. Biol. Chem.,
2002; 277 : 34191 - 34197 / TNFβ Elipair : Haller D. et al., Clin. Diagn. Lab. Immunol., 2002; 9 : 649 - 657.

www.diaclone.com 16
 ELISPOT

The Elispot (enzyme-linked immunoassay) technique was originally developed for the detection of individual B cell secreting
antibodies. However, this method has since been modified to detect and enumerate individual cells producing and secreting effector
molecules such as cytokines. It is the most appropriate method to analyse cytokine production in conditions closely comparable to the
in situ environment in a highly specific way. Elispot is the ideal tool to follow disease regression under appropriate treatment.

Diaclone Elispot assays are based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are
captured by coated antibodies avoiding diffusion in supernatant, protease deterioration or binding on soluble membrane receptors.
After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

Elispot constitutes an ideal tool in the following applications:

- TH1 / TH2 response
- Vaccine development
- Viral infection monitoring and treatment
- Cancerology
- Infectious diseases
- Autoimmune disease
- Transplantation

Advantages of Diaclone Elispot

Wide range
Several species: human, mouse and rat
Human Dual Elispot range: to monitor the production of two cytokines simultaneously in the same well

High performance
Fast procedure: only 3 h 30 hours after cell stimulation.
High sensitivity

Flexibility
Enzymatic or fluorescent detection
Mono-cytokine or Dual Elispot

Iso Quality
Accuracy and reliability guaranteed: all of our reagents have been extensively validated according to the ISO 9001 quality system

Convenience
Supplied with or without plates
Demo kit in a 1 x 96 well-plate format
Several sizes : available in 1, 5, 10, 15 or 20 x 96 well-plate formats
Storage box

Elispot Enzymatic Fluorospot Dual Elispot Matched Ab pairs

Enzymatic detection
Fluorescent detection
With non-sterile plates
With sterile plates
Without plates
With pre-coated plates AVAILABLE FOR CERTAIN PARAMETERS & QUANTITIES ( CONTACT US )

www.diaclone.com 17
 ELISPOT
Elispot Enzymatic Procedure Summary Elispot Enzymatic kit contents
1/ Capture Mab 2/ Add cells
Capture and detection antibodies
Streptavidin - Alkaline Phosphatase conjugated
BSA
Cell removal NBT/BCIP
3/ Add tracer biotinylated Mab 4/ Add strep AV-AP Buffers
Dry skimmed milk
With or without plates

5/ Add NBT/BCIP and visualise colored spots

Fluorospot Procedure Summary Fluorospot kit contents

1/ Capture Mab 2/ Add cells
Capture and detection antibodies
Streptavidin - Phycoerythrin conjugated
BSA
Cell removal Dry skimmed milk
3/ Add tracer biotinylated Mab 4/ Add strep AV-PE With or without plates

5/ Submit to UV and visualise colored spots

1/ Capture Mabs
Dual Elispot Procedure Summary 2/ Add cells
3/ Add tagged tracer Mabs
4/ Add conjugated enzyme
5/ Add substrates and visualise colored spots

anti-FITC Mab
HRP conjugated Dual Elispot kit contents
Tracer Mab FITC
Strep AV-AP
conjugated Capture and detection antibodies for cytokine 1
Capture and detection antibodies for cytokine 2
Streptavidin - Alkaline Phosphatase conjugated
Tracer biotinylated Mab Anti FITC - HRP conjugated mAb
Cytokine 1 Cytokine 2 BSA
NBT/BCIP and AEC
Buffers
Dry skimmed milk
Capture anti cyt1 MAb Capture anti cyt2 MAb
With or without plates
Strep AV-AP : Alkaline Phosphatase conjugated Strepatvidin, S1: NBT / BCIP, S2 : AEC

www.diaclone.com 18
 ELISPOT
Human Elispot Enzymatic
With PVDF plates
Specificity Size ( tests ) Without plates
Non-sterile Sterile
1 x 96 / 856 091 001 856 091 111
5 x 96 856 091 005 856 091 005 P 856 091 005 S
CD178/Fas Ligand 10 x 96 856 091 010 856 091 010 P 856 091 010 S
15 x 96 856 091 015 856 091 015 P 856 091 015 S
20 x 96 856 091 020 856 091 020 P 856 091 020 S
1 x 96 / 856 131 001 856 131 111
5 x 96 856 131 005 856 131 005 P 856 131 005 S
Granzyme B 10 x 96 856 131 010 856 131 010 P 856 131 010 S
15 x 96 856 131 015 856 131 015 P 856 131 015 S
20 x 96 856 131 020 856 131 020 P 856 131 020 S
1 x 96 / 856 051 001 856 051 111
5 x 96 856 051 005 856 051 005 P 856 051 005 S
IFN-γ 10 x 96 856 051 010 856 051 010 P 856 051 010 S
15 x 96 856 051 015 856 051 015 P 856 051 015 S
20 x 96 856 051 020 856 051 020 P 856 051 020 S
1 x 96 / 856 101 001 856 101 111
5 x 96 856 101 005 856 101 005 P 856 101 005 S
IL-1β 10 x 96 856 101 010 856 101 010 P 856 101 010 S
15 x 96 856 101 015 856 101 015 P 856 101 015 S
20 x 96 856 101 020 856 101 020 P 856 101 020 S
1 x 96 856 001 001 856 001 111
856 001 005
5 x 96 856 001 005 P 856 001 005 S
856 001 010
IL-2 10 x 96 856 001 010 P 856 001 010 S
856 001 015
15 x 96 856 001 015 P 856 001 015 S
856 001 020
20 x 96 856 001 020 P 856 001 020 S
1 x 96 / 856 011 001 856 011 111
5 x 96 856 011 005 856 011 005 P 856 011 005 S
IL-4 10 x 96 856 011 010 856 011 010 P 856 011 010 S
15 x 96 856 011 015 856 011 015 P 856 011 015 S
20 x 96 856 011 020 856 011 020 P 856 011 020 S
1 x 96 / 856 111 001 856 111 111
5 x 96 856 111 005 856 111 005 P 856 111 005 S
IL-5 10 x 96 856 111 010 856 111 010 P 856 111 010 S
15 x 96 856 111 015 856 111 015 P 856 111 015 S
20 x 96 856 111 020 856 111 020 P 856 111 020 S
1 x 96 / 856 021 001 856 021 111
5 x 96 856 021 005 856 021 005 P 856 021 005 S
IL-6 10 x 96 856 021 010 856 021 010 P 856 021 010 S
15 x 96 856 021 015 856 021 015 P 856 021 015 S
20 x 96 856 021 020 856 021 020 P 856 021 020 S
1 x 96 / 856 031 001 856 031 111
5 x 96 856 031 005 856 031 005 P 856 031 005 S
IL-10 10 x 96 856 031 010 856 031 010 P 856 031 010 S
15 x 96 856 031 015 856 031 015 P 856 031 015 S
20 x 96 856 031 020 856 031 020 P 856 031 020 S

www.diaclone.com 19
 ELISPOT
Human Elispot Enzymatic
With PVDF plates
Specificity Size ( tests ) Without plates
Non-sterile Sterile
1 x 96 / 856 081 001 856 081 111
5 x 96 856 081 005 856 081 005 P 856 081 005 S
IL-12 10 x 96 856 081 010 856 081 010 P 856 081 010 S
15 x 96 856 081 015 856 081 015 P 856 081 015 S
20 x 96 856 081 020 856 081 020 P 856 081 020 S
1 x 96 / 856 121 001 856 121 111
5 x 96 856 121 005 856 121 005 P 856 121 005 S
IL-13 10 x 96 856 121 010 856 121 010 P 856 121 010 S
15 x 96 856 121 015 856 121 015 P 856 121 015 S
20 x 96 856 121 020 856 121 020 P 856 121 020 S
1 x 96 / 856 041 001 856 041 111
5 x 96 856 041 005 856 041 005 P 856 041 005 S
TNF-α 10 x 96 856 041 010 856 041 010 P 856 041 010 S
15 x 96 856 041 015 856 041 015 P 856 041 015 S
20 x 96 856 041 020 856 041 020 P 856 041 020 S
Human Elispot Enzymatic references:
IFNγ Elispot : Gazagne A. et al., J. Immunol. Methods., 2003; 283 : 91-98 ; Sun Y. et al., J. Immunol. Methods, 2003; 272 : 23 – 34 ; Sauce D. et al., Blood, 2003; 102 : 1241
– 1244 ; Hudak S. et al., J. Immuno., 2002; 169 : 1189 – 1196 ; Alatrakchi N. et al., AIDS 2002; 16: 713 – 717 ; Ayyoub M. et al., J. Immunol., 2002; 168 : 1717 – 1722 ;
Sauce D. et al., Blood, 2002; 99(4) : 1165 – 1173 ; Pittet M. J. et al., J. Immunol., 2001; 166 : 7634- 7640 / IL-10 Elispot : Hudak S. et al., J. Immuno., 2002; 169 : 1189
– 1196 / IL-4 Elispot : Hudak S. et al., J. Immuno., 2002; 169 : 1189 – 1196 ; Sauce D. et al., Blood, 2002; 99(4) : 1165 – 1173

Mouse Elispot Enzymatic

With PVDF plates
Specificity Size ( tests ) Without plates
Non-sterile Sterile
1 x 96 / 862 031 001 862 031 111
5 x 96 862 031 005 862 031 005 P 862 031 005 S
IFN-γ 10 x 96 862 031 010 862 031 010 P 862 031 010 S
15 x 96 862 031 015 862 031 015 P 862 031 015 S
20 x 96 862 031 020 862 031 020 P 862 031 020 S
1 x 96 862 001 001 862 001 111
862 001 005
5 x 96 862 001 005 P 862 001 005 S
862 001 010
IL-2 10 x 96 862 001 010 P 862 001 010 S
862 001 015
15 x 96 862 001 015 P 862 001 015 S
862 001 020
20 x 96 862 001 020 P 862 001 020 S

Rat Elispot Enzymatic

With PVDF plates
Specificity Size ( tests ) Without plates
Non-sterile Sterile
1 x 96 / 867 011 001 867 011 111
5 x 96 867 011 005 867 011 005 P 867 011 005 S
IFN-γ 10 x 96 867 011 010 867 011 010 P 867 011 010 S
15 x 96 867 011 015 867 011 015 P 867 011 015 S
20 x 96 867 011 020 867 011 020 P 867 011 020 S

www.diaclone.com 20
 ELISPOT
Rat elispot enzymatic
With PVDF plates
Specificity Size ( tests ) Without plates
Non-sterile Sterile
1 x 96 867 021 001 867 021 111
867 021 005
5 x 96 867 021 005 P 867 021 005 S
867 021 010
TNF-α 10 x 96 867 021 010 P 867 021 010 S
867 021 015
15 x 96 867 021 015 P 867 021 015 S
867 021 020
20 x 96 867 021 020 P 867 021 020 S

Human Fluorospot
With PVDF
Specificity Size ( tests ) Without plates
Non-sterile Sterile
1 x 96 / 856 082 001 856 082 111
5 x 96 856 082 005 856 082 005 P 856 082 005 S
IFN-γ 10 x 96 856 082 010 856 082 010 P 856 082 010 S
15 x 96 856 082 015 856 082 015 P 856 082 015 S
20 x 96 856 082 020 856 082 020 P 856 082 020 S
1 x 96 / 856 002 001 856 002 111
5 x 96 856 002 005 856 002 005 P 856 002 005 S
IL-1β 10 x 96 856 002 010 856 002 010 P 856 002 010 S
15 x 96 856 002 015 856 002 015 P 856 002 015 S
20 x 96 856 002 020 856 002 020 P 856 002 020 S
1 x 96 856 012 001 856 012 111
856 012 005
5 x 96 856 012 005 P 856 012 005 S
856 012 010
IL-2 10 x 96 856 012 010 P 856 012 010 S
856 012 015
15 x 96 856 012 015 P 856 012 015 S
856 012 020
20 x 96 856 012 020 P 856 012 020 S
1 x 96 / 856 022 001 856 022 111
5 x 96 856 022 005 856 022 005 P 856 022 005 S
IL-4 10 x 96 856 022 010 856 022 010 P 856 022 010 S
15 x 96 856 022 015 856 022 015 P 856 022 015 S
20 x 96 856 022 020 856 022 020 P 856 022 020 S
1 x 96 / 856 032 001 856 032 111
5 x 96 856 032 005 856 032 005 P 856 032 005 S
IL-5 10 x 96 856 032 010 856 032 010 P 856 032 010 S
15 x 96 856 032 015 856 032 015 P 856 032 015 S
20 x 96 856 032 020 856 032 020 P 856 032 020 S
1 x 96 / 856 042 001 856 042 111
5 x 96 856 042 005 856 042 005 P 856 042 005 S
IL-6 10 x 96 856 042 010 856 042 010 P 856 042 010 S
15 x 96 856 042 015 856 042 015 P 856 042 015 S
20 x 96 856 042 020 856 042 020 P 856 042 020 S
1 x 96 / 856 052 001 856 052 111
5 x 96 856 052 005 856 052 005 P 856 052 005 S
IL-10 10 x 96 856 052 010 856 052 010 P 856 052 010 S
15 x 96 856 052 015 856 052 015 P 856 052 015 S
20 x 96 856 052 020 856 052 020 P 856 052 020 S
1 x 96 / 856 062 001 856 062 111
5 x 96 856 062 005 856 062 005 P 856 062 005 S
IL-12 10 x 96 856 062 010 856 062 010 P 856 062 010 S
15 x 96 856 062 015 856 062 015 P 856 062 015 S
20 x 96 856 062 020 856 062 020 P 856 062 020 S

www.diaclone.com 21
 ELISPOT
Human Fluorospot
With PVDF plates
Specificity Size ( tests ) Without plates
Non-sterile Sterile
1 x 96 / 856 092 001 856 092 111
5 x 96 856 092 005 856 092 005 P 856 092 005 S
IL-13 10 x 96 856 092 010 856 092 010 P 856 092 010 S
15 x 96 856 092 015 856 092 015 P 856 092 015 S
20 x 96 856 092 020 856 092 020 P 856 092 020 S
1 x 96 / 856 072 001 856 072 111
5 x 96 856 072 005 856 072 005 P 856 072 005 S
TFN-α 10 x 96 856 072 010 856 072 010 P 856 072 010 S
15 x 96 856 072 015 856 072 015 P 856 072 015 S
20 x 96 856 072 020 856 072 020 P 856 072 020 S
Human Fluorospot references :
IFNγ Fluorospot : Gazagne A. et al., J. Immunol. Methods., 2003; 283 : 91-98 / IL-2 Fluorospot : Gazagne A. et al., J. Immunol. Methods., 2003; 283 : 91-98 / IL-5 Fluorospot
: Gazagne A. et al., J. Immunol. Methods., 2003; 283 : 91-98 / IL-10 Fluorospot : Gazagne A. et al., J. Immunol. Methods., 2003; 283 : 91-98

Human Dual Elispot

With PVDF plates
Specificity Size ( tests ) Without plates
Non-sterile Sterile
1 x 96 / 874 040 001 874 040 111
5 x 96 874 040 005 874 040 005 P 874 040 005 S
IFN-γ/IL-2 10 x 96 874 040 010 874 040 010 P 874 040 010 S
15 x 96 874 040 015 874 040 015 P 874 040 015 S
20 x 96 874 040 020 874 040 020 P 874 040 020 S
1 x 96 / 874 010 001 874 010 111
5 x 96 874 010 005 874 010 005 P 874 010 005 S
IFN-γ/IL-4 10 x 96 874 010 010 874 010 010 P 874 010 010 S
15 x 96 874 010 015 874 010 015 P 874 010 015 S
20 x 96 874 010 020 874 010 020 P 874 010 020 S
1 x 96 / 874 020 001 874 020 111
5 x 96 874 020 005 874 020 005 P 874 020 005 S
IFN-γ/IL-5 10 x 96 874 020 010 874 020 010 P 874 020 010 S
15 x 96 874 020 015 874 020 015 P 874 020 015 S
20 x 96 874 020 020 874 020 020 P 874 020 020 S
1 x 96 / 874 000 001 874 000 111
5 x 96 874 000 005 874 000 005 P 874 000 005 S
IFN-γ/IL-10 10 x 96 874 000 010 874 000 010 P 874 000 010 S
15 x 96 874 000 015 874 000 015 P 874 000 015 S
20 x 96 874 000 020 874 000 020 P 874 000 020 S
1 x 96 / 874 050 001 874 050 111
5 x 96 874 050 005 874 050 005 P 874 050 005 S
IL-10/IL-2 10 x 96 874 050 010 874 050 010 P 874 050 010 S
15 x 96 874 050 015 874 050 015 P 874 050 015 S
20 x 96 874 050 020 874 050 020 P 874 050 020 S
1 x 96 / 874 030 001 874 030 111
5 x 96 874 030 005 874 030 005 P 874 030 005 S
IL-10/IL-4 10 x 96 874 030 010 874 030 010 P 874 030 010 S
15 x 96 874 030 015 874 030 015 P 874 030 015 S
20 x 96 874 030 020 874 030 020 P 874 030 020 S

www.diaclone.com 22
 ELISPOT MATCHED ANTIBODY PAIRS

Antibody Pairs are aimed to be used by researchers who have their own Eli-spot method but who wish to have the quality of
Diaclone antibodies. These antibodies are identical to those in the complete Eli-spot kits. They are available in a 10 x 96 well-plate
size. Elispot matched antibody pairs are exactly the same validated antibodies as those used in our ready-to-use Diaclone Elispot
kits which enables you to occasionally validate your results by using a ready-to-use Diaclone Elispot kit.

Advantages of Elispot matched Antibody Pairs

Several species: human, mouse and rat

Accuracy and reliability guaranteed : all of our reagents have been extensively validated according to the ISO 9001 quality system

Elispot matched Antibody Pairs kit contents

Pre-titrated capture antibody

HUMAN
Specificity Size ( tests ) Cat N°

IFN-γ 10 x 96 869 060 010

IL-1β 10 x 96 869 100 010
IL-2 10 x 96 869 000 010
IL-4 10 x 96 869 010 010
IL-5 10 x 96 869 110 010
IL-6 10 x 96 869 020 010
IL-10 10 x 96 869 030 010
IL-12 10 x 96 869 040 010
IL-13 10 x 96 869 120 010
TNF-α 10 x 96 869 050 010
CD178/FAS Ligand 10 x 96 869 080 010

MOUSE
Specificity Size ( tests ) Cat N°

IFN-γ 10 x 96 870 050 010

IL-2 10 x 96 870 010 010

RAT
Specificity Size ( tests ) Cat N°

TNF-α 10 x 96 871 030 010

IFN-γ 10 x 96 871 020 010

www.diaclone.com 23
 Also available on request, our Diaclone immunokit catalogue.

For further information, please contact your local distributor.

DIACLONE SAS
1, Bd Fleming BP1985 F-25020 BESANCON
FRANCE
E-mail : info@diaclone.com
Website : www.diaclone.com