Académique Documents
Professionnel Documents
Culture Documents
Module 1
Describe the chromatographic and spectroscopic instrumentation used and explain how it
operates to enable analysis
Explain the scientific principles underlying each chromatographic and spectroscopic
technique
Compare and contrast the various techniques giving advantages and disadvantages of each
Decide which technique(s) would be most appropriate for the analysis of a given
sample and explain the reasons for your decision
Give examples of the uses for each technique in analytical science, with particular emphasis
on analytical toxicology.
Chromatography
Introduction to separation science
The role of analytical chemistry in analytical science
Analytical techniques used in analytical science
The elements of analysis
The pros and cons of the various techniques
Maximising information and evidential value
What is separation science? Basis of separation
Terminology
Definition of chromatography
Chromatographic theory
The mechanisms of separation Introducing the major separation techniques used in analytical
chemistry
Exploring the basis of separation - intermolecular interactions (hydrogen bonding, van der
Waals forces, ionic interactions)
High Performance Liquid Chromatography (HPLC) Introducing instrumentation
Normal phase HPLC
Reverse phase HPLC
Mechanisms of separation operating in HPLC
Stationary and mobile phases
Uses of HPLC
Gas Chromatography (GC) Instrumentation
Carrier gases
Injectors
Chromatographic columns and stationary phases
Use of temperature
Detectors
How to manipulate separation
Uses of GC (including headspace analysis)
Chirality
Separation of enantiomers
Direct and indirect methods
Sample Handling Solvent and solid state extraction
Manipulation of pH to aid separation
Analysis of conjugates
Quality Assurance
Traceability
Reference materials and drug standards.
Quality Control procedures
Quality Assurance
Method validation
Uncertainty budget fit for purpose
International Committee on Harmonisation (ICH), uncertainty and guidelines
(e.g. ISO 17025)
Laboratory accreditation (e.g. UKAS )
Solubility
Intermolecular interactions
Functional groups and chemical nomenclature
MODULE 2
BIOCHEMICAL TOXICOLOGY
Steady-state
Dose regimens
module will include assessed coursework, data evaluation, report preparation and presentation,
tutorials and workshops.
At the end of the module the student should be able to:
Understand the clinical and forensic issues associated with the laboratory investigation of
acute and chronic poisoning, including drug and substance misuse
Understand the main clinical and biochemical features associated with acute and chronic
poisoning due to the ingestion of important drug and chemical poisons
Understand the pharmacokinetics and metabolism of important drug and chemical poisons
and relationship to acute and chronic toxicity, including sudden or unexpected deaths
Critically review and evaluate the significance of analytical data from human poisoning cases
Present the significance of analytical findings accurately and concisely, including court
reporting
Demonstrate an understanding of the various analytical approaches to the detection and
quantitation of drugs and metabolites in biological fluids and tissues
Understand the role of both laboratory testing and point of care testing in the management
and detection of drug misuse
Understand the principles and practice of therapeutic drug monitoring
Cocaine
Opiates
The clinical and forensic toxicology of opiates including morphine , heroin , codeine and
dihydrocodeine
Laboratory techniques used in the investigation of suspected opiate abuse and overdosage
The pharmacokinetics and metabolism of opiates
Acute and chronic toxicity and fatalities
Opioids
The clinical and forensic toxicology of important opioid drugs including methadone,
dextropropoxyphene and buprenorphine
Laboratory techniques used in the investigation of suspected opioid abuse and overdosage
The pharmacokinetics and metabolism of opioids
Acute and chronic toxicity and fatalities
Newer drugs: GHB, Ketamine and Piperazines
The clinical and forensic toxicology of GHB, ketamine and piperazines
Laboratory techniques used in the investigation of suspected abuse and overdosage
The pharmacokinetics and metabolism of GHB, ketamine and piperazines
Acute and chronic toxicity and fatalities
Benzodiazepines
The clinical and forensic Toxicology of benzodiazepines and related hypnotic drugs such as
zopiclone, zolpidem and related drugs.
Laboratory techniques for the detection and measurement of these drugs in biological fluids
in the investigation of suspected abuse and overdose.
The pharmacokinetics and metabolism of this group of drugs and relationship to acute and
chronic toxicity and suspected fatalities will be reviewed
Psychotropic drugs
The clinical and forensic toxicology of major groups of psychotropic drugs such as the
antidepressants and antipsychotics
Laboratory techniques for the detection and measurement of these drugs in biological fluids
in the investigation of suspected poisoning and overdosage
The pharmacokinetics and metabolism of important drugs and relationship to acute and
chronic toxicity and suspected fatalities will be reviewed
Pesticides: OPs , Paraquat , Phosphides , Strychnine
The clinical and forensic toxicology of important pesticides
Laboratory techniques for detection and measurement of these agents in biological fluids in
the investigation of suspected poisoning
The pharmacokinetics and metabolism of these agents and relationship to acute and chronic
toxicity and suspected fatalities will be reviewed
The role of the laboratory investigations in the diagnosis and management of suspected
poisoning
The role of the laboratory in the investigation of sudden or unexpected deaths
Best practice for the provision of laboratory investigations including the techniques used and
their application to patient care
Laboratory visit
To be arranged.
Workshops
Investigation of drug misuse - analytical, clinical, occupational and forensic aspects .
Introduction to trends in drug misuse
Specimen collection , adulteration and chain of custody
Analytical techniques for screening and confirmation
Post - mortem Toxicology
The legal system and introduction to the role of the coroner /medical examiner, coroners
officers and police.
The post-mortem examination , post mortem changes and physiology
and collection of specimens . The role of the pathologist
Laboratory investigation of unexpected or sudden death What to look for ,
Selection of analytical techniques, Choice of specimens and Interpretation
of results .The role of the toxicologist .
Problems and pitfalls in the interpretation of post mortem findings
for drugs and alcohol e.g. putrefaction , post mortem redistribution
and exhumation - some illustrative cases .
Investigation of heroin related deaths, mechanisms of death and current clinical and
forensic issues
Use of hair analysis in understanding historical drug use in the deceased .
Forensic Evidence
Writing court reports and presentation of evidence in court.
Exercise based on a real case .
MODULE 4
Mass analysers
Linear quadrupoles
3D ion traps
2D ion traps
Magnetic sector instruments (isotope ratio mass spectrometry)
Time Of Flight (TOF) including reflectron and delayed extraction
MS in Analytical Toxicology
Chromatography and mass spectrometry refresher
Gas Chromatography-Mass Spectrometry (GC-MS)
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Liquid Chromatography-Time Of Flight Mass Spectrometry (LC-TOFMS)
Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometry (GC-C-IRMS)
Direct Analysis in Real Time (DART)
n
MS techniques in practice
Selected reaction monitoring
Product ion scanning
Precursor ion scanning
Neutral loss scanning
Effect of time domain & energies
Collision Activated Dissociation (CAD)
Software
Interpretation of Mass Spectra
Isotope Abundance
Accurate Mass And Elemental Composition`
Fragmentation Pathways
Method Development and Validation
Infusion
Chromatography
Instrument Tuning
Matrix Effects
Identification Criteria for Confirmatory Purposes
Quantification
Large Molecule Mass Spectrometry
Protein identification
Analysis of intact and digested proteins
Peptide sequencing
Peptide mass mapping
Post-translational modifications
Examples, e.g. Insulin
Sample Extraction using Reverse Phase, Normal Phase, Ion-Exchange and Mixed Mode
Cartridges
Competitive Immunoassay for Recreational Drugs
Handling of On-site Testing Devices, including alcometers
Mass Spectrometry including infusion and hyphenated Separation Techniques
Tuning the Mass Spectrometer
Resolution, sensitivity and mass accuracy
Mass Range, Scan and Selected Ion Monitoring
Sensitivity and speed
MS/MS selected reaction monitoring
Identification of a Substance in a Matrix
Quantification of target analytes
MODULE 5
Project
All students have an analytical toxicology placement, either of a clinical or forensic nature between
May and August. The total length of the placement is 16 weeks, which includes preparation of the
project report.
This academic year, placements will begin in May.
Students generally undertake the placement in a laboratory or organisation exterior to King's,
although staff at King's also offer placements that may be of interest to students and some
organisations offer placements where some of the practical work takes place in the organisation and
some takes place at Kings.
You must recognise that it is not always possible to assign you your first or second (or sometimes
even your third) placement choice, although we will do our best. If you refuse to select an alternative
placement from those on offer, one will be assigned to you and you will be expected to take it,
irrespective of where in the UK it is located.
Security clearance is mandatory for placements in some laboratories. The host laboratory will arrange
for the security clearance.
It should be noted that we reserve the right to refuse an external placement. In these circumstances,
the individual will carry out their placement within King's.
Assessment
Assessment will be via a written review of the literature pertaining to the research topic; submission
of a journal article; presentation of a poster with oral questioning by assessors.
Literature Review
Research Article, in the style of a journal publication
Poster presentation, including oral examination
Supervisors evaluation