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Chapter 5
Embryonic Tissues
An embryo begins as a single
cell that divides into many cells
that eventually forms 3 Primary
Layers:
ectoderm (outer layer)
forms epidermis and
nervous system
endoderm (inner layer)
forms digestive glands
and the mucous
membrane lining
digestive tract and
respiratory system
mesoderm (middle layer)
Forms muscle, bone,
blood and other organs.
Histotechnology
Preparation of specimens for histology:
preserve tissue in a fixative to prevent decay (formalin)
dehydrate in solvents like alcohol and xylene
embed in wax or plastic
slice into very thin sections only 1 or 2 cells thick
float slices on water and mount on slides and then add color with
stains
Sectioning an organ or tissue reduces a 3-dimensional structure to a 2dimensional slice.
Planes of Section
Longitudinal section
tissue cut along the
longest direction of
a structure
Cross section
tissue cut
perpendicular to the
length of a structure
Oblique section
tissue cut at an
angle between a
cross section and a
longitudinal section
Slicing through a
boiled egg is similar
to sectioning a cell
and its nucleus.
Slices 1 and 5
miss the yolk.
Yolk appears larger
in section 3 than in
sections 2 and 4.
B
Image A is a cross
section of a curved
tubular structure like a
blood vessel or a
section of intestine.
Image B is a
longitudinal section of a
spiraling, tubular
structure like a sweat
gland.
Notice what a single
slice could look like.
Naming Epithelia
Epithelia are named for:
the number of layers of
cells
simple epithelium =
one layer of cells
stratified epithelium =
more than one layer of
cells
pseudostratified
epithelium = simple
that looks stratified
the shape of cells at the
surface
squamous
cuboidal
columnar
transitional
surface modifications
cilia
microvilli
keratinization
Simple
Squamous
Epithelium
Single row of
squamous (flat)
cells.
Can allow rapid
diffusion of
substances or
secretion of fluid.
Example: lining of
blood vessels or
lining of lung
alveoli
Goblet Cell
Nucleus
Pseudostratified Epithelium
Cilia
Goblet
Cells
Basal
Cells
Single row of cells all attached to basement membrane
Not all cells reach the free surface
nuclei of basal cells give a stratified appearance
Secretes and propels respiratory mucus
Example: lining of trachea
Mucous Membranes
Stratified Epithelia
Composed of more than one layer of cells.
Always named for shape of surface cells.
Deepest cells sit on basement membrane and are
the source of replacement cells for the epithelium.
Keratinization:
keratinized epithelium has surface layer of dead
cells that contain abundant protein and are
surrounded by lipids
nonkeratinized epithelium has living cells with
nuclei in all layers
Nonkeratinized
Stratified
Squamous
Stratified epithelium of
living cells forms an
abrasion-resistant,
moist, slippery layer.
Examples: lining of the
mouth, esophagus,
vagina
living epithelial
cells
connective tissue
Transitional
Epithelium
Stratified
epithelium with
rounded (domed)
surface cells.
Stretches to allow
storage of urine.
Example: urinary
bladder.
Intercellular Junctions
Tight Junctions
Tight junctions completely encircle the cell (like a
sweat band around a persons head)
Tight Junctions form a zipper-like pattern of
complementary grooves and ridges that prevent
substances and bacteria from passing between
cells.
Tight Junctions
Desmosomes
Attachment between cells that holds them
together against mechanical stress (shearing
forces).
A mesh of protein filaments connects integral
membrane proteins and cytoskeletal proteins.
Abundant in muscle and skin
Hemidesmosomes attach
cells to the basement
membrane.
Desmosome
Hemidesmosome
Gap Junctions
Also called communicating junctions.
Cluster of tube-shaped transmembrane proteins
that make channels between cells.
Small solutes and electrical signals pass directly
from cell to cell and can synchronize the activity
of groups of cells.
Found in embryos, cardiac
muscle and smooth muscle.
Gap Junction
Glands
Glands secrete substances for elimination or for use
elsewhere in the body
Glands are composed predominantly of epithelial tissue
Exocrine glands maintain connection to the surface
through a duct (examples: sweat glands, salivary
glands)
Endocrine glands have no ducts but secrete their
products (hormones) onto capillaries for absorption
directly into bloodstream (pituitary, adrenal) or into
interstitial fluid
Mixed organs have both types of glands:
pancreas secretes digestive enzymes into ducts and
hormones into blood
gonads release gametes into ducts and secrete
hormones into blood
Serous
thin, watery secretions such as sweat, milk, tears
and digestive juices.
Mucus
the sticky secretion called mucus is a glycoprotein,
mucin, that absorbs water
Mixed Glands secrete both serous fluid and mucus
Note: Mucus is a noun. Mucous is an adjective.
Mucus is secreted by mucous glands.
Cellular mechanisms of glandular secretion include:
1) merocrine
2) apocrine
3) holocrine
Merocrine Secretion
Duct
Acinus
Cells of Merocrine
Glands release their
product by exocytosis.
Clusters of secretroy
cells are called acini.
Products include tears,
sweat, milk, pancreatic
enzymes, gastric
enzymes and acid
Holocrine Secretion
http://www.lab.anhb.uwa.edu.au/mb140/CorePages/Epithelia/Epithel.htm
Connective Tissue
Connective Tissues consist of widely spaced cells
suspended in an abundant extracellular matrix.
The volume of the extracellular matrix is greater than the
volume of the cells.
A Classification
Scheme for
Connective
Tissues
Loose (areolar)
Dense
Dense Regular
Dense Irregular
Reticular
Elastic
Hematopoietic
Bone
a proteoglycan
Proteoglycan Aggregate
Loose (Areolar)
Connective
Tissue
Scattered cells include
thin fibroblasts,
macrophages and mast
cells.
Loose arrangement of
thick, wavy collagen fibers
(C) and thin dark-staining
elastic fibers (EF).
Found under some types
of epithelia including the
mesentary of the digestive
tract.
Contains nerves and
blood vessels.
EF
C
blood
vessel
Reticular Tissue
Elastic
Tissue
Cross section of
the aorta shows
alternating layers
of muscle,
collagen fibers and
thinner, darker
staining elastic
fibers.
Special stains
containing
solutions of metals
like silver are
needed to
visualize elastic
fibers.
erythrocyte
platelets
Cartilage
Supportive connective tissue with a rubbery
matrix
Cartilage is an avascular tissue (no blood
vessels in the matrix) so cells rely on diffusion
from a surrounding vascular membrane, the
perichondrium, to deliver nutrients and
remove wastes. (injured cartilage heals
slowly if at all)
3 types of cartilage distinguished by the fibers
of the extracellular matrix:
hyaline
elastic
fibrocartilage
Hyaline Cartilage
Fibrous, vascular
capsule called the
perichondrium covers a
clear, non-fibrous
matrix.
Chondrocytes produce
the cartilage matrix.
Each cell is in a pit
called a lacuna.
Hyaline cartilage
covers the ends of
bones at all movable
joints plus the sternal
ends of ribs and is the
supportive material in
nose, larynx, trachea,
bronchi.
Elastic Cartilage
Fibrous, vascular
perichondrium
covers elastic
cartilage
The cartilage
matrix has a
weblike mesh of
elastic fibers
among the
lacunae
Provides flexible,
resilient, support
for the external
ear and epiglottis
Fibrocartilage
Bone
Bone matrix stores the minerals calcium and
phosphorus.
Compact bone provides physical support for
leverage during muscle contraction.
Spongy bone, also called trabecular or
cancellous, fills the ends of long bones and
supports the bone marrow.
Compact bone always covers spongy bone.
Compact Bone
Muscle Tissue
Cells that respond to stimuli by contracting
Function is to exert physical force on other
tissues
move bones
moves blood through vessels
expel urine and feces
Skeletal Muscle
Cardiac Muscle
Smooth Muscle
Neurons