PCR AND RFLP WORKSHEET

Use p 296-308 Name

1.The amplification of DNA sequences by repeated cycles of stand separation
andreplication is called?
2.Before the advent of PCR, the only way of replicating a gene fragment to make
copies of a DNA sequence was to grow it in a
3.How are the DNA strands separated in PCR?
4.How is this different from the way the DNA strands are normally
separated?
5.Normally DNA polymerase III utilizes RNA primers to initiate transcription. In
PCR what is used? whv?
6.At what temperature is transciption conducted in PCR?
7.What is used rather than DNA polymerase?
8.Why is DNA polymerase not used?
9.What is unique about Taq polymerase?
1O.The growth of the number of copies target DNA utilizing PCR is described as
11.Taq polymerase copies the DNA of the gene and continues to copy the
complimentary codes to the primer. The resultant stands
are described as
12.What are the variable length strands?

13.What are the constant length strands ?

14.Why is PCR a vital tool for forensic criminal investigations?

15.Why might PCR can be used to identify HIV rather than antibodies?
16.How is PCR custom made to indicate HIV?
17.Who first proposed the PCR process and what award was received?
18.Any difference in DNA sequence coding or non-coding that can be detected
between individuals is called
l9.What does RFLP stand for?
2O.Individuals of the same species would be except for _ twins
2l.Innoncoding regions of the DNA, what would a scientist look for?
22.In coding regions of the DNA, what polymorphisms would a scientist look
for?_
23.What is the first step in conducting RFLP?
24.Why do the fragments appear as a smear on the gel?
25.Why is a special chemical applied to the gel?
26.What is the name for the technique utilized to transfer the DNA from the gel to
the nylon membrane?_
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2T.Whentranferring the DNA to nylon, the positive charge would be located
here_
28.If two fragments cut by the restiction endonuclease are of the same length, yet
are not the same gene, how can you indicate these two as distinct?_
29.What is special about the complementary nucleotide probes that the nylon is
immersed in?_
30.When a bond occurs between the probe and the DNA this is called
31.When the nylon membrane is placed against anX-ray film, the resultant pattern
is called an
32.Is it better to use PCR or RFLP to analyze a drop of blood tn a car?
33.Is it better to use PCR or RFLP if a fast result is needed?
34.Is it better to use PCR or RFLP a whole genome needs analysis?
35.Is it PCR or RFLP thatuttlizes restriction endonucleases?
36.The fina1 products of PCR are viewed on a while the final
products of RFLP are viewed on a
37.The DNA sequencing technique based on DNA replication that uses
dideoxynucleoside triphosphates is called
38.What four things are needed for the Sanger method to conduct DNA
replication?
39.What is special about the dideoxy analogue?
40.What is another name for the Sanger method?
41.State the exception from the standard Sanger technique that the human genome
project utilized
42.The process by which an individual' s DNA is scanned for genetic mutations is
called
43.The pattern of bands on a gel, originating from RFLP analysis ro PCR that is
unique to each individual iscalled_
44.An organism in which foreign DNA has been artificially incorporated into its
genome_
45.The alteration of a genetic sequence in an organism to prevent or treat a genetic
disorder
46.The poisonous substance produced by Bacillus thurigienszs which acts as a
natural herbicide
47 .The signal molecules that amplify pain sensation via the neurological pathway _
48. The plasmid found in Agrobacterium tumefaciens bacteriathat is abvle to enter
a plant
49.Shor1 stretches of DNA or RNA that recognize and deactiveate complementary
mRNA molecules _
50 Signal molecules that dampen pain sensation via a neurological pathway-