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Abstract
Fossil fuels create a negative impact on our environment as greenhouse gas emissions are harmful. Production of ethanol (as an
alternative fuel) from food and agricultural waste was done in this
research by bio-processing. Wastes from fruits, such as banana,
orange, pineapple and pea peels were subjected to simultaneous
saccharification and fermentation for 7 days by co-culture of
Aspergillus niger and Saccharomyces cerevisiae. The ethanol yield
was determined at 24 hours interval. The results of the study showed
that after 7 days of fermentation, pineapple peels had the highest
biomass yield, followed by banana peels, orange peels, pea peels. The
optimal ethanol yields were 8.34% v/v, 7.45 % v/v, 3.98 % v/v and
2.58 % v/v for pineapple, banana, orange and pea peels respectively.
These indicate that pineapple and banana peels ethanol yields were
significantly higher than orange and pea peel ethanol yield.
Keywords: Saccharomyces cerevisiae, Aspergillus niger, ethanol
production, fruits waste, fermentation, sachcharification.
1. Introduction
Due to rapid exhaustion of fossil fuels there is an urgent need to resort to alternative
fuels e.g. ethanol. The first large scale use of ethanol as a fuel happened during the
early 1900s when petroleum supplies in Europe were short. Though ethanol is
conventionally produced from petroleum by-products, bio ethanol can alternatively be
produced by fermentation technology using renewable raw material.
Saccharomyces cerevisiae is the most popular organism used for ethanol
production due to its high ethanol yield and high tolerance. Now a days, crops are the
main source used for ethanol production. To achieve significant economic and
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Girisha Malhotra et al
environmental benefit large amount of food wastes can be utilized to produce ethanol.
Utilization of fruit waste for bioethanol production is one of the best options. One
example of raw material is pineapple waste that is converted to bioethanol (Hossain et
al., 2008). The wastes contain valuable components such as sucrose, glucose, fructose
and other nutrients (Sasaki et al. 1991). Lignocellulose is the major structural
component of woody plants and non woody plants.
The use of mango peel as a source of pectin and fibre production also has been
reported (Pandia et al., 2004). Grohmann et al. (1994; 1995; 1996; 1998) previously
reported ethanol production from orange peel. Ethanol production from banana
(Manikandan et al., 2008) and pineapple peels (Ban-koffi and Han, 1990) were also
investigated. Dried orange peels have a high content of pectin, cellulose and hemi
cellulose, which make it suitable as fermentation substrate when hydrolyzed. Insoluble
carbohydrates are present in the cell walls of the peels, particularly in the form of
pectin, cellulose and hemicellulose.
2. Literature Review
Ethanol was first prepared synthetically in 1826, through the independent effort of
Henry Hennel in Britain and S.G in France. Michael Faraday prepared ethanol by the
acid-catalysed hydration of ethylene in 1828, in a process similar to that used for
industrial synthesis of ethanol today. Almost all ethanol is being produced by
fermentations using S. Cerevisiae. Karsch et al. (1983) evaluated the potential of
Zymomonas mobilis and Saccharomyces cerevisiae for ethanol production from
glucose both aerobically and anaerobically. S. cerevisiae commonly known as Bakers
yeast has the ability to ferment a sugar solution poorly supplied with oxygen, resulting
in the formation of alcohol and carbon dioxide. Zymomonas mobilis degrades sugars to
pyruvate using the Entner-Doudoroff pathway. The pyruvate is then fermented to
produce ethanol and carbon dioxide as the only products (Farombi and Britton, 1999).
Also A. niger produces enzymes such as amylase, amyloglucosidase, cellulases,
lactase, invertase and pectinases.
Maximum saccharification was achieved by hydrolysing banana-waste cellulose
with a cellulase enzyme from Trichoderma reesei QM 9414. A yield of 138% and
078% (v/v) and 445% and 611% ethanol (mg g1reducing sugars) was achieved
from cellulose and acid hydrolysed (25% at 15 psi for 15 min) banana peels,
respectively. Grohmann et al. (1998) observed that E. Chrysanthemi EC16 contains the
PET operon from Zymomonas mobilis on the plasmid pLOI555 which increases the
organisms ethanol production and decreases the final concentration of co-products
(Beall and Ingram 1993). Escherichia chrysanthemi EC16 fermentations of sugar beet
pulp produced 1.97 % (w/v) ethanol, less than the 2.55 % (w/v) ethanol produced by E.
Coli KO11 on the same substrate. In a study by Jayant Mishra et al. (2012), ethanol
production from fruit peels of pineapple, orange and sweet lime was investigated.
Total amount of sugar in pineapple, sweet lime and orange was 0.5, 1 and 0.8%
respectively. In the solid state fermentation, pineapple agro residue gives a maximum
yield around 2.16% with yeast. With a change of strain to C. albicans, pineapple still
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gives a high yield of 1.08% for group A in 50 ml capacity. Pineapple gives a maximum
yield of 1.87% with S. cerevisae. Lavarack B. P. et al. (2002) tried dilute acid
hydrolysis of bagasse for conversion of hemicellulose to xylose, glucose, arabinose,
acid soluble lignin and furfural.
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60
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40
30
%residual sugar
20
Ethanol g/l
10
0
24
48
72
96
120
144
168
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Girisha Malhotra et al
35
30
25
20
15
%residual sugar
10
Ethanol g/l
5
0
24
48
72
96
120
144
168
192
35
30
25
20
15
%residual sugar
10
Ethanol g/l
5
0
24
48
72
96
50
40
30
%residual sugar
20
Ethanol g/l
10
0
24
48
72
96
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5. Conclusion
From the experiments, it is proved that the ethanol yield could be produced from fruit
and vegetable waste as the substrates. Maximum activity is obtained by using
pineapple peel as a substrate at 28C. Bioconversion of vegetable and fruits waste into
ethanol was done. Various analytical tests were performed to estimate total sugar,
fermentable sugar, residual sugars and protein content before and after fermentation.
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