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(QUANTITATIVE ANALYSIS)
Denise Caramoan, Christine Cerrero, Joshua Adrian Crisostomo, Joseph Cua, Coleen David
ABSTRACT
Carbohydrates are one of most important organic compounds found in living organisms
because it is a major source of energy. It is distributed among plants and animals and can even be
found in animal tissues one of which is the liver. The objective of this experiment, particularly
the quantitative test, is to determine the amount of carbohydrates in a sample using Nelsons
method.
INTRODUCTION
Carbohydrates are compounds that
possess a large quantity of hydroxyl groups
and are a main source of energy among
living organisms. Being known as
saccharides, they are classified as
monosaccharides, oligosaccharides, and
polysaccharides, which are based on the
amount of carbohydrate groups that they
have which are the following respectively:
simple sugars that cannot be hydrolyzed,
one containing 2 to 10 monosaccharide
units, and one having more than 10 units of
saccharides.
Monosaccharides can be classified
further into to: pentoses and hexoses with
their prefixes denoting the amount of carbon
atoms that they have. Also, they can be
classified as aldoses and ketoses one having
an aldehyde group and the other containing
a ketone group respectively.
Glucose
Distille
Unknow
#
1
2
3
4
5
6
7
8
Standar
d (mL)
0
0.1
0.2
0.4
0.6
0.8
1.0
0
d Water
(mL)
1.0
0.9
0.8
0.6
0.4
0.2
0
0.6
Table 1
n Sample
(mL)
0
0
0
0
0
0
0
0.4