V 6.1 EUCAST QC Tables Routine and Extended QC

European Committee on Antimicrobial
Susceptibility Testing
Routine and extended internal quality control
for MIC determination and disk diffusion
as recommended by EUCAST
Version 6.1, valid from 2016-03-01
This document should be cited as

"The European Committee on Antimicrobial Susceptibility Testing. Routine and extended internal quality
control for MIC determination and disk diffusion as recommended by EUCAST.
Version 6.1, 2016. http://www.eucast.org."
General
Notes
Changes
Page
1
2
Routine quality control

Escherichia coli ATCC 25922
Pseudomonas aeruginosa ATCC 27853
Staphylococcus aureus ATCC 29213
Enterococcus faecalis ATCC 29212
Streptococcus pneumoniae ATCC 49619
Haemophilus influenzae ATCC 49766
Campylobacter jejuni ATCC 33560
Control of the inhibitor component of -lactam--lactamase inhibitor
combination disks
Page
4
6
7
9
10
12
13
14
Extended quality control for detection of resistance mechanisms

with disk diffusion
ESBL production in Enterobacteriaceae
Methicillin resistance in Staphylococcus aureus
VanB -mediated glycopeptide resistance in enterococci
High-level aminoglycoside resistance in enterococci
Reduced susceptibility to -lactam agents due to PBP mutations in
Haemophilus influenzae
Page
16
16
16
16
17
EUCAST QC Tables v. 6.1, valid from 2016-03-01
Notes
1. In EUCAST quality control (QC) tables, both ranges and targets are listed. Repeat testing of
EUCAST quality control strains should yield individual MIC and zone diameter values randomly
distributed within the recommended ranges. If the number of tests is 10, the mode MIC should be the
target value and the mean zone diameter should be close to the target value.
2. For access to ISO standard documents, see http://www.eucast.org/documents/external_documents/.
3. EUCAST quality control strains for routine QC are used to monitor test performance. Control tests
should be set up and checked daily, at least for antibiotic agents which are part of routine panels. For
analysis of the QC test results, see EUCAST Disk Diffusion Manual.
4. Specific -lactamase-producing strains are recommended to check the inhibitor component of lactam--lactamase inhibitor combination disks. This should be part of the routine QC. The active
component is checked with the standard QC strains.
5. EUCAST quality control strains for extended QC are complementary to the EUCAST routine quality
control strains. These strains are recommended for detection of specific resistance mechanisms
(ESBL, MRSA, VRE, HLGR and PBP mutations) and are used to check that routine susceptibility
testing will result in the correct S, I and R categorisation. Extended QC should be performed with any
change in the susceptibility testing system (with each new batch of disks or medium) and/or monthly.
Changes from previous version

Version 6.1
2016-03-01
Changes
Cells containing a change or an addition from EUCAST QC Tables v. 6.0
are marked blue.
ATCC 25922
QC range updated: Meropenem (zone diameter). The range is updated
in collaboration with CLSI.
ATCC 27853
QC range updated: Cefepime (zone diameter). The range is updated in
collaboration with CLSI.
Quality control strains for QC range added: Streptomycin with E. faecalis ATCC 51299.
detection of resistance
mechanisms
Version 6.0
2016-01-01
General
ATCC 25922
ATCC 35218
ATCC 27853
Changes
Cells containing a change or an addition from EUCAST QC Tables v. 5.0
are marked yellow.
Clearer distinction between routine and extended QC.
New format for extended QC tables.
Zone diameter targets are spelled out for ranges with no single target
value, e.g. range 19-26 and target 22-23 mm (instead of 23 mm).
QC ranges added: Ceftobiprole (zone diameter) and ceftolozanetazobactam (MIC and zone diameter).
New comments: Comment 9.
Revised comments: Comment 10.
Table moved to "Control of the inhibitor component of inhibitorcombination disks".
QC ranges added: Ceftolozane-tazobactam (MIC and zone diameter).
New comment: Comments 5 and 7.
Revised comment: Comment 6.
QC ranges added: Dalbavancin, oritavancin and tedizolid (MIC).

Ceftobiprole (zone diameter).
Revised comments: Comments 5-7.
ATCC 29212
QC ranges added: Streptomycin (zone diameter).
New comments: Comment 5-6.
ATCC 49619
QC ranges added: Dalbavancin, oritavancin and tedizolid (MIC).
Revised QC ranges: Ceftaroline and ceftobiprole (zone diameter
changed from IP to "-").Trimethoprim-sulfamethoxazole (zone diameter).
Revised comments: Comments 5-7.
NCTC 8468
Excluded from 2016. H. influenzae ATCC 49766 is recommended for
routine QC.
ATCC 49766
QC ranges added: Ceftibuten, ceftriaxone, ciprofloxacin, levofloxacin,
moxifloxacin and ofloxacin (zone diameter).
Revised QC ranges: Amoxicillin-clavulanic acid (zone diameter).
Ceftaroline (zone diameter changed from IP to "-").
New comment: Comment 4.
Control of the inhibitor
New table.
component of inhibitor- Strains added: K. pneumoniae ATCC 700603 and S. aureus ATCC
combination disks
29213.
QC ranges added: Ceftolozane-tazobactam (MIC and zone diameter).
Quality control strains for New format.
detection of resistance
QC ranges added: Streptomycin (target susceptibility) with E. faecalis
mechanisms
ATCC 51299.
ATCC 29213
Routine QC
Routine quality control
Routine QC

(NCTC 12241, CIP 76.24, DSM 1103, CCUG 17620, CECT 434)
Disk diffusion methodology: Mueller-Hinton agar, McFarland 0.5, air, 351C, 182h. Read zone edges as the point showing
no growth viewed from the back of the plate against a dark background illuminated with reflected light.
MIC
(mg/L)
Antimicrobial agent
Amikacin
Amoxicillin
Amoxicillin-clavulanic acid4,5
Ampicillin
Ampicillin-sulbactam5,7
Aztreonam
Cefadroxil
Cefalexin
Cefepime
Cefixime
Cefotaxime
Cefoxitin
Cefpodoxime
Ceftaroline
Ceftazidime
Ceftibuten
Ceftobiprole
Ceftolozane-tazobactam8,9
Ceftriaxone
Cefuroxime
Chloramphenicol
Ciprofloxacin
Colistin
Doripenem
Ertapenem
Fosfomycin10
Gentamicin
Imipenem
Levofloxacin
Mecillinam
Meropenem
Moxifloxacin
Nalidixic acid
Netilmicin
Nitrofurantoin
Norfloxacin
Ofloxacin
Pefloxacin
Piperacillin
Piperacillin-tazobactam5,8
Ticarcillin
Ticarcillin-clavulanic acid4,5
Tigecycline11
Tobramycin
Trimethoprim
Trimethoprim-sulfamethoxazole12
Target1
1-2
4
4
4
2
0.125
8
0.03-0.06
0.5
0.06
4
0.5
0.06
0.125-0.25
0.25
0.06
0.25
0.06
4
4
0.008
0.5-1
0.03
0.008
1
0.5
0.125
0.016-0.03
0.06-0.125
0.016-0.03
0.016-0.03
2
8
0.06
0.03-0.06
2
2
8
8
0.125
0.5
1
0.52
Range2
0.5-4
2-8
2-8
2-8
1-4
0.06-0.25
4-16
0.016-0.125
0.25-1
0.03-0.125
2-8
0.25-1
0.03-0.1253
0.06-0.5
0.125-0.5
0.03-0.1253
0.125-0.53
0.03-0.125
2-8
2-8
0.004-0.016
0.25-2
0.016-0.06
0.004-0.016
0.5-23
0.25-1
0.06-0.25
0.008-0.06
0.03-0.25
0.008-0.06
0.008-0.06
1-4
0.5-1
4-16
0.03-0.125
0.016-0.125
1-4
1-4
4-16
4-16
0.03-0.25
0.25-1
0.5-2
-
Disk content
(g)
30
20-10
10
10-10
30
30
30
30
5
5
30
10
5
10
30
5
30-10
30
30
30
5
10
10
10
10
5
10
10
5
30
10
100
10
5
5
30
30-6
75
75-10
15
10
5
1.25-23.75
Inhibition zone diameter

(mm)
Target1
Range3
22-23
19-26
21
18-246
18-19
15-226
21-22
19-246
32
28-36
17
14-20
18
15-21
34
31-37
25
23-27
28
25-31
26
23-29
25-26
23-28
27
24-30
26
23-29
31
27-35
28
25-31
28
24-32
32
29-35
23
20-26
24
21-27
35
30-40
31
27-35
32-33
29-36
22-23
19-26
29
26-32
33
29-37
27
24-30
31-32
28-35
31-32
28-35
25
22-28
21
18-24
20
17-23
31-32
28-35
31
29-33
29
26-32
24
21-27
24
21-27
27
24-30
27
24-30
23-24
20-27
22
18-26
24-25
21-28
26
23-29
Routine QC

1
Calculated by EUCAST.
From International Standards Organisation, ISO 20776-1: 2006 (with updates as in the latest CLSI M100 document), except
ranges in bold/italics established by EUCAST.
3
From Clinical and Laboratory Standards Institute, M100-S26, 2016, except ranges in bold/italics established by EUCAST. All
ranges have been validated by EUCAST.
4
For MIC testing, the concentration of clavulanic acid is fixed at 2 mg/L.
5
E. coli ATCC 35218 (TEM-1 -lactamase-producing strain) is used to check the inhibitor component (see Routine quality
control for -lactam--lactamase inhibitor combination disks).
6
Ignore growth that may appear as a thin inner zone on some batches of Mueller-Hinton agar.
7
For MIC testing, the concentration of sulbactam is fixed at 4 mg/L.
8
For MIC testing, the concentration of tazobactam is fixed at 4 mg/L.
9
Either E. coli ATCC 35218 or K. pneumoniae ATCC 700603 can be used to check the inhibitor component (see Routine
quality control for -lactam--lactamase inhibitor combination disks).
10
Fosfomycin MICs must be determined in the presence of glucose-6-phosphate (25 mg/L in the medium for broth and agar
dilution methods). Follow the manufacturer's instructions for commercial systems.
11
For tigecycline broth microdilution MIC determination, the medium must be prepared fresh on the day of use.
12
Trimethoprim:sulfamethoxazole in the ratio 1:19. MIC values are expressed as the trimethoprim concentration.
2
Routine QC
Pseudomonas aeruginosa ATCC 27853

MIC
(mg/L)
Antimicrobial agent
Amikacin
Aztreonam
Cefepime
Ceftazidime
Ciprofloxacin
Colistin
Doripenem
Fosfomycin6
Gentamicin
Imipenem
Levofloxacin
Meropenem
Netilmicin
Piperacillin
Piperacillin-tazobactam4,7
Ticarcillin
Ticarcillin-clavulanic acid7,8
Tobramycin
Target1
2
4
1-2
2
0.5
0.5
1-2
0.25
4
1
2
1-2
0.5
2
2-4
2-4
16
16
0.5
Range2
1-4
2-8
0.5-4
1-4
0.25-13
0.25-1
0.5-4
0.125-0.5
2-83
0.5-2
1-4
0.5-4
0.25-1
0.5-8
1-8
1-8
8-32
8-32
0.25-1
Disk content
(g)
30
30
30
10
30-10
5
10
10
10
5
10
10
30-6
75-10
10

(mm)
Target1
Range3
22
18-26
26
23-29
28
25-31
24
21-27
28
25-31
29
25-33
31-32
28-35
20
17-23
24
20-28
22-23
19-26
30
27-33
18
15-21
26
23-29
24
20-28
23
20-26
From International Standards Organisation, ISO 20776-1: 2006 (with updates as in the latest CLSI M100 document).
3
4
5
Either E. coli ATCC 35218 or K. pneumoniae ATCC 700603 can be used to check the inhibitor component (see Routine
quality control for -lactam--lactamase inhibitor combination disks).
6
7
E. coli ATCC 35218 (TEM-1 -lactamase-producing strain) is used to check the inhibitor component (see Routine quality
control for -lactam--lactamase inhibitor combination disks).
8
2
Routine QC

(NCTC 12973, CIP 103429, DSM 2569, CCUG 15915, CECT 794)
-lactamase-producing strain (weak)
MIC
(mg/L)
Antimicrobial agent
Amikacin
Ampicillin
Azithromycin
Benzylpenicillin
Cefoxitin
Ceftaroline
Ceftobiprole
Chloramphenicol
Ciprofloxacin
Clarithromycin
Clindamycin
Dalbavancin5
Daptomycin6
Doxycycline
Erythromycin
Fosfomycin7
Fusidic acid
Gentamicin
Levofloxacin
Linezolid
Minocycline
Moxifloxacin
Mupirocin
Netilmicin
Nitrofurantoin
Norfloxacin
Ofloxacin
Oritavancin5
Quinupristin-dalfopristin
Rifampicin
Tedizolid
Teicoplanin
Telavancin5
Tetracycline
Tigecycline8
Tobramycin
Trimethoprim
Vancomycin
Target1
2
1
0.5-1
2
0.25
0.25-0.5
4-8
0.25
0.25
0.125
0.06
0.25-0.5
0.25
0.5
1-2
0.125
0.25-0.5
0.125-0.25
2
0.125-0.25
0.03-0.06
0.125
0.252
16
1
0.25-0.5
0.03-0.06
0.5
0.008
0.5
0.5
0.06
0.25-0.5
0.06-0.125
0.25-0.5
2
0.52
1
Range2
1-4
0.5-2
0.25-2
1-4
0.125-0.54
0.125-14
2-16
0.125-0.5
0.125-0.5
0.06-0.25
0.03-0.1254
0.125-1
0.125-0.5
0.25-1
0.5-44
0.06-0.25
0.125-1
0.06-0.5
1-4
0.06-0.5
0.016-0.125
0.06-0.25
8-32
0.5-2
0.125-1
0.016-0.1254
0.25-1
0.004-0.016
0.25-14
0.25-1
0.03-0.125
0.125-1
0.03-0.25
0.125-1
1-4
0.5-2
Disk content
(g)
30
2
1 unit
30
5
5
30
5
2
15
10
10
5
10
30
5
200
10
100
10
5
15
5
30
15
10
5
1.25-23.75
-

(mm)
1
Target
Range3
21
18-24
18
15-21
15
12-18
27
24-30
27
24-30
25
22-28
24
20-28
24
21-27
26
23-29
26
23-29
29
26-32
22
19-25
26
23-29
24
21-27
26
23-29
28
25-31
34
31-37
23
20-26
20
17-23
21
18-24
24
21-27
24
21-27
33
30-36
27
23-31
22
19-25
23
20-26
25
22-28
29
26-32
-
Routine QC

1
3
Established and validated by EUCAST.
4
From Clinical and Laboratory Standards Institute, M100-S26, 2016, and validated by EUCAST.
2
MICs must be determined in the presence of polysorbate-80 (0.002% in the medium for broth dilution methods; agar dilution
methods have not been validated). Follow the manufacturer's instructions for commercial systems.
6
Daptomycin MICs must be determined in the presence of Ca2+ (50 mg/L in the medium for broth dilution methods; agar
dilution methods have not been validated). Follow the manufacturer's instructions for commercial systems.
7
8
9
Routine QC

MIC
(mg/L)
Antimicrobial agent
Ampicillin
Ciprofloxacin
Gentamicin
Imipenem
Levofloxacin
Linezolid
Nitrofurantoin
Norfloxacin
Quinupristin-dalfopristin
Streptomycin
Teicoplanin
Tigecycline7
Trimethoprim
Vancomycin
Target1
1
0.5-1
8
1
0.5-1
2
8
4
4
Note5
0.5
0.06
0.25
2
0.5
2
Range2
0.5-2
0.25-2
4-16
0.5-2
0.25-2
1-4
4-16
2-8
2-8
Note5
0.25-1
0.03-0.125
0.125-0.58
1-4
Disk content
(g)
2
5
304
10
5
10
100
10
15
3006
30
15
5
1.25-23.75
5

(mm)
Target1
Range3
18
15-21
22
19-25
15
12-18
27
24-30
22
19-25
22
19-25
21
18-24
19
16-22
14
11-17
17
14-208
18
15-21
23
20-26
28
24-32
30
26-34
13
10-16
3
4
Screening disk for high-level aminoglycoside-resistance in enterococci.
5
There is currently no MIC range for E. faecalis ATCC 29212 and streptomycin 300 g.
6
Screening disk for high-level streptomycin-resistance in enterococci.
7
8
From Clinical and Laboratory Standards Institute, M100-S26, 2016.
9
2
Routine QC
Streptococcus pneumoniae ATCC 49619*

(NCTC 12977, CIP 104340, DSM 11967, CCUG 33638)
Strain with reduced susceptibility to benzylpenicillin
* Zone edges for S. pneumoniae on MH-F are often accompanied by -haemolysis. Read inhibition of growth and not
inhibition of haemolysis. Tilt the plate to easier differentiate between haemolysis and growth. There is usually growth in the
whole area of -haemolysis but on some MH-F media, there is additional -haemolysis without growth.
Disk diffusion methodology: Mueller-Hinton agar + 5% defibrinated horse blood and 20 mg/L -NAD, McFarland 0.5, 5%
CO2, 351C, 182h. Read zone edges as the point showing no growth viewed from the front of the plate with the lid
removed and with reflected light.
Antimicrobial agent
Amoxicillin
Ampicillin
Azithromycin
Benzylpenicillin
Cefaclor
Cefepime
Cefotaxime
Cefpodoxime
Ceftaroline
Ceftobiprole
Ceftriaxone
Cefuroxime
Chloramphenicol
Ciprofloxacin
Clarithromycin
Clindamycin
Dalbavancin5
Daptomycin6
Doripenem
Doxycycline
Ertapenem
Erythromycin
Imipenem
Levofloxacin
Linezolid
Meropenem
Minocycline
Moxifloxacin
Nitrofurantoin
Norfloxacin
Ofloxacin
Oritavancin5
Oxacillin7
Rifampicin
Tedizolid
Teicoplanin
Telithromycin
Tetracycline
Tigecycline8
Vancomycin
MIC
(mg/L)
Target1
0.06
0.125
0.125
0.5
2
0.06-0.125
0.06
0.06
0.016
0.016
0.06
0.5
4
0.06
0.06
0.016
0.125-0.25
0.06
0.03-0.06
0.06-0.125
0.06
0.06
1
0.5-1
0.125
0.125
8
4
2
0.002
0.03
0.25
0.008-0.016
0.125-0.25
0.03-0.06
0.25-0.5
0.25
Range2
0.03-0.125
0.06-0.25
0.06-0.25
0.25-1
1-4
0.03-0.25
0.03-0.125
0.03-0.125
0.008-0.034
0.004-0.034
0.03-0.125
0.25-1
2-8
0.03-0.125
0.03-0.125
0.008-0.034
0.06-0.5
0.03-0.125
0.016-0.125
0.03-0.25
0.03-0.125
0.03-0.125
0.5-2
0.25-2
0.06-0.25
0.06-0.25
4-16
2-8
1-4
0.001-0.0044
0.016-0.06
0.125-0.54
0.004-0.03
0.06-0.5
0.016-0.125
0.125-1
0.125-0.5
Disk content
(g)
2
1 unit
30
30
5
10
30
30
30
5
2
10
10
15
10
5
10
10
30
5
100
10
5
1
5
30
15
30
15
1.25-23.75
5

(mm)
1
Target
Range3
28
25-31
19
16-22
28
25-31
34
31-37
31
28-34
32
29-35
35
32-38
31
28-34
27
24-30
25
22-28
25
22-28
34
31-37
31
28-34
29
26-32
38
34-42
24
21-27
26
23-29
34
30-38
28
25-31
27
24-30
28
25-31
21
18-24
21
18-24
11
8-14 7
29
26-32
21
18-24
30
27-33
31
28-34
27
24-30
22
18-26
20
17-23
10
Routine QC
Streptococcus pneumoniae ATCC 49619*

(NCTC 12977, CIP 104340, DSM 11967, CCUG 33638)
Strain with reduced susceptibility to benzylpenicillin
1
3
4
2
MICs must be determined in the presence of polysorbate-80 (0.002% in the medium for broth dilution methods; agar
dilution methods have not been validated). Follow the manufacturer's' instructions for commercial systems.
6
Daptomycin MICs must be determined in the presence of Ca2+ (50 mg/L in the medium for broth dilution methods; agar
dilution methods have not been validated). Follow the manufacturer's instructions for commercial systems.
7
S. aureus ATCC 29213 can be used for quality control of oxacillin 1 g with target 22 mm and range 19-25 mm
(according to disk diffusion methodology for S. aureus ).
8
9
11
Routine QC

(NCTC 12975, CIP 103570, DSM 11970, CCUG 29539)
CO2, 351C, 182h. Read zone edges as the point showing no growth viewed from the front of the plate with the lid removed
and with reflected light.
MIC
(mg/L)
Antimicrobial agent
3,4
Amoxicillin-clavulanic acid
Amoxicillin
Ampicillin
Ampicillin-sulbactam5
Azithromycin
Benzylpenicillin
Cefepime
Cefixime
Cefotaxime
Cefpodoxime
Ceftaroline
Ceftibuten
Ceftriaxone
Cefuroxime
Chloramphenicol
Ciprofloxacin
Clarithromycin
Doripenem
Doxycyline
Ertapenem
Erythromycin
Imipenem
Levofloxacin
Meropenem
Minocycline
Moxifloxacin
Nalidixic acid
Ofloxacin
Rifampicin
Roxithromycin
Telithromycin
Tetracycline
Target1
0.25
0.25
0.125
0.125
1
0.06
0.03
0.008
0.06
0.008
0.03
0.004
0.5
0.5
0.008
8
0.125
0.5
0.03
4
0.5
0.016
0.06
0.25
0.016
0.03
0.5
8
2
0.5
0.03
Range2
0.125-0.5
0.125-0.5
0.06-0.25
0.06-0.25
0.5-2
0.03-0.125
0.016-0.06
0.004-0.016
0.03-0.125
0.004-0.016
0.016-0.06
0.002-0.008
0.25-16
0.25-1
0.004-0.016
4-16
0.06-0.256
0.25-1
0.016-0.066
2-8
0.25-16
0.008-0.03
0.03-0.1256
0.125-0.5
0.008-0.03
0.016-0.06
0.25-1
4-16
1-4
0.25-1
0.016-0.06
Disk content
(g)
2-1
2
1 unit
30
5
5
10
30
30
30
30
5
10
10
15
10
5
10
30
5
30
5
5
15
30
1.25-23.75

(mm)
Target
Range
20
17-23
22
19-25
18
15-21
33
30-36
32
29-35
33
29-37
33
30-36
34
31-37
38
34-42
30
26-34
34
31-37
36
32-40
29
26-32
30
27-33
13
10-16
27
24-30
35
31-39
31
27-35
29
26-32
33
30-36
30
27-33
34
31-37
24
21-27
17
14-20
31
28-34
31
27-35
3
4
S. aureus ATCC 29213 (-lactamase-producing strain) is used to check the inhibitor component (see Routine quality control
for -lactam--lactamase inhibitor combination disks).
5
6
7
2
12
Routine QC
Campylobacter jejuni ATCC 33560

(NCTC 11351, CIP 702, DSM 4688, CCUG 11284)
Disk diffusion methodology: Mueller-Hinton agar + 5% defibrinated horse blood and 20 mg/L -NAD, McFarland 0.5,
microaerobic environment, 411C, 24h. Read zone edges as the point showing no growth viewed from the front of the plate
with the lid removed and with reflected light. The MH-F plates should be dried prior to inoculation to reduce swarming (at 2025C over night or at 35C, with the lid removed, for 15 min).
MIC
(mg/L)
Antimicrobial agent
Ciprofloxacin
Erythromycin
Tetracycline
Target
IP
IP
IP
Range
IP
IP
IP
Disk content
(g)
5
15
30

(mm)
Target1
Range2
38
34-42
31
27-35
34
30-38
IP = In Preparation
13
Routine QC
Control of the inhibitor component of -lactam--lactamase inhibitor combination

disks

TEM-1 -lactamase-producing strain (non-ESBL) used to check the inhibitor component of -lactam--lactamase inhibitor
combination disks.
MIC
(mg/L)
Antimicrobial agent
3
Ampicillin-sulbactam5
Piperacillin-tazobactam6
Ticarcillin-clavulanic acid3
Target1
8-16
32-64
0.125
1
16
Range2
4-32
16-128
0.06-0.25
0.5-2
8-32
Disk content
(g)
20-10
10-10
30-10
30-6
75-10

(mm)
Target1
Range2
19-20
17-224
16
13-194
28
25-31
24
21-27
23
21-25
Klebsiella pneumoniae ATCC 700603

(NCTC 13368, CCUG 45421, CECT 7787)
SHV-18 ESBL producer
MIC
(mg/L)
Antimicrobial agent
Ceftolozane-tazobactam
6,7
Target1
1
Range2
0.5-2
Disk content
(g)
30-10

(mm)
Target1
Range2
21
17-25

MIC
(mg/L)
Antimicrobial agent
Target1
Note8
Range2
Note8
Disk content
(g)
2-1

(mm)
Target1
Range2
22
19-25
3
4
Ignore growth that may appear as a thin inner zone on some batches of Mueller-Hinton agar.
5
6
7
Either E. coli ATCC 35218 or K. pneumoniae ATCC 700603 can be used to check the inhibitor component.
8
E. coli ATCC 35218 is used to check the inhibitor component.
2
14
Extended QC
Extended quality control for detection of

resistance mechanisms with disk diffusion
15
Extended QC
Quality control strains for detection of resistance mechanisms with disk diffusion
on Mueller-Hinton agar
Disk diffusion methodology: Mueller-Hinton agar, McFarland 0.5, air, 351C, 182h. Read zone edges as the point
showing no growth viewed from the back of the plate against a dark background illuminated with reflected light.
ESBL production in Enterobacteriaceae

Klebsiella pneumoniae ATCC 700603
(NCTC 13368, CCUG 45421, CECT 7787)
SHV-18 ESBL-producer
Antimicrobial agent
Aztreonam
Cefotaxime
Cefpodoxime
Ceftazidime
Ceftriaxone
Disk content
(g)
30
5
10
10
30
Target
susceptibility1
R
I or R
R
I or R
I or R
Range2
(mm)
9-17
12-18
9-16
6-12
16-22
Comments
Methicillin resistance in Staphylococcus aureus

Staphylococcus aureus NCTC 12493
(CCUG 67181)
Methicillin resistant (MRSA), mecA positive
Antimicrobial agent
Cefoxitin
Disk content
(g)
30
Target
susceptibility1
R
Range2
(mm)
14-20
Comments
VanB -mediated glycopeptide resistance in enterococci

(NCTC 13379 ,CIP 104676, DSM 12956, CCUG 34289)
vanB -positive strain
Antimicrobial agent
Teicoplanin
Vancomycin
Disk content
(g)
30
5
Target
susceptibility1
S
R
Range2
(mm)
16-20
6-12
Comments
Examine zone edge with transmitted light (plate
held up to light). Inhibition zones with fuzzy zone
edges are interpreted as resistant, even if the
zone diameter is above the susceptible
breakpoint.
High-level aminoglycoside resistance in enterococci

(NCTC 13379 ,CIP 104676, DSM 12956, CCUG 34289)
High-level gentamicin and streptomycin resistant
Antimicrobial agent
Gentamicin
Streptomycin
Disk content
(g)
30
300
Target
susceptibility1
R
R
Range2
(mm)
6
6
Comments
Targets comply with EUCAST clinical breakpoints and are set to ensure that resistance mechanisms are correctly detected.
Interpretation according to EUCAST clinical breakpoints: S=Susceptible, I=Intermediate, R=Resistant.
2
IP = In Preparation
16
Extended QC
Quality control strains for detection of resistance mechanisms with disk diffusion
on Mueller-Hinton fastidious (MH-F) agar
CO2, 351C, 182h. Read zone edges as the point showing no growth viewed from the front of the plate with the lid removed
and with reflected light.
Reduced susceptibility to -lactam agents due to PBP mutations in Haemophilus

influenzae
(NCTC 12699, CIP 104604, DSM 9999, CCUG 26214)
Antimicrobial agent
Disk content
(g)
Target
susceptibility1
Range2
(mm)
Comments
Inhibition zone diameters are particularly affected
by variation in medium, inoculum and incubation
conditions. Inhibition zones with growth of small
colonies within the zone are interpreted as 6 mm
(no zone).
Ampicillin
Benzylpenicillin
2
1 unit
R
R
6-12
6-9
Targets comply with EUCAST clinical breakpoints and are set to ensure that resistance mechanisms are correctly detected.
Interpretation according to EUCAST clinical breakpoints: S=Susceptible, I=Intermediate, R=Resistant.
2
Established and validated by repeated testing by EUCAST.
17

V 6.1 EUCAST QC Tables Routine and Extended QC

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European Committee on Antimicrobial

This document should be cited as

Routine quality control

Extended quality control for detection of resistance mechanisms

EUCAST QC Tables v. 6.1, valid from 2016-03-01

EUCAST QC Tables v. 6.1, valid from 2016-03-01

Changes from previous version

QC ranges added: Dalbavancin, oritavancin and tedizolid (MIC).

EUCAST QC Tables v. 6.1, valid from 2016-03-01

Routine quality control

EUCAST QC Tables v. 6.1, valid from 2016-03-01

Escherichia coli ATCC 25922

Inhibition zone diameter

EUCAST QC Tables v. 6.1, valid from 2016-03-01

Escherichia coli ATCC 25922

EUCAST QC Tables v. 6.1, valid from 2016-03-01

Pseudomonas aeruginosa ATCC 27853

Inhibition zone diameter

EUCAST QC Tables v. 6.1, valid from 2016-03-01

Staphylococcus aureus ATCC 29213

Inhibition zone diameter

EUCAST QC Tables v. 6.1, valid from 2016-03-01

Staphylococcus aureus ATCC 29213

EUCAST QC Tables v. 6.1, valid from 2016-03-01

Enterococcus faecalis ATCC 29212

Inhibition zone diameter

EUCAST QC Tables v. 6.1, valid from 2016-03-01

Streptococcus pneumoniae ATCC 49619*

Inhibition zone diameter

EUCAST QC Tables v. 6.1, valid from 2016-03-01

Streptococcus pneumoniae ATCC 49619*

EUCAST QC Tables v. 6.1, valid from 2016-03-01

Haemophilus influenzae ATCC 49766

Inhibition zone diameter

EUCAST QC Tables v. 6.1, valid from 2016-03-01

Campylobacter jejuni ATCC 33560

Inhibition zone diameter

EUCAST QC Tables v. 6.1, valid from 2016-03-01

Control of the inhibitor component of -lactam--lactamase inhibitor combination

Escherichia coli ATCC 35218

Inhibition zone diameter

Klebsiella pneumoniae ATCC 700603

Inhibition zone diameter

Staphylococcus aureus ATCC 29213

Inhibition zone diameter

EUCAST QC Tables v. 6.1, valid from 2016-03-01

Extended quality control for detection of

EUCAST QC Tables v. 6.1, valid from 2016-03-01

ESBL production in Enterobacteriaceae

Methicillin resistance in Staphylococcus aureus

VanB -mediated glycopeptide resistance in enterococci

High-level aminoglycoside resistance in enterococci

EUCAST QC Tables v. 6.1, valid from 2016-03-01

Reduced susceptibility to -lactam agents due to PBP mutations in Haemophilus

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