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MODULE CODE
: 'BENG3009
BENG3009A
MODULE NAME
DATE
09-May-11
TIME
10:00
TIME ALLOWED
2 Hours 0 Minutes
ASSESSMENT
PATTERN
2010/11-BENG3009A-001-EXAM-39
2010 University College London
TURN OVER
Answer ALL FOUR QUESTIONS. Marks for each question are distributed as
shown in [J.
1.
G(s)
30s +1
[30]
2.
A biotech company has two production processes, one each for drug A
and drug B. The batch time for drug A and drug B are 11 and 8 days
respectively. The gaps between two consecutive batches are 5 days for
drug A and 4 days for drug B. The relationship between operating time
and batch number for a process is as follows:
Due to staff constraints, only one process can be operated at any time.
There are 230 working days available in a year. The profits for one
batch of drug A and B are 100,000 and 60,000 respectively. However
the maximum demands for these drugs are 40 batches for drug A and 30
batches for drug B.
a)
b)
[15]
[15]
BENG3009
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2010/11
3.
b)
Write a function for calculating the level of protein release, R, given the
pressure, P, and the number of passes N.
Write a function for the grade efficiency curve T(d) of the clarifying
centrifuge at a given flowrate, Q.
c)
[10]
[10]
[10]
Background information:
Ln
=K.N.Pa
(1)
where Kp = 4.14X10-8,
d
T(d)=1 exp(-1C,(
)2)
d
(2)
AlApE(1 COrg
(3)
is 1633
CONTINUED
BENG3009
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2010/11
4.
[10]
Page 4 of 5
2010/11
Comparison Analysis
w
Process Definition
O
O
Sizing Basis
Expression (# particles or g/L)
Target Capacity Utilisation
Production Bloreactor Working Volume
Total Number of Production Bioreactors
Production / Seed Train Split
Number of Bioreactors! Batch
Traditional
Single-Use
Microb Typical
Microb Typical
Gram
2.0E+00
90%
1000 L
Gram
2.0E+00
90%
1000 L
3
2
per unit op
per unit op
per unit op
per unit op
No
No
No
No
No
Yes
Yes
Yes
Liner
Yes
Yes
Yes
3000
GON!
W34i1 *
Consumables
Labour
F,c)Wi
Solution Preparation
Media Make-up Basis
Buffer Make-up Basis
Make up Concentrated Buffers
TOTAL
12 88
9 53
4.07
23 53
4 23
54 24
64.38
47 66
20 35
117.64
21 16
271 19
29,898
22,135
9,449
54,632
9,826
125,940
12,646,870
9,363,048
3,996,976
23,109,449
4,156,320
53,272,663
8%
100%
3.91
19 54
43 87
76 32
60 55
5 33
205.60
9,072
20,371
35,446
28,120
2,474
95,483
3,837,609
8,617,075
14,993,454
11,894,908
1,046,409
40,389,455
10%
21%
37%
29%
3%
100%
frtl
tm
Lh
ro
it
t
LI
Liner
No
No
No
3000
No
10
U'
8 77
15 26
10#
Consumables
Labour
12 11
1.07
000gr
TOTAL
41 12
Gthana
No
10
29
48.4
31.4
Comparison
Annual CoG (US$)
Capital
Materials
Consumables
Labour
Other
Traditional
53,272,663
12,646,870
9,363,048
3,996,976
23,109,449
4,156,320
8%
43%
Single-Use Systems
Bloreactors (up to 2000L)
Media Preparation (up to 2000L)
Buffer Preparation (up to 2000L)
Type of Buffer Prep System
Media and Buffer Hold (up to 3000L)
intermediate Product Hold (up to 2000L;
Use Stainless Steel above Threshold
Threshold Volume
Membrane Adsorber for Flowthrough
Size of MA Capsule
24%
18%
Single-Use % Change
40,389,455
-24%
3,837,609
-70%
8,617,075
-8%
14,993,454
275%
11,894,908
-49%
1,046,409
-75%
i6
29
146.8
Vessels
Bags
GGiargkim
2
0
rifl
12
0
12
0
9
0
Other metrics
51.34
31 36
60.39
423
196 44
4.53
146.80
18.32
-91%
368%
-70%
423
196 44
0%
0%
Vessels
Bags
0
2
0
12
0
12
1akcet4
0
9