INDIAN COUNCIL OF AGRICULTURAL RESEARCH

RESEARCH PROJECT PROFORMA FOR MONITORING ANNUAL PROGRESS (RPP- II)

(Refer for Guidelines ANNEXURE-XI (E))
1.
2.
3.
4.

Institute Project Code ............................................................................................

Project Title :
Evaluation of locally available plants for feeding of hill animals
Reporting Period
:
August, 2014-March, 2015.
Project Duration: Date of Start - August, 2012
Likely Date of Completion July, 2016 (Requested for 1yr

5.

extension)
Project Team (Name(s) and designation of PI, CC-PI and all project Co-PIs, (with time spent for the project) if
any additions/deletions
S.
No.

Name, designation and

institute

Status in
the project
(PI/CC-PI/
Co-PI)

Time to
be spent
(%)

Work components assigned to individual

scientist

1.

Dr. R. Bhar;
Scientist,
IVRI
Station, Palampur

PI

40

Planning, management of experimental

animals, In-vivo animal trials, data
interpretation and analysis.

2.

Dr. A. Kannan, Sr. Scientist,

IVRI
Regional
Station,
Palampur

Co-PI

40

In-vitro trial, biological sample analysis,

compilation of data, and report writing.

3.

Dr. B. Sahoo, Sr. Scientist

(Animal Nutrition), IVRI
Regional Station, Mukteswar,
Uttarakhand (w.e.f., January,
2014)

Co-PI

40

Studies on seasonal variation in the

content of different nutrients in locally
available plants/leaves.
Nutritional trials for development of
alternate diets on incorporating such
locally available plants at different
levels.
Collection of samples at Uttarakhand for
estimation of plant secondary metabolites
at IVRI Regional Station, Palampur, H.P.

4.

Dr. Rinku Sharma, Scientist,

IVRI
Regional
Station,
Palampur

Co-PI

20

Clinical nutritional aspect; blood

biochemical parameters and faecal egg
count.

5.

Dr Gorakh Mal, Pr. Scientist,

IVRI
Regional
Station,
Palampur

Co-PI

40

Identification and quantification of

phytochemicals in local plants/leaves/
grasses viz., oxalates and flavonoids;
review of the literature & publication of
data.

6.

Dr. S. Radotra, Scientist

Incharge, IGFRI Regional
Station, Palampur, H.P.

Co-PI

30

Cultivation of fodders and goat

production and management at IGFRI.

7.

Dr. K. B. Sharma, Prof., Dept

of
Physiology,
H.P.K.V,
Palampur

Co-PI

20

Mineral nutrition/analysis.

Principal
Regional

6.

(a) Activities and outputs earmarked for the year (as per activities schedule given in RPP-I)
Obtive
wise

1.

Activity

Scientist
responsible

%
of
envisaged
completed
RPP-I

100

100

100

Studies on oxalate content Dr Gorakh Mal 100

in oak leaves and
different grasses
Studies on flavonoids profiles of Dr Gorakh 100
different tree leaves and fruits
Mal
Effect of different chemical Dr. R. Bhar 100
treatments on different polyphenol
content of Q. leucotrichophora
leaves.

100

Studies on seasonal variation in the

content of different nutrients in
locally available plants/leaves.

100

100

B.

100

100

A.

100

100

C
C

b.
Extraction
of
condensed tannins from
C.
australis,
R.
pseudoacacia
and
Bauhinia variegate for in
vitro studies

C
C

Dr. B. Sahoo

the effect of Dr K.
processing on mineral (Ca & Sharma
P) composition of oak leaves
Studies

2.

% achieved
as targeted

100

Analysis of available Dr. R. Bhar

feeds
and
fodders
(Quercus
leucotrichophora leaves,
Oat green fodders and
concentrate mixture) for
different nutrients and
phytochemicals
(phenolics).
a. Analysis of Robinia
(Robinia pseudoacacia), Dr. A. Kannan
Arjun
(Terminalia
arjuna) Khirak (Celtis
australis)
and
biul
(Grewia optiva) leaves
for
different
phytochemicals
and
nutrients for in vitro
studies.

activity
to
be
as per

on

a. In vitro studies on finding

optimum level of mixing Dr.
2

100
100

tanniferous tree leaves, Robinia Kannan

(Robinia pseudoacacia), Arjun
(Terminalia arjuna) Khirak
(Celtis australis)
with biul
leaves for feeding to ruminants
b. In vitro studies on addition of
condensed tannins extracted
from different tree leaves on
rumen fermentation in vitro
Preparation of land and Dr.S. Radotra 100
cultivation of oat green fodder
and brachiaria grasses for the
next trial
Studies on development of Dr. R. Bhar 100
concentrate supplemented oak
(Q. leucotrichophora) leaves
based diets for Gaddi goats (in
vivo trial).
Dr
Rinku 100
Clinical-pathological
examination of faeces in Gaddi Sharma
goats
fed
on
Q.
leucotrichophora (oak) leaves.

100

100

100

(b) If shortfall/addition, reasons for the same and how to catch up with the intended activities
7.

Annual Progress Report (research results and achievements in bullets)

(I) Comparative Studies on Some Phyto-chemicals Content in the

Oak Leaves of Palampur & Mukteswar:
Oxalate Contents:
Monthly variability of the Oxalate contents of Q. leucotrichophora leaves of
Palampur and Mukteswar origin was studied. Monthly variability of Q. semicarpifolia
was also studied. The ranges of oxalate contents of Palampur and Mukteswar
originated Q. leucotrichophora leaves was 1.950.03 to 3.460.03% and 1.570.08
to 3.310.06% (on DM basis), and of Mukteswar originated Q. semicarpifolia leaves
was 1.170.05 to 4.790.08%. The highest level was found in the month of
June in Palampur; whereas the highest level in Mukteswar was found in
April in Q. leucotrichophora leaves and in November in Q. semicarpifolia leaves.
The lowest level was found in the month of January in Palampur; whereas
the lowest level in Mukteswar was found in September in Q.
leucotrichophora leaves and in June in Q. semicarpifolia leaves.
Oxalate contents of sixteen other locally available tree leaves were also studied.
Among the tree leaves highest level (5.900.03%) was found in Albizia chinensis
3

(Oie) leaves; and lowest level (1.070.03) in Trifolium repens (White clover) leaves.
In the seed of Camellia sinensis (Tea) it was further lower, i.e., 0.880.05%, only.
Antioxidant activities and Flavonoides contents:
Antioxidant activities of Q. leucotrichophora leaves of Palampur and Mukteswar origin and of Q.
semicarpifolia leaves of Mukteswar origin was studied on following ABTS and DPPH assay. It was
observed that the antioxidant activities of Q. semicarpifolia leave of Mukteswar origin was more than
that of Q. leucotrichophora leaves of Palampur. The IC50 of Q. semicarpifolia leave was
5.830.10 g/ml, on ABTS assay and 7.670.13 g/ml, on DPPH assay; it was
5.470.06 and 6.330.11 g/ml in assay in Mukteswar originated Q. leucotrichophora
leaves. Whereas, it was lowest to the extent 4.430.11 g/ml, on ABTS assay and
5.690.03 g/ml, on DPPH assay in Palampur originated Q. leucotrichophora leaves.
Similarly the flavonoides content of these leaves were found to be maximum (i.e., 23.350.42 mg/g
Quercetin equivalent) in Mukteswar originated Q. semicarpifolia leaves (Kharsu), followed
by Mukteswar originated Q. leucotrichophora leaves (Banj) (18.930.60 mg/g) and was
lowest (16.800.25 mg/g) in Palampur originated Q. leucotrichophora (Bun) leaves.
Antioxidant activity by qualitative DPPH test on F254 TLC plate was also done in
some other regionally available plant samples (viz., Tea seed; Tremal, Arjun, Sea
buckthorn, Kachnar, jungli Khajur, Khirk and Oie leaves) (Fig. 1-2).
Fig1. Antioxidant activity by qualitative DPPH test on F254 TLC plate
1
9

10

11

Lanes 1+2: Ascorbic acid (+ve control; 5l & 10l); Lanes 3+4: Oak (Bun;
Palampur)- 5l & 10l; Lanes 5+6: Oak (Banj; Mukteswar)- 5l & 10l;
Lanes 7+8: Oak (Kharsu; Mukteswar) - 5l & 10l; Lanes 9+10: Tea seed
(5l & 10l); Lane 11: Methanol (-ve control) - 10l

Fig2. Antioxidant activity by qualitative DPPH test on F254 TLC plate

1
7

2
8

Lane 1: Methanol (-ve control); Lane 2: Ascorbic acid (+ve control); Lane 3: Tremal;
Lane 4: Arjun; Lane 5: Sea buckthorn; Lane 6: Kachnar; Lane 7: jungli Khajur; Lane
8: Khirk & Lane 9: Oie

(II) In vitro Studies:

Effect of chopping on the polyphenol and oxalate content, antioxidant activity and
in vitro digestibility of Q. leucotrichophora leaves based diets were studied at the
laboratory level. Fresh mature oak (Q. leucotrichophora) leaves were manually
lopped from the nearby forest area of Palampur, Kangra District, H.P. Green oat
fodder were procured from the local supplier. Chemical composition (DM, OM, CP,
EE, ash and fibre fractions), polyphenol content, oxalate content and antioxidant
activity of both the oak leaves and green oat fodder were carried out. The effect of
chopping on the polyphenol content, oxalate content and antioxidant activity of
these leaves were studied. Total phenols (TP) and non-tannin phenols (NTP) were
estimated by Folin Ciocalteau method in combination with polyvinylpolypyrrolidone, with tannic acid as a reference standard (Makkar, 2003). The
condensed tannins (CT) were estimated by using butanol- HCl method (Porter et al.,
1986). Oxalate content was estimated by permanganometric method given by
Sanchez-Alonso and Lachica (1987). Antioxidant activity was assayed by DPPH
antioxidant assay. The effect on rumen fermentation were investigated by in vitro
5

gas system (Menke et al., 1979) at different Green fodder (oats) : oak leaves ratios
(100:0, 75:25, 50:50, 25:75, 0:100). Syringes were incubated at 39 C for 24 h. After
24 h, the incubation were stopped and total gas production was recorded and the
contents were analyzed for ammonia-N, methane production and protozoa number.
RESULTS IN BRIEF:
Chemical Composition and polyphenol contents:
The OM, CP, EE, NFE and CF content of the oat green fodder (OGF) was 88.4, 14.7,
3.66, 46.47 and 23.57%, respectively. The OM, CP, EE, NFE and CF content of the
fresh oak leaves (FOL) were 95.93, 10.45, 4.83, 55.21 and 25.45% and for chopped
oak leaves (COL) were 95.67, 10.87, 4.37, 56.05 and 24.39%, respectively. The
values (on DM basis) of TP, NTP, TT, CT, HT are 1.2, 0.46, 0.74, 0.01and 0.73 % in
the oat green fodder; 6.93, 0.53, 6.41, 1.28 and 5.13% in fresh oak leaves; 6.23,
0.48, 5.75, 1.19 and 4.56% in the chopped oak leaves. Chopping was effective in
reduction of poly phenol content of the oak leaves. There was 10.1, 9.4, 10.3, 7.0,
11.1% reduction in the TP, NTP,TT, CT and HT values due to chopping of the oak
leaves. There was significant difference (P<0.01) in the TP, NTP, TT, CT and HT
content between the un-chopped and chopped oak leaves.
Oxalate
content
of
the
during in vitro studies (%DM)

oat

fodder

and

oak

leaves

Oxalate content of the oat green fodder was 0.46%, fresh oak leaves was 1.90% and chopped oak
leaves was 1.97%. There was no significant difference between the chopped and un-chopped oak
leaves, but there was significant difference between oat green fodder and oak leaves.
DPPH assay

The antioxidant activity of oat fodder and oak leaves were estimated by DPPH assay. Chopping
of oak leaves significantly decreased the antioxidant activity of the leaves. The total antioxidant
activity at the concentration 200 g/ml for oat fodder was 1.75% and for fresh oak was 47.10%
which got reduced to 41.34% in chopped oak leaves.
In vitro analysis of oat green fodder and oak leaves in different
combinations
There was significant reduction (P<0.01) in the polyphenol content of the oak leaves due to
chopping. Also, there was significant difference between the antioxidant activities of the chopped
oak leaves. There was linear reduction in the net gas production, dry matter, organic matter,
ammonia nitrogen and methane production as the % inclusion of the oak leaves increased
in the substrate in IVGPT.
(III) In vivo Studies: To study the performance of adult Gaddi goats
fed on Q. leucotrichophora leaf incorporated sole roughage diets
6

To study the effect of feeding diets containing fresh and chopped Q.

leucotrichophora leaves on performance of adult Gaddi goats, 18 adult female goats
of about 40 months of age was selected for the trial. After acclimatization, the
animals were randomly divided into three groups (I, II and III) of six animals each,
following completely randomized design (CRD). The goats under control group
(Group I) were fed on chopped oat green fodder (ad libitum), whereas the goats in
experimental group II were fed fresh oak leaves (ad libitum) along with 50% of the
group I of chopped green oat fodder, and goats in group III was fed fresh chopped
oak leaves along with 50% of the group I of chopped green oat fodder. The feeding
trial was conducted for ninety days. Daily feed intake and weekly changes in the
body weight were recorded throughout the trial period. Blood biochemical changes
at the 0, 45 and 90th day of the trial, was studied. A metabolism trial was
conducted at the middle of feeding trial. Digestibility, nutritive value of the diets
and balance of various nutrients were studied.
In the second phase the DMI (g/d, % BW) from the oak was found to be more than
the oat fodder in the oak leaves group. The attainment of body weight at 90 days
was higher in goats fed on oat green fodder, followed by group II and III,
respectively. Feeding of chopped oak leaves did not have any advantage when it is fed along
with the ad lib oat green. The digestibility of the DM, OM, CP, EE and fibre fraction of
fresh and chopped oak groups was significantly reduced than the control oat
groups. However, the daily intake of various nutrients was comparable between fresh and

chopped oak leaf groups. The nutrient intake (g/d) in control group (ad lib oat) was lower
(p<0.01) than other two groups (I and II). Even though there was significant difference in
the faecal outgo of N, between the groups there was no significant difference in the
N intake and balance between the control and oak groups. There was negative Ca
balance in the control group and positive Ca balances in oak groups, but there was
no significant difference between the P balance among the three groups. There was
no significant difference between the groups in serum glucose, total protein,
albumin, globulin, A:G ratio, creatinine and bilirubin. Even though elevated levels
BUN were seen in present study but, they were well within the normal ranges.
Similar finding were seen in the serum enzyme profile, AST, ALT and ALP values
were within the normal ranges. The goats fed on ad lib oak leaves (25-35 g/d tannin
intake) along with oat green did not show any symptoms of oak toxicity. However,
on ad lib feeding of oak leaves (>70% in the diet) along with oat green did not fulfill
the energy requirement of adult female Gaddi goats, though the protein, Ca and P
requirement was found to be adequate.
(IV) Studies on feeding of Setaria and hybrid napier grasses on voluntary
feed intake, nutrient utilization and performance in local gaddi goats

Studies on effect of replacing hybrid napier grass with setaria grass on

performance of adult Gaddi goats
Fifteen adult female gaddi goats were divided into three groups of five animals each
on following CRD. Three diets as mentioned in the following are randomly
distributed to three dietary groups, Control (Group I: Conc mixture+ Hybrid napier
grass), Group II (Conc mixture + Setaria grass), Group III (Conc mixture+ grass
(Hybrid napier 50% + Setaria 50%). The intake of concentrate mixture between the
groups ranges between 281 to 292g/d, the difference being non-significant between
the groups. Oat and Setaria green was offered ad libitum in groupsI and II.
7

The initial body weight of the goats between the groups ranges between 23.08
0.87 kg (Gr.III) to 23.88 1.12kg in group II, values being comparable between the
groups. The feeding trial was run for a period of 90days. At the end of trial the goats
in respective groups attend body weight of 25.88 1.02, 24.02 1.32 and 23.74
1.03kg in Groups, I, II and III, respectively. The animals in all the group could
maintain (sustain) body weight with an increase of 2.14, 0.48 and 0.66kg,
respectively. The increase in body weight was significantly more in Group fed
Hybrid Napier (Gr.I) than that of the groups II and III; difference between the groups
II and III, were non-significant. However, the values of body weight gain in goats
were about 37.5% higher than that of groups II. The values of DM intake and
digestibility of almost all the proximate nutrients (except EE) and fibre fractions did
not differ significantly between the groups; however, the values were numerically
higher in goats fed on hybrid napier, followed by the 50:50mixture of HN and
Setaria and was lowest in goats fed on setaria green grasses. Thus, looking into the
body weight gain and relatively higher values of digestibilities of most of the
proximate principals it is concluded that the setaria grass is inferior to hybrid
napier grass for feeding adult Gaddi goats.
8.

Output During Period Under Report

a.

Special attainments/innovations

The

antioxidant

activities

of

Q.

semicarpifolia

leave

of

Mukteswar origin was more than that of Q. leucotrichophora

leaves of Palampur. Similarly the flavonoides content of these
leaves were found to be maximum in Mukteswar originated Q.
semicarpifolia

leaves

(Kharsu),

followed

by

Mukteswar

originated Q. leucotrichophora leaves (Banj) and was lowest in

Palampur originated Q. leucotrichophora (Bun) leaves.

Feeding of chopped oak leaves did not have any practical

advantage when it is fed along with the ad lib oat green, though
the palatability was found to be surprisingly more of oak

leaves than that of conventional green oat fodder.

Therapeutic diets for local hill gaddi goats developed for
prevention of oak (Q. leucotrichophora leaves) toxicity at North
West Himalayan Region (NWHR):
a) Oak leaves based oat green supplemented diet (Diet No. II;
containing 6.12% DCP and 45.25% TDN)

b) Oak leaves based oat green supplemented diet, No.III (6.126.14% DCP and 45.23-45.75% TDN), developed.

It seems the requirement of energy for Gaddi goats is less than

that of NRC/ICAR recommendation.

b.

Both the grasss, sterea and hybrid nepier were found to be of sub-maintenance
type of fodder in the last year (20113-2015); Thus, on supplementation of
concentrate mixture of about 280 to 290 g/d the gaddi female goats could
maintained the body weight. The performance of animals were found to be better
than sateria alone on supplementation of mixture (50:50) of Hybrid Napier and
Setaria green garsses. However, the supplementation of concentrate @ of
about 1.2% of BW is needed to have satisfactory performance of
maintenance with weight gain, without any adverse effect on performance
and nutrient utilization.

List of Publications (one copy each to be submitted with RPP-II)

i. Research papers:

Ajith, M.K., Bhar, R., Kannan, A., Bhat, T. K., Singh, B, and Sharma, K. B. 2014.
Detannification of oak (Quercus leucotrichophora) leaves through simple physical
treatments, Animal Nutrition and Feed Technology 14 (3): 609-615.

Kannan, A., Bhat, T. K., Bhar, R., Singh, B. Mal, G. and R. Sharma 2015. Chemical
composition, polyphenol profile and in vitro digestibility of lesser known tree leaves
and shrubs of North Western Himalayan Region, Indian Veterinary Journal, 92:20-22.

Rajkumar, K., Bhar, R., Kannan, A., Jadhav, R.V., Singh, B and
Mal, G. 2015. Effect of replacing oat fodder with fresh and
chopped oak leaves on in vitro rumen fermentation,
digestibility and metabolisable energy. Veterinary World, 8(8):
1021-1026. (NAAS rating 5.1).

ii. Reports/Manuals:

Annual report of the research work undertaken submitted.

iii. Working and Concept Papers

Presentation on working & concept paper on motivational skills for

developing excellent livestock owners (under field condition) is under
preparation (Th field survey work at Bindrawan, Ghuggar and Banuri
Gram Panchyet under Palampur Thsil).
Demonstration unit at field and also at our Regional Station for Beetle
goat hard developed, in addition to the local gaddi hard at the Regional
Station.
The work is going on through field demonstration, feeding
strategies to avoid fern and oak toxicity is discussed in
gosthi. Farmers are being advised not to feed oak leaves
(Q. Leucotrichophora) ad libitum; the methods of feeding
9

of their domestic animals are being demonstrated to

avoid fern toxicity (EBH, a regional problem).
iv. Popular articles/folders:

nil

v. Books/Book Chapters:
Bhar, R., Kannan, A., Bhat, T. K., Kamra, D. N., Verma, A. K. and
Singh P. (Eds.). 2014. Tree Fodder Resources of Himachal
Pradesh, Uttarakhand and Jammu & Kashmir. Indian Veterinary
Research Institute, Regional Station, Palampur, India.
Kannan, A., Bhar, R., Radotra, S., Singh, B., Mal, G., Sharma, R.,
Sharma, V. K. and Bhat, T. K. 2014. Tree Fodder Resources of
Himachal Pradesh. In: Bhar, R., Kannan, A., Bhat, T. K., Kamra,
D. N., Verma, A. K. and Singh, P. (Eds.). 2014. Tree Fodder
Resources of Himachal Pradesh, Uttarakhand and Jammu &
Kashmir. Indian Veterinary Research Institute, Regional Station,
Palampur, India.

vi. Extension Bulletins: Scientific/teaching review on feeding of oak leaves is

under preparation.
c.

Intellectual Property Generation: Basic and applied issues/areas identified in the area till date
unexplored; necessary effort needed for collaboration /MoU with CSIR-IHBT , Palampur, H.P. for
the progress of work on the IPR issues (/generation) is being taken care off (/pursued).
(Patents - filed/obtained; Copyrights- filed/obtained; Designs- filed/obtained; Registration details
of variety/germplasm/accession if any)

d.

Presentation in Workshop/Seminars/Symposia/Conferences:
(relevant to the project in which scientists have participated)

Rajkumar, K., Bhar, R., Kannan, A., Jadhav, R.V., Bhat, T. K., Mal, G. and
Singh, B. 2014. Evaluation of feeding value of oak (Quercus leucotrichophora)
leaves as a partial substitute to oat fodder by in vitro trials. Abstract No. SLP38. Session- Sustainable livestock production in changing Climate; Global
Animal Nutrition Conference-2014 to be organised by the Animal Nutrition
Society of India and National Institute of Animal Nutrition and Physiology at
Bengaluru from 20 to 22 April, 2014.

Mal, G., Bhar, R., Singh, B., Kannan, A. and Bhat, T.K.
2014. Total oxalate and flavonoids contents in selected
plants. 5th International Conference on Advances in Food
Technology and Health Sciences (ICFTHS-2014), on 1516th October, 2014 held at JNU, New Delhi.

10

e.

Radotra, S., Bhar, R. and Kannan, A. 2014. Forage and

animal production practices in Kangra valley of Himachal
Pradesh. National Symposium on Climate Resilient Forage
Production and its Utilization, abstract page 66,
Organised by Range Management Society of India during
November 13-14 at BCKV, Kalyani, Kolkata.

Bhar, R., Kumar, N., Singh, B., Mal, G. and Saikia, P.

2015. Nutritional management of oak toxicity in hill
ruminants
with
special
reference
to Quercus
leucotrichophora leaves. Lead paper in National
Symposium on Livestock Production Practices for Small
Farms of Marginalized Groups and Communities in
India & XXII Annual Convention of Indian Society of
Animal Production and Management at COVS & AH, CAU,
Aizawl, Mizoram from 28 to 30 January, 2015.

Details of technology developed:

(Crop-based; Animal-based, including vaccines; Biological biofertilizer, biopesticide, etc; IT
based database, software; Any other please specify)

Simple dietary management for prevention of Q. leucotrichophora leaves toxicity

(on feeding ad lib) in local Gaddi goats developed.
Oak leaves based diets developed on incorporating higher level of oak leaves in
the diet of local gaddi goats.
Differences in some of the phyto-chemical (polyphenols and flavonoides)
contents between the oak leaves of Palampur and Mukteswar origin were
compaired.

i. Trainings/demonstrations organized: Initiative taken at Gram Panchayet level

ii. Any other relevant information: Unexplored area (till date) of research work initiated through
multidisciplinary team of research workers.
9.

Constraints experienced, if any: Inherent natural constraints of remote locality under regional hill environment.

10. Lessons Learnt: Mode of working in difficult/remote areas; under the constraints of limitation of basic
facilities particularly for in vivo animal trials.
11. Evaluation
(a) Self evaluation of the project for the period under report by the PI with rating
in the scale of 1 to 10
(b) Evaluation by PI on the contribution of the team in the project including self

11

9.

S.
No.

Name

Status in the project

(PI/CC-PI/Co-PI)

1.

Dr. R. Bhar

PI

2.

Dr. A. Kannan

Co-PI

3.

Dr. Rinku Sharma

Co-PI

4.

Dr Gorakh Mal

Co-PI

5.

Dr. Sahoo B.

Co-PI

6.

Dr. K. B. Sharma

Co-PI

7.

Dr. S. Radotra

Co-PI

Rating in the scale of 1 to 10

9.5
9.5
8.5
8.5
8.5
8.5
8.5

12. Signature of PI, CC-PI(s), all Co-PIs

(R. Bhar)

(A. Kannan)

(Rinku Sharma)

(Sahoo B.)

(K. B. Sharma)

(S. Radotra)

13. Signature (with specific comments on progress/achievements, shortfall and

constraints along with rating of the project in the scale of 1 to 10) of
Head of Division/Regional Center / Section

14. Comments of IRC

15. Signature (with specific comments on progress/achievements, shortfall

and constraints along with rating of the project in the scale of 1 to 10)
of JD (R)/ Director

12

(Gorakh Mal)

13