ERYTHROPOIESIS: STAGES OF MATURATION

STAGES
(NOMENCLATURE)
> Pronormoblast
>Rubriblast
>Proerythroblast

SIZE IN
MICRON
S
12-19

NUCLEUS, NC RATIO

CYTOPLASM

DIVISION

LOCATION

-round to oval containing 1 or

2 nucleoli
-chromatin: open, fine clumps
-4:1

-deeply
basophilic/blue (RNA,
ribosomes)
-no granules

-mitosis
->2 daughter
pronormoblast

-bone marrow

>Basophilic
Normoblast
> Prorubricyte
>Basophilic
erythroblast
>Polychromat(ophil)i
c normoblast
>Rubricyte
>Polychromat(ophil)i
c erythroblast

12-17

-may be present but

disappear later
-chromatin condenses
-4:1

-basophilic/deep blue
-gradually declining
RNA

-mitosis
->2 daughter
polychromatic
normoblast

-bone marrow

11-15

-varied chromatin pattern

-no nucleoli
-1:1

-many color loving:

pink, murky gray-blue
-variable color
-decreased RNA

-mitosis (last stage)

->2 orthchromic
normoblast

-bone marrow

>Orthocromic
Normoblast
>Metarubricyte
>Orthchromic
Erythocyte

8-12

-pyknotic: completely
condensed
-dark, homogenous

-pink-orange
color/acidophilic
(complete Hgb
production)
-ortho the same

-no division:
condensation of
chromatin

- bone
marrow

>Polychromat(ophil)i
c
erythrocyte
>Reticulocyte
>Diffusely basophilic
erythrocyte

7-10

-anuclear

--blue-grayish
appearance
-bluish granules: RNA
remnants, precipitated
ribosomes

-none (lack nucleus)

- bone
marrow
(1 day)
-peripheral
circulation (1
day)

>Erythrocyte

6-8

- anuclear

-biconcave disc

-none

-active in

CELLULAR ACTIVITY

LENGTH OF
TIME

-no Hgb present

-accumulate
components for Hgb
production
-globin production
begins
-Hgb synthesis (iron is
taken into cells)

-slightly more
than 24 hours

-increased Hgb
synthesis
(cytoplasm color)
-DNA transcription:
condensed nucleus,
nucleoli disappearance
-Hgb production on the
remaining ribosomes
thru mRNA
-nucleus is extruded
and engulfed by splenic
macrophages
-complete Hgb
production
-dismantled
cytoplasmic protein
production
-reduced receptor
production
-best indicator of bone
marrow function
-oxygenation-

-approximately
24 hours

-slightly more
than 24 hours

-approximately
48 hours

-2 days

-120 days

~7.5

NEUTROPHILIC GRANULE
Myeloblast

-salmon-pink or red
with central pole area
-eosinophilic

SIZE (IN MICRONS)

14-20

Promylocyte/Progranulocyt
e

16-25

Neutrophilic Myelocyte

12-18

Neutrophilic
Metamyelocyte

14-16

Neuthrophil Band

10-16

Mature / Segmented / PMN

Neutrophil

10-16

EOSINOPHILIC GRANULE
Myeloblast
Promylocyte/Progranulocyt
e
Eosinophilic Myelocyte

Eosinophilic Metamyelocyte

SIZE (IN MICRONS)

NUCLEUS, N:C RATIO

-round to oval
-central
-4:1
-round
-slightly eccentric
-3:1
-2:1 or 1:1
-slightly indented
(kidney bean shaped)
-1:1
-exceed the shape/width of nucleus
-U-shaped
-highly clumped
-1:1
-3-4 segments, connected by a large thread-like
filament
-densely packed chromatin
-1:1
NUCLEUS, N:C RATIO
-round to oval, central
-4:1
-round
-slightly eccentric
-3:1
-oval/round
-more condensed chromatin pattern
-1:1
-slightly indented
(kidney bean shaped)
-1:1

circulation for
approximatel
y 120 days

reoxygenation
-flexible

CYTOPLASM
-slightly basophilic
-no granules
-enclosed by Auer rods
-filled with granules: primary azurophilic granules

--last stage of mitosis

-secondary granules are formed
-tertiary granules are formed: gelatinase granules

-secretory vesicles/granules are formed

-specific granules (pink to rose violet)

CYTOPLASM
-slightly basophilic, no granules
-enclosed by Auer rods
-filled with granules: primary azurophilic granules

-last stage of mitosis

-bluish
large, pale, reddish orange secondary granules
-secondary granules are increased
-secretory vesicles are formed

Eosinophilic Band

Mature / Segmented / PMN

Eosinophil

-exceed the shape/width of nucleus - U-shaped

-highly clumped
-1:1
-2 lobes
-1:1

-secretory vesicles/granules are formed

-eosinophilic granules (orange)