PHYTOPLANKTON 96-h GROWTH TEST- CELLS NUMBER

Test species :
Dilution water :
Test Date :
Project Name :
Nominal
Conc.
(mg/L)

Isochrysis sp
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(
)

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C

Kontrol

0.56

1.0

1.8

3.2

5.6

I% =

C-T

x 100

C
S% =

T-C
C

x 100

Count
1
( x 104)
15
18
19
24
31
28
23
30
27
19
21
19
30
31
28
49
35
37

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
12
17
21
25
27
31
27
25
28
23
23
23
27
32
26
47
37
39

Mean
cells/mL
( x 104)
13.50
17.50
20.00
24.50
29.00
29.50
25.00
27.50
27.50
21.00
22.00
21.00
28.50
31.50
27.00
48.00
36.00
38.00

Mean
cells/mL
( x 104)
17.00

27.67

26.67

21.33

29.00

40.67

Recorder : ________
Notes
: __________________________________
__________________________________
__________________________________

- CELLS NUMBER

PHYTOPLANKTON 96-h G

100mL
1x10.000
Cadmium
24h
%
Inhibition

_______________________
_______________________
_______________________

Test species :
Dilution water :
Test Date :
Project Name :
%
Stimulation

Nominal
Conc.
(mg/L)

Isochrysis sp
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(
)

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C

Kontrol

0.56

1.0

1.8

3.2

5.6

I% =

C-T

x 100

C
S% =

T-C
C

x 100

OPLANKTON 96-h GROWTH TEST- CELLS NUMBER

ochrysis sp
NSW
5/6/2014
ompetitip 2014 Nano
Count
1
( x 104)
17
26
20
18
17
19
12
15
13
14
10
13
13
10
11
12
9
7

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
20
23
23
19
15
17
17
17
15
16
12
14
11
14
13
16
9
10

Mean
cells/mL
( x 104)
18.50
24.50
21.50
18.50
16.00
18.00
14.50
16.00
14.00
15.00
11.00
13.50
12.00
12.00
12.00
14.00
9.00
8.50

Mean
cells/mL
( x 104)

100mL
1x10.000
Cadmium
48h
%
Inhibition

21.50

17.50

18.60%

14.83

31.01%

13.17

38.76%

12.00

44.19%

10.50

51.16%

Recorder : ________
Notes
: __________________________________
__________________________________
__________________________________

%
Stimulation

PHYTOPLANKTON 96-h GROWTH TEST- CELLS NUMBER

Test species :
Dilution water :
Test Date :
Project Name :
Nominal
Conc.
(mg/L)

Isochrysis sp
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(
)

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C

Kontrol

0.56

1.0

1.8

3.2

5.6

I% =

C-T

x 100

C
S% =

T-C

x 100

Count
1
( x 104)
29
18
19
14
18
29
20
14
24
14
10
14
10
14
13
16
17
10

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
27
21
21
14
19
27
19
15
24
20
9
12
10
17
16
17
14
8

Mean
cells/mL
( x 104)
28.00
19.50
20.00
14.00
18.50
28.00
19.50
14.50
24.00
17.00
9.50
13.00
10.00
15.50
14.50
16.50
15.50
9.00

Mean
cells/mL
( x 104)
22.50

20.17

19.33

13.17

13.33

13.67

Recorder : ________
Notes
: __________________________________
__________________________________
__________________________________

CELLS NUMBER

PHYTOPLANKTON 96-h GR

100mL
1x10.000
Cadmium
72h
%
Inhibition

Test species :
Dilution water :
Test Date :
Project Name :
%
Stimulation

Nominal
Conc.
(mg/L)

Isochrysis sp
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(
)

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C

Kontrol

10.37%

0.56

14.07%

1.0

41.48%

1.8

40.74%

3.2

39.26%

5.6

______________________
______________________
______________________

I% =

C-T

x 100

C
S% =

T-C
C

x 100

TOPLANKTON 96-h GROWTH TEST- CELLS NUMBER

ochrysis sp
NSW
5/6/2014
ompetitip 2014 Nano
Count
1
( x 104)
43
32
37
21
21
31
25
21
19
24
13
22
26
28
32
18
16
20

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
39
34
35
24
20
28
22
24
20
25
12
27
27
28
35
22
15
15

Mean
cells/mL
( x 104)
41.00
33.00
36.00
22.50
20.50
29.50
23.50
22.50
19.50
24.50
12.50
24.50
26.50
28.00
33.50
20.00
15.50
17.50

Mean
cells/mL
( x 104)

100mL
1x10.000
Cadmium
96h
%
Inhibition

36.67

24.17

34.09%

21.83

40.45%

20.50

44.09%

29.33

20.00%

17.67

51.82%

Recorder : ________
Notes
: __________________________________
__________________________________
__________________________________

%
Stimulation

PHYTOPLANKTON 96-h GROWTH TEST- CELLS NUMBER

Test species :
Dilution water :
Test Date :
Project Name :
Nominal
Conc.
(/L)

Isochrysis sp
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(/L)

Kontrol

10.00

10

18.00

21

32.00

25

56.00

38

100.00

32

100 *)

62

I% =

C-T

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
x 100

C
S% =

T-C
C

x 100

Count
1
( x 104)
28
21
21
26
28
32
38
20
22
22
21
25
30
20
19
17
28
24
19
15
20

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
27
23
22
29
29
33
35
27
25
21
23
23
29
23
23
18
32
24
20
13
18

Mean
cells/mL
( x 104)
27.50
22.00
21.50
27.50
28.50
32.50
36.50
23.50
23.50
21.50
22.00
24.00
29.50
21.50
21.00
17.50
30.00
24.00
19.50
14.00
19.00

Mean
cells/mL
( x 104)
23.67

29.50

27.83

22.50

24.00

23.83

17.50

Recorder : ________
Notes
: __________________________________
__________________________________
__________________________________

- CELLS NUMBER

PHYTOPLANKTON 96-h

100mL
1x10.000
Copper
24h
%
Inhibition

_______________________
_______________________
_______________________

Test species :
Dilution water :
Test Date :
Project Name :
%
Stimulation

Nominal
Conc.
(/L)

Isochrysis sp
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(/L)

Kontrol

10.00

10

18.00

21

32.00

25

56.00

38

100.00

32

100 *)

62

I% =

C-T

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
x 100

C
S% =

T-C
C

x 100

TOPLANKTON 96-h GROWTH TEST- CELLS NUMBER

ochrysis sp
NSW
5/6/2014
ompetitip 2014 Nano
Count
1
( x 104)
20
10
21
14
20
19
12
10
11
12
19
17
20
13
10
8
8
9
8
10
8

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
17
18
19
13
21
21
10
14
8
13
15
15
18
14
13
10
12
7
10
12
10

Mean
cells/mL
( x 104)
18.50
14.00
20.00
13.50
20.50
20.00
11.00
12.00
9.50
12.50
17.00
16.00
19.00
13.50
11.50
9.00
10.00
8.00
9.00
11.00
9.00

Mean
cells/mL
( x 104)

100mL
1x10.000
Copper
48h
%
Inhibition

%
Stimulation

17.50

18.00

2.86%

10.83

38.10%

15.17

13.33%

14.67

16.19%

9.00

48.57%

9.67

44.76%

Recorder : ________
Notes
: __________________________________
__________________________________
__________________________________

PHYTOPLANKTON 96-h GROWTH TEST- CELLS NUMBER

Test species :
Dilution water :
Test Date :
Project Name :
Nominal
Conc.
(/L)

Isochrysis sp
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(/L)

Kontrol

10.00

10

18.00

21

32.00

25

56.00

38

100.00

32

100 *)

62

I% =

C-T

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
x 100

C
S% =

T-C
C

x 100

Count
1
( x 104)
23
44
56
47
45
27
31
19
29
33
42
20
23
18
23
19
20
36
21
19
14

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
27
35
54
41
48
34
30
23
21
32
40
28
28
18
29
17
23
31
22
17
13

Mean
cells/mL
( x 104)
25.00
39.50
55.00
44.00
46.50
30.50
30.50
21.00
25.00
32.50
41.00
24.00
25.50
18.00
26.00
18.00
21.50
33.50
21.50
18.00
13.50

Mean
cells/mL
( x 104)
39.83

40.33

25.50

32.50

23.17

24.33

17.67

Recorder : ________
Notes
: __________________________________
__________________________________
__________________________________

CELLS NUMBER

PHYTOPLANKTON 96-h GRO

100mL
1x10.000
Copper
72h
%
Inhibition

Test species :
Dilution water :
Test Date :
Project Name :
%
Stimulation

Nominal
Conc.
(/L)

Isochrysis sp
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(/L)

Kontrol

10.00

10

35.98%

18.00

21

18.41%

32.00

25

41.84%

56.00

38

38.91%

100.00

32

55.65%

100 *)

62

1.26%

______________________
______________________
______________________

I% =

C-T

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
x 100

C
S% =

T-C
C

x 100

PLANKTON 96-h GROWTH TEST- CELLS NUMBER

ochrysis sp
NSW
5/6/2014
ompetitip 2014 Nano
Count
1
( x 104)
40
44
41
53
45
50
41
46
34
37
32
26
31
41
28
18
12
15
14
9
11

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
42
43
47
45
49
49
34
45
35
38
30
25
28
32
30
21
11
15
13
10
10

Mean
cells/mL
( x 104)
41.00
43.50
44.00
49.00
47.00
49.50
37.50
45.50
34.50
37.50
31.00
25.50
29.50
36.50
29.00
19.50
11.50
15.00
13.50
9.50
10.50

Mean
cells/mL
( x 104)

100mL
1x10.000
Copper
96h
%
%
Inhibition Stimulation

42.83

48.50

39.17

8.56%

31.33

26.85%

31.67

26.07%

15.33

64.20%

11.17

73.93%

Recorder : ________
Notes
: IC50: 48.2 ppb
__________________________________
__________________________________

PHYTOPLANKTON 96-h GROWTH TEST- CELLS NUMBER

Test species :
Dilution water :
Test Date :
Project Name :
Nominal
Conc.
(mg/L)

Chaetoceros gracilis
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(
)

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C

Kontrol

0.56

1.0

1.8

3.2

5.6

I% =

C-T

x 100

C
S% =

T-C
C

x 100

Count
1
( x 104)
19
16
18
55
28
25
30
20
25
36
18
46
25
23
33
26
39
52

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
18
16
19
52
29
29
34
22
26
36
17
50
25
25
30
32
46
59

Mean
cells/mL
( x 104)
18.50
16.00
18.50
53.50
28.50
27.00
32.00
21.00
25.50
36.00
17.50
48.00
25.00
24.00
31.50
29.00
42.50
55.50

Mean
cells/mL
( x 104)
17.67

36.33

26.17

33.83

26.83

42.33

Recorder : ________
Notes
: __________________________________
__________________________________
__________________________________

- CELLS NUMBER

PHYTOPLANKTON 96-h

100mL
1x10.000
Cadmium
24h
%
Inhibition

_______________________
_______________________
_______________________

Test species :
Dilution water :
Test Date :
Project Name :
%
Stimulation

Nominal
Conc.
(mg/L)

Chaetoceros gracilis
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(
)

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C

Kontrol

0.56

1.0

1.8

3.2

5.6

I% =

C-T

x 100

C
S% =

T-C
C

x 100

TOPLANKTON 96-h GROWTH TEST- CELLS NUMBER

haetoceros gracilis
NSW
5/6/2014
ompetitip 2014 Nano
Count
1
( x 104)
10
7
14
18
10
13
10
14
13
18
19
19
16
10
19
12
14
11

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
11
7
15
19
10
12
10
14
15
18
17
21
18
10
22
12
16
11

Mean
cells/mL
( x 104)
10.50
7.00
14.50
18.50
10.00
12.50
10.00
14.00
14.00
18.00
18.00
20.00
17.00
10.00
20.50
12.00
15.00
11.00

Mean
cells/mL
( x 104)

100mL
1x10.000
Cadmium
48h
%
Inhibition

10.67

13.67

12.67

18.67

15.83

12.67

Recorder : ________
Notes
: __________________________________
__________________________________
__________________________________

%
Stimulation

PHYTOPLANKTON 96-h GROWTH TEST- CELLS NUMBER

Test species :
Dilution water :
Test Date :
Project Name :
Nominal
Conc.
(mg/L)

Chaetoceros gracilis
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(
)

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C

Kontrol

0.56

1.0

1.8

3.2

5.6

I% =

C-T

x 100

C
S% =

T-C
C

x 100

Count
1
( x 104)
21
25
33
29
27
22
32
23
19
25
27
16
20
19
16
20
26
21

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
27
29
30
27
27
25
37
27
21
28
26
20
23
26
23
20
23
26

Mean
cells/mL
( x 104)
24.00
27.00
31.50
28.00
27.00
23.50
34.50
25.00
20.00
26.50
26.50
18.00
21.50
22.50
19.50
20.00
24.50
23.50

Mean
cells/mL
( x 104)
27.50

26.17

26.50

23.67

21.17

22.67

Recorder : ________
Notes
: __________________________________
__________________________________
__________________________________

CELLS NUMBER

PHYTOPLANKTON 96-h GR

100mL
1x10.000
Cadmium
72h
%
Inhibition

______________________
______________________
______________________

Test species :
Dilution water :
Test Date :
Project Name :
%
Stimulation

Nominal
Conc.
(mg/L)

Chaetoceros gracilis
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(
)

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C

Kontrol

0.56

1.0

1.8

3.2

5.6

I% =

C-T

x 100

C
S% =

T-C
C

x 100

TOPLANKTON 96-h GROWTH TEST- CELLS NUMBER

haetoceros gracilis
NSW
5/6/2014
ompetitip 2014 Nano
Count
1
( x 104)
69
47
51
26
31
34
45
26
28
22
22
24
28
20
26
20
28
21

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
69
43
52
28
35
34
48
29
32
22
26
25
30
23
28
20
28
25

Mean
cells/mL
( x 104)
69.00
45.00
51.50
27.00
33.00
34.00
46.50
27.50
30.00
22.00
24.00
24.50
29.00
21.50
27.00
20.00
28.00
23.00

Mean
cells/mL
( x 104)

100mL
1x10.000
Cadmium
96h
%
Inhibition

55.17

31.33

43.20%

34.67

37.16%

23.50

57.40%

25.83

53.17%

23.67

57.10%

Recorder : ________
Notes
: __________________________________
__________________________________
__________________________________

%
Stimulation

PHYTOPLANKTON 96-h GROWTH TEST- CELLS NUMBER

Test species :
Dilution water :
Test Date :
Project Name :
Nominal
Conc.
(/L)

Chaetoceros gracilis
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(/L)

Kontrol

10.00

10

18.00

21

32.00

25

56.00

38

100.00

32

100 *)

62

I% =

C-T

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C

Count
1
( x 104)
59
42
27
29
30
41
35
25
36
29
14
11
16
18
20
12
19
21
25
31
27

T-C

Count
2
( x 104)
65
49
33
31
30
46
32
27
41
28
14
11
20
24
25
15
23
20
27
31
29

Mean
cells/mL
( x 104)
62.00
45.50
30.00
30.00
30.00
43.50
33.50
26.00
38.50
28.50
14.00
11.00
18.00
21.00
22.50
13.50
21.00
20.50
26.00
31.00
28.00

Mean
cells/mL
( x 104)
45.83

34.50

32.67

17.83

20.50

18.33

28.33

Recorder : ________
Notes
: __________________________________
__________________________________
__________________________________

x 100

C
S% =

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :

x 100

Kontrol
10
18
32
56
100
100*

24h
45.83
34.50
32.67
17.83
20.50
18.33
28.33

48h

72h

96h

50.00
45.00
40.00
35.00
30.00
25.00
20.00
15.00
10.00
5.00
0.00
Kontrol

10

18

32

56

100

- CELLS NUMBER

PHYTOPLANKTON 96-h

100mL
1x10.000
Copper
24h
%
Inhibition

Test species :
Dilution water :
Test Date :
Project Name :
%
Stimulation

Nominal
Conc.
(/L)

Chaetoceros gracilis
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(/L)

Kontrol

24.73%

10.00

10

28.73%

18.00

21

61.09%

32.00

25

55.27%

56.00

38

60.00%

100.00

32

38.18%

100 *)

62

_______________________
_______________________
_______________________

I% =

C-T

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
x 100

C
S% =

T-C
C

x 100

32

56

100

100*

YTOPLANKTON 96-h GROWTH TEST- CELLS NUMBER

haetoceros gracilis
NSW
5/6/2014
ompetitip 2014 Nano
Count
1
( x 104)
24
19
32
19
21
24
23
15
12
15
22
18
12
11
18
21
14
20
20
17
18

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
25
22
35
26
22
30
21
18
18
16
20
20
14
12
19
22
18
21
19
18
20

Mean
cells/mL
( x 104)
24.50
20.50
33.50
22.50
21.50
27.00
22.00
16.50
15.00
15.50
21.00
19.00
13.00
11.50
18.50
21.50
16.00
20.50
19.50
17.50
19.00

Mean
cells/mL
( x 104)

100mL
1x10.000
Copper
48h
%
Inhibition

26.17

23.67

9.55%

17.83

31.85%

18.50

29.30%

14.33

45.22%

19.33

26.11%

18.67

28.66%

Recorder : ________
Notes
: __________________________________
__________________________________
__________________________________

%
Stimulation

PHYTOPLANKTON 96-h GROWTH TEST- CELLS NUMBER

Test species :
Dilution water :
Test Date :
Project Name :
Nominal
Conc.
(/L)

Chaetoceros gracilis
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(/L)

Kontrol

10.00

10

18.00

21

32.00

25

56.00

38

100.00

32

100 *)

62

I% =

C-T

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
x 100

C
S% =

T-C
C

x 100

Count
1
( x 104)
31
72
35
37
28
29
31
28
22
24
27
25
28
28
23
24
21
23
27
30
26

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
36
56
38
35
30
33
30
34
28
22
29
25
31
28
24
28
23
30
27
31
30

Mean
cells/mL
( x 104)
33.50
64.00
36.50
36.00
29.00
31.00
30.50
31.00
25.00
23.00
28.00
25.00
29.50
28.00
23.50
26.00
22.00
26.50
27.00
30.50
28.00

Mean
cells/mL
( x 104)
44.67

32.00

28.83

25.33

27.00

24.83

28.50

Recorder : ________
Notes
: __________________________________
__________________________________
__________________________________

CELLS NUMBER

PHYTOPLANKTON 96-h GR

100mL
1x10.000
Copper
72h
%
Inhibition

Test species :
Dilution water :
Test Date :
Project Name :
%
Stimulation

Nominal
Conc.
(/L)

Chaetoceros gracilis
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(/L)

Kontrol

28.36%

10.00

10

35.45%

18.00

21

43.28%

32.00

25

39.55%

56.00

38

44.40%

100.00

32

36.19%

100 *)

62

______________________
______________________
______________________

I% =

C-T

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
x 100

C
S% =

T-C
C

x 100

TOPLANKTON 96-h GROWTH TEST- CELLS NUMBER

haetoceros gracilis
NSW
5/6/2014
ompetitip 2014 Nano
Count
1
( x 104)
49
45
58
42
65
36
58
33
30
49
39
47
29
28
20
29
24
19
16
13
16

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
54
46
58
45
66
31
58
35
37
56
45
47
27
27
24
34
26
19
17
18
19

Mean
cells/mL
( x 104)
51.50
45.50
58.00
43.50
65.50
33.50
58.00
34.00
33.50
52.50
42.00
47.00
28.00
27.50
22.00
31.50
25.00
19.00
16.50
15.50
17.50

Mean
cells/mL
( x 104)

100mL
1x10.000
Copper
96h
%
Inhibition

51.67

47.50

8.06%

41.83

19.03%

47.17

8.71%

25.83

50.00%

25.17

51.29%

16.50

68.06%

Recorder : ________
Notes
: IC50: 38ppb
__________________________________
__________________________________

%
Stimulation

515,000
455,000
580,000
435,000
655,000
335,000
580,000
340,000
335,000
525,000
420,000
470,000
280,000
275,000
220,000
315,000
250,000
190,000
165,000
155,000
175,000

5.711807
5.658011
5.763428
5.638489
5.816241
5.525045
5.763428
5.531479
5.525045
5.720159
5.623249
5.672098
5.447158
5.439333
5.342423
5.498311
5.39794
5.278754
5.217484
5.190332
5.243038