Biology 1 SHS

The Commission on Higher Education
in collaboration with the Philippine Normal University
INITIAL RELEASE: 13 JUNE 2016
Teaching Guide for Senior High School
GENERAL
BIOLOGY 1
SPECIALIZED SUBJECT | ACADEMIC - STEM

This Teaching Guide was collaboratively developed and reviewed by
educators from public and private schools, colleges, and universities.
We encourage teachers and other education stakeholders to email their
feedback, comments, and recommendations to the Commission on
Higher Education, K to 12 Transition Program Management Unit Senior High School Support Team at k12@ched.gov.ph. We value your
feedback and recommendations.
Development Team
Team Leader: Florencia G. Claveria, Ph.D.,
Dawn T. Crisologo
Writers: Doreen D. Domingo, Ph.D., Janet S.
Estacion, Ph.D., Mary Jane C. Flores, Ph.D.,
Aileen C. dela Cruz, Chuckie Fer Calsado,
Nolasco H. Sablan, Justin Ray M. Guce
Published by the Commission on Higher Education, 2016

Chairperson: Patricia B. Licuanan, Ph.D.
Commission on Higher Education
K to 12 Transition Program Management Unit
Office Address: 4th Floor, Commission on Higher Education,
C.P. Garcia Ave., Diliman, Quezon City
Telefax: (02) 441-0927 / E-mail Address: k12@ched.gov.ph
Consultants
THIS PROJECT WAS DEVELOPED WITH THE PHILIPPINE NORMAL UNIVERSITY.
University President: Ester B. Ogena, Ph.D.

VP for Academics: Ma. Antoinette C. Montealegre, Ph.D.
VP for University Relations & Advancement: Rosemarievic V. Diaz, Ph.D.
Ma. Cynthia Rose B. Bautista, Ph.D., CHED
Bienvenido F. Nebres, S.J., Ph.D., Ateneo de Manila University
Carmela C. Oracion, Ph.D., Ateneo de Manila University
Minella C. Alarcon, Ph.D., CHED
Gareth Price, Sheffield Hallam University
Stuart Bevins, Ph.D., Sheffield Hallam University
Technical Editor: John Donnie A. Ramos, Ph.D.

Copy Reader: Joy R. Jimena
Illustrators: Renan U. Ortiz, Daniela Louise B. Go
Cover Artists: Paolo Kurtis N. Tan, Renan U. Ortiz
Senior High School Support Team
CHED K to 12 Transition Program Management Unit

Program Director: Karol Mark R. Yee
Lead for Senior High School Support:
Gerson M. Abesamis
Course Development Officers:
John Carlo P. Fernando, Danie Son D. Gonzalvo
Lead for Policy Advocacy and Communications:
Averill M. Pizarro
Teacher Training Officers:
Ma. Theresa C. Carlos, Mylene E. Dones
Monitoring and Evaluation Officer:
Robert Adrian N. Daulat
Administrative Officers:
Ma. Leana Paula B. Bato, Kevin Ross D. Nera,
Allison A. Danao, Ayhen Loisse B. Dalena
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Table of Contents
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
DepEd General Biology 1 Curriculum Guide . . . . . . . . . . . . .
Chapter 3: Energy Transformation
Chapter 1: Cell
Lesson 11: Photosynthesis and Cellular Respiration . . . . . . . . . . .
86
Lesson 1: The Cell: Endomembrane System, Mitochondria,
99
Chloroplasts, Cytoskeleton, and Extracellular Components . . .
Lesson 12: Forms of Energy, Laws of Energy Transformation

and Role of ATP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Lesson 2: Mitochondria and Chloroplasts . . . . . . . . . . . . . . . . .
15
Lesson 13: Energy Transformation Part 1 . . . . . . . . . . . . . . . . . . . . 111

Lesson 3: Structure and Functions of Animal Tissues and Cell

Modification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
28
Lesson 4: Cell Cycle and Cell Division . . . . . . . . . . . . . . . . . . . . 36
Lesson 16: Cellular Respiration Part 1 . . . . . . . . . . . . . . . . . . . . . .
133
Lesson 5: Transport Mechanisms Part 1 . . . . . . . . . . . . . . . . . . . 46
150
Lesson 6: Transport Mechanisms Part 2 . . . . . . . . . . . . . . . . . . . 50
165
Chapter 2: Biological Molecules
Lesson 19: ATP in Cellular Metabolism and Photosynthesis . . . . .
176
Lesson 7: Carbohydrates and Lipids . . . . . . . . . . . . . . . . . . . . .
57
Lesson 8: Amino Acids and Proteins Part 1 . . . . . . . . . . . . . . . .
70
Lesson 9: Amino Acids and Proteins Part 2 . . . . . . . . . . . . . . . .
73
Lesson 10: Biological Molecules: Enzymes . . . . . . . . . . . . . . . .
78
Biographical Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 184
Introduction
As the Commission supports DepEds implementation of Senior High School (SHS), it upholds the vision
and mission of the K to 12 program, stated in Section 2 of Republic Act 10533, or the Enhanced Basic
Education Act of 2013, that every graduate of basic education be an empowered individual, through a
program rooted on...the competence to engage in work and be productive, the ability to coexist in
fruitful harmony with local and global communities, the capability to engage in creative and critical
thinking, and the capacity and willingness to transform others and oneself.
To accomplish this, the Commission partnered with the Philippine Normal University (PNU), the National
Center for Teacher Education, to develop Teaching Guides for Courses of SHS. Together with PNU, this
Teaching Guide was studied and reviewed by education and pedagogy experts, and was enhanced with
appropriate methodologies and strategies.
Furthermore, the Commission believes that teachers are the most important partners in attaining this
goal. Incorporated in this Teaching Guide is a framework that will guide them in creating lessons and
assessment tools, support them in facilitating activities and questions, and assist them towards deeper
content areas and competencies. Thus, the introduction of the SHS for SHS Framework.
SHS for SHS

Framework
The SHS for SHS Framework, which stands for Saysay-Husay-Sarili for Senior High School, is at the
core of this book. The lessons, which combine high-quality content with flexible elements to
accommodate diversity of teachers and environments, promote these three fundamental concepts:
SAYSAY: MEANING
HUSAY: MASTERY
SARILI: OWNERSHIP
Why is this important?
How will I deeply understand this?
What can I do with this?
Through this Teaching Guide,

teachers will be able to facilitate
an understanding of the value
of the lessons, for each learner
to fully engage in the content
on both the cognitive and
affective levels.
Given that developing mastery

goes beyond memorization,
teachers should also aim for
deep understanding of the
subject matter where they lead
learners to analyze and
synthesize knowledge.
When teachers empower

learners to take ownership of
their learning, they develop
independence and selfdirection, learning about both
the subject matter and
themselves.
About this
Teaching Guide
Biology I is a Science, Technology, Engineering and Mathematics (STEM) Specialized Subject

taken in the first half of Grades 11/12. Learners go on a journey geared toward the deeper
understanding and appreciation of life processes at the cellular and molecular levels
previously introduced in Grades 7-10. They will also apply basic chemistry and physics
principles as they examine the transformation of energy in organisms.
Implementing this course at the senior high school level is subject to numerous challenges
with mastery of content among educators tapped to facilitate learning and a lack of
resources to deliver the necessary content and develop skills and attitudes in the learners,
being foremost among these.
In support of the SHS for SHS framework developed by CHED, these teaching guides were
crafted and refined by biologists and biology educators in partnership with educators from
focus groups all over the Philippines to provide opportunities to develop the following:
Saysay through meaningful, updated, and context-specific content that highlights important
points and common misconceptions so that learners can connect to their real-world
experiences and future careers;
Husay through diverse learning experiences that can be implemented in a resource-poor
classroom or makeshift laboratory that tap cognitive, affective, and psychomotor domains
are accompanied by field-tested teaching tips that aid in facilitating discovery and
development of higher-order thinking skills; and
Sarili through flexible and relevant content and performance standards allow learners the
freedom to innovate, make their own decisions, and initiate activities to fully develop their
academic and personal potential.
These ready-to-use guides are helpful to educators new to either the content or biologists
new to the experience of teaching Senior High School due to their enriched content
presented as lesson plans or guides. Veteran educators may also add ideas from these
guides to their repertoire. The Biology Team hopes that this resource may aid in easing the
transition of the different stakeholders into the new curriculum as we move towards the
constant improvement of Philippine education.
Parts of the
Teaching Guide
This Teaching Guide is mapped and aligned to the DepEd SHS Curriculum, designed to be highly
usable for teachers. It contains classroom activities and pedagogical notes, and is integrated with
innovative pedagogies. All of these elements are presented in the following parts:
1.
2.
3.
4.
5.
6.
Introduction
Highlight key concepts and identify the essential questions
Show the big picture
Connect and/or review prerequisite knowledge
Clearly communicate learning competencies and objectives
Motivate through applications and connections to real-life
Motivation
Give local examples and applications
Engage in a game or movement activity
Provide a hands-on/laboratory activity
Connect to a real-life problem
Instruction/Delivery
Give a demonstration/lecture/simulation/hands-on activity
Show step-by-step solutions to sample problems
Give applications of the theory
Connect to a real-life problem if applicable
Practice
Discuss worked-out examples
Provide easy-medium-hard questions
Give time for hands-on unguided classroom work and discovery
Use formative assessment to give feedback
Enrichment
Provide additional examples and applications
Introduce extensions or generalisations of concepts
Engage in reflection questions
Encourage analysis through higher order thinking prompts
Evaluation
Supply a diverse question bank for written work and exercises
Provide alternative formats for student work: written homework, journal, portfolio, group/individual
projects, student-directed research project
On DepEd Functional Skills and CHED College Readiness Standards

As Higher Education Institutions (HEIs) welcome the graduates of
the Senior High School program, it is of paramount importance to
align Functional Skills set by DepEd with the College Readiness
Standards stated by CHED.
The DepEd articulated a set of 21st century skills that should be
embedded in the SHS curriculum across various subjects and tracks.
These skills are desired outcomes that K to 12 graduates should
possess in order to proceed to either higher education,
employment, entrepreneurship, or middle-level skills development.
On the other hand, the Commission declared the College

Readiness Standards that consist of the combination of knowledge,
skills, and reflective thinking necessary to participate and succeed without remediation - in entry-level undergraduate courses in
college.
The alignment of both standards, shown below, is also presented in
this Teaching Guide - prepares Senior High School graduates to the
revised college curriculum which will initially be implemented by AY
2018-2019.
College Readiness Standards Foundational Skills
DepEd Functional Skills
Produce all forms of texts (written, oral, visual, digital) based on:
1.
2.
3.
4.
5.
Solid grounding on Philippine experience and culture;

An understanding of the self, community, and nation;
Visual and information literacies, media literacy, critical thinking
Application of critical and creative thinking and doing processes;
and problem solving skills, creativity, initiative and self-direction
Competency in formulating ideas/arguments logically, scientifically, and creatively; and
Clear appreciation of ones responsibility as a citizen of a multicultural Philippines and a
diverse world;
Systematically apply knowledge, understanding, theory, and skills for the development of
the self, local, and global communities using prior learning, inquiry, and experimentation
Global awareness, scientific and economic literacy, curiosity,

critical thinking and problem solving skills, risk taking, flexibility
and adaptability, initiative and self-direction
Work comfortably with relevant technologies and develop adaptations and innovations for
significant use in local and global communities
Global awareness, media literacy, technological literacy,

creativity, flexibility and adaptability, productivity and
accountability
Communicate with local and global communities with proficiency, orally, in writing, and
through new technologies of communication
Global awareness, multicultural literacy, collaboration and

interpersonal skills, social and cross-cultural skills, leadership
and responsibility
Interact meaningfully in a social setting and contribute to the fulfilment of individual and
shared goals, respecting the fundamental humanity of all persons and the diversity of
groups and communities
Media literacy, multicultural literacy, global awareness,

collaboration and interpersonal skills, social and cross-cultural
skills, leadership and responsibility, ethical, moral, and spiritual
values
General Biology 1
60 MINS
The Cell: Endomembrane System, Mitochondria,
Chloroplasts, Cytoskeleton, and Extracellular Components

Content Standards
The learners demonstrate an understanding of (1) Composition of the
endomembrane system; (2) Structure and function of organelles involved in
energy transformation; (3) Structure and functions of the cytoskeleton; and, (4)
Composition and functions of the extracellular components or matrix.
Performance Standards
The learners shall be able to construct three-dimensional models of whole cells
using indigenous or recyclable materials. The models shall show the following
cell parts: (1) Endomembrane System, (2) Mitochondria, and (3) Chloroplast
Learning Competencies
The learners: (1) explain the postulates of the cell theory (STEM_BIO11/12-1ac-1); (2) describe the structure and function of major and subcellular organelles
(STEM_BIO11/12-Ia-c-2); (3) describe the structural components of the cell
membrane (STEM_BIO11/12-Ig-h-11); and (4) relate the structure and
composition of the cell membrane to its function (STEM_BIO11/12-Ig-h-12)
Specific Learning Outcomes
At the end of the unit lesson, the learners shall be able to:
illustrate the structure of the endomembrane system, label its parts, and
understand how the system works
illustrate the structure of the mitochondria, label its parts, and understand
the importance of the enfolding of the inner mitochondrial membrane
illustrate the structure of the chloroplast, label its parts, and relate these
parts to photosynthesis
understand the connection of the endomembrane system to other cell
parts such as the lysosomes, peroxisomes, endosomes, and cell membrane
understand how the extracellular components or matrix determine the
appearance and function of the tissues
LESSON OUTLINE
Introduction Review on the differences between
prokaryotic and eukaryotic cells; submission

and discussion of responses to the pre-topic
homework assigned before the lecture.
Motivation
Brief class activity on prokaryotic and

eukaryotic cells.
Instruction/ Lecture. Board work on cell parts, structure,

Practice
and function. Examination of cheek cells and
40
Hydrilla cells under a microscope. Class

activity on identifying the parts and functions
of the endomembrane system.
Enrichment Class discussion on cell size and relationship

of surface area and volume
Evaluation
Assessment of learners knowledge;

assignment of homework for next lecture
Materials
microscope (slide, cover slip), hand-held

lens, work books, methylene blue, plastic
spoon/popsicle stick, Hydrilla plansts,
colored chalk/white board marker
5
5
Resources (continued at the end of Teaching Guide)

(1) (n.d.). Retrieved from <http://www.phschool.com/science/
biology_place/biocoach/cells/common.html>
(2) (n.d.). Retrieved from <http://biology.tutorvista.com/animal-and-plant(3) (n.d.). Retrieved from <http://sciencenetlinks.com/lessons/cells-2-thecell-as-a-system/>
INTRODUCTION (5 MINS)
Teacher Tip
1. Ask the learners to make a recap of the differences between prokaryotic and eukaryotic cells.
2. Discuss the learners responses to the pre-topic assignment on the functions of the following cell
parts:
Nucleus
The review on the differences between

prokaryotic and eukaryotic cells is needed
to connect prerequisite knowledge to the
present lesson. Remind the learners that the
cell parts are found in eukaryotic cells.
Remind the learners of the pre-topic
assignment that shall be submitted before
the lecture. This is to ensure the learners
read on the topic before the lecture.
Smooth Endoplasmic Reticulum

Rough Endoplasmic Reticulum
Golgi Apparatus
Ribosomes
Briefly discuss the structure of the cell

membrane in order to provide basic
knowledge on said structure to the learners.
Do not fully elaborate on this topic since the
structure and function of the cell membrane
shall further be discussed in the succeeding
parts of the lesson.
Lysosomes
Mitochondria
Chloroplast
3. Present an overview of the cell membrane, its structure, and functions.
4. Define what an organelle is and differentiate membrane-bound organelles from non-membranebound organelles.
5. Explain that in eukaryotic cells, the machinery of the cell is compartmentalized into organelles. The
compartmentalization of the cell into membrane-bound organelles:
allows conflicting functions (i.e., synthesis vs. breakdown) and several cellular activities to occur
simultaneously without interference from each other
separates the DNA material of the nucleus, mitochondria, and chloroplast
increases the surface area-volume ratio of the cell
6. Encourage the learners to look at the cell as both a system and subsystem. They should develop an
understanding of how the parts of a cell interact with one another and how these parts help to do the
work of the cell (Source: (n.d.). Retrieved from <http://sciencenetlinks.com/lessons/cells-2-the-cell-asa-system/>)
10
The cells parts should be discussed as a

system, emphasizing on the
interconnectedness of each part to the
others.
To clarify common misconceptions,
emphasize the following to the learners:
Not all organelles are surrounded by a
membrane.
The plasma or cell membrane is different
from the cell wall.
Not all cell parts are present in all kinds of
cells.
MOTIVATION (5 MINS)
Briefly review the differences between prokaryotic and eukaryotic cells by asking questions to the
learners.
Sample question: What cell parts can be found in both prokaryotic and eukaryotic cells? Discuss
the function/s of each part.
Sample Responses:
DNA
Cell membrane
Protoplasm (nucleoloid region and cytosol)
Ribosomes
Compare the cell to a big city. Ask the learners what the requirements of the city would be in order for
it to function. Relate these requirements to the parts of the cell. Relate the learners responses to the
functions of the different parts of a cell.
Sample responses:
The city will need power. What generates power for the city? Relate this to the function of
the mitochondria and the chloroplast.
The city generates waste. How does it minimize its waste? How does the city handle its
garbage? Relate this to the function of the lysosome.
The city requires raw materials to process into food, clothing, and housing materials. Where
are these raw materials processed? Relate this to the functions of the Golgi Apparatus.
Compare animal cells from plant cells. For the animal cells, scrape cheek cells using a toothpick. Ask
the learners to place the scrapings on a microscope slide and add a drop of water to the scrapings.
Tease the scrapings into a thin layer and cover with a slip. Examine under HPO. Instruct the learners to
draw the cells on their workbooks and to label the cell parts that they were able to observe under the
microscope.
For the plant cells, instruct the learners to obtain a Hydrilla leaf and place it on a microscope slide.
Examine under LPO. Ask the learners to draw the cells on their workbooks and to label the cell parts
that they were able to observe under the microscope.
Teacher tip
If the number of available microscopes is
limited, ask the learners to group
themselves according to the number of
microscopes available or set-up a
demonstration scope for the whole class
and facilitate the examination of cells so
that all the learners will get a chance to
observe the cells under the microscope.
Orient the learners on the proper use and
care of the microscopes, particularly on
focusing first on LPO before shifting to
HPO.
Cheek cells are very transparent. Adjust the
iris diaphragm or add a small amount of dye
(i.e., methylene blue) to the scrapings.
The learners will only see the cell membrane
and the nucleus. Remind the learners to
draw what they observe. Students may
observe cytoplasmic streaming in the plant
cell.
INSTRUCTION/PRACTICE (30 MINS)

1. Draw the cell membrane on one end of the board.
2. Draw the double membrane of the nucleus (nuclear membrane) on the other end of the board.
3. From the nuclear membrane, draw the reticulated structure of the endoplasmic reticulum. Ask the
learners what the two types of endoplasmic reticulum are and their corresponding functions.
4. Draw the ribosomes as separate units.
5. Draw a DNA and an mRNA. Explain that the mRNA is a copy of the DNA that will be sent to the
cytoplasm for protein synthesis.
6. Explain to the learners that the mRNA leaves the nucleus and goes to where the ribosomes are
located (i.e., mRNA + functional ribosome)
7. Explain the possible pathways for protein synthesis (e.g., within the cytosol or the endoplasmic
reticulum)
8. Draw the mRNA + functional ribosome on the endoplasmic reticulum. With a lot of these, the
endoplasmic reticulum becomes a rough endoplasmic reticulum.
9. Draw the formed polypeptide inside the rough endoplasmic reticulum. Discuss the formation of a
cisternae and pinching off as a vesicle.
10. Draw the Golgi Apparatus and then a vesicle from the rough endoplasmic reticulum that travels to
the Golgi Apparatus and attaches to the part which is nearest the rough endoplasmic reticulum.
11. Ask the learners what the function of the Golgi Apparatus is. Synthesize their answers and compare
the Golgi Apparatus to a factory with an assembly manufacturing line.
12. Draw the polypeptide travelling along the Golgi Apparatus stack; pinching off as a vesicle to travel
to the next stack. Repeat the process while increasing the complexity of the polypeptide drawing.
13. On the last stack, explain the pathways that the vesicle may follow: become a lysosome through
fusion with an endosome (i.e., formed by endocytosis), or travel to the cell membrane, fuse with it,
and empty its contents.
14. Present the composition of the endomembrane system and discuss how these parts are connected
to each other by structure and by function.
15. Draw the mitochondria and label its parts. Explain the importance of the enfolding (cristae) in
increasing the surface area of the inner mitochondrial membrane. Further explain to the class that
Teacher tip
Use chalk or white board markers with
different colors. Explain the structure and
function of each cell part as you draw them.
Explain to the learners that a more detailed
discussion of the structure and functions of
the cell membrane, mitochondria, and
chloroplast will be given in succeeding
lessons.
enfolding is a common structural strategy to increase surface area. As an example, you may draw a
cross-sectional structure of the small intestine.
16. Draw the chloroplast and label its parts. Explain the function that each part performs in the process
of photosynthesis.
17. Discuss the similarities of the mitochondria and chloroplast (e.g., both are involved in energy
transformation, both have DNA, high surface area, and double membranes).income accounts and
lastly, expenses accounts.
Group the learners into pairs. Ask one to draw the endomembrane system as he/she explains it to
his/her partner. Reshuffle the groupings and repeat until all learners have performed the exercise.
ENRICHMENT (30 MINS)
Facilitate a class discussion on why cells are generally small in size. Explain the relationship between
surface area and volume.
EVALUATION (60 MINS
Ask questions to the learners. Sample questions can be found in the following electronic resources:
Teacher tip
(n.d.). Retrieved from< http://www.proprofs.com/quiz-school/story.php?title=cell-structure-test >

(n.d.). Retrieved from< http://study.com/academy/exam/topic/cell-biology.html>
Assign a research assignment on this question: How do environmental toxins like lead and mercury
affect the functions of the cell? The assignment shall be submitted one week after this lesson.
Assignments should be handwritten.
RESOURCES (CONTINUED):
(4) (n.d.). Retrieved from <http://www.schools.manatee.k12.fl.us/072JOCONNOR/celllessonplans/

lesson_plan__cell_structure_and_function.html>
(5) (n.d.). Retrieved from <http://www.phschool.com/science/biology_place/biocoach/cells/endo.html>
(6) (n.d.). Retrieved from <http://study.com/academy/lesson/the-endomembrane-system-functionscomponents.html>
(7) (n.d.). Retrieved from <http://www.ncbi.nlm.nih.gov/books/NBK26907/>
(8) (n.d.). Retrieved from <http://staff.um.edu.mt/acus1/01Compart.pdf>
This strategy is aimed at ensuring that the

learners have read the topic rather than just
copying and printing from a source.
ASSESSMENT
Learning Competency
Assessment Tool
Exemplary
Learner was able to

Learner
participation (during answer all the question/s
without referring to his/
1. describe the structure and lecture)
her notes
function of major and
The learners shall be able
to:
subcellular organelles
(STEM_BIO11/12-Ia-c-2)

to:
2. describe the structural
components of the cell
membrane
(STEM_BIO11/12-Ig-h-11)

to:
3. relate the structure and
composition of the cell
membrane to its function
(STEM_BIO11/12-Ig-h12)
Assignment
Learner submitted an
assignment beyond the
requirements
Learner was able to

Learner
participation (during concisely answer all the
questions
practice)
Satisfactory
Developing
Learner was able to answer

the main question without
referring to his/her notes
but was not able to answer
follow-up question/s
Learner was able to

answer the questions
but he/she referred
to his/her notes
(1) Learner was not

able to answer the
question/s
Learner submitted a
comprehensive and wellwritten assignment
Learner submitted a
well written report
but some responses
lack details
(1) Learner did not

submit an assignment
Learner was able to answer

the main question without
referring to his/her notes
but was not able to answer
follow-up question/s
Learner was able to

answer the questions
but he/she referred
to his/her notes
Learner submitted drawings

that fulfilled the
requirements (complete
and detailed)
Learner submitted
drawings that were
incomplete
(1) Learner was not

able to submit
drawings
(2) Learners drawings
were haphazardly
done
Learner obtained less
that 50% correct
answers in the
examination
Laboratory
(Examination of
Animal and Plant
Cells)
Learner submitted
drawings that were
beyond the requirements
Examination
Learner obtained 90% to

100% correct answers in
the examination

89.99% correct answers in
the examination
Learner obtained
50% to 69.99%
correct answers in the
examination
Research
Assignment
Learner submitted a
research assignment
beyond the requirements
Learner submitted a
comprehensive and wellwritten research assignment
Learner submitted a
well written report
but some responses
lack details
14
Beginnning
(2) Learner read notes

of his/her classmate
(2) Learner submitted

a partially-finished
assignment
(1) Learner was not
able to answer the
question/s
(2) Learner read notes
of his/her classmate
(1) Learner did not

submit an assignment
(2) Learner submitted
a partially-finished
assignment
General Biology 1
60 MINS
Mitochondria and Chloroplasts

Content Standards
LESSON OUTLINE
The learners demonstrate an understanding of the structure and function of the
mitochondria and chloroplasts, the organelles involved in energy
Introduction Review of relevant terminologies and
definitions
transformation.
using indigenous or recyclable materials. These models should show the
mitochondria and chloroplasts.
Motivation
Instruction/ Discussion and lecture proper

Delivery
30
The learners describe the structure and function of major and subcellular
organelles (STEM_BIO11/12-Ia-c-2) and distinguish prokaryotic and eukaryotic
cells according to their distinguishing features (STEM_BIO11/12 -Ia-c-3)
Practice
10
Understanding of key concepts using real-life

situations
Drawing (with label) activity
Enrichment Computation of surface area vs volume
Evaluation
Answering practice questions and homework

At the end of the lesson, the learners shall be able to:
(1) http://scienceaid.co.uk/biology/biochemistry/atp.html
(2) http://www.britannica.com/list/6-cell-organelles)
(3) http://www.nature.com/scitable/topicpage/mitochondria-14053590)
(4) http://www.britannica.com/list/6-cell-organelles
(6) http://biology.tutorvista.com/animal-and-plant-cells/chloroplasts.html
(7) ttp://www.nature.com/scitable/topicpage/mitochondria-14053590
Facilitate a review of the following concepts:
Differences between prokaryotic and eukaryotic cells

Definition of an organelle
Differences between membrane-bound organelles and non-membrane-bound organelles
Functions of the different parts of a cell
The endomembrane system
MEMBRANE-BOUND ORGANELLES
NON-MEMBRANE-BOUND ORGANELLES
Nucleus
Ribosomes
Smooth ER
Centrioles
Rough ER
Cytoskeleton
Golgi Apparatus
Vacuoles and Vesicles
Mitochondria
Chloroplast and other plastids
Lysosomes
Peroxisomes
Explain that in eukaryotic cells, the machinery of the cell is compartmentalized into organelles. The compartmentalization of the cell into
membrane-bound organelles:
allows conflicting functions (i.e., synthesis vs. breakdown) and several cellular activities to occur simultaneously without interference from
each other
16
Encourage the learners to look at the cell as both a system and subsystem. They should develop an
Emphasize to the learners that energy transformation is one of the characteristics of life. This refers to
the ability to obtain and use energy. This characterizes the main function of the mitochondria and the
chloroplasts.
MOTIVATION (5 MINS)
Ask the learners how they understand the concept of compartmentalization. Relate the concept to how
the cell is compartmentalized into organelles.
Compare compartmentalization to the division of a house into a receiving room or sala, kitchen, dining
room, comfort rooms, bedrooms, etc.
Teacher tip
Ask the learners why they think a house is divided into several rooms.
A possible response is that partitioning of the house into different parts facilitates the simultaneous
occurrence of several activities without interfering with one another. Also, materials needed for each
activity can be stored at their specific areas. For example, pots and pans are being stored in the kitchen
and not in the bedroom. Beds and pillows are found in the bedroom and not in the toilet/bath.
Explain to the learners that the mitochondria and chloroplasts have a small amount of DNA. Although
most of the proteins of these organelles are imported from the cytosol and are thus programmed by
the nuclear DNA, their DNA programs the synthesis of the proteins made on the organelles ribosomes
(Source: Campbell et al). Compartmentalization separates the DNA material of the nucleus,
mitochondria, and chloroplast.
Ask the learners if they have experienced going to a city/municipal hall and if they have observed that
the Mayor, Vice-Mayor, and the City/Municipal Administrator have separate offices. You can use other
examples such as the University President, VP for Academic Affairs, VP for Finance; Philippine
President, Vice President, Senators, etc.
Compare the nuclear DNA to the Mayor and the mitochondrial DNA and chloroplast DNA to the Vice
Explain to the learner that this is how the

cell is able to allow conflicting functions
(e.g., synthesis vs breakdown) and several
cellular activities to occur simultaneously
without interference from each other.
Mayor. The Mayor runs the city/municipality but the Vice Mayor also performs functions that are
specific to their positions. They need different offices (or compartments) to avoid conflict in their
functions.
Teacher tip
Select a fruit that can be easily peeled like
calamansi or dalandan
Introduce the concept of surface area-volume ratio/relationship to the learners. Show a fruit to the
learners and explain that the outer surface of the fruit is the surface area. Peel the fruit and show them
whats inside, explaining that the inside of the fruit is the volume.
Explain to the learners that surface area (SA) and volume (V) do not increase in the same manner. As an
object increases in size, its volume increases as the cube of its linear dimensions while surface area
increases as the square of its linear dimensions.
Example: If the initial starting point is the same: SA = 2; Volume = 2 (Ratio = 1:1)
A one-step increase will result to: SA = 22 = 4 while V = 23 = 8 (Ratio = 1:2)
Teacher tip
Ask questions to the learners while giving
the lecture.
INSTRUCTION/DELIVERY (30 MINS)

Explain and discuss the nature and functions of the Adenosine Triphosphate (ATP) to the learners.
Adenosine Triphosphate (ATP)It is the majorenergy currencyof the cell that provides the energy for
most of the energy-consuming activities of the cell. The ATP regulates many biochemical pathways.
Mechanism: When the third phosphate group of ATP is removed by hydrolysis, a substantial amount of
free energyis released.
ATP + H2O ADP + Pi where ADP is adenosine diphosphate and Piis inorganic phosphate
Group the learners into pairs. Ask one to draw the endomembrane system as he/she explains it to his/
her partner. Reshuffle the groupings and repeat until all learners have performed the exercise.
18
If an LCD projector is not available, draw

the structure of the mitochondria and
chloroplast on the board.
Illustration 1: Energy release in Hydrolysis (Source: (n.d.). Retrieved from http://scienceaid.co.uk/biology/biochemistry/atp.html)
Illustration 2: Chemical Energy and ATP (Source: (n.d.). Retrieved from http://winklebiology.weebly.com/chemical-energyatp.html)
Synthesis of ATP
ADP + Pi ATP + H2O
requires energy: 7.3 kcal/mole
occurs in the cytosol by glycolysis
occurs in mitochondria by cellular respiration

occurs in chloroplasts by photosynthesis
Consumption of ATP
ATP powers most energy-consuming activities of cells, such as:
anabolic (synthesis) reactions, such as:

joining transfer RNAs to amino acids for assembly into proteins
synthesis of nucleoside triphosphates for assembly into DNA and RNA
synthesis of polysaccharides
synthesis of fats
active transport of molecules and ions
conduction of nerve impulses
maintenance of cell volume by osmosis
addition of phosphate groups (phosphorylation) to different proteins (e.g., to alter their activity incell
signaling)
muscle contraction
beating of cilia and flagella (including sperm)
bioluminescence
Extracellular ATP
In mammals, ATP also functions outside of cells. ATP is released in the following examples:
from damaged cells to elicit inflammation andpain

from thecarotid body to signal a shortage of oxygen in the blood
fromtaste receptor cells to trigger action potentials in the sensory nerves leading back to the brain
from the stretched wall of the urinary bladder to signal when the bladder needs emptying
In eukaryotic cells, the mitochondria and chloroplasts are the organelles that convert energy to other
forms which cells can use for their functions.
Discuss the function and structure of the mitochondria.
20
Mitochondria (singular, mitochondrion)Mitochondria are the sites of cellular respiration, the

metabolic process that uses oxygen to drive the generation of ATP by extracting energy from sugars,
fats, and other fuels.
The mitochondria are oval-shaped organelles found in most eukaryotic cells. They are considered to be
the powerhouses of the cell. As the site of cellular respiration, mitochondria serve to transform
molecules such as glucose into an energy molecule known as adenosine triphosphate (ATP). ATP fuels
cellular processes by breaking its high-energy chemical bonds. Mitochondria are most plentiful in cells
that require significant amounts of energy to function, such as liver and muscle cells.
Figure 1: Structure of the Mitochonsdria (Source: (n.d.). Retrieved from http://www.britannica.com/list/
6-cell-organelles)
The mitochondria has two membranes that are similar in composition to the cell membrane:
Outer membraneis a selectively permeable membrane that surrounds the mitochondria. It is the
site of attachment for the respiratory assembly of the electron transport chain and ATP Synthase. It
has integral proteins and pores for transporting molecules just like the cell membrane
Inner membranefolds inward (called cristae) to increase surfaces for cellular metabolism. It
contains ribosomes and the DNA of the mitochondria. The inner membrane creates two enclosed
spaces within the mitochondria:
intermembrane space between the outer membrane and the inner membrane; and
matrix that is enclosed within the inner membrane.
Ask questions to the learners on the structure of the mitochondria. A sample question could be: What
is the importance of the enfolding of the mitochondria? The response would be to increase the surface
area that can be packed into such a small space.
Discuss the purpose of the mitochondrial membranes.
22
As mentioned, the mitochondria has two membranes: the outer and inner mitochondrial membranes.
Outer Membrane
fully surrounds the inner membrane, with a small intermembrane spacein between
has many protein-based pores that are big enough to allow the passage of ions and
molecules as large as a small protein
Inner membrane
has restricted permeability like the plasma membrane
is loaded with proteins involved in electron transport and ATP synthesis
surrounds the mitochondrial matrix, where the citric acid cycle produces the electrons that
travel from one protein complex to the next in the inner membrane. At the end of this
electron transport chain, the final electron acceptor is oxygen, and this ultimately forms
water (H20). At the same time, the electron transport chain produces ATP in a process called
oxidative phosphorylation
During electron transport, the participating protein complexes push protons from the matrix out to the
intermembrane space. This creates a concentration gradient of protons that another protein complex,
calledATP synthase, uses to power synthesis of the energy carrier molecule ATP.
Figure 4: The Electrochemical Proton Gradient and the ATP Synthase (Source: (n.d.). Retrieved from
http://www.nature.com/scitable/topicpage/mitochondria-14053590)
Explain and discuss the structure and functions of the Chloroplasts.
ChloroplastsChloroplasts, which are found in plants and algae, are the sites of photosynthesis. This
process converts solar energy to chemical energy by absorbing sunlight and using it to drive the
synthesis of organic compounds such as sugars from carbon dioxide and water.
The word chloroplast is derived from the Greek word chloros which means green and plastes which
means the one who forms. The chloroplasts are cellular organelles of green plants and some
eukaryotic organisms. These organelles conduct photosynthesis. They absorb sunlight andconvert it
into sugar molecules. They also produce free energy stored in the form of ATP and NADPHthrough
photosynthesis.
Chloroplasts are double membrane-bound organelles and are the sites of photosynthesis. The
22
Teacher tip
Lecture on mitochondrial membranes can

be accessed at (n.d.). Retrieved from
<http://www.nature.com/scitable/
topicpage/mitochondria-14053590>.
chloroplast has a system of three membranes: the outer membrane, the inner membrane, and the
thylakoid system.The outer and the inner membranes of the chloroplast enclose a semi-gel-like fluid
known as the stroma. The stroma makes up much of the volume of the chloroplast. The thylakoid
system floats in the stroma.
Structure of the Chloroplast
Outer membraneThis is a semi-porous membrane and is permeable to small molecules and ions
which diffuse easily. The outer membrane is not permeable to larger proteins.
Intermembrane SpaceThis is usually a thin intermembrane space about 10-20 nanometers and is
present between the outer and the inner membrane of the chloroplast.
Inner membraneThe inner membrane of the chloroplast forms a border to the stroma. It
regulates passage of materials in and out of the chloroplast. In addition to the regulation activity,
fatty acids, lipids and carotenoids are synthesized in the inner chloroplast membrane.
StromaThis is an alkaline, aqueous fluid that is protein-rich and is present within the inner
membrane of the chloroplast. It is the space outside the thylakoid space. The chloroplast DNA,
chloroplast ribosomes, thylakoid system, starch granules, and other proteins are found floating
around the stroma.
Thylakoid System
The thylakoid system is suspended in the stroma. It is a collection of membranous sacks called
thylakoids. Thylakoids are small sacks that are interconnected. The membranes of these thylakoids are
the sites for the light reactions of the photosynthesis to take place. The chlorophyll is found in the
thylakoids. The thylakoids are arranged in stacks known as grana. Each granum contains around 10-20
thylakoids.
The wordthylakoid is derived from the Greek word thylakos which means 'sack'.
Important protein complexes which carry out the light reaction of photosynthesis are embedded in the
membranes of the thylakoids.
The Photosystem I and the Photosystem II are
Teacher tip
the structure of the chloroplast on the
board.
Lecture on structure and functions of the

chloroplast can be accessed at (n.d.).
Retrieved from <http://
biology.tutorvista.com/animal-and-plantcells/chloroplasts.html>.
complexes that harvest light with chlorophyll and carotenoids. They

absorb the light energy and use it to energize the electrons.
The molecules present in the thylakoid membrane use the electrons
that are energized to pump hydrogen ions into the thylakoid space.
This decreases the pH and causes it to becomeacidic in nature. A
large protein complex known as the ATP synthase controls the
concentration gradient of the hydrogen ions in the thylakoid space
to generate ATP energy. The hydrogen ions flow back into the
stroma.
PRACTICE (10 MINS)
Thylakoids areof two types: granal thylakoids and stromal

thylakoids. Granal thylakoids are arranged in the grana. These
circular discs that are about 300-600 nanometers in diameter. The
stromal thylakoids are in contact with the stroma and are in the form
of helicoid sheets.
Group the learners into pairs. Ask one to draw the mitochondria and
label its parts while the other does the same for chloroplast. Once
done, the partners exchange tasks (i.e., the learner that drew the
mitochondria now does the same for the chloroplast).
The granal thylakoids contain only Photosystem II protein complex.

This allows them to stack tightly and form many granal layers with
granal membrane. This structure increases stability and surface area
for the capture of light.
Reproduce these diagrams without the labels and use these for the
class activity.
To demonstrate how folding increases surface area, ask the learners
to trace the edges of the outer membrane with a thread and
measure the length of the thread afterwards. Repeat the same for
the inner membrane. Compare the results and discuss how the
enfolding of the inner membrane increases surface area through
folding.
The Photosystem I and ATP synthase protein complexes are present

in the stroma. These protein complexes act as spacers between the
sheets of stromal thylakoids.
24
1. Using the figure below, ask learners to compute surface area vs. volume.
2. Draw the table on the board and instruct the learners to write their measurements.
Teacher tip
EVALUATION (60 MINS)
Ask the learners to answer practice questions on the following electronic resources:
Clarify to the learners the

misconception that the appearance of
organelles are static and rigid.
http://www.mcqbiology.com/2013/03/multiple-choice-questions-on_25.html#.Vl7Uq3YrLrc
http://www.uic.edu/classes/bios/bios100/summer2004/samples02.htm
http://www.tutorvista.com/content/science/science-i/fundamental-unit-life/question-answers-1.php
http://www.buzzfeed.com/kellyoakes/the-mitochondria-is-the-powerhouse-of-the-cell#.fajAl0b6o
http://global.oup.com/uk/orc/biosciences/cellbiology/wang/student/mcqs/ch10/
Possible responses to the homework (Source: Campbell et al, 10th Ed.):

They have double membranes and are not part of the endomembrane system.
Their shape is changeable.
They are autonomous (somewhat independent) organelles that grow and occasionally pinch in two,
thereby reproducing themselves.
They are mobile and move around the cell along tracks of the cytoskeleton, a structural network of the
cell.
They contain ribosomes, as well as multiple circular DNA molecules associated with their inner
membranes. The DNA in these organelles programs the synthesis of some organelle proteins on
ribosomes that have been synthesized and assembled there as well.
2. Give out the homework for next meeting.
What are the characteristics shared by these two energy transforming organelles?
Instruct the learners to write an essay on probable reasons for these the shared characteristics of the
mitochondria and the chloroplast. Learners shall submit a handwritten essay on the Endosymbiotic Theory
and how it explains the similarity between the mitochondria and chloroplast.
26
Teacher tip
Check the electronic resources on
Endosymbiotic Theory:
https://www.youtube.com/watch?
v=bBjD4A7R2xU (Endosymbiotic
Theory in plain English)
https://www.youtube.com/watch?v=FQmAnmLZtE
EVALUATION
Learning Competency
Assessment Tool
The learners shall be

able to describe the
following:
Learner
participation
(during lecture)
1. structure and
(STEM_BIO11/12-Iac-2)
Assignment
Examination
Exemplary
Satisfactory
Developing
Learner was able to

answer all the question/
s without referring to
his/her notes
Learner was able to

answer the main question
her notes but was not
able to answer follow-up
question/s
Learner was able to

answer the
questions but he/
she referred to his/
her notes
(1) Learner was not

able to answer the
question/s
requirements
Learner submitted a
Learner submitted a
well written report
but some responses
lack details
(1) Learner did not

submit an
assignment

89.99% correct answers
in the examination
Learner obtained
50% to 69.99%
correct answers in
the examination
Learner obtained
less that 50% correct
answers in the
examination
essay that was
comprehensive and wellwritten
Learner submitted a
well-written essay
some details are
lacking
(1) Learner did not

submit an essay
Learner obtained 90%

to 100% correct
answers in the
examination
Essay Assignment Learner submitted an

essay beyond the
requirements
Beginnning
(2) Learner read

notes of his/her
classmate
(2) Learner
submitted a
partially-finished
assignment
(2) Learner
submitted a
partially-finished
essay
General Biology 1
60 MINS
Mitochondria and Chloroplasts

Content Standards
LESSON OUTLINE
The learners demonstrate an understanding of the structure and function of the
mitochondria and chloroplasts, the organelles involved in energy
Introduction Review of relevant terminologies and
definitions
transformation.
using indigenous or recyclable materials. These models should show the
mitochondria and chloroplasts.
Motivation
Instruction/ Discussion and lecture proper

Delivery
30
The learners describe the structure and function of major and subcellular
organelles (STEM_BIO11/12-Ia-c-2) and distinguish prokaryotic and eukaryotic
cells according to their distinguishing features (STEM_BIO11/12 -Ia-c-3)
Practice
10
Understanding of key concepts using real-life

situations
Drawing (with label) activity
Enrichment Computation of surface area vs volume
Evaluation
Answering practice questions and homework

(1) http://scienceaid.co.uk/biology/biochemistry/atp.html
(2) http://www.britannica.com/list/6-cell-organelles)
(4) http://www.britannica.com/list/6-cell-organelles
(6) http://biology.tutorvista.com/animal-and-plant-cells/chloroplasts.html
(7) ttp://www.nature.com/scitable/topicpage/mitochondria-14053590
Facilitate a review of the following concepts:
Differences between prokaryotic and eukaryotic cells

Definition of an organelle
Differences between membrane-bound organelles and non-membrane-bound organelles
Functions of the different parts of a cell
The endomembrane system
MEMBRANE-BOUND ORGANELLES
NON-MEMBRANE-BOUND ORGANELLES
Nucleus
Ribosomes
Smooth ER
Centrioles
Rough ER
Cytoskeleton
Golgi Apparatus
Vacuoles and Vesicles
Mitochondria
Chloroplast and other plastids
Lysosomes
Peroxisomes
Explain that in eukaryotic cells, the machinery of the cell is compartmentalized into organelles. The compartmentalization of the cell into
membrane-bound organelles:
allows conflicting functions (i.e., synthesis vs. breakdown) and several cellular activities to occur simultaneously without interference from
each other
16
Encourage the learners to look at the cell as both a system and subsystem. They should develop an
Emphasize to the learners that energy transformation is one of the characteristics of life. This refers to
the ability to obtain and use energy. This characterizes the main function of the mitochondria and the
chloroplasts.
MOTIVATION (5 MINS)
Ask the learners how they understand the concept of compartmentalization. Relate the concept to how
the cell is compartmentalized into organelles.
Compare compartmentalization to the division of a house into a receiving room or sala, kitchen, dining
room, comfort rooms, bedrooms, etc.
Teacher tip
Ask the learners why they think a house is divided into several rooms.
A possible response is that partitioning of the house into different parts facilitates the simultaneous
occurrence of several activities without interfering with one another. Also, materials needed for each
activity can be stored at their specific areas. For example, pots and pans are being stored in the kitchen
and not in the bedroom. Beds and pillows are found in the bedroom and not in the toilet/bath.
Explain to the learners that the mitochondria and chloroplasts have a small amount of DNA. Although
most of the proteins of these organelles are imported from the cytosol and are thus programmed by
the nuclear DNA, their DNA programs the synthesis of the proteins made on the organelles ribosomes
(Source: Campbell et al). Compartmentalization separates the DNA material of the nucleus,
mitochondria, and chloroplast.
Ask the learners if they have experienced going to a city/municipal hall and if they have observed that
the Mayor, Vice-Mayor, and the City/Municipal Administrator have separate offices. You can use other
examples such as the University President, VP for Academic Affairs, VP for Finance; Philippine
President, Vice President, Senators, etc.
Compare the nuclear DNA to the Mayor and the mitochondrial DNA and chloroplast DNA to the Vice
Explain to the learner that this is how the

cell is able to allow conflicting functions
(e.g., synthesis vs breakdown) and several
cellular activities to occur simultaneously
without interference from each other.
Mayor. The Mayor runs the city/municipality but the Vice Mayor also performs functions that are
specific to their positions. They need different offices (or compartments) to avoid conflict in their
functions.
Teacher tip
Select a fruit that can be easily peeled like
calamansi or dalandan
Introduce the concept of surface area-volume ratio/relationship to the learners. Show a fruit to the
learners and explain that the outer surface of the fruit is the surface area. Peel the fruit and show them
whats inside, explaining that the inside of the fruit is the volume.
Explain to the learners that surface area (SA) and volume (V) do not increase in the same manner. As an
object increases in size, its volume increases as the cube of its linear dimensions while surface area
increases as the square of its linear dimensions.
Example: If the initial starting point is the same: SA = 2; Volume = 2 (Ratio = 1:1)
A one-step increase will result to: SA = 22 = 4 while V = 23 = 8 (Ratio = 1:2)
Teacher tip
Ask questions to the learners while giving
the lecture.

Explain and discuss the nature and functions of the Adenosine Triphosphate (ATP) to the learners.
Adenosine Triphosphate (ATP)It is the majorenergy currencyof the cell that provides the energy for
most of the energy-consuming activities of the cell. The ATP regulates many biochemical pathways.
Mechanism: When the third phosphate group of ATP is removed by hydrolysis, a substantial amount of
free energyis released.
ATP + H2O ADP + Pi where ADP is adenosine diphosphate and Piis inorganic phosphate
Group the learners into pairs. Ask one to draw the endomembrane system as he/she explains it to his/
her partner. Reshuffle the groupings and repeat until all learners have performed the exercise.
18

the structure of the mitochondria and
chloroplast on the board.
Illustration 1: Energy release in Hydrolysis (Source: (n.d.). Retrieved from http://scienceaid.co.uk/biology/biochemistry/atp.html)
Illustration 2: Chemical Energy and ATP (Source: (n.d.). Retrieved from http://winklebiology.weebly.com/chemical-energyatp.html)
Synthesis of ATP
ADP + Pi ATP + H2O
requires energy: 7.3 kcal/mole
occurs in the cytosol by glycolysis
occurs in mitochondria by cellular respiration

occurs in chloroplasts by photosynthesis
Consumption of ATP
ATP powers most energy-consuming activities of cells, such as:
anabolic (synthesis) reactions, such as:

joining transfer RNAs to amino acids for assembly into proteins
synthesis of nucleoside triphosphates for assembly into DNA and RNA
synthesis of polysaccharides
synthesis of fats
active transport of molecules and ions
conduction of nerve impulses
maintenance of cell volume by osmosis
addition of phosphate groups (phosphorylation) to different proteins (e.g., to alter their activity incell
signaling)
muscle contraction
beating of cilia and flagella (including sperm)
bioluminescence
Extracellular ATP
In mammals, ATP also functions outside of cells. ATP is released in the following examples:
from damaged cells to elicit inflammation andpain

from thecarotid body to signal a shortage of oxygen in the blood
fromtaste receptor cells to trigger action potentials in the sensory nerves leading back to the brain
from the stretched wall of the urinary bladder to signal when the bladder needs emptying
In eukaryotic cells, the mitochondria and chloroplasts are the organelles that convert energy to other
forms which cells can use for their functions.
Discuss the function and structure of the mitochondria.
20
Mitochondria (singular, mitochondrion)Mitochondria are the sites of cellular respiration, the

metabolic process that uses oxygen to drive the generation of ATP by extracting energy from sugars,
fats, and other fuels.
The mitochondria are oval-shaped organelles found in most eukaryotic cells. They are considered to be
the powerhouses of the cell. As the site of cellular respiration, mitochondria serve to transform
molecules such as glucose into an energy molecule known as adenosine triphosphate (ATP). ATP fuels
cellular processes by breaking its high-energy chemical bonds. Mitochondria are most plentiful in cells
that require significant amounts of energy to function, such as liver and muscle cells.
Figure 1: Structure of the Mitochonsdria (Source: (n.d.). Retrieved from http://www.britannica.com/list/
6-cell-organelles)
The mitochondria has two membranes that are similar in composition to the cell membrane:
Outer membraneis a selectively permeable membrane that surrounds the mitochondria. It is the
site of attachment for the respiratory assembly of the electron transport chain and ATP Synthase. It
has integral proteins and pores for transporting molecules just like the cell membrane
Inner membranefolds inward (called cristae) to increase surfaces for cellular metabolism. It
contains ribosomes and the DNA of the mitochondria. The inner membrane creates two enclosed
spaces within the mitochondria:
intermembrane space between the outer membrane and the inner membrane; and
matrix that is enclosed within the inner membrane.
Ask questions to the learners on the structure of the mitochondria. A sample question could be: What
is the importance of the enfolding of the mitochondria? The response would be to increase the surface
area that can be packed into such a small space.
Discuss the purpose of the mitochondrial membranes.
22
As mentioned, the mitochondria has two membranes: the outer and inner mitochondrial membranes.
Outer Membrane
fully surrounds the inner membrane, with a small intermembrane spacein between
has many protein-based pores that are big enough to allow the passage of ions and
molecules as large as a small protein
Inner membrane
has restricted permeability like the plasma membrane
is loaded with proteins involved in electron transport and ATP synthesis
surrounds the mitochondrial matrix, where the citric acid cycle produces the electrons that
travel from one protein complex to the next in the inner membrane. At the end of this
electron transport chain, the final electron acceptor is oxygen, and this ultimately forms
water (H20). At the same time, the electron transport chain produces ATP in a process called
oxidative phosphorylation
During electron transport, the participating protein complexes push protons from the matrix out to the
intermembrane space. This creates a concentration gradient of protons that another protein complex,
calledATP synthase, uses to power synthesis of the energy carrier molecule ATP.
Figure 4: The Electrochemical Proton Gradient and the ATP Synthase (Source: (n.d.). Retrieved from
http://www.nature.com/scitable/topicpage/mitochondria-14053590)
Explain and discuss the structure and functions of the Chloroplasts.
ChloroplastsChloroplasts, which are found in plants and algae, are the sites of photosynthesis. This
process converts solar energy to chemical energy by absorbing sunlight and using it to drive the
synthesis of organic compounds such as sugars from carbon dioxide and water.
The word chloroplast is derived from the Greek word chloros which means green and plastes which
means the one who forms. The chloroplasts are cellular organelles of green plants and some
eukaryotic organisms. These organelles conduct photosynthesis. They absorb sunlight andconvert it
into sugar molecules. They also produce free energy stored in the form of ATP and NADPHthrough
photosynthesis.
Chloroplasts are double membrane-bound organelles and are the sites of photosynthesis. The
22
Teacher tip
Lecture on mitochondrial membranes can

be accessed at (n.d.). Retrieved from
<http://www.nature.com/scitable/
topicpage/mitochondria-14053590>.
chloroplast has a system of three membranes: the outer membrane, the inner membrane, and the
thylakoid system.The outer and the inner membranes of the chloroplast enclose a semi-gel-like fluid
known as the stroma. The stroma makes up much of the volume of the chloroplast. The thylakoid
system floats in the stroma.
Structure of the Chloroplast
Outer membraneThis is a semi-porous membrane and is permeable to small molecules and ions
which diffuse easily. The outer membrane is not permeable to larger proteins.
Intermembrane SpaceThis is usually a thin intermembrane space about 10-20 nanometers and is
present between the outer and the inner membrane of the chloroplast.
Inner membraneThe inner membrane of the chloroplast forms a border to the stroma. It
regulates passage of materials in and out of the chloroplast. In addition to the regulation activity,
fatty acids, lipids and carotenoids are synthesized in the inner chloroplast membrane.
StromaThis is an alkaline, aqueous fluid that is protein-rich and is present within the inner
membrane of the chloroplast. It is the space outside the thylakoid space. The chloroplast DNA,
chloroplast ribosomes, thylakoid system, starch granules, and other proteins are found floating
around the stroma.
Thylakoid System
The thylakoid system is suspended in the stroma. It is a collection of membranous sacks called
thylakoids. Thylakoids are small sacks that are interconnected. The membranes of these thylakoids are
the sites for the light reactions of the photosynthesis to take place. The chlorophyll is found in the
thylakoids. The thylakoids are arranged in stacks known as grana. Each granum contains around 10-20
thylakoids.
The wordthylakoid is derived from the Greek word thylakos which means 'sack'.
Important protein complexes which carry out the light reaction of photosynthesis are embedded in the
membranes of the thylakoids.
The Photosystem I and the Photosystem II are
Teacher tip
the structure of the chloroplast on the
board.
Lecture on structure and functions of the

chloroplast can be accessed at (n.d.).
Retrieved from <http://
biology.tutorvista.com/animal-and-plantcells/chloroplasts.html>.
complexes that harvest light with chlorophyll and carotenoids. They

absorb the light energy and use it to energize the electrons.
The molecules present in the thylakoid membrane use the electrons
that are energized to pump hydrogen ions into the thylakoid space.
This decreases the pH and causes it to becomeacidic in nature. A
large protein complex known as the ATP synthase controls the
concentration gradient of the hydrogen ions in the thylakoid space
to generate ATP energy. The hydrogen ions flow back into the
stroma.
PRACTICE (10 MINS)
Thylakoids areof two types: granal thylakoids and stromal

thylakoids. Granal thylakoids are arranged in the grana. These
circular discs that are about 300-600 nanometers in diameter. The
stromal thylakoids are in contact with the stroma and are in the form
of helicoid sheets.
Group the learners into pairs. Ask one to draw the mitochondria and
label its parts while the other does the same for chloroplast. Once
done, the partners exchange tasks (i.e., the learner that drew the
mitochondria now does the same for the chloroplast).
The granal thylakoids contain only Photosystem II protein complex.

This allows them to stack tightly and form many granal layers with
granal membrane. This structure increases stability and surface area
for the capture of light.
Reproduce these diagrams without the labels and use these for the
class activity.
To demonstrate how folding increases surface area, ask the learners
to trace the edges of the outer membrane with a thread and
measure the length of the thread afterwards. Repeat the same for
the inner membrane. Compare the results and discuss how the
enfolding of the inner membrane increases surface area through
folding.
The Photosystem I and ATP synthase protein complexes are present

in the stroma. These protein complexes act as spacers between the
sheets of stromal thylakoids.
24
1. Using the figure below, ask learners to compute surface area vs. volume.
2. Draw the table on the board and instruct the learners to write their measurements.
Teacher tip
Ask the learners to answer practice questions on the following electronic resources:
Clarify to the learners the

misconception that the appearance of
organelles are static and rigid.
http://www.mcqbiology.com/2013/03/multiple-choice-questions-on_25.html#.Vl7Uq3YrLrc
http://www.uic.edu/classes/bios/bios100/summer2004/samples02.htm
http://www.tutorvista.com/content/science/science-i/fundamental-unit-life/question-answers-1.php
http://www.buzzfeed.com/kellyoakes/the-mitochondria-is-the-powerhouse-of-the-cell#.fajAl0b6o
http://global.oup.com/uk/orc/biosciences/cellbiology/wang/student/mcqs/ch10/
Possible responses to the homework (Source: Campbell et al, 10th Ed.):

They have double membranes and are not part of the endomembrane system.
Their shape is changeable.
They are autonomous (somewhat independent) organelles that grow and occasionally pinch in two,
thereby reproducing themselves.
They are mobile and move around the cell along tracks of the cytoskeleton, a structural network of the
cell.
They contain ribosomes, as well as multiple circular DNA molecules associated with their inner
membranes. The DNA in these organelles programs the synthesis of some organelle proteins on
ribosomes that have been synthesized and assembled there as well.
2. Give out the homework for next meeting.
What are the characteristics shared by these two energy transforming organelles?
Instruct the learners to write an essay on probable reasons for these the shared characteristics of the
mitochondria and the chloroplast. Learners shall submit a handwritten essay on the Endosymbiotic Theory
and how it explains the similarity between the mitochondria and chloroplast.
26
Teacher tip
Check the electronic resources on
Endosymbiotic Theory:
https://www.youtube.com/watch?
v=bBjD4A7R2xU (Endosymbiotic
Theory in plain English)
https://www.youtube.com/watch?v=FQmAnmLZtE
EVALUATION
Learning Competency
Assessment Tool
The learners shall be

able to describe the
following:
Learner
participation
(during lecture)
1. structure and
(STEM_BIO11/12-Iac-2)
Assignment
Examination
Exemplary
Satisfactory
Developing
Learner was able to

answer all the question/
s without referring to
his/her notes
Learner was able to

answer the main question
her notes but was not
able to answer follow-up
question/s
Learner was able to

answer the
questions but he/
she referred to his/
her notes
(1) Learner was not

able to answer the
question/s
requirements
Learner submitted a
Learner submitted a
well written report
but some responses
lack details
(1) Learner did not

submit an
assignment

89.99% correct answers
in the examination
Learner obtained
50% to 69.99%
correct answers in
the examination
Learner obtained
less that 50% correct
answers in the
examination
essay that was
comprehensive and wellwritten
Learner submitted a
well-written essay
some details are
lacking
(1) Learner did not

submit an essay
Learner obtained 90%

to 100% correct
answers in the
examination
Essay Assignment Learner submitted an

essay beyond the
requirements
Beginnning
(2) Learner read

notes of his/her
classmate
(2) Learner
submitted a
partially-finished
assignment
(2) Learner
submitted a
partially-finished
essay
General Biology 1
180 MINS
Structure and Functions of Animal Tissues

and Cell Modification
LESSON OUTLINE
Content Standard
The learners demonstrate an understanding of animal tissues and cell
modification.
Introduction Communicating learning objectives to the

learners.
Performance Standard
Motivation
The learners shall be able to construct a three-dimensional model of the animal
tissue by using recyclable or indigenous materials.
The learners:

10
Instruction/ Review on the Hierarchy of Biological

Organisation and PTSF; Lesson on Animal
Delivery
95
Practice
Class Activity: Reporting on structure and

function of animal tissue or showing of
infomercial on diseases.
60
Evaluation
Class Quiz
10
Tissues and on Cell Modfication
classify different cell types (plant/animal tissue) and specify the functions of
each (STEM_BIO11/12-Ia-c-4)
describe some cell modifications that lead to adaptation to carry out
specialized functions (e.g., microvilli, root hair) (STEM_BIO11/12-Ia-c-5)
Class Activity: Pinoy Henyo Classroom

Edition
Materials
present a five-minute report on how the structures of different animal

tissues define their function or show a two-minute infomercial about a
disease that is caused by animal tissue malfunction;
provide insights, offer constructive feedback, and note areas of
improvement on their classmates reports or infomercial
microscopes, LCD Projector (if available), laptop or computer

(if available), manila paper, cartolina, photos, images, or
illustrations of different types of tissues, drawing materials
(e.g. pens, pencils, paper, color pencils, etc.)
(1) Reece JB, U. L., (2010). Campbell Biology 10th. San Francisco (CA).
28
Introduce the following learning objectives by flashing these on the board:
classify different cell types (plant/animal tissue) and specify the functions of each (STEM_BIO11/12Ia-c-4)
describe some cell modifications that lead to adaptation to carry out specialized functions (e.g.,
microvilli, root hair) (STEM_BIO11/12-Ia-c-5)
Ask the learners to work in pairs and write the learning objectives using their own words.
MOTIVATION (10 MINS)

PINOY HENYO CLASSROOM EDITION
Divide the class into two groups.
Explain to the learners that instead of having the typical one-on-one Pinoy Henyo, only one
representative from each group shall be asked to go to the front and have the mystery word card on
his/her forehead. Only three words shall be allowed from the groups: Oo, Hindi, or Pwede.
Violation of the rules of the game (e.g., communicating the mystery word to the guesser) shall merit
corresponding penalties or disqualification. Assign three representatives per group to guess the
mystery words. Each guesser shall be given one minute and 30 seconds.
At the end of the activity, ask one or two learners what they think the learning objectives of the lesson
will be. Immediately proceed with the Introduction.
Teacher tip
For this particular lesson, start with the
Motivation first (i.e., class activity on Pinoy
Henyo Classroom Edition). After the game,
proceed to the Introduction by
communicating the learning objectives to
the learners.
For the part when the learners have to state
the learning objectives using their own
words, ask the learners to face their
seatmates and work in pairs. If the learners
are more comfortable in stating the learning
objectives in Tagalog or In their local
dialect, ask them to do so.
Teacher tip
Prior to this lesson, assign a reading
material or chapter for this topic. This shall
aid in the facilitation of the class activity.
In choosing the mystery words for the
game, do not limit yourself with the four
types of animal tissues. You may choose
terms that describe the tissue type or even
body parts wherein the tissues are located.
You may also include diseases that are
caused by certain malfunctions on the
tissues.
Make sure to mention the chosen mystery
words in the discussion. This shall help the
learners to understand the connection of
the game with the lesson.
Check how the class behaves during the
activity. If the learners get rowdy, you may
choose to stop the game. Make sure to
warn the learners of the consequences first
before the start of the activity.

Teacher tip
Facilitate a five-minute review on the Hierarchy of Biological Organization and on the concept of form
fits function, the unifying theme in Biology.
Review on Hierarchy of Biological Organization
Do not use too much time for the review.

Just make sure to guide or lead the learners
in remembering past lessons. Provide clues
if necessary.
1. Discuss that new properties arise with each step upward the hierarchy of life. These are called
emergent properties.
2. Ask the class what the levels of biological organization are. The learners should be able to answer
this since this is just a review. In case the class does not respond to the question, you may facilitate
the discussion by mentioning the first level of the hierarchy.
3. Start with the cell since it is the most basic unit of life that shows all life properties.
cells
tissue organ
organ system
multicellular organism
Illustrate this by showing photos of the actual hierarchy using animals that are endemic in the
Philippines (e.g., pilandok, dugong, and cloud rat).
Review on the unifying theme in Biology: form fits function
1. Ask the class what the relation of form (structure) to function and vice versa is
2. Ask for examples of versaingit of life that shows all life properthe torpedo shape of the body of
dolphins (mammals with fishlike characteristics) and the bone structure and wing shape of birds in
relation to flying.
30
Teacher tip
For the review on form fits function, if the
class does not respond well, start giving
your own examples for the students to
figure out this unifying theme.
Make sure to relate structure to function.
Mention the role of fossils in determining
the habits of extinct animals. By doing this,
it shall establish a strong connection
between form and function and shall give
relevance on the study of this connection in
Biology. After this, you may now proceed to
the new topic on animal tissues.
Facilitate a class activity (i.e., observation of cells under a microscope) to illustrate that animals are
made up of cells. This shall be the foundation of the definition of and discussion on animal tissues. The
whole activity and discussion shall last for 90 minutes.
If microscopes are available for this activity, set up the equipment and the slides that were prepared
prior to the activity. Each slide should show one type of tissue (i.e., epithelial tissue, connective tissue,
muscle tissue, and nervous tissue). Make sure that the labels are covered because the learners will be
asked to name the tissues based on their observations during the discussion.
If there are no microscopes available for the activity, prepare cut-out images, photos, or illustrations
that show the different types of tissues (i.e., epithelial tissue, connective tissue, muscle tissue, and
nervous tissue). Make sure that the images, photos, or illustrations are not labeled because the learners
will be asked to name them.
Also, do not immediately identify the type of tissue based on the descriptions that you will be
presenting to the class. The learners will be asked to identify which among the slides under the
microscope or which image, photo, or illustration matches the description of the structure and function
that will be given during the discussion.
After the class activity, proceed with the actual lecture. If a computer, laptop, or projector is available,
show a PowerPoint presentation that shows the description and function of tissues. If there is no
available equipment, you may use flash cards or manila paper where description of structure and
function of the different tissue types are written down. Ask the learners which among the microscope
slides, image, photo, or illustration fits the given information on description and function. After the
learners responses, you can flash or show the next slide which shall reveal the image of the specimen
with the corresponding label or type of tissue.
Epithelial TissueThis type of tissue is commonly seen outside the body as coverings or as linings of
organs and cavities. Epithelial tissues are characterized by closely-joined cells with tight junctions (i.e., a
type of cell modification). Being tightly packed, tight junctions serve as barriers for pathogens,
mechanical injuries, and fluid loss.
Teacher tip
If microscopes are available for this activity,
allot 20-30 minutes for the observation of
cells. If microscopes are not available, allot
only 10-15 minutes.
Prior to the activity, prepare the slides that
will be put under the microscopes. The
slides shall contain the different types of
tissue. Make sure to focus the slides so that
the learners can observe them clearly.
Give the learners enough time to observe
the specimens and then ask them to draw
on their notebooks what they were able to
observe under the microscopes. Encourage
the learners to write down the description
and function of the specific tissue type as
you go through the discussion.
If microscopes are not available and you
have shown photos, images, or illustrations
instead, ask the learners to draw them on
their notebooks and encourage them to
write down the description and function of
the specific tissue type as you go through
the discussion.
Teacher tip
Prepare the lecture in such a way that you
do not immediately reveal the label of the
images or the terms that are being
described. The learners should first be
asked to identify the images or slides that fit
the description of the structures and
functions. This will make the students more
engaged in the discussion. Always remind
the learners to take down notes while you
flash information for each tissue type.
Teacher tip
Cells that make up epithelial tissues can have distinct arrangements:
cuboidalfor secretion
simple columnarbrick-shaped cells; for secretion and active absorption
simple squamousplate-like cells; for exchange of material through diffusion
stratified squamousmultilayered and regenerates quickly; for protection
pseudo-stratified columnarsingle layer of cells; may just look stacked because of varying height;
for lining of respiratory tract; usually lined with cilia (i.e., a type of cell modification that sweeps the
mucus).
Figure 1: Epithelial Tissue (Source: Reece JB, U. L. (2010). Campbell Biology 10th. San Francisco (CA):.)
32
Take note that the part on cell modifications

is incorporated in the discussion on the
structure of the respective cells that make
up the tissue that is being discussed. Give
emphasis on the differences on the features
of the cells that make up the tissue type.
For examples or illustrations of the different
types of tissues, it is better to use an animal
that is endemic in the Philippines or in your
specific region so that the learners can
relate more in the discussion.
Connective TissueThese tissues are composed of the

following:
BLOOD made up of plasma (i.e., liquid extracellular matrix);
contains water, salts, and dissolved proteins; erythrocytes that
carry oxygen (RBC), leukocytes for defense (WBC), and platelets
for blood clotting.
CONNECTIVE TISSUE PROPER (CTP)made up of loose
connective tissue that is found in the skin and fibrous connective
tissue that is made up of collagenous fibers found in tendons
and ligaments. Adipose tissues are also examples of loose
connective tissues that store fats which functions to insulate the
body and store energy.
CARTILAGE characterized by collagenous fibers embedded
in chondroitin sulfate. Chondrocytes are the cells that secrete
collagen and chondroitin sulfate. Cartilage functions as cushion
between bones.
BONE mineralized connective tissue made by bone-forming
cells called osteoblasts which deposit collagen. The matrix of
collagen is combined with calcium, magnesium, and phosphate
ions to make the bone hard. Blood vessesl and nerves are
found at a central canal surrounded by concentric circles of
osteons.
Figure 2: Connective Tissue (Source: Reece JB, U. L. (2010).

Campbell Biology 10th. San Francisco (CA):.)
Muscle TissueThese tissues are composed of long cells called muscle

fibers that allow the body to move voluntary or involuntary. Movement
of muscles is a response to signals coming from nerve cells. In
vertebrates, these muscles can be categorized into the following:
skeletalstriated; voluntary movements
cardiacstriated with intercalated disk for synchronized heart
contraction; involuntary
smoothnot striated; involuntary
Figure 3: Muscle Tissue (Source: Reece JB, U. L. (2010). Campbell
Biology 10th. San Francisco (CA):.)
Nervous TissueThese tissues are composed of nerve cells called
neurons and glial cells that function as support cells. These neurons
sense stimuli and transmit electrical signals throughout the animal body.
Neurons connect to other neurons to send signals. The dendrite is the
part of the neuron that receives impulses from other neurons while the
axon is the part where the impulse is transmitted to other neurons.
Figure 4: Neurons and Glial Cells (Source: Reece JB, U. L. (2010). Campbell
Biology 10th. San Francisco (CA):.)
PRACTICE (60 MINS)

Divide the class into six groups. Four groups will be reporting on Animal Tissues while two groups will
be creating an infomercial on diseases caused by the malfunction of tissue types. Each infomercial
group shall cover two tissue types.
Each group will be given five minutes to report or show their infomercial. At the end of each
presentation, facilitate a five-minute critiquing of the presentation. Make sure to get feedbacks from
the learners and clarify misconceptions from the reports. The report or the infomercial on diseases shall
not be graded. These will be a kind of formative assessment.Group the learners into pairs. Ask one to
draw the mitochondria and label its parts while the other does the same for chloroplast. Once done,
the partners exchange tasks (i.e., the learner that drew the mitochondria now does the same for the
chloroplast).
Ask the learners to group themselves in pairs or in groups of threes. This will allow the learners to
discuss and decide among themselves. However, if a learner chooses to do this activity on his or her
own, he or she should be allowed to do so.
Ask the learners to briefly and clearly answer the following questions:
What is the importance of having a tissue level in the hierarchy of biological organization? (2 points)
What do the varying shapes and arrangement of epithelial tissue suggests? (2 points)
What is the general function of connective tissues? What function is common to all types of
connective tissues? (1 point)
Why are there voluntary and involuntary muscle tissue functions? (2 points)
What is the importance of glial cells in nervous tissues? (1 point)
Identify two cell modifications and describe their respective functions. (2 points)
Teacher tip
Group the learners before starting the
lesson. The reporting may be done the day
after finishing the discussion on Animal
Tissue Structure, Function, and Cell
Modification.
The reports may be presented using a table
which contains columns for tissue type, cell
structures that characterize the tissue, part
of the body where the tissue is located,
function, and importance.
Teacher tip
Assess if the learners are ready to answer
this individually. If they are not yet ready,
this activity can be done in pairs or in
groups of threes. Make sure that you
provide enough time for the group to
discuss their responses. Remind the learners
to answer briefly and clearly.
If you are not comfortable with this time of
exam, a multiple-choice type of evaluation
may also be prepared.
After getting the responses, you may get
feedback from the learners to see if all
members of each group helped or
participated in their small discussions to
answer the short quiz. You may ask learners
to rate the members of their group.
General Biology 1
90 MINS
Cell Cycle and Cell Division

Content Standard
The learners demonstrate an understanding of the cell cycle and cell division
(i.e., mitosis and meiosis).
LESSON OUTLINE
Introduction Presentation of a simplified life cycle of a
human being or plant
Motivation
The learners shall be able to construct a three-dimensional model of the stages
or phases involved in the cell cycle using indigenous or recyclable materials.
Instruction/
The learners shall put emphasis on the identification of possible errors that may Delivery
happen during these stages.
The learners:
characterize the phases of the cell cycle and their control points (STEM_BIO11/12Id-f-6)
describe the stages of mitosis and meiosis given 2n=6 (STEM_BIO11/12-Id-f-7)
discuss crossing over and recombination in meiosis (STEM_BIO11/12-Id-f-8)
explain the significance or applications of mitosis/meiosis (STEM_BIO11/12-Id-f-9)
identify disorders and diseases that result from the malfunction of the cell during
the cell cycle (STEM_BIO11/12-Id-f-10)

Identify and differentiate the phases of the cell cycle and their control
points
describe and differentiate the stages of mitosis and meiosis given 2n=6
discuss and demonstrate crossing over and recombination in meiosis
explain the significance and applications of mitosis and meiosis
construct a diagram of the various stages of mitosis and meiosis
identify disorders and diseases that result from malfunctions in the cell
during the cell cycle
Practice
Video presentation of Cell Cycle and Cell

Division
60
Class activities or games such as Amazing

Race or Interphase, Mitosis, or Meiosis
Puzzle
10
and animal gametogenesis; Microscopic

examination of an onion root tip
Written or oral examination
Materials
photos of the life cycle or stages of eukaryotic organisms,

yarns of different thickness, cords, beads, coins, pens
(1) Becker, W.M. (2000). The World of the Cell. Addison Wesley Longman
Inc., USA
(2) Mader, S.S. (2011).Biology 10th Ed. Mac Graw Hill Education, USA.
36
Lecture-discussion on the cell through the

use of a PowerPoint presentation, video, or
cell diagram on a Manila paper;
Demonstration of the processes inside the
cell using model materials (e.g., beads,
cords, yarn with different thickness, coins,
etc.); or, Summary of learners responses to
questions regarding the video on Cell Cycle
and Cell Division
Enrichment Video presentation or introduction on plant
Evaluation
Teacher tip
Introduce a simplified life cycle of a human being or plant. Let the learners identify the changes
throughout the different stages and how these organisms grow and develop.
Explain to the learners that these eukaryotic

organisms follow a complex sequence of
events by which their cells grow and divide.
This sequence of events is known as the Cell
Cycle.
You can show diagrams or illustrations that
demonstrate the growth or increase in the
number of organisms.
Figure 1: Life Cycle of Man and Higher Plants (Source: (n.d.). Retrieved from http://
www.vcbio.science.ru.nl/en/virtuallessons/cellcycle/postmeio/)
MOTIVATION (5 MINS)
1. Play the video on Cell Cycle and Cell Division. This video can be accessed at http://
www.youtube.com/watch?v=Q6ucKWIIFmg.Divide the class into two groups.
2. Show diagrams of cell division in multicellular or eukaryotic organisms to the class.
38
Teacher tip
You can download the video prior to this
session or if internet connection is available
during class, you can just make use of the
hyperlink to play the video. To access the
video through the hyperlink, simply hold the
Control (Ctrl) Key on the keyboard and click
on the hyperlink.
You should ask the learners thoughtprovoking questions about the video and
relate it to the lesson.
Teacher tip
Facilitate a lecture-discussion on the general concepts of cell division.

Cell Divisioninvolves the distribution of identical genetic material or DNA to two daughter cells.
What is most remarkable is the fidelity with which the DNA is passed along, without dilution or error,
from one generation to the next. Cell Division functions in reproduction, growth, and repair.
Core Concepts:
All organisms consist of cells and arise from preexisting cells.

Mitosis is the process by which new cells are generated.
Meiosis is the process by which gametes are generated for reproduction.
The Cell Cycle represents all phases in the life of a cell.
DNA replication (S phase) must precede mitosis so that all daughter cells receive the same
complement of chromosomes as the parent cell.
The gap phases separate mitosis from S phase. This is the time when molecular signals mediate the
switch in cellular activity.
Mitosis involves the separation of copied chromosomes into separate cells.
Unregulated cell division can lead to cancer.
Cell cycle checkpoints normally ensure that DNA replication and mitosis occur only when conditions
are favorable and the process is working correctly.
Mutations in genes that encode cell cycle proteins can lead to unregulated growth, resulting in
tumor formation and ultimately invasion of cancerous cells to other organs.
The Cell Cycle control system is driven by a built-in clock that can be adjusted by external stimuli (i.e.,
chemical messages).
Checkpointa critical control point in the Cell Cycle where stop and go-ahead signals can regulate
the cell cycle.
Animal cells have built-in stop signals that halt the cell cycles and checkpoints until
overridden by go-ahead signals.
Three major checkpoints are found in the G1, G2, and M phases of the Cell Cycle.
38
Note the learners responses to questions

about the video compared to the expected
responses. The expected responses are the
concepts listed in the Instruction / Delivery
part.
The G1 Checkpointthe Restriction Point
The G1 checkpoint ensures that the cell is large enough to divide and that enough nutrients are available to support the
resulting daughter cells.
If a cell receives a go-ahead signal at the G1 checkpoint, it will usually continue with the Cell Cycle.
If the cell does not receive the go-ahead signal, it will exit the Cell Cycle and switch to a non-dividing state called G0.
Most cells in the human body are in the G0 phase.
The G2 Checkpointensures that DNA replication in S phase has been successfully completed.
The Metaphase Checkpointensures that all of the chromosomes are attached to the mitotic spindle by a kinetochore.
Kinasea protein which activates or deactivates another protein by phosphorylating them. Kinases give the go-ahead signals at the
G1 and G2 checkpoints. The kinases that drive these checkpoints must themselves be activated.
The activating molecule is acyclin,a protein that derives its name from its cyclically fluctuating concentration in the cell.
Because of this requirement, these kinases are calledcyclin-dependent kinases orCDKs.
Cyclins accumulateduring the G1, S, and G2 phases of the Cell Cycle.
By theG2 checkpoint, enough cyclin is available to form MPF complexes (aggregations of CDK and cyclin) which initiate
mitosis.
MPF functions by phosphorylating key proteins in the mitotic sequence.
Later in mitosis, MPF switches itself off by initiating a process which leads to the destruction of cyclin.
CDK, the non-cyclin part of MPF, persists in the cell as an inactive form until it associates with new cyclin molecules
synthesized during the interphase of the next round of the Cell Cycle.
Discuss the stages of mitosis and meiosis.

Mitosis (apparent division)is nuclear division; the process by which the nucleus divides to produce two new nuclei. Mitosis results in two
daughter cells that are genetically identical to each other and to the parental cell from which they came.
Cytokinesisis the division of the cytoplasm. Both mitosis and cytokinesis last for around one to two hours.
Prophaseis the preparatory stage, During prophase, centrioles move toward opposite sides of the nucleus.
The initially indistinct chromosomes begin to condense into visible threads.

Chromosomes first become visible during early prophase as long, thin, and
intertwined filaments but by late prophase, chromosomes are more compacted and
can be clearly discerned as much shorter and rod-like structures.
As the chromosomes become more distinct, the nucleoli also become more
distinct. By the end of prophase, the nucleoli become less distinct, often
disappearing altogether.
Metaphaseis when chromosomes become arranged so that their centromeres become aligned in
one place, halfway between the two spindle poles. The long axes of the chromosomes are 90 degrees
to the spindle axis. The plane of alignment is called the metaphase plate.
Anaphaseis initiated by the separation of sister chromatids at their junction point at the centromere.
The daughter chromosomes then move toward the poles.
Telophaseis when daughter chromosomes complete their migration to the poles. The two sets of
progeny chromosomes are assembled into two-groups at opposite ends of the cell. The chromosomes
uncoil and assume their extended form during interphase. A nuclear membrane then forms around
each chromosome group and the spindle microtubules disappear. Soon, the nucleolus reforms.
Meiosisreduces the amount of genetic information. While mitosis in diploid cells produces
daughter cells with a full diploid complement, meiosis produces haploid gametes or spores with only
one set of chromosomes. During sexual reproduction, gametes combine in fertilization to reconstitute
the diploid complement found in parental cells. The process involves two successive divisions of a
diploid nucleus.
First Meiotic Division
The first meiotic division results in reducing the number of chromosomes (reduction division). In most
cases, the division is accompanied by cytokinesis.
40
Teacher tip
You may show diagrams or a video
demonstrating animal and plant mitosis. The
video can be accessed at http://
www.vcbio.science.ru.nl/en/virtuallessons/
mitostage/
Prophase Ihas been subdivided into five substages: leptonema, zygonema, pachynema, diplonema, and diakinesis.
LeptonemaReplicated chromosomes have coiled and are already visible. The number of chromosomes present is the same
as the number in the diploid cell.
ZygonemaHomologue chromosomes begin to pair and twist around each other in a highly specific manner. The pairing is
called synapsis. And because the pair consists of four chromatids it is referred to as bivalent tetrad.
PachynemaChromosomes become much shorter and thicker. A form of physical exchange between homologues takes
place at specific regions. The process of physical exchange of a chromosome region is called crossing-over. Through the
mechanism of crossing-over, the parts of the homologous chromosomes are recombined (genetic recombination).
DiplonemaThe two pairs of sister chromatids begin to separate from each other. It is at this point where crossing-over is
shown to have taken place. The area of contact between two non-sister chromatids, called chiasma, become evident.
DiakinesisThe four chromatids of each tetrad are even more condensed and the chiasma often terminalize or move down
the chromatids to the ends. This delays the separation of homologous chromosomes.
In addition, the nucleoli disappear, and the nuclear membrane begins to break down.
Metaphase IThe spindle apparatus is completely formed and the microtubules are attached to the centromere regions of the homologues.
The synapsed tetrads are found aligned at the metaphase plate (the equatorial plane of the cell) instead of only replicated chromosomes.
Anaphase IChromosomes in each tetrad separate and migrate toward the opposite poles. The sister chromatids (dyads) remain attached at
their respective centromere regions.
Telophase IThe dyads complete their migration to the poles. New nuclear membranes may form. In most species, cytokinesis follows,
producing two daughter cells. Each has a nucleus containing only one set of chromosomes (haploid level) in a replicated form.
Second Meiotic Division
The events in the second meiotic division are quite similar to mitotic division. The difference lies, however, in the number of chromosomes that
each daughter cell receives. While the original chromosome number is maintained in mitosis, the number is reduced to half in meiosis.
Prophase IIThe dyads contract.
Metaphase IIThe centromeres are directed to the equatorial plate and then divide.
Anaphase IIThe sister chromatids (monads) move away from each other and migrate to the opposite poles of the spindle fiber.
Telophase IIThe monads are at the poles, forming two groups of chromosomes. A nuclear membrane forms around each set of chromosomes
and cytokinesis follows. The chromosomes uncoil and extend.
CytokinesisThe telophase stage of mitosis is accompanied by cytokinesis. The two nuclei are
compartmentalized into separate daughter cells and complete the mitotic cell division process. In
animal cells, cytokinesis occurs by the formation of a constriction in the middle of the cell until two
daughter cells are formed. The constriction is often called cleavage, or cell furrow. However, in most
plant cells this constriction is not evident. Instead, a new cell membrane and cell wall are assembled
between the two nuclei to form a cell plate. Each side of the cell plate is coated with a cell wall that
eventually forms the two progeny cells.
Meiosis
Teacher tip
You can show a tabular comparison
between mitosis and meiosis to point the
significance of the two types of division.
Divide the class into two groups and ask
them about their opinions on the
applications of mitosis and meiosis.
The following could be possible responses:
Mitosis
1. Requires two nuclear divisions
1. Requires one nuclear division
2. Chromosomes synapse and cross

over
2. Chromosomes do not synapse nor cross

over
3. Centromeres survive Anaphase I
3. Centromeres dissolve in mitotic anaphase
4. Halves chromosome number
4. Preserves chromosome number
5. Produces four daughter nuclei
5. Produces two daughter nuclei
6. Produces daughter cells genetically

different from parent and each other
6. Produces daughter cells genetically

identical to parent and to each other
7. Used only for sexual reproduction
7. Used for asexual reproduction and

growth
Table 1: Comparison of Mitosis and Meiosis (Source: http://courses.washington.edu/bot113/spring/

WebReadings/PdfReadings/TABLE_COMPARING_MITOSIS_AND.pdf)
42
Significance of mitosis for sexual

reproduction: Mitosis is important for
sexual reproduction indirectly. It allows the
sexually reproducing organism to grow and
develop from a single cell into a sexually
mature individual. This allows organisms to
continue to reproduce through the
generations.
Significance of Meiosis and Chromosome
Number: Chromosomes are the cell's way
of neatly arranging long strands of DNA.
Non-sex cells have two sets of
chromosomes, one set from each parent.
Meiosis makes sex cells with only one set of
chromosomes. For example, human cells
have 46 chromosomes, with the exception
of sperm and eggs, which contain only 23
chromosomes each. When a sperm cell
fertilizes an egg, the 23 chromosomes from
each sex cell combine to make a zygote, a
new cell with 46 chromosomes. The zygote
is the first cell in a new individual.
Meiosis I compared to Mitosis
Meiosis II compared to Mitosis
Meiosis I
Mitosis
Meiosis II
Mitosis
Prophase I
Prophase
Prophase II
Prophase
Pairing of homologous
chromosomes
No pairing of
chromosomes
No pairing of
chromosomes
No pairing of
chromosomes
Metaphase I
Metaphase
Metaphase II
Metaphase
Bivalents at metaphase
plate
Duplicated
chromosomes at
metaphase plate
Haploid number of
duplicated
chromosomes at
metaphase plate
Diploid number
of duplicated
chromosomes at
metaphase plate
Anaphase I
Anaphase
Anaphase II
Anaphase
Homologues of each
bivalent separate and
duplicated
chromosomes move to
poles
Sister chromatids
separate, becoming
daughter
chromosomes that
move to the poles
Sister chromatids
separate, becoming
daughter
chromosomes that
move to the poles
Sister chromatids
separate
becoming
daughter
chromosomes
that move to the
poles
Telophase I
Telophase
Telophase II
Telophase
Two haploid daughter

cells not identical to the
parent cell
Two diploid
daughter cells,
identical to the
parent cell
Four haploid
daughter cells not
genetically identical
Two diploid
daughter cells,
identical to the
parent cell
Table 2: Meiosis compared to Mitosis

Facilitate a discussion on disorders and diseases that result from the malfunction of the cell during the
cell cycle. Present some diagrams or illustrations on some errors in mitosis and allow the learners to
predict possible outcomes, diseases, or disorders that may happen:
incorrect DNA copy (e.g., cancer)

chromosomes are attached to string-like spindles and begin to move to the middle of the cell (e.g.,
Down Syndrome, Alzheimers, and Leukemia)
Teacher tip
Significance of Meiosis for Diversity:
One of the benefits of sexual reproduction
is the diversity it produces within a
population. That variety is a direct product
of meiosis. Every sex cell made from meiosis
has a unique combination of chromosomes.
This means that no two sperm or egg cells
are genetically identical. Every fertilization
event produces new combinations of traits.
This is why siblings share DNA with parents
and each other, but are not identical to one
another.
Teacher tip
You may show a video that demonstrates
how crossing over and recombination of
chromosomes occur. The video can be
accessed at http://
highered.mheducation.com/sites/
9834092339/student_view0/chapter11/
meiosis_with_crossing_over.html.s
Other chromosome abnormalities:
arise from errors in meiosis, usually meiosis I;

occur more often duringegg formation(90% of the time) than during sperm formation;
become more frequent as a woman ages.
Aneuploidyis the gain or loss of whole chromosomes. It is the most common chromosome
abnormality. It is caused bynon-disjunction, the failure of chromosomes to correctly separate:
homologues during meiosis I or
sister chromatids during meiosis II
PRACTICE (10 MINS)

Facilitate games like Amazing Race, Interphase/Mitosis/Meiosis Puzzle in the class.
1. The Amazing Race follows a series of stations or stages with challenges that the learners have to
accomplish. Divide the class into groups after the discussion. The number of groups will depend on the
number of stages or phases in the process (i.e., interphase, mitosis, or meiosis).
Teacher tip
2. The groups will race to accomplish the tasks in five stations. In each station, the learners will assemble
given materials to illustrate stages or phases of events in the specific process (i.e., interphase, mitosis,
or meiosis).
Encourage the learners to actively

participate in the challenge. You may
give extra points to those who will
finish first.
ENRICHMENT (5 MINS)
A number of good videos have the

stages or phases made into a rap or a
song. One such example is the video
entitled Cell Division Song Spongebob
that can be accessed at
http://www.youtube.com/watch?
v=9nsRufogdoI. Encourage each group
to brainstorm and point out their
perceptions of the videos.
1. Instruct the learners to watch additional videos on cell division.

2. Introduce animal and plant gametogenesis to the learners in order for them to appreciate the
significance of cell division.
3. Facilitate microscopic examination of onion root tip.
EVALUATION (5 MINS)
Facilitate the accomplishment of a self-assessment checklist.
A video on animal and plant

gametogenesis can be accessed at
http://csls-text.c.u-tokyo.ac.jp/active/
12_05.html.
44
ADDITIONAL RESOURCES:
Books:
1. Raven, P. a. (2001). Biology 6th Ed. The McGraw Hill Company, USA
2. Reece, J. B. (2013). Campbell Biology, 10th Ed. Pearson Education, Inc. United States of America.
Electronic Resources:
3. (n.d.). Retrieved from Bright Hub Education: http://www.brighthubeducation.com/middle-school-science-lessons/94267-three-activities-forteaching-cell-cycles/#
4. (n.d.). Retrieved from http://www.uic.edu/classes/bios/bios100/lecturesf04am/lect16.htm
5. (n.d.). Retrieved from MH Education: http://highered.mheducation.com/sites/9834092339/student_view0/chapter11/
meiosis_with_crossing_over.html
6. (n.d.). Retrieved from http://www.vcbio.science.ru.nl/en/virtuallessons/meiostage/
7. (n.d.). Retrieved from http://csls-text.c.u-tokyo.ac.jp/active/12_05.html
8. (n.d.). Retrieved from http://education.seattlepi.com/biological-significance-mitosis-meiosis-sexual-reproduction-5259.htm
General Biology 1
480 MINS
Transport Mechanisms Pt.1
LESSON OUTLINE
Content Standards
The learners demonstrate an understanding of Transport Mechanisms:
Introduction Visualization of the plasma membrane and its
Simple Diffusion, Facilitated Transport, Active Transport, and Bulk/Vesicular

Transport
Motivation
Simple group activity and brief reporting
The learners shall be able to construct a cell membrane model from indigenous Instruction/ Discussion and lecture proper
Delivery
or recyclable materials.
The learners:
Practice
describe the structural components of the cell membrane

(STEM_BIO11/12Ig-h-11)
relate the structure and composition of the cell membrane to its function
explain transport mechanisms in cells (diffusion, osmosis, facilitated
transport, active transport) (STEM_BIO11/12Ig-h-13)
differentiate exocytosis and endocytosis (STEM_BIO11/12-Ig-h-14)

30
functions
60
120
45
Answering practice/guide questions
Enrichment Essay and concept map writing

Evaluation
Designing a model of a plasma

membrane using recyclable or
indigenous materials
45
180
Materials
pen, paper, salt, water, recycled or indigenous materials
describe and compare diffusion, osmosis, facilitated transport and active

transport
explain factors that affect the rate of diffusion across a cell membrane
predict the effects of hypertonic, isotonic, and hypotonic environments on
osmosis in animal cells
differentiate endocytosis (phagocytosis and pinocytosis) and exocytosis
46
Resources
(1) Campbell, N. J. (n.d.).

(2) Campbell, N. e. (2008). Biology 8th edition. Pearson International
Edition. Pearson/Benjamin.
(3) Freeman, S. (2011). Biological Science 4th edition International Edition.
Benjamin Cummings Publishing.
(4) Hickman, C. L. (2011). Integrated Principles of Zoology 15th edition.
McGraw Hill Co., Inc.
1. Before this lesson, ask the learners to read about the topic on transport of materials across
membranes.
2. Introduce the topic by providing the learners with background information.
In order for the cell to stay alive, it must meet the characteristics of life which include taking
nutrients in and eliminating wastes and other by-products of metabolism. Several mechanisms allow
cells to carry out these processes. All of the cells activities are in one way or another tied to the
membrane that separates its interior from the environment.
3. Ask the learners how they understand and visualize a plasma membrane and what characteristics
are essential for it to perform its function.
4. Ask the learners to identify the different mechanisms on how materials are transported in and out of
the cell.
1. Divide the learners into groups and ask them the following question: What comes to your mind
when you see a 20 year old man who is 7.5 ft. tall and 3.5 ft. tall man of the same age? Among
their respective groups, let the learners discuss the similarities and differences between the two.
(Hint: Give students a clue by giving them the giant and pygmy as examples).
2. Ask a representative from each group to report the result of their discussion to the whole class.
3. Before the start of the lesson on diffusion, spray an air freshener in one corner of the room and ask
the learners to raise their hands if they have smelled the scent of the spray.
4. Ask the learners what they have observed. Who smelled the scent first? Who are the last ones to
smell the scent? How would you explain the phenomenon wherein learners in the same classroom
smelled the spray at different times?
1. Show an illustration of a plasma membrane to the learners.

2. Ask the learners to describe the plasma membrane.
3. Discuss the importance of the plasma membrane and how indispensable it is to the life of the cell.
4. Explain how plasma membranes are arranged in the presence of water.
5. Let the learners enumerate the structures found in a plasma membrane.
Teacher tip
Different responses to the question will be
drawn from students. Their responses will
depend on what aspect they are looking
into.
Acknowledge the responses of the learners.
Point out and explain that the two men are
both abnormal. Their growths are abnormal
such that one is too big in size and the
other one is too small. Both men have
defective membranes. Insufficient amount
of growth hormones pass through a
pygmys body while an excessive amount of
growth hormones is released in a giant.
6. Explain to the learners the structure of a phospholipid bilayer.
Phospholipids are the foundation of all known biological membranes. The lipid bilayer forms as a result of the interaction between the
nonpolar phospholipid tails, the polar phospholipid heads, and the surrounding water. The nonpolar tails face toward the water.
Transmembrane proteins float within the bilayer and serve as channels through which various molecules can pass.
7. Ask the learners to enumerate the different transport
mechanisms.
interior to accommodate the natural inward movement. Most

plants are hypertonic with respect to their immediate
environment. Osmotic pressure within the cell pushes the
cytoplasm against the cell wall and makes a plant cell rigid.
8. Differentiate between diffusion and osmosis.

9. Compare and contrast facilitated diffusion and active transport.
10. Present photos of plant and animal cells immersed in an

isotonic, hypotonic, and hypertonic solution.
To control the entrance and exit of particular molecules,

selective transport of materials is necessary. One simple process
is facilitated diffusion that utilizes protein transmembrane
channels that are specific to certain molecules. It is a passive
process driven by the concentration of molecules both inside
and the outside of the membrane. Certain molecules are
transported in and out of the cell, independent of concentration.
This process requires the expenditure of energy in the form of
ATP and is called active transport.
11. Describe solution and solute movement in and out of the cell
under hypertonic, hypotonic, and isotonic conditions.
12. Explain the effects of the different solutions to the cells. Ask
which among the three solutions is the best for plants? How
about for animals? Explain to the learners the water requirement
in plants.
Diffusion is the natural tendency for molecules to move

constantly. Their movement is random and is due to the energy
found in the individual molecules. Net diffusion occurs when the
materials on one side of the membrane have a different
concentration than the materials on the other side.
13. Differentiate among endocytosis, phagocytosis, pinocytosis,

receptor-mediated endocytosis, and exocytosis.
Large molecules enter the cell by generalized nonselective

process known as endocytosis. Phagocytosis is endocytosis of a
particulate material while endocytosis of liquid material is called
pinocytosis. Exocytosis is the reverse process. Receptormediated endocytosis is a complicated mechanism involving the
transport of materials via coated vesicles.
Osmosis is a special type of diffusion specifically associated with

the movement of water molecules. Many cells are isotonic to the
environment to avoid excessive inward and outward movement
of water. Other cells must constantly export water from their
48
PRACTICE (45 MINS)

Ask the learners to answer the following practice or guide
questions:
What is the difference between diffusion and facilitated

diffusion?
How do endocytosis and exocytosis allow movement of
materials in and out of the cell?
What solution is best for a plant cell? How about for an animal
cell?
Explain the orientation of the phospholipid molecules in the
presence of water.
Let the learners recognize the effect of a defective membrane in

normal body functioning. Ask them to write an essay about the
possible effects of a faulty plasma membrane aside from the
examples given earlier.
Ask the learners to individually submit a concept map about plasma
membrane and the different transport mechanisms.
Ask the learners to design and a model of a plasma membrane

using recyclable or indigenous materials.
Divide the learners into groups and assign different concentrations
of salt solution to be used in making salted eggs.
Ask the learners to answer the following questions:
Why does putting salt on meat preserve it from bacterial

spoilage?
Compare specific transport processes (i.e., diffusion, osmosis,
facilitated transport, active transport, endocytosis, and
exocytosis) in terms of the following:
concentration gradient
use of channel or carrier protein
use of energy
types or sizes of molecules transported
General Biology 1
240 MINS
Transport Mechanisms Pt.2
LESSON OUTLINE
Content Standard
Introduction Presentation of objectives and important terms;
Discussion on the structure of the plasma
The learners shall be able to construct a cell membrane model from indigenous
membrane; Brief discussion on the different
15
transport mechanisms
The learners shall be able to construct a cell membrane model from indigenous Motivation
The learners:
describe the structural components of the cell membrane

(STEM_BIO11/12Ig-h-11)
relate the structure and composition of the cell membrane to its function
explain transport mechanisms in cells (diffusion, osmosis, facilitated
transport, active transport) (STEM_BIO11/12Ig-h-13)
differentiate exocytosis and endocytosis (STEM_BIO11/12-Ig-h-14)

describe the plasma membrane

explain how plasma membranes are arranged in the presence of water
understand the structure of the phospholipid bilayer
describe and compare diffusion, osmosis, facilitated transport and active
transport
explain factors that affect the rate of diffusion across a cell membrane
predict the effects of hypertonic, isotonic, and hypotonic environments on
osmosis in animal cells
differentiate endocytosis (phagocytosis and pinocytosis) and exocytosis
Class activity to illustrate the process of diffusion;

Discussion of similarities between a giant and
pygmy; Demonstration of the principle behind the
process of making salted eggs
15
Instruction/
Delivery
Discussions, as a class and among groups, on the

structure and importance of the plasma
membrane and on the different transport
mechanisms
60
Practice
Answering of practice or guide questions
30
Enrichment
Essay writing or concept mapping; Class activity

on salted egg making
60
Evaluation
Construction of a plasma membrane model from

indigenous or recyclable materials; Concept
mapping on the different transport mechanisms;
Answering of questions for assessment
60
Materials
projector, laptop (if available), visual aids, school supplies, recycled or
indigenous materials
Resources
(1) Campbell, N.A. et. al. (2008). Biology 8th Edition Pearson International.
Pearson/Benjamin Cummings Publishing.
(2) Campbell, N. J. (2010). Biology 9th edition Pearson International Edition.
(3) Freeman, S. (2011). Biological Science. 4th edition. International Edition.
(4) Hickman, C. L. (2011). Integrated Principles of Zoology. 15th edition. McGraw
Hill Co., Inc.
50
Teacher tip
Prior to this lesson, instruct the learners to read up on the transport of materials across membranes. Ask
the learners to identify the different mechanisms on how materials are transported in and out of the
cell.
Introduce the topic by providing the learners with background information.
After the learners have enumerated the

different transport mechanisms, ask them
why they think there is a need to have
different kinds of processes that allow
materials to be transported in and out of
the cell.
In order for the cell to stay alive, it must meet the characteristics of life which include taking nutrients in
and eliminating wastes and other by-products of metabolism. Several mechanisms allow cells to carry
out these processes. All of the cells activities are, in one way or another, tied to the membrane that
separates its interior from the environment.
Ask the learners how they visualize a plasma membrane and what characteristics do they think are
essential for it to perform its function.
Learners will describe the plasma

membrane in different ways. Ask them how
they think the structures found within the
membrane help in performing its function
and what might happen in the absence of
the these structures
Teacher tip
Allow some time for the learners to smell

the spray until everyone has already smelled
the scent. Remember to instruct the
learners to raise their hand once they smell
the scent.
Before the start of the lesson on diffusion, conduct this simple class activity. Spray an air freshener in
one corner of the room and instruct the learners to raise their hands if they have smelled the scent of
the spray.
Ask the learners the following questions:
Who among the class were able to smell the air freshener first?
Who among the class were the last ones to smell the air freshener?
How would you explain the phenomenon wherein people in the same classroom smelled the
scent of the air freshener at different times?
Divide the learners into groups and ask them the question: What comes to your mind when you see
two men who are of the same age but one is 7.5 feet tall and the other is 3.5 feet tall?
Allow the learners to discuss the similarities and differences between the two among their groups.
Ask a representative from each group to present the results of their discussions to the whole class.
The learners might give varying responses

to the question depending on what aspect
they are looking into. Give hints by
providing the giant and pygmy as examples.
Acknowledge the learners responses and
point out that the two men are similar in the
sense that they are both abnormal. Growth
in both men is abnormal such that one is
too big in size while the other one is too
small.
Explain that both men have abnormal
growth. Both have defective membranes.
Insufficient amount of growth hormones
pass through a pygmys body while an
excessive amount of growth hormones is
released in a giant.
Teacher tip
Structure, function and importance of the plasma membrane

1. Present an illustration of the plasma membrane to the class
2. Ask the learners to describe the plasma membrane.
3. Discuss the importance of the plasma membrane and how indispensable it is to the life of the cell.
4. Explain how plasma membranes are arranged in the presence of water.
5. Let students enumerate structures found in a plasma membrane.
6. Make students understand the structure of a phospholipid bilayer.
Plasma membranesare made up of a phospholipid bilayer in an aqueous environment.
Phospholipids are the foundation of all known
biological membranes. The lipid bilayer forms as a
result of the interaction between the non-polar (hydrophobic or water-fearing)
phospholipid
tails, the polar (hydrophilic or water-loving) phospholipid heads, and the surrounding water.
The nonpolar tails face toward the water. Transmembrane proteins float within the bilayer and serve as
channels through which various
molecules can pass. They function as identification tags on cells
which enable the cell to determine if the other cells that it encounters
are like itself or not. It also
permits cells of the immune system to accept and reject foreign cells such as disease-causing bacteria.
Many membrane proteins function as enzymes that speed up reactions in cells. Others act like paste or
glue-forming cell junctions where
adjacent cells stick together. Membranes also contain cholesterol
which reduces the cells permeability to substances and make the bilayer stronger.
Transport Mechanisms
1. Ask the learners to enumerate the different transport mechanisms.
2. Differentiate between diffusion and osmosis.
52
You can ask the following questions before

starting the discussion:
Have you realized how crucial the task of a
plasma membrane is in maintaining the life
of a cell?
Have you thought about the ways on how
the materials needed by the cell and the
wastes it needs to dispose are able to move
in and out of the plasma membrane?
Molecules and substances move in several ways that fall within two categories: passive transport and active transport. In passive transport,
heat energy of the cellular environment provides all of the energy, hence, this is not energy-costly to the cell. Active transport, however, requires
the cell to do work, requiring the cell to expend its energy reserves.
Diffusion is a type of passive transport described as the natural tendency for molecules to move constantly. Their movement is random and is
due to the energy found in the individual molecules. Net diffusion occurs when the materials on one side of the membrane have a different
concentration than the materials on the other side. Osmosis is a special type of diffusion specifically associated with the movement of water
molecules.
A solution with a higher concentration of solutes is said to be hypertonic while a solution with a lower concentration of solutes is hypotonic.
Water crosses the membrane until the solute concentrations are equal on both sides. Solutions of equal solution concentration are said to be
isotonic. This only occurs when the solute concentration are the same on both sides of the membrane.
Compare and contrast facilitated diffusion and active transport. Then present photos of plant and animal cells immersed in an isotonic,
hypotonic, and hypertonic solution. In addition, describe a solution and solute movement into and out of the cell under hypertonic, hypotonic
and isotonic conditions.
Explain the effects of the different solutions to the cells. Ask which among the three solutions is the best for plants? For animals? Let them
understand water requirement in plants.
Many cells are isotonic to the environment in order to avoid excessive inward and outward movement of water. Other cells must constantly
export water from their interior to accommodate the natural inward movement. Most plants are hypertonic with respect to their immediate
environment. Osmotic pressure within the cell pushes the cytoplasm against the cell wall and makes a plant cell rigid.
Ask the learners the following questions:
How do cells behave in different solutions?

What do you notice about the effect of different solutions to animal and plant cells?
What solution is best for an animal cell? Does this hold true with plant cells?
When an animal cell such as red blood cell is immersed in an isotonic solution, the cell gains water at the same rate that it loses it. The cells
volume remains constant in this situation.
What will happen to the red blood cell when immersed in a hypotonic solution which has a lower solute concentration than the cell? The cell
gains water, swells, and may eventually burst due to excessive water intake. When placed in a hypertonic solution, an animal cell shrinks and
can die due to water loss.
Water requirement for plant cells is different due to their rigid cell walls. A plant cell placed in an isotonic solution is flaccid and a plant wilts in
this condition. In contrast with animal cells, a plant cell is turgid and healthy in a hypotonic solution. In a hypertonic solution, a plant cell loses
water, shrivels, and its plasma membrane detaches from the cell wall. This situation eventually causes death in plant cells.
Differentiate diffusion from facilitated diffusion.
To control the entrance and exit of particular molecules, selective transport of materials is necessary. One simple process is facilitated diffusion
that utilizes protein transmembrane channels that are specific to certain molecules. It is a passive process driven by the concentration of
molecules on the inside and the outside of the membrane. Certain molecules are transported in and out of the cell, independent of
concentration. This process requires the expenditure of energy in the form of ATP and is called active transport.
Differentiate endocytosis, phagocytosis, pinocytosis, receptor-mediated endocytosis, and exocytosis.

Large molecules enter the cell by generalized non-selective process known as endocytosis. Phagocytosis is endocytosis of a particulate
material while pinocytosis is endocytosis of liquid material. In this process, the plasma membrane engulfs the particle or fluid droplet and
pinches off a membranous sac or vesicle with a particular fluid inside into the cytoplasm.
Exocytosis is the reverse process where a membrane-bound vesicle filled with bulky materials moves to the plasma membrane and fuses with
it. In this process, the vehicles contents are released out of the cell.
Receptor-mediated endocytosis is a complicated mechanism involving the transport of materials through coated vesicles. Cells take up
molecules more efficiently in this process due to the receptor proteins on their surfaces. Each receptor protein bears a binding site for a
particular molecule. If the right molecule contacts a receptor protein, it attaches to the binding site, forming a pocket and eventually pinching
off into the cytoplasm.
PRACTICE (30 MINS)
Ask the learners to answer the following questions:
Explain the orientation of the phospholipid molecules in the presence of water.

Enumerate the structures found in a plasma membrane and give the function of each.
54
How do diffusion and facilitated diffusion differ?

How do endocytosis and exocytosis allow movement of materials in and out of the cell?
What solution is best for a plant cell? How about for an animal cell?
Give two ways by which one could determine whether active transport is going on.
Compare and contrast the effects of hypertonic and hypotonic solutions on plant and animal cells.
What role do vacuoles play in endocytosis and exocytosis?

Essay writing and concept mapping
1. Ask the learners to write an essay about the possible effects of a faulty plasma membrane aside
from the examples given in the lesson. Let the learners recognize the effects of a defective membrane
to normal bodily functions.
2. Ask the learners to individually submit a concept map about the plasma membrane. You can provide
them with sample words for their concept map:
plasma membrane
semipermeable
phospholipid bilayer
hydrophilic heads
hydrophobic tails
cholesterol
membrane proteins
Teacher tip
For the concept mapping, you can provide
the learners with key words or allow them to
come up with their own key words for their
concept map.
Creating own saturated salt solution for salted egg-making

1. Divide the class into groups and assign different concentrations of salt solutions to be used in
making salted eggs.
2. Instruct the learners to make their own salt solutions and take note of the concentration that they opt
to use.
Teacher tip
Diffusion and osmosis are two processes
involved in making salted eggs. The salt
solution should be supersaturated in order
to produce good and delicious salted eggs.

Building of plasma membrane model
1. Divide the class into groups.
2. Ask the groups to design and build a model of a plasma membrane using recyclable or indigenous
materials.
Concept mapping
Ask the learners to individually submit a concept map about the different transport mechanisms. You
can provide them with sample words for their concept map or allow them to come up with their own:
plasma membrane
phagocytosis
transport mechanisms
pinocytosis
passive transport
receptor-mediated
endocytosis
active transport
diffusion
hypotonic
facilitated diffusion
hypertonic
endocytosis
isotonic
exocytosis
Assessment questions:
Instruct the learners to answer the following questions to assess their knowledge and understanding of
the lesson:
Why does putting salt on meat preserve it from spoilage by bacteria?

Compare specific transport processes (i.e., diffusion, osmosis, facilitated transport, active transport,
endocytosis, and exocytosis) in terms of the following:
concentration gradient
use of channel or carrier protein
use of energy
types or sizes of molecules transported
56
Teacher tip
You can provide the learners with key words
or allow them to come up with their own
key words for their concept map.
General Biology 1
Carbohydrates and Lipids:

Structures and Functions
of Biological Molecules
Content Standard
The learners demonstrate an understanding of the structures and functions of
carbohydrates and lipids and their roles in specific metabolic processes.
The learners shall be able to explain the role and significance of carbohydrates
and lipids in biological systems.
The learners:
categorize the biological molecule as a carbohydrate or lipid according to

their structure and function (STEM_BIO11/12-Ii-j-15)
explain the role of each biological molecule in specific metabolic processes
(STEM_BIO11/12-Ii-j-16)
detect the presence of carbohydrates and lipids in food products using
simple tests

120 MINS
LESSON OUTLINE
Introduction Presentation of learning objectives and
important terms; Discussion on dehydration
reactions and hydrolysis
Motivation
10
Relating the lessons to real-life situations;

Discussion on food as sources of energy and
building blocks
10
Instruction/ Discussion, as a class and among groups, on

Delivery/
the structure and importance of
Practice
carbohydrates and lipids.
60
Enrichment Laboratory activity on testing the
20
Evaluation
20
presence of carbohydrates and lipids on

common food products
Group activity on making molecular
models of carbohydrates and lipids
Materials
projector, laptop (if available), sample food labels, common

food or drink products (e.g. flour, cornstarch, cooking oil,
present simple molecular models of carbohydrates and lipids and relate the
food or drink brought by the learners
structure to the roles that these molecules play in biological systems
perform tests for the presence of starch and reducing sugars and lipids on
Resources
common food products
(1) Reece, J.U. (2011). Campbell Biology, 9th ed. San Francisco, CA:
Pearson Benjamin Cummings
Teacher tip
Communicate learning objectives and important terms
Prominently display the learning objectives

and important terms on one side of the
classroom and frequently refer to them
during the discussion. You may place a
check-mark beside a term in the wordlist
after defining it so that the learners have an
idea of their progress.
Introduce the following learning objectives using any of the suggested protocols (i.e., verbatim, own
words, or read-aloud)
I can distinguish a carbohydrate from a lipid given its chemical structure and function.
I can explain the roles played by carbohydrates and lipids in biological systems.
I can detect the presence of carbohydrates and lipids in food products using simple chemical tests.
Introduce the list of important terms that learners will encounter in this lesson:
macromolecule
cellulose
polymer
chitin
monomer
lipids
dehydration reaction
fat
hydrolysis
fatty acid
carbohydrates
triacylglycerol
monosaccharides
saturated fatty acid
disaccharides
unsaturated fatty acid
glycosidic linkage
trans fat
polysaccharide
phospholipids
starch
steroids
glycogen
cholesterol
1. Divide the class into groups of three.

2. Distribute sample food or nutrition labels to each group and ask them if they know how to interpret
the information written on the food labels.
58
Each learner can also illustrate or define the

term on a sheet of paper which can be
tacked beside the list of words.
Another way of incorporating lists of
important terms is to have the words placed
in a blank bingo card grid.
Learners can write a short definition or
description of the term under the entry in
the bingo card to block out a square. This
may serve as the learners reference guide
or method of formative assessment.
You may ask the following questions to facilitate the

discussion and call on several groups to present in front of
the class:
How many servings are in this container?
Would you agree that this is the reasonable amount of
food you would consume per serving? How many total
food calories (C) are in this container?
How much fat is present in one serving? What kind of fat?
What is the importance of consuming fats in our diet?
How much carbohydrates are present in one serving?
What kind of carbohydrates? What is the importance of
consuming carbohydrates in our diet?
Decide on whether this food sample can be eaten often
or sparingly and justify.
3.Recall that human beings, like all animals, are

heterotrophs that need to take in energy and organic
molecules (carbohydrates, fats, and proteins) from plant
and animal matter.
4.Explain to the learners that this lesson will describe the
structure of carbohydrates and lipids and explain the role that these biomolecules play in important
biological processes.
Teacher tip
For the food labels, local products that are
familiar to the learners will make the best
samples. Make sure that the labels have
carbohydrates, fats, and fibers in them. If
there are no food labels available, you may
do an image search and print some sample
food labels from the internet.
Division into small groups of two or three
may facilitate sharing. Only call on two or
three groups to present if there is limited
time.
Expect the responses to vary depending on
how realistic the serving sizes are. You can
also discuss about how advertisers can
influence how people perceive food.
Take note that a food calorie is the same as
1 kcal or 1000 calories. A young adult would
often need to take 1800-2500C per day
depending on their size and level of activity.
Responses may include saturated,

unsaturated, and trans fats. Explain to the
learners that these fats will be discussed in
more detail during the lesson. Regarding its
importance, expect responses ranging from
energy source, insulation, for flavor, for aid
in cooking, for heart health, skin health, etc.
Possible responses include sugar, fibers, etc.
Regarding its importance, responses may
include energy source, for aid in regular
bowel movement, for provision of building
blocks for biosynthesis, etc.

Present a diagram similar to the one below.
Table 1: Abundant elements in the human body (Source: http://www.personal.psu.edu/staff/m/b/

mbt102/bisci4online/chemistry/elementsorgnsm.jpg)
Point out that the bulk (i.e., more than 90%) of the human body weight is provided by only three
elements: oxygen, carbon, and hydrogen. We get these elements primarily from the food we eat, from
the water we drink, and from the air we inhale around us.
Explain to the learners that biogeochemical cycles such as the carbon-oxygen cycle and the water cycle
play important roles in ensuring that we have access to these important elements. All forms of life, not
only that of humans, are made up of four kinds of important large molecules: carbohydrates, lipids,
60
proteins, and nucleic acids. All of these have carbon atoms as their backbones since carbon is capable of forming up to four chemical bonds
with atoms of other elements.
Facilitate the lecture on carbohydrates and lipids.
What do humans get from food?
Heterotrophs, such as human beings, obtain energy and raw materials from food. These are important for cell growth, cell division, metabolism,
repair, and maintenance of the body. Nutrients can be classified as either organic nutrients (i.e., those that contain carbon such as
carbohydrates, fats, proteins, vitamins, and nucleic acids) or inorganic nutrients (i.e., those that do not contain carbon such as water and mineral
salts).
What are carbohydrates?
Carbohydrates are organic compounds made up of carbon, hydrogen, and oxygen. These compounds have a general formula of
CnH2mOm. This means that the hydrogen and oxygen atoms are present in a ratio of 2:1. For example, glucose has a formula of
C6H12O6 and sucrose has a formula of C12H22O11.
Carbohydrates are usually good sources of raw materials for other organic molecules and energy. One gram of carbohydrates provides
four food calories or 16 kJ of energy. In the human diet, carbohydrates mainly come from plants although they are found in all
organisms.
How are carbohydrates formed?
Carbohydrates are examples of macromolecules. These are chainlike molecules called polymers (mere means part) made from repeating units
like monomers. Polymers can be formed from covalently-bonded monomers much like a single structure can be made out of repeated building
blocks linked to each other.
These monomers, called monosaccharides, form covalent bonds when one monomer loses a hydroxyl group and the other loses a hydrogen
atom in dehydration or condensation reactions, forming disaccharides. This reaction requires energy to occur. The bond formed is called a
glycosidic linkage.
Teacher tip
Figure 2: Dehydration synthesis of disaccharides from monosaccharide components (Source: https://

bealbio.wikispaces.com/file/view/disaccharides.JPG/364413582/disaccharides.JPG)
Longer polysaccharide chains are formed by monomer addition through succeeding dehydration
reactions. These reactions can occur in the human liver as carbohydrates are stored as polysaccharides
called glycogen or in ground tissues of plants where these are stored as starch.
Polysaccharides are broken down into simpler components through the use of water to break covalent
bonds and release energy. The process, known as hydrolysis (hydro means water and lysis means split),
is the opposite of dehydration reactions and often occurs in the digestive tract during chemical and
mechanical digestion. Here, enzymes break bonds within polysaccharides. With the aid of water, one
H group attaches to a monosaccharide while another OH group attaches to the other.
Comprehension question: How many molecules of water are needed to completely hydrolyze a
polysaccharide that is one thousand monosaccharides long?
62
Use ball and stick models or plastic blocks

to demonstrate how dehydration and
hydrolysis reactions occur. Simple reusable
ones may be constructed from toothpicks or
clay or similar materials.
If a projector is available, you may also use

animations like the ones found at <http://
www.cengage.com/biology/
discipline_content/animations/
reaction_types.swfto> to help in
visualization.
Correct response: 999 water molecules
During the discussion, invite the learners to
find different kinds of carbohydrates in their
food labels.
How are carbohydrates classified?

Carbohydrates can be classified into three main categories, according to increasing complexity:
monosaccharides (monos means single and sacchar means sugar)
disaccharides (di means two)

polysaccharides (poly means many)
Some notes on their structures and functions are found in the following table:
Classification
Monosaccharide
Functions
major cellular
nutrient
often
incorporated
into more
complex
carbohydrates
Structure
contains a carbonyl group

(C=O) and may be
classified as an aldose or
ketose depending on the
position
may have three to seven
carbons in the skeleton
may be arranged in a
linear form when solid
and is converted into a
ring form in aqueous

solution ( form when H is
on top of plane of ring
and form when -OH is
on top of plane of ring)
Examples
Ribosea 5C aldose that

forms part of the
backbone of nucleic
acids
Glucosea 6C aldose
that is the product of
photosynthesis and the
substrate for respiration
that provides energy for
cellular activities
Fructosea 6C ketose
that is found in many
plants and is often
bonded to glucose
Classification
Disaccharide
Functions
energy
source
sweetener
and dietary
component
Structure
forms when a
glycosidic linkage
forms between
two
monosaccharides
Examples
Polysaccharide
storage
material for
important
monosaccharides
structural
material for the
cell or the entire
organism
forms when
hundreds to thousands
of monosaccharides are
joined by glycosidic
linkages
Maltose (glucose + glucose)malt sugar often

found in sprouting grains, malt-based energy
drinks, or beer
Lactose (glucose + galactose)milk sugar that
is a source of energy for infants; an enzyme
called lactase is required to digest this. Many
adult Filipinos have low levels of this enzyme
leading to a condition called lactose
intolerance.
Sucrose (glucose + fructose)found in table
sugar processed from sugar cane, sweet fruits,
and storage roots like carrots
Storage polysaccharides are large molecules
retained in the cell and are insoluble in water
(formed from 1,4 linkage monomers; with a helical
structure)
o Starchamylase is unbranched starch forming a
helical structure while amylopectin is branched
starch, these are present in plant parts like potato
tubers, corn, and rice and serve as major sources of
energy.
o
Glycogenfound in animals and fungi; often
found in liver cells and muscle cells
Structural polysaccharides (formed from 1,4
linkage of monomers; strands associate to form a
sheet-like structure)
o
Cellulosetough sheet-like structures that
make up plant and algal cell walls that may be
processed to form paper and paper-based products;
humans lack the enzymes to digest 1,4 linkages so
is passed out of the digestive tract and aids in
regular bowel movement
o
Chitinused for structural support in the walls
of fungi and in external skeletons of arthropods
o
Peptidoglycanused for structural support in
bacterial cell walls
Teacher tip
Examples of alpha helices and beta
sheets may be created using wire for
the backbone and yarn for the Hbonds; invite learners to speculate on
why alpha helix structures are
associated with storage
polysaccharides and beta sheets with
structural polysaccharides.
Teacher tip
Invite learners to compare the rigidity
or structural integrity of plant matter
or paper, a shrimps shell, and a
mushroom. Explain that all these
structures are formed from sheets.
What are lipids?
Teacher tip
Lipids are a class of large biomolecules that are not formed through polymerization. They have diverse
structures but are all non-polar and mix poorly, if at all, with water. They may have some oxygen atoms
in their structure but the bulk is composed of abundant nonpolar C-H bonds. They function for energy
storage, providing nine food calories or 37 kJ of energy per gram. They also function for the cushioning
of vital organs and for insulation. Furthermore, they play important roles in plasma membrane structure
and serve as precursors for important reproductive hormones.
Fats or triacylglycerol formation may be

explained better using a diagram such as
the one below or through models patterned
after a similar diagram. You may ask the
learners to explain, in their own words, what
they think is happening and compare the
formation of carbohydrates with that of
lipids.
How are lipids classified?

Lipids can be divided into three main classes according to differences in structure and function. Some
notes on their structures and functions are found in the following table:
Classification
Functions
Fats
(triacylglycerols
or triglycerides)
energy
storage
cushioning of
vital organs
(adipose
tissue)
insulation
Structure
formed from dehydration

reactions between glycerol (an
alcohol with three Cs, each with
an OH group) forming three
ester linkages with three fatty
acids (16-18 Cs, with the last C as
part of a COOH group) and
producing three molecules of
water
component fatty acids (FA) may
be either saturated or
unsaturated
o
Saturated FA (e.g., palmitic
acid) have the maximum number
of hydrogen atoms bonded to
each carbon (saturated with
hydrogen); there are no double
bonds between carbon atoms
o
Unsaturated FA (e.g., oleic
acid) have at least one double
bond, H atoms are arranged
around the double bond in a cis
configuration (same side)
resulting in a bend in the
structure
Teacher tip
Demonstrate the effects of the straight

chains of saturated FAs on packing by piling
together flat structures like books or
blackboard erasers and ask learners to
Examples
compare this with the stacking or packing of
Saturated fatanimal products such as irregularly shaped objects like partiallybutter and lard have a lot of saturated fatty acids. folded sheets of cardboard.
The linear structure allows for the close packing of
the fat molecules for ming solids at room
temperature, diets high in these fats may increase
the risk of developing atherosclerosis, a condition in
which fatty deposits develop within the walls of
blood vessels, increasing the incidence of
cardiovascular disease
Unsaturated fatplant and fish oils have

unsaturated fatty acids. The bent structure prevents
close packing and results in oils or fats that are liquid
at room temperature. Homemade peanut butter has
oils that separate out of solution for this reason.
Industries have developed a process called
hydrogenation that converts unsaturated fats into
saturated fats to improve texture spreadability.
Trans fatmay be produced artificially

through the process of hydrogenation described
above. The cis double bonds are converted to trans
double bonds (H atoms on opposite sides) resulting
in fats that behave like saturated fats. Studies show
that trans fat are even more dangerous to health
than saturated fats to the extent that they have been
banned from restaurants in some countries.
During discussion, invite the learners to find

different kinds of fats in their food labels
and decide on whether a particular food is
healthier than another based on its fat
content.
Misconception
Clarify the misconception that consuming
fats is entirely dangerous for health. Fats are
an essential part of a healthy diet when
consumed in moderation.
Classification
Phospholipids
Functions
major component
of cell membranes
Structure
formed from dehydration
reactions between glycerol
(an alcohol with three Cs,
each with a OH group),
forming two ester linkages
with two fatty acids (16-18
Cs, with the last C as part of
a COOH group) and a last
linkage with a phosphate
group
Examples
Phospholipids self-assemble
into bilayers when
surrounded by water and
form the characteristic
structure of plasma
membranes
Steroid structure may be explained better

using a diagram such as the one below or
through models patterned after a similar
diagram. You may ask the learners to
describe the diagram in their own words
and compare the structure of cholesterol
with that of other lipids.
Steroids and
sterols
regulate
fluidity of cell
membranes
base of sex
hormones
emulsification of
fats during
digestion
functional group
attached to the rings vary (if
OH is attached to the 4th C, then
it is called a cholesterol)
66
Phospholipid structure may be explained

better using a diagram such as the one
below or through models patterned after a
similar diagram. You may ask the learners to
describe the diagram in their own words
and compare the structure of fats with that
of phospholipids.
Teacher tip
phosphate group is
hydrophilic and is called
the head of the molecule
fatty acids are hydrophobic

and form the tails of the
molecule
characterized by a Cskeleton with four fused rings
Teacher tip
Cholesterol found in
cell membranes regulates
the rigidity of the cell
membrane and are the base
material for the production
of sex hormones like
estradiol and progesterone
Divide the class into groups. Instruct the learners to prepare the following materials that are needed for
the laboratory activity:
eight glass droppers, medicine droppers, or caps
ethanol solution
12 test tubes
glucose solution
test tube holders or tongs
flour or cornstarch
beaker
cooking oil
alcohol lamp
sample of studentbrought food or drink
Benedicts solution
iodine solution
mortar and pestle
Explain the following processes to the learners.
Benedicts solution, a blue solution with CuSO4(aq), can detect the presence of reducing sugars (i.e.,
any sugar with a free aldehyde or ketone group such as all monosaccharides and the disaccharides
lactose and maltose). When boiled, these sugars reduce Cu2+ in Benedicts solution to produce a brickred precipitate of Cu2O(s).
Iodine test can be used to detect the presence of starch.
Teacher tip
This activity may be done as a class if time
does not permit for the activity to be done
in separate groups. If Benedicts solution is
not available, you may only perform the last
two tests.
In the absence of laboratory grade
chemicals, you may improvise with storebought chemicals like iodine and 70% ethyl
alcohol for medical use. Make sure to test
the procedure before performing the
activity in the class.
Emulsion test can be used to identify fats.
Learners should perform all three tests on the following samples:

glucose solution (available in the baking section of grocery
stores)
cooking oil
food or drink sample

that the learners
flour or cornstarch solution
brought.
For solid samples, instruct the learners to mash a small portion of the sample in some water using the
mortar and pestle and then test the resulting solution. Ask the learners to prepare a table with
appropriate headings in which to record their results.
In discussing the results, ask the learners to conclude whether carbohydrates or lipids are present in
their samples. They may compare this with the list of ingredients for their food or drink sample. They
can also list possible sources of errors.
Divide the class into small groups. Provide the groups with different structures of lipids or
carbohydrates and ask them to create models using common or recyclable materials.
Ask the learners to explain or write a short description of their models. In grading the models, check to
see if the learners were able to create an accurate model of the assigned lipid or carbohydrate.
Ask the learners, still in their small groups, to create a short flowchart that will allow them to distinguish
between the different kinds of carbohydrates and lipids based on their structures. They may use this
flowchart in answering the comprehension questions that follow.
Provide different molecular structures of the following and ask the learners to identify whether these
are:
68
Teacher tip
Prior to this lesson, instruct the learners to
bring recyclable materials that they can use
for this activity.
monosaccharides
disaccharides
unsaturated fats
storage polysaccharides
phospholipids
structural polysaccharides
steroids.
saturated fats
You may also ask the learners to give one of the associated functions or characteristics of the given
carbohydrate or lipid.
Teacher tip
The various carbohydrate structures were
obtained from the following electronic
resources:
commons.wikimedia.org
http://www.nature.com/pj/journal/v43/
n12/images/pj201196f3.jpg
http://chemwiki.ucdavis.edu/@api/deki/
files/522/260px-Cellulose_strand.jpg?
size=bestfit&width=352&height=310&r
evision=1
Images for the various lipid structures were

obtained from the following electronic
resources:
https://upload.wikimedia.org,
http://www.mikeblaber.org/oldwine/
BCH4053/Lecture13/triglyceride.jpg,
https://my.bpcc.edu/content/blgy225/
Biomolecules/phospholipid.gif
Biographical Notes
FLORENCIA G. CLAVERIA, Ph.D.
Team Leader
DAWN T. CRISOLOGO
Team Leader
Dr. Florencia G. Claveria is the current Chair of

the CHED Technical Panel for Biology and Molecular
Biology. She is also member of the Commissions
Technical Panel for Math and Science. She is currently
Vice Chancellor for Academics, Research, and
Operations at the De La Salle Araneta University.
She is a full professor at the De La Salle
University-Manila where she served as Dean of the
College of Science for 6 academic years. Dr Claveria
finished her doctorate in Biological Sciences at the
University of Cincinnati, through a Fulbright-Hays grant.
She completed her masters in Zoology at the Ghen
State University, through a grant from the Government
of Belgium. She earned her bachelors degree in
Biology at St. Louis University. Her written scholarly
works include contributions to academic publications
such as the Philippine Textbook of Medical
Parasitology, Journal of Protozoology Research, and
The Journal of Veterinary Medical Science.
Ms. Dawn Crisologo is a Special Science Teacher at the Philippine

Science High School-Main Campus in Diliman, Quezon City and specializes
in advanced topics in Ecology, Evolution and Biodiversity, Anatomy,
Physiology, and Methods in Science and Technology Research. She is a
member of the Asian Association of Biology Educators, Wildlife
Conservation Society of the Philippines, and Biology Teachers Association
of the Philippines. Her works are included in The Philippine BIOTA Journal
and three editions of the Science Blast textbook. Ms. Crisologo is currently
finishing her masters in Environmental Science at the University of the
Philippines Diliman. She completed her bachelors degree in Biology at the
same university.
CHUCKIE FER CALSADO

Writer
Mr. Chuckie Fer Calsado is Special Science Teacher IV at the
Philippine Science High School Main Campus where he has been teaching
for 8 years. He is a member of biological organisations like the Biology
Teachers Association of the Philippines, the Asian Association for Biology
Education, and Concerned Artists of the Philippines among many others.
He has published academic papers such as Implication of Students
Cognitive Style, Personal Demographics, Values and Decision Making in
Environmental Education and the Role of Education in the Prevention of
Child Trafficking in Nepal. Mr. Calsado finished his Masters in Bioethics at
the Monash University and his bachelors degree in Biology at the
University of the Philippines DIliman.
184
AILEEN C. DELA CRUZ

Writer
JANET S. ESTACION, Ph.D.

Writer
Ms. Aileen Dela Cruz has been serving as the Science

Research Analyst at the Philippine Science High School - Main
Campus since 2004. Her academic interests range from
microbiology, food safety and nutrition, and laboratory safety and
she has been involved in trainings and conferences on the same
fields of study. Her published scholarly works include series of
textbooks on 21st Century Learning. Ms. Dela Cruz earned her
bachelors degree in Biology at the University of the Philippines
Baguio.
Dr. Janet Estacion is current Officer-in-Charge at the

Institute of Marine and Environmental Science in Silliman Unive
DOREEN D. DOMINGO, PH.D.

Writer
Dr. Doreen D. Domingo is a Professor at the Mariano
Marcos State University where she teaches both in the graduate
and undergraduate levels. She is currently the Chief of Alumni
Relations for the university. Dr. Domingo finished her doctorate in
Biology (magna cum laude) at St. Louis University through a
research grant from CHED and the Microbial Forensics and
Biodefense Laboratory, Indiana University. She completed her
Doctor of Education on Educational Management, her masters
degree in Education major in Biology, and her bachelors degree
in Biology at the Mariano Marcos State University. Dr. Domingos
scholarly works were published on the International Referred
Journal and the National Referred Journal.
rsity where she has been teaching for 30 years now. She headed
researches on marine conservation and the recovery of reefs. Her
scholarly works appeared on different publications such as the
Philippine Science Letters and the Silliman Journal. Dr. Estacion
earned her doctorate degree in Zoology at the James Cook
University of North Queensland. She completed her masters
degree in Marine Biology at the University of the Philippines
Diliman and her bachelors degree in Biology at the Silliman
University.
MARY JANE C. FLORES, Ph.D.

Writer
Dr. Mary Jane C. Flores is Assistant Professor 3 at the
College of Science in the De La Salle University where she has
been teaching for 20 years now. Her published works include
researches on parasitology, climatology, and community
nutrition. Dr Flores has conducted and attended seminars on
Biology in the country and abroad, including the Training on
Biological Control at the US Department of AgricultureAgricultural Research Service and Congress meetings on
Parasitology. She is a two-time recipient of the Don Ramon J.
Araneta Chair in Ecology among other citations. Dr. Flores
earned her Doctorate, Masters, and Bachelors degrees in
Biology at the De La Salle University.
JOHN DONNIE RAMOS, Ph.D.

Technical Editor
JUSTIN RAY M. GUCE

Writer
Mr. Justin Ray Guce is a Special Science Teacher I at the

Philippine Science High School Main Campus in DIliman, Quezon
City where he teaches for 9 years. He has served as a Trainer of
student representatives for Science Olympiad competitions and
has delivered presentations in a number of Biology workshops
and conventions. Mr Guce is a member of the Wildlife
Conservation Society of the Philippines and the Biology Teachers
Association of the Philippines. Mr Guce is currently finishing his
masters in Biology Education at the University of the Philippines
Diliman where he also graduated his bachelors degree in
Biology.
NOLASCO H. SABLAN
Writer
Mr. Nolasco Sablan is Teacher III at the Parada National
High School and is a DepEd teacher for 11 years now. He has
worked as resource speaker, trainer, and writer for different
institutions in the education sector, including the Ateneo de
Manila University, Metrobank Foundation Inc., and the
Department of Education. Mr. Nolasco Sablan earned his
masters degree in Biology Education at the Ateneo de Manila
University and completed his bachelors degree in Education
major in General Science at the Philippine Normal University.
186
Dr. John Donnie Ramos is a Member of CHEDs Technical

Panel for Biology and Microbiology and Board Member of the
Philippine Society for Biochemistry and Molecular Biology. He is
currently the Dean of the College of Science at the University of
Santo Tomas where he teaches molecular biology, immunology
and genetics, and allergology. Dr. Ramos completed his
doctorate in Molecular Biology at the National University of
Singapore. He finished his masters degree in Biological Sciences
at the University of Santo Tomas and his bachelors degree in
Biology at the Philippine Normal University. Dr. Ramos is
recipient of the NAST-TWAS Prize for Young Scientist in the
Philippines in 2010, and Outstanding Young Scientist by the
National Academy of Science and Technology in 2005.
JOY R. JIMENA
Copyreader
Ms. Joy Jimena is currently Planning Officer II at the
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Exhibit. Ms. Go graduated her bachelors degree in Fine Arts
Major in Visual Communication at the University of the Philippine
Diliman.

Biology 1 SHS

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Biology 1 SHS

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The Commission on Higher Education

in collaboration with the Philippine Normal University

INITIAL RELEASE: 13 JUNE 2016

Teaching Guide for Senior High School

SPECIALIZED SUBJECT | ACADEMIC - STEM

Published by the Commission on Higher Education, 2016

University President: Ester B. Ogena, Ph.D.

Technical Editor: John Donnie A. Ramos, Ph.D.

Senior High School Support Team

CHED K to 12 Transition Program Management Unit

This Teaching Guide by the

DepEd General Biology 1 Curriculum Guide . . . . . . . . . . . . .

Chapter 3: Energy Transformation

Lesson 11: Photosynthesis and Cellular Respiration . . . . . . . . . . .

Lesson 1: The Cell: Endomembrane System, Mitochondria,

Chloroplasts, Cytoskeleton, and Extracellular Components . . .

Lesson 12: Forms of Energy, Laws of Energy Transformation

Lesson 2: Mitochondria and Chloroplasts . . . . . . . . . . . . . . . . .

Lesson 13: Energy Transformation Part 1 . . . . . . . . . . . . . . . . . . . . 111

Lesson 3: Structure and Functions of Animal Tissues and Cell

Lesson 15: Energy Transformation Part 3 . . . . . . . . . . . . . . . . . . . . 128

Lesson 4: Cell Cycle and Cell Division . . . . . . . . . . . . . . . . . . . . 36

Lesson 16: Cellular Respiration Part 1 . . . . . . . . . . . . . . . . . . . . . .

Lesson 5: Transport Mechanisms Part 1 . . . . . . . . . . . . . . . . . . . 46

Lesson 17: Cellular Respiration Part 2 . . . . . . . . . . . . . . . . . . . . . .

Lesson 6: Transport Mechanisms Part 2 . . . . . . . . . . . . . . . . . . . 50

Lesson 18: Cellular Respiration Part 3 . . . . . . . . . . . . . . . . . . . . . .

Chapter 2: Biological Molecules

Lesson 19: ATP in Cellular Metabolism and Photosynthesis . . . . .

Lesson 7: Carbohydrates and Lipids . . . . . . . . . . . . . . . . . . . . .

Lesson 8: Amino Acids and Proteins Part 1 . . . . . . . . . . . . . . . .

Lesson 9: Amino Acids and Proteins Part 2 . . . . . . . . . . . . . . . .

Lesson 10: Biological Molecules: Enzymes . . . . . . . . . . . . . . . .

Biographical Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 184

SHS for SHS

Why is this important?

How will I deeply understand this?

What can I do with this?

Through this Teaching Guide,

Given that developing mastery

When teachers empower

Biology I is a Science, Technology, Engineering and Mathematics (STEM) Specialized Subject

On DepEd Functional Skills and CHED College Readiness Standards

On the other hand, the Commission declared the College

College Readiness Standards Foundational Skills

DepEd Functional Skills

Solid grounding on Philippine experience and culture;

Global awareness, scientific and economic literacy, curiosity,

Global awareness, media literacy, technological literacy,

Global awareness, multicultural literacy, collaboration and

Media literacy, multicultural literacy, global awareness,

The Cell: Endomembrane System, Mitochondria,

Chloroplasts, Cytoskeleton, and Extracellular Components

prokaryotic and eukaryotic cells; submission

Brief class activity on prokaryotic and

Instruction/ Lecture. Board work on cell parts, structure,

Hydrilla cells under a microscope. Class

Enrichment Class discussion on cell size and relationship

Assessment of learners knowledge;

microscope (slide, cover slip), hand-held

Resources (continued at the end of Teaching Guide)

(2) (n.d.). Retrieved from <http://biology.tutorvista.com/animal-and-plant(3) (n.d.). Retrieved from <http://sciencenetlinks.com/lessons/cells-2-thecell-as-a-system/>

The review on the differences between

Smooth Endoplasmic Reticulum