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ABSTRACT
Efficacy of eight plant extracts viz., tulsi (Ocimum sanctum), neem (Azadirachtaindica), karanja
(Pongamiaglabra), papaya (Carica papaya), aloe (Aloe vera), turmeric (Curcuma longa), lantana (Lantana camera),
thuja (Thujaoccidentalis); three organics viz., Wood ash, rice gruel, vermicompost and one bioagent viz.,
Trichodermaviridewere preliminarily evaluated @ 33 per cent against Bipolarisoryzae in vitro by poisoned food
technique and bioagent by dual culture method. All the extracts, organics and bioagent were found to be significantly
inhibited the radial growth of the pathogen. Out of these, four promising extracts were further tested at different
concentrations viz., 0.10, 0.25 and 0.50 per cent in vitro. Tulsi (Ocimum sanctum) was significantly superior in
papaya (Carica papaya). Among the concentrations, 0.5 per cent was significantly superior than the other two
concentrations. Based on the results of in vitro test, the effective plant extracts were further tested at different
concentrations viz., 10, 20 and 30 per cent in pot condition. Results under pot condition showed that seed treatment
with all the four extracts could significantly reduce Percent disease incidence of brown spot of paddy as compared to
control. However, tulsi (Ocimum sanctum) @ 30 per cent showed maximum reduction of disease as compared to others,
but efficacy was lower than carbendazim @ 0.2 per cent.
Original Article
inhibiting the radial growth of Bipolarisoryzae followed by neem (Azadirachtaindica), karanja (Pongamiaglabra) and
Received: May 16, 2016; Accepted: May 31, 2016; Published: Jun 10, 2016; Paper Id.: IJASRJUN2016059
INTRODUCTION
Rice (Oryza sativa) is one of the most important staple food in the world after wheat, especially in Asia
and West Indies. In India, rice occupies an area of 43.8 m ha with an average production of 96.40 million tonnes
with productivity of 2.17 t/ha (Anon., 2012). Every year the demand for rice is growing up gradually due to
population growth and it is estimated that in 2025 AD, the requirement would be 140 million tonnes
(Gujja and Thiyagarajan, 2013). Therefore, future rice production targets must be achieved through only yield
improvement. Rice suffers from a number of fungal diseases.
Among these diseases, Brown spot of rice caused by Bipolarisoryzae (Breda. de Haan) Shoemaker is
important both economically and historically. Brown spot is widely reported across India (Reddy et al., 2010) and
more generally in the South and South-East Asian countries (Savary et al., 2000).
It causes yield losses that, on average, are in the range of 10 per cent of the attainable yield wherever it
occurs (Savary et al., 2006) in the lowlands of tropical and subtropical Asia. Therefore, brown spot is by far one
the strongest yield reducers amongst rice diseases today. Further, there is indication that brown spot is becoming
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more frequent and severe as drought is becoming more frequent (Savaryet al., 2005), perhaps due to increased variability
in rainfall.
The range of reported yield losses to brown spot, often expressed in relative terms, was variable from 4 to 29 per
cent (Bedi and Gill, 1960), about 12 per cent (Aluko, 1975), from 8 to 23 per cent (Fomba and Singh, 1990) from 26 to 52
per cent (Chakrabarti, 2001). The latter figures represented a broader and higher range because it accounts for losses
caused by grain infection.
A large number of chemicals have been developed for the control of brown spot disease. But, due to overgrowing
hazardous side effects of these chemicals, more and more emphasis is being given on organic agriculture by using
botanicals, biocontrol agents and different organics. Although, attempt has been made to manage the various fungal
diseases of paddy with these agents, not much work have been done on brown spot of paddy. Therefore, an attempt has
been made to study the efficacy of plant extracts, organics and biocontrol agents against brown spot disease.
483
The Potentiality of different Plant Extracts/ Bioagent and Organics in Reducing Incidence of Brown Spot of Paddy
in Organic Cultivation under Pot Condition
The best treatment (s) against brown spot of paddy were evaluated further in pot condition.
Preparation of Suspension of Pathogen for Pot Experiment
Spore suspension of Bipolarisoryzae was prepared from 15 days old culture grown in PDA medium. The spores
were suspended in sterile distilled water and the concentration was adjusted to optical density (OD) @ 680 nm in
spectrophotometer to obtain a fungal population of 1.0 x 107 CFU/ml.
Seed Treatment with Four Promising Treatments
Rice seeds (var. Prafulla) were soaked in the different concentrations (10, 20 and 30%) of effective treatments for
24 hrs. After soaking, the treated seeds were dried in shade for 12 hrs and were sown in pots with four replications.
Disease Record
Symptoms of brown spot disease of paddy were observed critically during the crop season. The observations were
made at 45 DAS and 90 DAS and disease incidence (D I) expressed in terms of percentage was calculated as follows :
D.I. (%) =
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484
Radial
Growth
(mm)
14.80
Neem( Azadirachtaindica)
17.00
81.00
Karanja ( Pongamiaglabra)
21.00
76.53
26.60
70.27
Wood ash
28.20
68.49
32.80
63.35
53.00
40.78
42.20
52.84
Thuja ( Thujaoccidentalis)
66.70
25.47
10
Vermicompost
45.60
49.05
11
Rice gruel
56.00
37.43
12
Trichodermaviride
29.00
67.59
13
Carbendazim (0.01%)
0.00
100.00
Absolute control
SEd ()
CD
89.50
0.92
1.55
00.00
Treatments
T
T
T
T
T
T
T
T
T
T
T
T
T
T
14
0.05
Per cent
Inhibition
Over Control
83.46
485
Tulsi @ 0.10%
Radial
Growth
(mm)
52.70
Tulsi @ 0.25%
26.00
70.94
Tulsi @ 0.50%
23.40
73.85
Neem @ 0.10%
56.70
36.64
Neem @ 0.25%
34.60
61.34
Neem @ 0.50%
32.20
64.02
Karanja @ 0.10%
58.20
34.97
Karanja @ 0.25%
35.70
60.11
Karanja @ 0.50%
34.60
61.34
10
Papaya @ 0.10%
64.00
28.49
11
Papaya @ 0.25%
38.80
56.64
12
Papaya @ 0.50%
37.20
58.43
13
Carbendazim
0.00
100.00
Absolute control
SEd ()
CD
89.50
0.26
0.48
00.00
Treatments
T
T
T
T
T
T
T
T
T
T
T
T
T
T
14
0.05
Percent
Inhibition
Over Control
41.11
The Potentiality of different Plant Extracts in Reducing Incidence of Brown Spot of Paddy in Organic Cultivation
under Pot Condition
Result revealedthat all the extracts Table 3, irrespective of different concentrations could reduced Percent
disease incidence with increased PDC of brown spot of rice under pot condition, but efficacy was lower than chemical
(Carbendazim 0.2%). Maximum PDI of brown spot of rice was recorded in inoculated control and minimum PDI with
increased PDC recorded with extracts of tulsi (Ocimum sanctum) @ 30 per cent which was at par with extract of tulsi
(Ocimum sanctum) @ 20 per cent followed by same extract @ 10 per cent and extract of neem (Azadirachtaindica) extract
@ 20 per cent. Analysis of variance showed that among the extracts used, tulsi (Ocimum sanctum) extracts was found to be
higher in reduction of PDI of brown spot of rice which was followed by neem (Azadirachtaindica), karanja
(Pongamiaglabra) and papaya (Carica papaya), respectively. Among the concentrations, 30 per cent showed significantly
higher reduction of PDI of brown spot of rice than the other two concentrations. The reason might be due to anti-fungal
substances produced by plant extracts which directly or indirectly affected on disease suppression. Results similar to
present investigations were reported by several workers. Tiwari and Nayak (1991), reported that leaf extracts of Ocimum
sanctum was quite effective in reducing the growth of Rhizoctoniasolani in vivo. Singh et al. (2010) reported that foliar
spray of aqueous extract of neem cake showed antifungal efficacy against powdery mildew of balsam. Sunder et al. (2010)
reported that botanicals neemazal, neem gold, garlic reduced leaf spot phase and stalk rot incidence of brown spot of rice.
Prince and Prabakaran (2011) also reported the antifungal activity of eight different medicinal plants namely Aloe vera,
Ocimum
sanctum,
Cenetellaasiatica,
Piper
betle,
Calotropisgigantea,
Vitexnegundo,
Ocimumbasilicum
and
Azadirachtaindica against Colletotrichumfalcatum. Akila (2009) reported that leaf extract of neem was highly effective in
reducing the grain discoloration of rice which was at par with carbendazim.
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Yield
(g/hill)
13.82
13.51
15.73
12.02
12.09
12.16
11.23
11.51
11.76
10.66
10.98
11.01
16.52
10.22
0.34
0.71
The Effect of different Plant Extracts against Brown Spot of Paddy in Increasing Yield under Pot Condition
Yield of the crops after harvesting of paddy was recorded i.e. 150 DAS. The plants were weighed on weighing
balance @ gram per hill. The highest yield (15.73 g/hill) was recorded in Tulsi (Ocimum sanctum) @ 30 per cent
concentration. While lowest yield (10.66 g/hill) was recorded in papaya (Carica papaya) @ 10 per cent concentration
which was at par with carbendazim @ 0.2 per cent. A careful study of literature revealed that no observation on highest
yield of paddy treated with tulsi extract was recorded against plant pathogens. Sunder and Singh (2010) reported that neem
leaf extract could increased grain yield of rice.
CONCLUSIONS
Although, seed treatment with fungicide (0.2%) was also effective in reducing the incidence of the disease, its
application alone should preferably be avoided because chemical control measures are not eco-friendly for their potentially
hazardous toxic effects.
The present investigation have shown encouraging result in using plant extract as ecofriendly measure against
Bipolarisoryzae causing brown spot of paddy but their efficacy in field condition under rainfed and irrigated condition
should be further evaluated.
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