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9.1 Derive the relationship between the overall mass transfer coefficient for gas phase K G and the
individual mass-transfer coefficients, kL and kG. How can this relationship be simplified for
sparingly soluble gases?
Given:
a. Overall mass transfer coefficient for gas phase, KG
b. Individual mass transfer coefficients, kL and kG
Required:
a. Derive the relationship between KG, and kG and kL
b. How can the relationship be simplified for sparingly soluble gases?
Solution:
Expressing the molar flux in terms of overall driving force for mass transfer and an
overall mass transfer coefficient,
P A
P A , G
N A =K G
c Ac A , L
N A =K L
where KG is termed the overall mass transfer coefficient based on gas phase driving
forces. In an equivalent manner, we also can write
Using Henrys Law,
mc A
P A , G=
where m is the Henrys law constant.
We can rewrite the first two equations as,
N
P A , GP A ,i= A
kG
c A , ic A , L =
NA
kL
c g
Eq. (9.25)
k L =0.31 N Sc 3 (
Eq. (9.26)
N Sh=0.31 N Sc 1/ 3 N Gr 1/3
1 /3
is the same as
b) Prove that
2
1 /3
2 D AB
3 c g
Eq. (9.27) k L = D + 0.31 N Sc ( 2 )
32
Eq. (9.28)
is the same as
Solution:
a.)
N Sh=0.31 N Sc
1/ 3
N Gr
1/3
2
3
k L =0.31 N Sc (
c g
1 /3
Substituting Eq. (9.21), Eq. (9.22) and Eq. (9.23) into Eq. (9.26):
k L D32
c 1/ 3 D332 c g 1 /3
=0.31(
) (
)
D AB
c D AB
2c
c 1 /3
c 1 /3 g 1/3 1 /3
k L =0.31 1/ 3
c D AB2 /3
2c /3
k L =0.31
1/ 3
1/3
c
D AB
1 /3
1/3
1 /3
D32
D AB
Combining similar
terms
2 /3
1/3
1 /3
k L =0.31 1/ 3 c 2 /3 c
c D AB
1 /3
1/3
Multiplying
1/ 3
c 2 /3
c 1/ 3 g1 /3 1 /3
k L =0.31 2/ 3
c D AB2 /3
2/ 3c
1/3
)( c2/ 3 )
2/ 3
c
c
c
c D AB
k L =0.31
2
k L =0.31 N Sc 3 (
c g 1 /3
)
2
k L =0.31 N Sc 3 (
c g 1 /3
)
2
b.)
N Sh=
2 D AB
+0.31 N Sc 1/ 3 N Gr1 /3
D 32
2
k L =2.0+0.31 N Sc 3 (
c g
1/ 3
Substituting Eq. (9.21), Eq. (9.22) and Eq. (9.23) into Eq. (9.28):
k L D32
c 1/ 3 D 332 c g 1/3
=2.0+0.31(
) (
)
D AB
c D AB
2c
D32
D AB
1/ 3
1/ 3
1 /3
c
1/ 3
k L =2 AB + 0.31 1/ 3 c 2 /3 c
D32
c D AB
k L =2
D AB
c
+ 0.31 1/ 3
2 /3
D32
c D AB
1 /3
1 /3
1 /3
1 /3
g
2 /3
c
g
D AB
c1 /3
c 1/ 3 g1 /3 1 /3
k L =2
+ 0.31 1/ 3
D32
1
c D AB2 /3
2/ 3
k L =2
D AB
c
+ 0.31 2/ 3c
2 /3
D32
c D AB
Combining similar
terms
1/ 3
c
c D AB
D AB
k L =2
+ 0.31
D32
2
1 /3
D AB
3 c g
k L =2
+ 0.31 N Sc (
)
2
D 32
2
1 /3
D AB
3 c g
k L =2
+ 0.31 N Sc (
)
D32
2
1 /3
g
2/ 3
c
1 /3
Multiplying
3
1/
1/3
c
c
)(
)
2/ 3
c
2/c 3
Power Requirement
Gas Hold-up
Sauter-mean Diameter
Interfacial area
Volumetric mass-transfer coefficient
Given:
DT = 1.22 m
W = 1/10 DT
DI = 0.36 m
N = 4.43 rps
Q = 0.0217 m3/s
Required:
a.
b.
c.
d.
e.
Pm
H
D32
a
kla
Solution:
a. Nre = (997.08)(4.43)(.36)^2
8.904 x 10^-4
Nre = 642,915.034 > 10,000
d. Interfacial Area:
a =6H/ D32
a= 98.5447 /m
Kl = 4.58x10^-4 m/s
Pm= 1341.3218 W
b. v= (/4)(1.22)(1.22)^2
v= 1.43 m3
Vs= (4x .0217) / (1.22)^2
= 0.0186 m/s
<
0.02 m/s
Using equation 9.48 of James Lee
H = 0.0790
c. Using equation 9.42
D32= 4.8132 x 10^-3 m
D32= 4.8132 mm
Kla = 0.0451 /s
9.5 Estimate the volumetric mass-transfer coefficient kLa for the gas-liquid contractor described
in Problem 9.4 by using a correlation for kLa and compare the result with the experimental value.
Given:
Reactor volume, v= 1.43m3
Vs= 0.0186 m/s
PM= 1342 Watts
Required:
kLa (using equation 9.71 by James Lee)
% kLa compared with experimental value
Solution:
1342
kLa = 0.026( 1.43 )0.4 (0.0186)0.5 = 0.0548 s-1
Experimental
( 0.05480.0451 ) x 100
= 17.7007% error
(0.0548)
N 3 D5I
( )
Can you determine the power consumption and impeller speed of a 1,000-gallon fermenter
based on findings of the optimum condition from a one-gallon vessel by using the same fluid
system? Is your conclusion reasonable? Why or why not?
Given:
P mo
ND2I
=f
N 3 D5I
( )
VP =1000 gallons
Vm=1 gallon
Required:
Can the power consumption and impeller speed of VP be determined on findings of the
optimum condition from Vm by using the same fluid system? Why?
Solution:
VP
=1000
Vm
The scale ratio is
1
DI , P
=1000 3 =10
DI,m
To achieve dynamic similarity, the three numbers for the prototype and model must be
equal
Pmo
P mo
=
3
5
N DI P N 3 D5I m
] [
[ ] [ ]
ND 2I
ND 2I
=
P
( Pmo )p =10 [ P mo ] m
P =m
[ ]
NP
Nm
P m ;
[]
P m ;
[]
1
=
m 31.6
qo
Concentration, mmol/L
Escherichia Coli
0.5 mmol(gdw)-1h-1
0.0082
Vitisvinifera (grape)
0.6 mmol(gdw)-1h-1
0.055
3.0x10-10 mmol(gdw)-1h-1
0.020
a.Estimate the kLa requirement to achieve cell concentrations of 25 gram dry weight/L for E.
Coli and V. Vinifera and 3.0x109 for CHO cells, while maintaining dissolved oxygen
concentration above critical. The oxygen solubility in the media used for the cultures is 7.2x10 -3
kg/m3
b. The relationship between kLa and the power input to a 1-m3 stirred bioreactor is kLa
Pt
Vl
0.5
, compare the bioreactor power requirements for culture of the three different cell types
Pt
b.) kLa= k ( Vl )
0.5
KLa =
C C AL1
ln ( AL
)
C ALC AL2
t 2t 1
7850
)
7866
( 205 ) s
ln (
KLa =
= 0.056/s
a)
1
RV L
([ F TP ) i( F TP ) o]
g
Ag
Ag
0.201 x 1.48
1
8.91 x
( ( 30+273 ) K )
60
0.23 L
(0.2099 atm)
s
( 22+273 ) K
1
0.08205 L. atm
(20 L)
mol . K
((
1
( 1.6357 x 104 1.4572 x 104 ) =1.0878 x 105 mol
L.s
0.08205 (20)
Because of steady state the rate of oxygen transfer is equal to the rate of oxygen uptake
by the cells; the volumetric rate of oxygen uptake by the culture is 1.0878x10-5 mol/ L.s
b) Assume that the gas phase is well-mixed so that the oxygen concentration in the bubbles
containing the liquid is the same as the outlet gas, that is 20.1%. As the difference in the
composition of the gas phase to be constant throughout the fermenter.
The solubility of oxygen in H 20 @30oC and 1atm air pressure is 8.05x10 -3 kg/m3.
Determine the solubility at the fermenter operating pressure of 1.48 atm and gas phase
oxygen mole fraction of 0.201.
C*AL,2
PT 2 Y AG 1
PT 1 Y AG 2
C*AL,1
CAL in the fermenter is 82% of the oxygen solubility at 30oC and 1.48atm air pressure
CAL= 0.820
32 g
)
mol
3
0.0114 g 9.77 x 10 g
L
L
5
1.1 x 1 0 mol / L . s (
KLa =
A strain of Azobacter vinelandii is cultured in a 15m 3 stirred fermenter for alginate production.
Under current operating conditions, KLa is 0.17 s-1. The solubility of oxygen in the broth is
approximately 8x10-3 kg/m3.
a. The specific rate of oxygen uptake is 12.5 mmol/g-hr. What is the maximum cell
concentration supported by oxygen transfer in the fermenter?
b. The bacteria suffer growth inhibition after copper sulphate is accidentally added to
the fermentation broth just after the start of the culture. This causes a reaction in the
oxygen uptake rate to 3 mmol/g-hr. What maximum cell concentration can now be
supported by oxygen transfer in the fermenter?
Solution:
a) Xmax =
0.17 8 x 1 0 kg
(
)
3
s
m
mmol 1 hr
1 mol
32 g
12.5
g . hr 3600 s 1000 mol 1 mol
( )
( )(
)(
1 kg
)( 1000
g)
12240 g
3
m
b) Assume that addition of copper sulphate does not affect C*AL of KLa
0.17 8 x 1 03 kg
(
)
s
m3
Xmax=
mmol 1 hr
1 mol
32 g
3
g . hr 3600 s 1000 mol 1mol
( )
( )(
)(
1 kg
)( 1000
g)
51000 g
3
m
Fr =
2 Di
g
= 1.25s-1
1.25 s1 2 (0.5 m)
= 0.0796
0.5
DI
DT
( )
Fr 0.5 = 0.2
0.5 m
1.4 m
0.5
1 min
Ni = 45 /min ( 60 s ) = 0.75s-1
1 2
0.75 s (0.5 m)
Fr =
Di
g
= 0.0287
*Flooding-loading transition
Flg = 30
DI
DT
3.5
( )
Fr
= 30
0.5 m
1.4 m
3.5
= 0.0234
*At reduced stirrer speed, maximum air flow rate can be handled without impeller
flooding as operating flow rate (0.28m3/min) is greater than this. The impeller is
FLOODED.
By performing a carbon, nitrogen, hydrogen, and oxygen balance, determine the chemical
composition of the cells.
Solution:
By performing a carbon, hydrogen, nitrogen and oxygen balance, determine the element
composition of the cells.
100C6H12O6 + 11.2 NH3 13CaHbOcNd + 56C4H10O (butanol) + 22C3H6O(acetone) +
0.4C4H8O2(butyrate) + 14C2H14O2 (acetic acid) + 221CO2 +135H2 + 0.7 C2H6O(ethanol)
* where CaHbOcNd represents elemental composition of clostridium cells
Carbon Balance:
100(6) + 11.2(0) = 13(a) + 56(4) + 22(3) + 0.4(4) + 14(2) + 221(1) + 0.7(2) : a = 4.46
Hydrogen Balance:
100(12) + 11.2(3) = 13(b) + 56(10) + 22(6) + 0.4(8) + 14(14) + 135(2) + 0.7(6) : b = 16.02
Oxygen Balance:
100(6) + 11.2(0) = 13(c) + 56(1) + 22(1) + 0.4(2) + 14(2) + 221(2) + 0.7(1) : c = 3.88
Nitrogen Balance:
11.2(1) = 13(d) : d = 0.86
d=
0.2
(0.45)
hr
12 g/mol
45% =
g cell biomass
g Carbon
(100)
MW cell biomass
C
MWcell =
12(100)
=26.67
45
a= Qglucose =
Y X /S
0.2/hr
g dry cell
90
g glucose
0.043
b= Qglutamate =
147
g glutamate
g cell . hr
g
glutamic acid
mol
Elemental Balance:
C: 6a + 5b = d + e
H: 12a + 9b = d + 2f
O: 6a + 4b + 2c = d + 2e + f : 2c=2e : 6a + 4b = d + f
N: b = d
=
b
d
2.925 x 104
7.5 x 1 03
Using H and O:
12a + 9b = d + 2f
-2
(6a + 4b = d + f)
b= d - 2d
= 0.0390
14.124 =
2.925 x 10 +0.015
3
7.5 x 10
+ 16 : = 0.7825, = 1.604
Using equation of O:
6a + 4b = d + f ; f = 6a + 4b d
f= 6(2.22x10-3) + 4(2.925x10-4) 0.7825(7.5x10-3)
f = 8.6213x10-3
Using equation of C:
6a + 5b = d + e ; e = 6a + 5b d
e = 6( 2.22x10-3) + 5( 2.925x10-4) 7.5x10-3
e = c = 7.2825x10-3
* aC6H12O6 + bC5H9O4N + cO2 dCHOO + e CO2 + fH2O
Answer:
2.22 C6H12O6 + 2.925C5H9O4N + 7.2825 O27.5 CH1.604O0.7825O0.0390 + 7.2828 CO + 8.6213H2O
D=0.3 hr-1
m=0.4 hr-1
Ks=2 kg/m3
CSO=50 kg/m3
0.5 g cell (dry )
YX/S=
g glucose
N=60 rpm
= 1200 kg/m3
=0.02 Pas
Cell formula: CH1.8O0.5N0.2
Required: Mass transfer limited or Biochemical reaction limited
Soln:
H
=2.5
DT
H = liquid height
DT = tank diameter
2
2
3
V T = D T H = DT ( 2.5 D T )= ( 2.5 ) ( DT )
4
4
4
VT = 50 m3
50 m = (2.5 ) (D T 3 ) ;
4
3
DT = 3
50 m3
( 2.5 )
4
DT = 2.9420 m
0.02 Pa s
= =
=1.67 105 m3 / s
3
1200 kg /m
DI =
DT
=3 ; DI = impeller diameter
DI
DT 2.9420 m
=
=0.9807 m
3
3
[ ]
N D I2
'
Reynol d s Number , Re =
=
60 rpm
0.02 Pa s
57706.3494
Re 10000; Np = 6 (p. 258, James Lee)
N = (60/60) rps
For a flat-blade turbine at Re 10000
3
kg 60
Pmo=N P N 3 DI 5=6 1200 3 ( rps) (0.9807 m)5
m 60
0.115
DI
DT
( )
[ ] [ ] [ ] [ ]
P
D
log 10 m =192 I
Pmo
DT
D I2 N
DI N 2
g
1.96
Q
N D I3
James Lee)
3
Q=
0.5 m O 2
3
m broth min
( 50 m3 0.60 ) =0.25 m 3 / s
60 2
60
1.96
(0.9807)
( 0.9807
2.9420 )
0.25 m3 /s
60
( )(0.9807)3
60
Pm
0.9807 m
=192
2.9420 m
2 ( 6531.5066 W )
log 10
4.38
60
rps )
60
1.67 105
(0.9807)2 (
Pm = 6191. 1993 W
For computation of kLa, superficial velocity
1 min
0.5 m3 air
60 sec
V s=
Q
=
S
m3 media min
(2.9420 m)2
4
( 50 m3 0.60 )=0.0368 m/ s
0.115
k L a=2.0 10
6191.1993 W
3
50 m (0.60)
0.70
m 0.20
(0.0368 ) =0.0431 s1
s
Assume: Solution of H2SO4 @ 2.0 mols/L, find the corresponding oxygen solubility from
Table 9.2 of Biochemical Engineering by James Lee, p. 262
CO2 = 1.02 mmol/L
Use the solubility of O2 from Table 9.2 to determine the Henrys constant and the
equilibrium concentration of O2 at that condition.
1 atm
atm L
HO =
=0.9804
1.02 mmol/L
mmol
2
C L =
0.21 atm
mmol
=0.2142
atm L
L
0.9804
mmol
](
)[
][
][
][
1000 L
kg
=1.0635 3
3
1m
m hr
[ ]
Where:
C, H, O is the number of atoms present in the carbon source
C, H, O, N, - is the percent carbon, hydrogen, oxygen and nitrogen respectively in the
cell
ZO =
2
32 ( 6 ) +8 (12)16(16)
+ 0.01(32.52)0.0276(48.78)+0.01714 (11.38)0.08(7.32)
0.5(180)
Z O =0.7656
2
gO2
g dry cell
Z O x m=0.7658
2
g O2
kg
22 3
g dry cell
m
Thus, it is Mass transfer limited because theres very little oxygen that is being transferred or dissolved in
broth.
In an oxygen absorption study, the following data were obtained on the oxygen transfer capacity
of an air diffusion unit.
Q= 9.439 m3/s
T= 12C
HL= 4.27 m
Cs, kg/m3
0.6 x10-3
1.6 x10-3
3.1 x10-3
4.3 x10-3
5.4 x10-3
6.0 x10-3
7.0 x10-3
C sm=C s
Pb O t
+
29.4 42
For Csm,
C sm=1.08 x 102
Time, min
3
6
9
12
15
18
21
0.01178
0.01078
9.28 x10-3
8.08 x10-3
6.98 x10-3
6.38 x10-3
5.38 x10-3
kg
60 min
kg
=0.0216 3
m min 1 hr
m hr
3
Determine the interfacial area for the calculation of the mass transfer coefficient, kL
A 6Q HL
=
; V T = DT 2 H L
V dBV B V T
4
Where :
A/V is the interfacial area
dB is the air bubble diameter
VB - is the air bubble velocity
6 ( 9.439 m3 ) (4.27 m)
=9.7114 m1
( 2.5 103 m ) 0.3 m (33,201.97 m3)
s
For kL,
k L=
kLa
2.7 /hr
m
=
=0.2677
A /V 9.7114 /m
hr
B.
( qv )
( qv )
k L a(12 )
1220
1.02
2.6/ hr
3.046
=
1220
hr
1.02
(eq.1)
(20 )
=k L a(32 ) (C s ,32 C L, 32 )
(eq.2)
(32 )
2
=k a
( C C L,20 ) = hr ( 1.07 10 0 ) 3
v (20 ) L (20 ) s
m
()
q(20 )=
3.046
( 1.07 1020 ) kg3 ( 28.317 m 3 )=0.923 kg
hr
hr
m
1.5 102 )
q( 32 )@ 80 eff =0.80
kg
kg
=6.2778
3
hr
m
HL= 1.2 DT
OTR= 0.25 kmol/m3hr
V2= 60 m3
VL= 0.018 m3
Determine the tank diameter, DT, and impeller diameter, DI, for each conditions.
V 1=
2
2
3
DT H L = ( DT ) ( 1.2 DT )=0.3 DT
4
4
1
1
3
( ) [
V1
DT =
0.3
1
0.018 m
=
0.3
3 1
3
=0.267 m
1
1
D I = DT = ( 0.267 m) =0.089 m
3
3
1
pg.274)
H L =1.2 DT =1.2 ( 0.267 m )=0.32 m
1
0.018
60 m
V 2 13
0.03
DT =
=
0.3
0.3
2
( )
1
3
=3.36 m
1
1
D I = DT = ( 3.36 m )=1.12 m
3
3
2
4
m3 3600 sec
3 1 04
sec
1hr
Q
V s= =
1 atm+ 1 atm+
PO =
HL
10.3 m H 2 O
2 atm
1 atm+ 1 atm+
( 0.21 )=
0.32m H 2 O
10.3m H 2 O
2 atm
( )
( )
( )
0.95
Then determine the ungassed power, Pmo, for two sets of impeller, Np= 2x6=12
P g
N N i 3 D i5
N p= mo3 c 5 ; Pmo = p
gc
N i Di
0.089 m
N i 3
kg
12 1200 3
m
N p N i3 Di5
Pmo=
=
gc
0.1 hP=0.5
N i=18 rps
( 0.108 103 N i3 )
( 0.089 m )3
(3 104 m3 / sec)0.56
60 sec
=1080 rpm
1 min
[ ]
Pm
0.10
=
=0.1587
Pmo 1 0.630
1
M
N 3 DI 5
V1 1
1
] [
=
model
1
M
N 3 DI 5
V2 2
2
prototype
*Accdg. To Ghose
N 13 D I 5
V
N2 =
; N 2=N 1 2
V1
V1
DI 5
V2
3
1
3
[ ][ ]
DI
DI
5
3
3
DI
N1
=
N2
DI
[ ][ ]
2
[ ]
DI
; N 2=N 1
DI
2/ 3
N 2=1080
1
3
[ ][
60
0.03
0.089
1.12
5
3
,rpm
N 2=199.8575 rpm
[ ] [ ]
DI
0.089
N 2=N 1
=720
DI
1.12
1
N 2=57.2143 rpm
2/ 3
[ ]
DI
N 2=N 1
DI
=720
0.089
1.12
2/ 3
N 2=3895.39 rpm
[ ]
2
2 /3
[ ]
m
N 2=3895.39 rpm
Or
(N DI ) p=1.7( N D I )m
K La C al
qo
2
10 kg
3
. The specific oxygen uptake is 15 mmol g/h. What is the
m
1
1
32 g
1 kg
(
)(
)(
)
(15 mmol
)(
)
gh 3600 s 1000 mmol 1 mol 1000 g
Xmax 15937.5
g
g
=15.94
3
L
m
V = 80 m3
Vs = 2.6 m/min
Required:
Gas Hold-up, Ho
Solution:
From equation;
P
V
Vs
(
0.4
Where; P = power in hp
V = ungassed liquid volume in m3
Vs = gas superficial velocity in m/h
Solving for H:
18
80
2.6 x 60 min /h
H = 0.6 m
The gas hold up can be defined by the above definition using the gas height per volume,
where H = 0.6 m for aeration
Ho =
Vg
V g+V L
Ho = 8.5%
0.6
0.6+6.5
= 0.085
kL = 0.002(Pg/V)0.7(Us)0.5
1.174 = 0.002 (Pg/V)0.7(41.45)0.5
For the gassed power per unit volume (Pg/V) is 630.7 W; thegpassed power, Pg, was 0.15hp.
Since the flow regime is turbulent, the power number obtained from figure 6.6, Power number
versus Reynolds number, Re, reads Pno = 6. For two sets of impellers, Np = 2(6) = 12
`
Np = (P1 gc)/(N13Di5)
P1 = (12 x 1200 x N13 x 0.1925)/9.81 = 0.383 N13 W = 5x10-4 N13 hp