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Original Article
Abstract
Limited by the sensitivity of laboratory techniques, conventional human DNA analysis of touch DNA on frequently used items and prints does
not always provide satisfactory results. In this study, microbiome DNA on personal computers, cell phones, and palm prints was analyzed and
compared. After sample collection, DNA extraction, polymerase chain reaction amplification, library preparation, and sequencing, data were
analyzed using the QIIME 1.8.0 software. Weighted unifrac distance between the right palm skin and the right side of a keyboard, the right
palm skin and the mouse, and the left side of the keyboard and the left palm skin was 0.258850, 0.265474, and 0.214098, respectively. Even
after palm prints were left for 1week, microbial community structures were still quite similar to those of samples collected from the palm
skin on the day they were left(weighted unifrac distance was 0.270885).
Key words: Cell phone, keyboard, metagenomics, palm prints
Introduction
Website:
www.jfsmonline.com
DOI:
10.4103/2349-5014.184190
74
How to cite this article: Yao X, Liu W, Han J, Pei G, Tong Y, Luo Y. Analysis
of Microbiome DNA on Frequently Touched Items and from Palm Prints.
J Forensic Sci Med 2016;2:74-7.
2016 Journal of Forensic Science and Medicine | Published by Wolters Kluwer - Medknow
[Downloaded free from http://www.jfsmonline.com on Friday, August 19, 2016, IP: 120.169.255.129]
Yao, etal.: Analysis of Microbiome DNA on Frequently Touched Items and Palm Prints
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[Downloaded free from http://www.jfsmonline.com on Friday, August 19, 2016, IP: 120.169.255.129]
Yao, etal.: Analysis of Microbiome DNA on Frequently Touched Items and Palm Prints
76
[Downloaded free from http://www.jfsmonline.com on Friday, August 19, 2016, IP: 120.169.255.129]
Yao, etal.: Analysis of Microbiome DNA on Frequently Touched Items and Palm Prints
This work has received funding from the State Key Laboratory
of Pathogen and Biosecurity (xxhz201510), and Shanghai
Research Institute of Criminal Science and Technology
(2014XCWZK14).
Conflicts of interest
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