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INSTRUMENTS

Lab 153
1. Thermo Scientific Kingfisher flex 96 DW

Offers highly versatile, automated magnetic-particle


processing for DNA/RNA, protein or cell purification from
virtually any source.
Yields high-speed purification of nucleic acids, proteins
and cells.
Up to 8 plates can be simultaneously on the turntable.
However, during one sample processing, the protocol
enables the use of more than 8 plates in total. One tip
comb contains 24 or 96 tips used for processing 24 or
96 samples at a time.
There are 4 types of plates available for use:
o Kingfisher 24 deep well platehas the capacity to
hold between 200l and 5ml of liquid sample.
o Microtiter deep well 96 plate has the capacity to
hold between 50l and 1000l of liquid sample.
o Kingfisher 96 platehas the capacity to hold
between 20l and 200l of liquid sample.
o PCR platehas the capacity to hold between 20l
and 100l of liquid sample.
2. Labnet Problot 12S hybridization oven

provides an extremely precise and uniform temperature


environment.

ideal for growth of cultures and other applications


requiring tightly controlled incubations.
The rotisserie in the ProBlot 12S will hold 12 large or 24
small bottles as well as 50 ml tubes.
The integrated shaker in the ProBlot 12S has an orbit
of 10mm and a maximum speed of 300 rpm.
The shaker operation is controlled by a 2 hr. timer with
a hold position.
The oven is provided with a rotisserie for 35 mm
diameter bottles and 50 ml disposable tubes, a drip
tray, two large bottles and a pack of hybridization mesh
In addition to the rotisserie, the ProBlot 12S has an
orbital shaking platform in the bottom for bulk washing
or to use the oven as an incubator shaker.
3. Applied Biosystems 3500 Genetic Analyzer

Supports the following applications:


o DNA sequencing:
Direct sequencing for mutation detection
Comparative sequencing with and without
references
Microbial sequence identification
o HID (human identification) analysis:
Microsatellite
AFLP (amplified fragment length
polymorphism)
SNaPshot kit
LOH (loss of heterozygosity)
MLPA (Multiplex ligation-dependent probe
amplification)
o Fragment analysis:
Forensic DNA casework

Databasing
Paternity testing
Biological samples that can be analyzed include:
tissues, body fluids, infectious agents, blood, RNA, DNA,
and more.
For a 96-well plate, use 10 L of mixture per well. For a
384-well plate, use 5 L of mixture per well. Add the
mixture to wells A1-H1
uses capillary electrophoresis technology with 8- or 24capillaries
is fully automated, from sample loading to primary data
analysis, for sequencing and fragment analysis
For most applications, the sample is denatured so that
only single-strand DNA is present.

4. Agilent 2100 Bioanalyzer (Electrophoresis)

Types of samples that can be analyzed include:


o RNAanalysis include: RNA integrity,
quantification and sizing
o DNAanalysis include: DNA sizing and
quantification
o Proteinsanalysis include: protein sizing,
quantification, and purity
o whole cells
can analyze 10, 4l samples in less than 30 minutes
The total number of samples that can be measured
varies with the type of assay selected. With DNA and
RNA Nano assays, 12 samples may be run; with RNA

Pico assays, 11 samples may be run; and with Protein


assays, the maximum number of samples is 10.
Minimal sample consumption is 1-4l
As little as 200pg/l RNA is required per analysis
The applications that can be performed include the
following:
o DNA fragment analysis
Sample QC in next generation sequencing:
- Size distribution analyses
- QC of PCR-amplified libraries
PCR validation and impurity check
Restriction digestion analysis
Food analysis
o Protein applications
Optimization and monitoring of protein
purification
Protein expression
Protein production
Food analysis
o Sample QC in gene expression
QC prior to gene expression studies utilizing
microarrays and qPCR
Transcriptome profiling with RNA sequencing
QC of small RNA fractions for miRNA research
or Small RNA sequencing
QC of plant RNA or bacterial RNA
o Simple flow cytometric analysis of cells
Protein expression monitoring
Transfection efficiency monitoring
Apoptosis detection
Gene silencing in cell culture

5. Qiagen Beadmill Tissue Lyser II

simultaneously disrupts multiple biological samples


through high-speed shaking in plastic tubes with
stainless steel, tungsten carbide, or glass beads.
up to 48 or 192 samples can be processed at the same
time
The following are TissueLyser Adapters and Jar Sets
used:
o TissueLyser Adapter Set 2 x 24allows disruption
of 48 samples in parallel using standard 2ml
microcentrifuge tubes
o TissueLyser Adapter Set 2 x 96allows disruption
of 192 samples in parallel using Collection
Microtubes (racked) and Collection Microtube Caps
o Grinding Jar Set, S. Steelallows disruption of 2
large samples in parallel using stainless steel
grinding balls
o Grinding Jar Set, Teflonallows disruption of 2
large samples in parallel using Teflon grinding balls
A wide variety of biological samples can be processed,
including: human, animal, and plant tissues as well as
yeast and bacteria
Disruption time ranges from 10 seconds up to a
maximum of 99 minutes
is intended for molecular biology applications such as:
o Purification of DNA, RNA, and proteins
o Nucleic acid and protein assays
o microRNA research and RNAi
o Automation of sample and assay technologies

6. Agilent Technologies 7890A GCMS System

An analytical instrument that measures the content of


various components in a sample
Performs advanced separation capabilities features
such as:
o EPA 8270
o Pesticides in Milk Extract
o Impurities in Hydrocarbons
o Rapid Drug Screening
o Analysis of Refinery Gases
o Comprehensive GC Flow Modulation
Up to 150 samples can be placed in 3 vial racks
You can place up to 16 vials, a solvent A, a solvent B,
and a waste bottle in the standalone turret. If you use
the transfer turret, you can load 3 sample vials and the
solvent and waste bottle capacity increases to 15
Identifying and quantifying volatile organic compounds
in:
o Liquidspesticides, mineral oils such as: waste
oils and transformer oils, fatty acids,
o GasesMethane, Carbon Dioxide,
o Solidsplastics, sediments, trace compounds,
PCBs (Polychlorinated biphenyls), solid waste,
separates mixtures into individual components using a
temperature-controlled capillary column.

7. Thermo Scientific MaxQ 4450 shaker

ideal for incubating a small number of vessels and are


used for cell culture, solubility studies, extraction
procedures, protein expression and many other
applications.
Used in the incubation of samples that has to be shaken
while being stored.
8. Applied Biosystems Veriti 96 Well Thermal Cycler

allow for precise PCR optimization and enable you to


run up to 6 independent assays
Reduced PCR cycling time when using faster ramp rates
9. Applied Biosystems ViiA 7 Fast 96

Uses fluorescent-based polymerase chain reaction


(PCR) reagents to
provide:
o Quantitative research detection of target nucleic
acid sequences (targets) using real-time analysis.

10.

o Qualitative research detection of targets using


post-PCR (endpoint) analysis.
o Qualitative analysis of the PCR product achieved
by melt curve analysis that occurs post-PCR.
o accurate and sensitive data analysis
The ViiA7 comes equipped with a 96 well-Fast block, a
384 well block, and a TaqMan Array Card.
Run Time ~35 min for 96/384-well blocks, using Applied
Biosystems Fast Master Mix
Supported Blocks and Volume 96-well standard (10100
L reactions)
96-well Fast (1030 L reactions)
384-well (520 L reactions)
TaqMan Array Micro Fluidic Cards (~1 L reactions)
Excitation/Detection Wavelengths 6 excitation (450670
nm)
Thermo Scientific Nanodrop 2000

Spectrophotometer
Only requires 0.5 2 L of your sample to measure
samples with high accuracy and reproducibility in less
than 15 seconds
employs surface tension to hold the sample in place
between two optical fibers
No dilutions required, even for highly concentrated
samples, therefore can measure sample concentration
up to 200 times more concentrated than samples
measured using the standard cuvette.
Wide spectral range (190-840nm) for measuring a
variety of samples types:
o Peptides (205nm)
o DNA and RNA (260nm)

Purified protein (280nm)


Toxicology assays and industrial dyes (490nm)
Gold nanoparticles (520nm)
Colorimetric protein assays (BCA 562nm, Bradford
595nm, Modified Lowry 650nm, Pierce 660 660nm)
o Optical Density measurements (600 nm)
Patented sample retention system automatically
optimizes path length to accommodate low and high
concentrations (2 to 15,000 ng/L dsDNA)
Calculates sample purity ratios (260/280 nm and
260/230 nm)
Pre-configured methods for DNA, Protein A280,
Microarray, Protein and Labels, Pierce 660, Bradford,
BCA, and Lowry
Fast measurement time of less than 5 seconds, 10 -15
seconds from sample pipette to wipe
User friendly software that includes custom methods
and data export capabilities
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