JSA Ira

Reference Number-
JOB SAFETY / HAZARD ANALYSIS

&
RISK ASSESSMENT FORM
UNIVERSITY OF KUALA LUMPUR
(MICET)
Safety data sheets for ALL chemicals used in your project need to be attached WITH
this form.
Once this assessment has been signed by you, a technician, your project supervisor
and safety health officer; an assigned reference number will be given, please submit:
the original document of this assessment to TECHNICIAN IN-CHARGED [TIC] of

main laboratory used; to be kept in the laboratory safety file.
If more than one laboratory used in this project, submit a copy of this document to
the TIC to be kept in the laboratory safety file.
Ensure this assessment is resubmitted for approval when work out of office hour.
Ensure this assessment is read and signed annually by all concerned.

Ensure this assessment is reviewed annually by the project supervisor.
Remember that anything that you put in this form you must follow!
1.
Title of Procedure / Experiment

Textural and Sensory Evaluation of Traditionally Prepared Meatloaf
with Ohmic Cooked Meatloaf at Various Applied Voltage and Time
2.
Name of author
SHARIFAH SYAHIRAH BINTI
TUAN ABDUL RAHIM
55204212006
Contact Number
0145483059
3.
Name of Academic Supervisor

HISHAMUDDIN BIN JAMALUDIN
4.
Method / Procedure
4.1 Preparation of Meatloaf Emulsion

A mixture of 500 grams of minced beef with minced onions, one whole egg, 70
grams of breadcrumbs, one celery stick, along with approximately 2.5 grams of
salt and black pepper is made. All ingredients measured with analytical balance.
The emulsified ingredients are then placed into a big bowl and covered with an
aluminium foil. It is being refrigerated overnight in order to freeze the meatloaf
emulsion. After 24 hours, the temperature of frozen meatloaf emulsion is reduced
by thawing it in a chiller. The thawed meatloaf emulsion is now ready to be baked
traditionally using baking oven then same formula used to be cooked using ohmic
cooking unit and to be analyzed later on.
4.2 Designing The Meatloaf Ohmic Cooker Unit and Cooking Procedure
The ohmic cooker unit for the meatloaf consists of a transformer, a thermocouple,
multimeter and also an alternating current as its power supply.
Figure 4.1: Electrical Current for Meatloaf Ohmic Cooker Unit
It is made up of a plastic encased in stainless steel whereby the plate that

surrounds the mold from each side is also originated from plastic. The meatloaf
cooker unit is designed with a hole at the back in order to let the melted fats from
the meatloaf to be flown away into a small drainage system at the bottom plate of
the cooker unit. When the meatloaf emulsion sample is being put into the mold, its
initial thickness and length is recorded using a vernier calliper. Then, the mold is
being sandwiched together by the electrode plates. After the life wire and neutral
wire is then connected to the transformer, power supply is turned on and initial
voltage of 40V is set manually by using the multimeter. The voltage capacity is up
to 240V only. At the same time, the initial temperature used is recorded using the
thermocouple by inserting it into a small hole at the front of the mold ensuring it
to have a touch with the meatloaf emulsion. Multimeter A must be set to read the
current until desired voltage is obtained thus start button can be switched on. The
time gap for temperature recording is 10 minutes until a constant or a stable
reading of resistivity is obtained therefore the cooking process can be stopped.
The meatloaf emulsion is taken out from the mold and the length together with the
thickness is measured again using vernier calliper. Next, the cooked meatloaf is
cut to have a look of its internal texture. The same steps are repeated again using
voltages of 50V and 60V. All three cooked meatloaves are being analyzed for its
color using lovibond tintometer, determination of fat, protein and moisture content
as well as tested for its microbial content. The electrical conductivity observed is
recorded.
4.3 Proximate Analysis
4.3.1 Determination of Fat Content
All three samples are prepared in duplicate form. Weight of empty solvent beaker
used was weighed first followed by 2grams (0.01) of pre dried sample prepared
into a pre dried extraction thimble together with filter paper and covered up with a
cotton wool. The thimbles prepared is then being transferred in a soxhlet
extraction machine (Soxhlet Extractor Darmstadt, Germany). Solvent beaker is
added with 125 ml of petroleum ether. After 2 hours and 30 minutes the extraction
should be completed. However, 10 minutes before the extraction is completed,
oven is pre-heated at 100C. Solvent beaker with extracted fat in it is taken out
using towel and moved into the oven to be dried for 30 minutes. Then, it is being
cooled using a dessicator and weighed. The formula used is
Weight of fat = (weight of beaker + weight of fat) weight of beaker
% fat wet basis = (
4.3.2
weight of fat
100(7)
weight of original sample
Determination of Protein Content

All of the samples include the blank sample is prepared in duplicate form and they
should be homogeneous. Three steps are involved in the determination of protein
content which are digestion, distillation and titration. Digestion started with 2
grams of meatloaf sample is put into the digestion tube and filter paper is used to
weight the sample. Empty digestion tube for blank is prepared. Each tube is added
with 2 kjeltabs of Cu 3.5 first and then 12ml of H 2SO4 added afterwards. Tube is
shaken to ensure that the sample is wetted together with the acid. Digestion is
done using the Kjedahl digester. The exhaust system is attached to digestion tube
in rack and the water aspirator is set to full effect. Rack is loaded with exhaust into
a preheated digestion block with temperature of 420C. After that, START button
is pressed and digestion is done continously until all samples are clear with blue or
green solution (approximately 1 hour 15 minutes of time taken). Then, the rack of
tubes is removed with exhaust remained in the tray. Cooling down process is done
for 10 to 20 minutes. Next step is distillation which started with addition of 80ml
of deionized water into each cooled tube carefully. 25ml of 4% boric acid as
receiver solution is added which also containing the indicator methyl blue and
methyl red to a conical flask. They are placed into the distillation unit and
platform is raised to ensure that the distillation outlet is submerged in the receiver
solution. Digestion tubes are connected to the distillation unit and safety door is
closed. Then, analyze button is pressed. 50 ml of 40% NaOH is dispensed into the
tube. Receiver solution in the distillation flask has light green colour indicating
the presence of an alkali-ammonia. Final step is the titration. The distillate with
standardized HCl (approximately 0.1000N or 0.2000N) is titrated until there is
changes of colour in the distillate; from light green to light grey colour. Thus,
volume of acid consumed in the titration method is noted as Vsample.
%N =
[( Normality HCl)(VsampleVblank ) 0.014 100]

Weight of Sample
%Protein=%N Conversion Factor , f

4.3.3
Determination of Moisture Content

Using the oven-drying method, approximately 5grams of the meatloaf sample is
put in to a moisture pan and being placed into the oven. It is left to dry for 21
hours and a half. Next, the moisture pan is taken out from the oven and being
cooled down for 20 minutes before the weight is recorded. The process of heating
and weighing the sample is repeated until successive weight has no difference of
more than one milligram. The formula used to calculate the weight loss is:
% moisture =
weight of wet samplewet of dry sample

100
weight of wet sample
4.4 Determination of CFU/g

4.4.1 Preparation of Food Homogenate
Each 5 grams from uncooked meatloaf sample, cooked meatloaf at 40V, 50V and
60V are weighed. Then, the samples are mixed into a stomacher bag with the
addition of 45ml of peptone water. The food sample is blended in a stomacher at a
speed of 15000 rpm for 2 minutes.
4.4.2
Serial Dilution
1ml of blended stock is pipette into 9ml of diluent that contain peptone water. The
mixture is then being shaken generously to ensure that it is homogeneously mixed.
After that, a subsequent transfer of same volume is done into a second tube
containing a sterile, 9ml diluent from the previous first tube. First tube labelled
with concentration of 10-1 of cells and 10-2 for the second tube. The process of
serial dilution is continued until the concentration of 10-4 is obtained.
Figure 4.2: Schematic Diagram of Serial Dilution

4.4.3
Plating Process
0.1 ml of bacteria from each dilution is pipette onto an agar plate at the center.
Then, L-shaped glass rod or hockey stick rod is steriled by dipping it into a beaker
of ethanol and passed through a flame to burn off the alcohol. The agar surface in
the plate is spreaded evenly using the sterile glass rod. Then, the same sterilization
procedure is done again with the L-shaped glass rod. Procedure is repeated in
order to inoculate the remaining plates. Next, all of the plates are sealed and
labelled according to its number of dilution.
4.4.4
Incubation
Sealed plates are incubated in incubator at temperature of 30C to 35C for 48
hours. After two days, the colonies appeared on the plates are counted.
4.4.5
Colony Counting
The number of bacteria between 30 to 300 is counted and data obtained is
tabulated in table as below:
Number of
Dilution
Dish 1
Dish 2
bacteria per
gram of food
10-1
10-2
10-3
10-4
4.5 Sensory Analysis

The cooked meatloaf is used to be evaluated in a sensory laboratory. 10 panellists
chosen will be served with warm cooked meatloaf. Before the sensory evaluation
started, a short briefing session regarding the cooking method and correct step to
evaluate the sample is done. Sensory characteristic involved in the evaluation
includes the meatloaf colour, tenderness, juiciness, taste and overall acceptance.
Results obtained is presented in ANOVA.
4.6 Flowchart
Preparation of Meatloaf
Emulsion
Designing The Meatloaf

Cooker Unit and Cooking
Procedure
Proximate Analysis
(Determination of Fat, Protein
and Moisture Content)
Determination of CFU/g
Type of hazards identified in this project.
Sensory Analysis
(All hazards must be identified during sampling in & out the campus, performing the experiment in the laboratory and analyzing of results).
Potential Hazards
Type of hazards
Table to be completed
Physical hazards
Please fill in table 5a
Biological hazards
Please fill in table 5a
Chemical hazards
Please fill in table 5b
5a Hazards that have potential to cause harm (potential hazard)

( Do not forget to include ergonomic hazards AND all the physical hazards)
Hazards identified
Precautions / Emergency action
Broken Glassware
Wear glove, safety shoes and jacket
Handling hot equipment or apparatus
Chemical spills
Wear glove, mask, safety shoes and jacket
Electric shock
Exposure to non-ionizing radiation
Wear glove, mask, safety shoes and jacket
Yes
No
5b. Substances used which have potential to cause harm (potential hazard)
NB: This section is concerned with hazards inherent in the substances rather than the way they might be used in this procedure. All columns must be filled
according to Safety Data Sheet.
Substance
Petroleum Ether
Hazards identified
(e.g. toxic, flammable)
Potential Acute Health
Effects:
Hazardous in case of eye
contact (irritant), of
ingestion, of inhalation.
Slightly hazardous in case
of skin contact (irritant,
permeator).
Potential Chronic Health
Effects:
CARCINOGENIC
EFFECTS: Not available.
MUTAGENIC EFFECTS:
Not available.
TERATOGENIC EFFECTS:
Not available.
DEVELOPMENTAL
TOXICITY: Classified
Development toxin
[POSSIBLE]. The
substance may be toxic to
skin, eyes, central
nervous system (CNS).
Repeated or prolonged
exposure to the substance
can produce target organs
damage.
MEL or OEL (if assigned Precautions in handling Emergency action

- do not quote LD50 etc.)
(if gloves required state type)
in the event of spillage etc.
TWA: 300 from ACGIH (TLV)

[United States] [1995] TWA:
1370 from ACGIH (TLV) [United
States] [1995] Consult local
authorities for acceptable
exposure limits.
Precautions:
Keep locked up.. Keep away from
heat. Keep away from sources of
ignition. Ground all equipment
containing material. Do not
breathe gas/fumes/ vapor/spray.
Avoid contact with eyes. Wear
suitable protective clothing. In case
of insufficient ventilation,
Eye Contact:
Check for and remove any
contact lenses. Immediately flush
eyes with running water for at
least 15 minutes, keeping eyelids
open. Get medical attention.
Skin Contact: Wash with soap
and water. Cover the irritated skin
with an emollient. Get medical
attention if irritation develops.
Serious Skin Contact: Not
available.
Inhalation:
If inhaled, remove to fresh air. If
not breathing, give artificial
respiration. If breathing is difficult,
give oxygen. Get medical
attention.
Serious Inhalation:
Evacuate the victim to a safe area
as soon as possible. Loosen tight
clothing such as a collar, tie, belt
or waistband. If
breathing is difficult, administer
oxygen. If the victim is not
breathing, perform mouth-tomouth resuscitation. Seek
medical
attention.
Ingestion:
Do NOT induce vomiting unless
wear suitable respiratory

equipment. If you feel unwell, seek
medical attention and show the
label when possible.
Storage:
Store in a segregated and approved
area. Keep container in a cool, wellventilated area. Keep container
tightly closed and
sealed until ready for use. Avoid all
possible sources of ignition (spark
or flame).
Ethanol (C2H5OH)

Effects:
Hazardous in case of skin
contact (irritant), of eye
ingestion, of inhalation.
of
skin contact (permeator).
Effects:
CARCINOGENIC
EFFECTS: Classified
PROVEN by State of
California Proposition 65
[Ethyl alcohol 200 Proof].
Classified
A4 (Not classifiable for
human or animal.) by
ACGIH [Ethyl alcohol 200
Proof]. Classified A4 (Not
classifiable for humanor
animal.) by ACGIH, 3 (Not
classifiable for human.) by
IARC [Isopropyl alcohol].
MUTAGENIC EFFECTS:
Mutagenic for
mammalian somatic cells.
[Ethyl alcohol 200 Proof].
Mutagenic for bacteria
and/or yeast. [Ethyl alcohol
200 Proof]. Mutagenic
Ethyl alcohol 200 Proof TWA:

1000 (ppm) from ACGIH (TLV)
[United States] [1999] TWA:
1000 (ppm) from OSHA (PEL)
[United States] TWA: 1900
(mg/m3) from OSHA (PEL)
[United States] TWA: 1000
(ppm) from NIOSH TWA: 1000
(ppm)
[United Kingdom (UK)] TWA:
1920 (mg/m3) [United Kingdom
(UK)] TWA: 1000 STEL: 1250
(ppm) [Canada] Isopropyl
alcohol TWA: 983 STEL: 1230
(mg/m3) [Australia] TWA: 200
STEL: 400 (ppm) from ACGIH
(TLV) [United States] [1999]
TWA: 980
STEL: 1225 (mg/m3) from
NIOSH TWA: 400 STEL: 500
(ppm) from NIOSH TWA: 400
STEL: 500 (ppm) [United
Kingdom
(UK)]
Precautions:
Keep locked up.. Keep away from
heat. Keep away from sources of
ignition. Ground all equipment
containing material. Do
not ingest. Do not breathe
gas/fumes/ vapor/spray. Wear
suitable protective clothing. In case
of insufficient ventilation, wear
suitable respiratory equipment. If
ingested, seek medical advice
immediately and show the container
or the label. Avoid
contact with skin and eyes. Keep
away from incompatibles such as
oxidizing agents, acids, alkalis,
moisture.
Storage:
Store in a segregated and approved
area. Keep container in a cool, wellventilated area. Keep container
tightly closed and
sealed until ready for use. Avoid all
possible sources of ignition (spark
or flame). Do not store above 23C
(73.4F).
directed to do so by medical
personnel. Never give anything
by mouth to an unconscious
person. If large quantities of this
material are swallowed, call a
physician immediately. Loosen
tight clothing such as a collar,
tie, belt or waistband.
Serious Ingestion: Not available.
Eye Contact:
open. Cold water may be used.
Get medical attention.
Skin Contact:
In case of contact, immediately
flush skin with plenty of water.
Cover the irritated skin with an
emollient. Remove contaminated
clothing and shoes. Cold water
may be used.Wash clothing
before reuse. Thoroughly clean
shoes before reuse. Get medical
attention.
Serious Skin Contact:
Wash with a disinfectant soap
and cover the contaminated skin
with an anti-bacterial cream. Seek
medical attention.
Inhalation:
attention if symptoms appear.
Serious Inhalation:
for mammalian somatic

cells. [Methyl alcohol].
Mutagenic for bacteria
and/or yeast. [Methyl
alcohol]. TERATOGENIC
EFFECTS: Classified
PROVEN for human [Ethyl
alcohol 200 Proof].
Classified POSSIBLE for
human [Methyl alcohol].
DEVELOPMENTAL
TOXICITY: Classified
Development toxin
[PROVEN] [Ethyl alcohol
200 Proof]. Classified
Reproductive
system/toxin/female,
Reproductive
system/toxin/male
[POSSIBLE] [Ethyl alcohol
200 Proof]. Classified
Reproductive system/
toxin/female, Development
toxin [POSSIBLE]
[Isopropyl alcohol].
Classified Reproductive
Reproductive
system/toxin/male
[POSSIBLE] [Methyl
alcohol]. The substance is
toxic to blood, the
reproductive system, liver,
upper
respiratory tract, skin, eyes,
central nervous system
(CNS). The substance may
be toxic to kidneys, lungs,
brain, peripheral nervous
system, gastrointestinal
tract. Repeated or
or waistband. If
breathing, perform mouth-tomouth resuscitation. Seek
medical
attention.
Ingestion:
person. Loosen tight clothing
such as a collar, tie, belt or
waistband. Get medical attention
if symptoms appear.
Sodium Hydroxide (NaOH)
prolonged exposure to the

substance can produce
target organs damage.
Effects:
Very hazardous in case of
skin contact (corrosive,
irritant, permeator), of eye
contact (irritant, corrosive),
of ingestion,
of inhalation. The amount
of tissue damage depends
on length of contact. Eye
contact can result in
corneal damage or
blindness. Skin contact can
produce inflammation and
blistering. Inhalation of dust
will produce irritation to
gastro-intestinal or
respiratory tract,
characterized by burning,
sneezing and coughing.
Severe over-exposure can
produce lung damage,
choking,
unconsciousness or death.
Inflammation of the eye is
characterized by redness,
watering, and itching. Skin
inflammation is
characterized by itching,
scaling, reddening, or,
occasionally, blistering.
Effects:
CARCINOGENIC
MUTAGENIC EFFECTS:
Mutagenic for mammalian
somatic cells.
STEL: 2 (mg/m3) from ACGIH

(TLV) [United States] TWA: 2
CEIL: 2 (mg/m3) from OSHA
(PEL) [United States] CEIL: 2
(mg/
m3) from NIOSHConsult local
authorities for acceptable
exposure limits.
Precautions:
Keep container dry. Do not breathe
dust. Never add water to this
product. In case of insufficient
ventilation, wear suitable
respiratory equipment. If you feel
unwell, seek medical attention and
show the label when possible. Avoid
contact with skin
and eyes. Keep away from
incompatibles such as oxidizing
agents, reducing agents, metals,
acids, alkalis, moisture.
Storage: Keep container tightly
closed. Keep container in a cool,
well-ventilated area. Hygroscopic.
Deliquescent.
Eye Contact:
contact lenses. In case of contact,
immediately flush eyes with
plenty of water for at least 15
minutes. Cold water may be
used. Get medical attention
immediately.
Skin Contact:
flush skin with plenty of water for
at least 15 minutes while
removing contaminated clothing
and shoes. Cover the irritated
skin with an emollient. Cold water
attention immediately.
medical attention.
Inhalation:
Serious Inhalation:
or waistband. If
breathing, perform mouth-to-
Not available.
DEVELOPMENTAL
TOXICITY: Not available.
The substance may be
toxic to
mucous membranes, upper
respiratory tract, skin, eyes.
can produce target
organs damage. Repeated
exposure of the eyes to a
low level of dust can
produce eye irritation.
Repeated skin exposure
can
produce local skin
destruction, or dermatitis.
Repeated inhalation of dust
can produce varying
degree of respiratory
irritation or
mouth resuscitation. WARNING:

It may
be hazardous to the person
providing aid to give mouth-tomouth resuscitation when the
inhaled material is toxic,
infectious or
corrosive. Seek immediate
medical attention.
Ingestion:
person. If large quantities of this
material are swallowed, call a
physician immediately. Loosen
tight clothing such as a collar,
tie, belt or waistband.
lung damage.
Hydrochloric Acid (HCl)

Effects:
of ingestion, . Slightly
hazardous in case of
inhalation (lung sensitizer).
Non-corrosive for lungs.
Liquid or spray mist may
produce tissue damage
particularly on mucous
membranes of eyes, mouth
and respiratory tract. Skin
contact may produce burns.
CEIL: 5 (ppm) from OSHA (PEL)

[United States] CEIL: 7 (mg/m3)
from OSHA (PEL) [United
States] CEIL: 5 from NIOSH
CEIL: 7 (mg/m3) from NIOSH
TWA: 1 STEL: 5 (ppm) [United
Kingdom (UK)] TWA: 2 STEL: 8
(mg/m3) [United Kingdom
(UK)]Consult local authorities for
acceptable exposure limits.
Precautions:
Keep locked up.. Keep container
dry. Do not ingest. Do not breathe
gas/fumes/ vapor/spray. Never add
water to this product.
In case of insufficient ventilation,
equipment. If ingested, seek
medical advice immediately and
show
the container or the label. Avoid
oxidizing agents, organic
materials, metals, alkalis, moisture.
May corrode metallic surfaces.
Eye Contact:
immediately.
Skin Contact:
Inhalation of the
spray mist may produce
severe irritation of
respiratory tract,
characterized by coughing,
choking, or shortness of
breath.
Severe over-exposure can
result in death.
Inflammation of the eye is
characterized by redness,
watering, and itching. Skin
inflammation is
scaling, reddening, or,
occasionally, blistering.
Effects:
of skin contact (sensitizer).
CARCINOGENIC
EFFECTS: Classified 3
(Not classifiable for
human.) by IARC
[Hydrochloric acid].
MUTAGENIC EFFECTS:
Not available.
Not available.
DEVELOPMENTAL
toxic to kidneys, liver,
mucous membranes, upper
respiratory tract, skin, eyes,
Circulatory System, teeth.
damage. Repeated or
prolonged contact with
Store in a metallic or coated

fiberboard drum using a strong
polyethylene inner package.
well-ventilated area.

immediate medical
attention.
Inhalation:
Serious Inhalation:
or waistband. If
breathing, perform mouth-tomouth resuscitation. WARNING:
It may
infectious or
medical attention.
Ingestion:
If swallowed, do not induce
vomiting unless directed to do so
by medical personnel. Never give
anything by mouth to an
unconscious person. Loosen tight
or waistband. Get medical

chronic eye irritation and
severe skin irritation.
exposure to spray mist may
produce respiratory tract
irritation leading to frequent
attacks of bronchial
infection. Repeated
exposure to a highly toxic
material may produce
general deterioration of
health by an accumulation
in one
or many human organs.
Boric Acid with indicator

Effects:
Extremely hazardous in
case of ingestion.
contact (irritant). Slightly
hazardous in case of skin
inhalation.
Effects:
Extremely hazardous in
case of ingestion.
contact (irritant).
CARCINOGENIC
EFFECTS: Not
available. MUTAGENIC
Not available.
DEVELOPMENTAL
TOXICITY:
Classified Reproductive
Ethyl alcohol 200 Proof TWA:

1000 from OSHA (PEL) [United
States] TWA: 1900 from OSHA
(PEL) [United States] Consult
local authorities for acceptable
exposure limits.
Precautions:
Keep locked up.. Do not ingest. Do
not breathe gas/fumes/ vapor/spray.
Avoid contact with eyes. Wear
suitable protective
clothing. If ingested, seek medical
advice immediately and show the
container or the label.
well-ventilated area.
Eye Contact:
open. Cold water may be used.
Get medical attention.
Skin Contact:
Wash with soap and water. Cover
the irritated skin with an emollient.
Get medical attention if irritation
develops. Cold water
may be used.
Serious Skin Contact: Not
available.
Inhalation:
attention.
Serious Inhalation: Not
available.
Ingestion:
Sulfuric Acid (H2SO4)
Reproductive
system/toxin/male
[PROVEN] [Ethyl alcohol
200 Proof]. The substance
is toxic to lungs, the
reproductive system,
mucous membranes.
damage.
Effects:
of ingestion,
of inhalation. Liquid or
tissue damage particularly
on mucous membranes of
eyes, mouth and
respiratory tract. Skin
contact may produce burns.
Inhalation of the spray mist
may produce severe
irritation of respiratory
tract, characterized by
coughing, choking, or
shortness of breath. Severe
over-exposure can result in
death. Inflammation of
the eye is characterized by
redness, watering, and
itching. Skin inflammation is
scaling, reddening,
or, occasionally, blistering.
Effects:
if symptoms appear.
TWA: 1 STEL: 3 (mg/m3)
[Australia] Inhalation TWA: 1
(mg/m3) from OSHA (PEL)
[United States] Inhalation TWA:
1 STEL: 3
(mg/m3) from ACGIH (TLV)
[United States] [1999] Inhalation
TWA: 1 (mg/m3) from NIOSH
[United States] Inhalation TWA:
1
(mg/m3) [United Kingdom
(UK)]Consult local authorities for
acceptable exposure limits.
Precautions:
Keep locked up.. Keep container
dry. Do not ingest. Do not breathe
gas/fumes/ vapor/spray. Never add
water to this product.
In case of insufficient ventilation,
equipment. If ingested, seek
medical advice immediately and
show
the container or the label. Avoid
oxidizing agents, reducing
agents, combustible materials,
organic materials, metals, acids,
alkalis, moisture. May corrode
metallic surfaces. Store in a
metallic or coated fiberboard drum
using a strong polyethylene inner
package.
Storage:
Hygroscopic. Reacts. violently with
water. Keep container tightly
well-ventilated area. Do not
store above 23C (73.4F).
Eye Contact:
immediately.
Skin Contact:
immediate medical
attention.
Inhalation:
CARCINOGENIC
EFFECTS: Classified 1
(Proven for human.) by
IARC, + (Proven.) by
OSHA. Classified A2
(Suspected for
human.) by ACGIH.
MUTAGENIC EFFECTS:
Not available.
Not available.
DEVELOPMENTAL
toxic to kidneys, lungs,
heart, cardiovascular
system, upper respiratory
tract,
eyes, teeth. Repeated or
prolonged exposure to the
substance can produce
target organs damage.
contact with spray mist may
produce chronic eye
irritation and severe skin
irritation. Repeated or
prolonged exposure to
spray
mist may produce
respiratory tract irritation
leading to frequent attacks
of bronchial infection.
Repeated exposure to a
highly
toxic material may produce
general deterioration of
health by an accumulation
in one or many human
organs.
Serious Inhalation:
or waistband. If
breathing, perform mouth-tomouth resuscitation. WARNING:
It may
infectious or
medical attention.
Ingestion:
if symptoms appear.
Skin Contact Flush with copious amounts of water for at least 15 minutes. Remove contaminated clothing and wash before reuse. Unless contact has been
slight obtain medical attention.
Inhalation Remove to fresh air, rest and keep warm. If breathing is difficult give artificial respiration and obtain medical attention.
Eye contact Irrigate with copious amounts of eye wash or water for at least 15 minutes. Assure adequate flushing by separating the eyelids with fingers.
Obtain medical attention
Ingestion Do not induce vomiting. If conscious provide water for person to thoroughly wash out mouth (and sip if required). Obtain medical attention.
Full safety data sheets for the above chemicals must available in file at the laboratory in use.
Please ensure data sheets for any previously unused chemical are added to the laboratory's safety file.
Nomenclature
CoSHH: Control of Substances Hazardous to Health
MSDS : Material Safety Data Sheet
MEL:
OEL:
Maximum exposure limit

Occupational exposure limit
TWA:
Time weighted average
5c.
Equipment used in the procedure which may cause harm
NB: This section is concerned with hazards inherent in the equipment rather than the way it might
be used in this procedure.
Equipment
Nature of
hazard
Precautions to be taken
Soxhlet Extractor
Electrical Hazard
Chemical Hazard
Stomacher
Chiller
Electrical Hazard
Biological Hazard
Electrical Hazard
Chemical Hazard
Refrigerator
Electrical Hazard
Chemical Hazard
Meatloaf Ohmic Cooker Unit
Glassware
Electrical Hazard
Physical Hazard
Biological Hazard
Ensure the electrical connection is

correct
Wear proper PPE and gloves
Excessive drying may oxidize the fat
and give high results.
After extraction use steam bath or water
bath to evaporate petroleum ether. Allow
evaporation to continue several minutes
beyond the point where no ether is
observed in order to minimize personal
exposure.
When working with petroleum ether,
work in a hood in well ventilated area.
Keep away from sources of ignition.
correct
correct
Ensure that chemicals and food are not
stored in same equipment.
correct
Ensure that chemicals and food are not
stored in same refrigerator. Each of them
need separate refrigerator.
correct
Repaired glassware is subject to thermal
shock and should be avoided. Thinwalled, Erlenmeyer or round-bottomed
flasks larger than 1 L should never be
evacuated.
Ensure that glasswares are wellsterilized before using to avoid
microbial contamination
It is advisable to check for flaws such as
star cracks or scratches before using any
glassware
Drying Oven & Baking Oven
Electrical Hazard
Physical Hazard
Colony Counter
Electrical Hazard
Dessicator
Physical Hazard
Bunsen Burner
Physical Hazard
Incubator
Electrical Hazard
Equipment should not be used to dry

any chemical sample that might pose a
hazard because of acute or chronic
toxicity unless special precautions have
been taken to ensure continuous venting
of the atmosphere inside the oven.
During a thunderstorm, turn off the
power key immediately, then turn off the
earth leakage breaker and the main
power. If this procedure is not followed,
fire or electrical shock may be caused.
During and immediately after operation,
the internal surfaces of the chamber and
the door are extremely HOT." To
prevent injury, take out the samples
when the chamber has cooled down or
wear gloves while the chamber is still
hot.
Do not touch the internal surfaces of the
chamber and the door when you will
open the door, because they are
extremely hot.
correct
Ensure the surrounding area is clean and
dry
correct
Maintain an airtight seal between the lid
and body of dessicator
Once the desiccant has been added, the
lid to the desiccator should not be
removed any more than is necessary.
An ignited crucible or other very hot
object should be cooled for about one
minute before being placed into the
desiccator to avoid partial vacuum
formation in order to ease the process of
removing the lid without destroying the
sample inside.
Ensure that the host is fit securely
Ensure to have a little distance when
lighting up the bunsen burner
Do not touch the hot surface
Handle with care
Ensure that incubator is in good
condition
Lovibond Tintometer
Electrical Hazard
Do not place solution on top of the

Lovibond Tintometer casing.
The Lovibond Tintometer cells should

never overfilled.
To avoid the risk of permanent eye
damage, never open the lamp housing
when the light sources are illuminated.
When finished with then Lovibond
Tintometer, the cell holder should be
removed and washed with distilled
water.
Biological Hazard
Analytical Balance
Electrical Hazard
Electrical Hazard
Kjeldahl Digester
Chemical Hazard
Protective weighing chamber must be

free of dust at all times
When not in use, the door of the
chamber must be kept closed.
The weighing pan or the weighing
platform should be cleaned regularly
and they should not be touched without
using gloves as this keeps grime and
fingerprints away from the balance.
Use a soft cloth and a paintbrush to
clean the analytical balance.
Be certain that the arresting mechanism
of the beam is engaged whenever the
loading on the balance is being changed
and when the balance is not in use.
Do not attempt to adjust or repair the
balance if you are not trained to do so.
Do not weight an object that has been
heated until it has returned to the room
temperature.
Always place the balance on a vibration
free platform.
Use proper personal protective
equipment.
correct If any spills or leaks occur, clean
up spills immediately. Vacuum or sweep
up material and place into a suitable
disposal container. Avoid generating
dusty conditions and provide proper
ventilation.
NOTE:
ALL EQUIPMENTS USED ELECTRICAL POWER WILL HAVE ELECTRICAL HAZARDS.
5d. Laboratories required

1
2
3
Food Analysis Laboratory

Food Processing Laboratory
Food Sensory Laboratory
Food Microbiology Laboratory
5e. First Aid & Fire Fighting Equipment (FAFFE) Available

FAFFE
Eye wash & emergency
shower
Antiseptic cream
Dressing
Eyemo
Yellow lotion
Dettol
Chemical spill kit
Fire Extinguisher
LABORATORY:
Processing
Expire: 04/2016
Triangular Bandage
Pin
Scissor
Gauze Bandage
Cotton Bud
Minyak Cap Kapak
Expire: 02/2016
Expire: 08/2017
Expire: 05/2016
Checked on: Next checking on: -
FAFFE
LABORATORY: Sensory
Eye wash & emergency

shower
Antiseptic cream
Dressing
Eyemo
Yellow lotion
Dettol
Chemical spill kit
Fire Extinguisher
Expire: 04/2016
Scissor
Absorbent Cotton Wool
Gauze Swabs
Elastic Bandage
Cotton Wool
Minyak Cap Kapak
Upore
Expire: 02/2016
Expire: 08/2017
Expire: 05/2016
Checked on: 23/09/2014
Next checking on:
24/02/2015
LABORATORY: Analysis
Model: 8300 (HAWS
Manufacturing)
Last date checked:10/13
Condition: GOOD
Expire: 04/2016
Triangular Bandage
Pin
Scissor
Gauze Bandage
Cotton Bud
Minyak Cap Kapak
Expire: 02/2016
Expire: 08/2017
Expire: 05/2016
Checked on:11/09/2012
Next checking on:
10/09/2013
LABORATORY:
Microbiology
Model: 8300 (HAWS
Manufacturing)
Last date checked: 22/8
Condition: NOT GOOD
Expire: 04/2016
Triangular Bandage
Pin
Scissor
Gauze Bandage
Cotton Bud
Minyak Cap Kapak
Expire: 02/2016
Expire: 08/2017
Expire: 05/2016
Checked on: 11/09/2012
Next checking on:
10/09/2013
6.
Disposal routes for waste materials
Tick [ ] or complete appropriate box for each category of waste WITH reference to FIRST
SCHEDULE of ENVIRONMENTAL QUALITY (SCHEDULED WASTES) REGULATIONS 2005.
Nature of
waste:
Deionized Water
Sink
Black
bag
Route for disposal:

Black Solvent drum Sharps
(SW311;
bin (e.g.
bin
SW322;
SW323)
SW410)
Other route
(specify)
Filter Paper
Peptone Water
Boric Acid with
indicator
SW322
Ethanol
Sulfuric Acid
Hydrochloric Acid
Sodium Hydroxide
SW322
Petroleum Ether
Broken Glassware
Gloves, Mask and
Tissues
NOTE:The following list of scheduled wastes are not exhausted, the above regulation must be
referred to complete this section.
SW109
SW206
SW311
SW322
SW323
SW421
Waste containing mercury or its compound

Spent inorganic acids
Waste of oil or oily sludge
Waste of non-halogenated organic solvents
Waste of halogenated organic solvents
Rags, plastics, papers or filters contaminated with scheduled wastes
NOTE:
PLEASE SPECIFY YOUR BIOHAZARD WASTE - ROUTE OF DISPOSAL
7a. Specific risk assessment for procedure and precautions to be

taken
Before any experiment is conducted, one should be prepared in terms of every hazard available and
must be familiar enough with the procedures of handling each equipments to be used in order to
avoid any unwanted incidents happened. If all precautionary steps as stated in Table 5a and Table 5b
are followed in details, there would be low risk of contact with hazardous chemicals and any hazard
can be overcomed.
Every work done in laboratory must be conducted with complete PPE being worn, any work with
hazardous chemicals must be done carefully and in well-ventilated area to avoid chemical spillage
that may lead to bad incidents.
Every electrical equipment must only be switched on after every components have been connected
and the surrounding is not in wet condition. All of the cables should be checked thoroughly to
ensure correct connection of wires and to detect any damages.
Disposal of hazardous chemical must be done according to Table 6 and any glassware used must be
handled with care.
8.
Level of supervision required and suitability for out-of-hours work
Please mark box [ ] to indicate appropriate supervision category for this procedure.
Category A
Category B
Category C
Category D
Category E
work may not be carried out except under the direct supervision of a
specified member of staff who is present continuously
work may not be started without the supervisor's advice and approval, some
additional training and initial direct supervision
work may not be started without the supervisor's advice and approval and
some additional training - no direct supervision required
although extra care must be observed, workers should already be competent
and adequately-trained for this task
risks are insignificant and supervision unnecessary
8a Is this procedure authorised for out-of-hours work ?

YES :___
NO:____
[TO BE FILLED FOR WORK TO BE DONE OUT OF OFFICE HOURS ]
Date and time
Supervisor Approval
HOS Approval
SHO Approval
8b Emergency Contact
No
NAME
CONTACT NO
1.
Security Guard
06-5512054
2.
Safety and Health Officer
019-2436965
Name: NOR AINI BTE BUROK

3.
Supervisor
013-3596003
Name: HISHAMUDDIN BIN

JAMALUDIN
4.
Co-supervisor (if any)

Name:
5.
Note:
Supervisor is required to make hourly phone calls to check on students when they work out of office
hour.
Assessment reference number 9.
Signature of technician involved in the project.
Form Checked By
(Technician):Form Checked By
(Technician):9a.
Signature
(Date)
Signature
(Date)
Signature
(Date)
Signature
(Date)
Signature of academic supervisor and safety& health officer
Note that this assessment needs to be reviewed and signed annually to take into account any
developments of the procedure [if the project is more than one year, eg postgraduate].
Supervisor (Name): Hishamuddin Bin Jamaluddin

Co-Supervisor (Name):
Signature
(Date)
Signature
(Date)
9b. Signature of safety & health officer or Safety and Health

Committee member
Safety & Health Officer /SHEC member
(Name)
Signature
(Date)
10. Signature and contact number of workers involved with this

procedure
Please sign annually to say you have read the attached document and understood it.
Personnel
involved
Status
1
3
5
7
9
11
Assessment reference number
Signature &
Contact No.
(Date)
2
4
6
8
10
12
Personnel
involved
Status
Review Section [TO BE FILLED BY POSTGRADUATE ONLY].

11.
Signature of the reviewer/s
Note that this assessment needs to be reviewed and signed ANNUALLY to take into account any
developments of the procedure
(This section should be signed by the technician/s in charge of the areas where the work is to be
done)
Signature
(Date)
Could any less

hazardous substance or
procedure be used?
Signature
(Date)
Could
hazar
proce

JSA Ira

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Reference Number-

JOB SAFETY / HAZARD ANALYSIS

the original document of this assessment to TECHNICIAN IN-CHARGED [TIC] of

Ensure this assessment is read and signed annually by all concerned.

Title of Procedure / Experiment

Name of Academic Supervisor

4.1 Preparation of Meatloaf Emulsion

Figure 4.1: Electrical Current for Meatloaf Ohmic Cooker Unit

It is made up of a plastic encased in stainless steel whereby the plate that

Determination of Protein Content

[( Normality HCl)(VsampleVblank ) 0.014 100]

%Protein=%N Conversion Factor , f

Determination of Moisture Content

weight of wet samplewet of dry sample

4.4 Determination of CFU/g

Figure 4.2: Schematic Diagram of Serial Dilution

4.5 Sensory Analysis

Designing The Meatloaf

Type of hazards identified in this project.

Please fill in table 5a

Please fill in table 5a

Please fill in table 5b

5a Hazards that have potential to cause harm (potential hazard)

Precautions / Emergency action

Wear glove, safety shoes and jacket

Handling hot equipment or apparatus

Wear glove, safety shoes and jacket

Wear glove, mask, safety shoes and jacket

Wear glove, safety shoes and jacket

Exposure to non-ionizing radiation

Wear glove, mask, safety shoes and jacket

MEL or OEL (if assigned Precautions in handling Emergency action

(if gloves required state type)

in the event of spillage etc.

TWA: 300 from ACGIH (TLV)

wear suitable respiratory

Potential Acute Health

Ethyl alcohol 200 Proof TWA:

for mammalian somatic

Sodium Hydroxide (NaOH)

prolonged exposure to the

STEL: 2 (mg/m3) from ACGIH

mouth resuscitation. WARNING:

Hydrochloric Acid (HCl)

Potential Acute Health

CEIL: 5 (ppm) from OSHA (PEL)

Store in a metallic or coated

before reuse. Thoroughly clean

spray mist may produce

Serious Ingestion: Not available.

or many human organs.

Boric Acid with indicator

Potential Acute Health

Ethyl alcohol 200 Proof TWA:

Sulfuric Acid (H2SO4)

Maximum exposure limit

Time weighted average

Equipment used in the procedure which may cause harm

Meatloaf Ohmic Cooker Unit

Ensure the electrical connection is

Drying Oven & Baking Oven

Equipment should not be used to dry

Do not place solution on top of the