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International Journal of Pharmacognosy and Phytochemical Research 2014-15; 6(4); 850-855
ISSN: 0975-4873
Research Article
RESULTS
Pluchea leaves used to get pluchea leaves extract had
moisture content around 14.19 0.17%. This moisture
content of pluchea leaves is the same as Widyawati et al.
(2011) reported around 14.29%. The yield obtained from
solvent extraction with aquadest, ethanol, methanol, ethyl
acetate, and hexanes was showed at Table 1. Data showed
that aquadest extract had the highest yield (40,65 1,47%).
And then methanolic, ethyl acetate, ethanolic and hexanes
extracts had yield 38.07 2.08, 32.97 1.24, 31.09 1.75,
dan 29.46 1.47 %, respectively. The yield of pluchea
851
Page
1400.0
1200.0
1000.0
800.0
600.0
400.0
200.0
0.0
1185.26.4
369.429.9
174.75.7
Aquadest
Methanol
Ethanol
15.21.3
1.00.0
Ethyl Acetate
Hexanes
7.80.3
0.90.3
Ethyl Acetate
Hexanes
10.30.3
0.20.0
Ethy Acetate
Hexanes
Extract Type
911.965.4
93.12.1
81.31.2
Aquadest
Methanol
Ethanol
Extract Type
1000.0
794.934.3
800.0
600.0
400.0
244.825.2
208.51.2
200.0
0.0
Aquadest
Methanol
Ethanol
Extract Type
2.060.11
2.140.05
1.890.15
2.00
1.380.14
1.50
1.00
0.50
0.020.00
0.00
Aquadest
Methanol
Ethanol
Ethyl acetate
Hexanes
Page
852
Extract Type
Page
853
3.
CONCLUSION
The results obtained demonstrated that methanol was the
most effective solvent to extract phytochemical
compounds compared to aquadest, ethanol, ethyl acetate,
and hexanes. Phytochemical compounds identified
included sterol, flavonoid, saponin, tannin, phenolic,
alkaloid and glycoside compounds. Methanolic extract
obtained had the highest total phenolic content and
antioxidant activity (DPPH scavenging activity and
reducing power).
10.
ACKNOWLEDGEMENT
The authors would like to thank the Higher Education
Directorate (DIKTI) of the Indonesians Government for
the financial support (The competitive research grant) and
Widya Mandala Catholic University in Surabaya for this
research.
13.
REFERENCES
1. Aberoumand A, Deokule SS. Comparison of phenolic
compounds of some edible plants of Iran and India.
Pakistan Journal of Nutrition 2008; 7 (4) : 582-585.
2. Amic D, Davidovic-Amic D, Beslo D, Trinajsti N.
Structure-radical scavenging activity relationships of
flavonoids. Croatica Chemica Acta 2003; 76(1) : 5561.
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