MARIEL O.

PADERNA
MAIDEELYN ANILLO
BRIAN ANGELO R. SUSTRINA
NELO G. PAIRAT
RAYMOND JOHNRICK M. DE JESUS
MARCO FELIX S. VALDEZ
CRSC 2- PRACTICES IN CROP PRODUCTION
BS AGRICULTURE 2-2
September 29, 2016

INTRODUCTION
Lactic acid bacteria (LAB) are widespread microorganisms which can be found in
any environment rich mainly in carbohydrates, LAB are anaerobic microorganisms that
decompose sugar in the absence of oxygen. Normally, they are separated and cultured
with rice washed water and milk. This is how Lactic Acid Bacteria Serum (LABS) is
obtained.
Lactic Acid Bacteria Serum is now used for its ability to convert waste into organic
matter and basic materials. And they thrive and feed on the ammonia released in the
decomposition normally associated with the foul odor (removes foul odor). Lastly, they
serves as defense against pathogenic diseases such as harmful viruses and fungi.
Today LABS application can be observe on field of natural farming, livestock
production, aquaculture, and even in households.

OBJECTIVES
1. To produce homemade LABS using inexpensive basic materials.
2. To identify its different use and applications.
3. To make recommendations to improve the production and to maximize its
effectivity.

METHODOLOGY
MATERIALS TO BE USED:

BOUGHT IN MARKET:
1kg Rice

37.00

1 Kg of Molasses

9.00

OTHERS:
Water
Jar or Container
Plastic Basin

1 kg of Powdered Milk

50.00

Strainer (or Clean cloth)

Weigh Scale (or other measuring types)

TOTAL Cost of Production

= 96.00

Funnel
Paper and Rubber band

PROCEDURE W/ PICTURE
STEP 1: Put 700 ml of water to 1 kg rice then by use of hand stir, swirl and crush the
mixture until milky colored water is attained. (The water is now a rich source of
carbohydrates) Obtain rice wash by filtering the mixture using clean cloth.

Figure 1 Adding 700 ml water to 1 kg of rice

Figure 2 Mixing water and rice

Figure 3 Filtering the mixture

STEP 2: Place 500 ml rice wash to a jar and cover it with paper (use rubber band to tie
the paper covering). Leave the jar in cool dark place for 5-7 days.

Figure 4 Covering the jar with paper

STEP 3: After a week, when the bran has risen and it smells a little sour and forms 3
layers. This is indicating the rice wash is infected with various microbes. Take about 100
ml of clear water by straining again the mixture.

STEP 4: Add 1 L of milk (to obtain 1:10 Water and Milk ratio). You may use skim or
powdered milk, although fresh milk is the best. Cover the jar with paper same as before.
Leave for 5-7 days in cool dark place.

STEP 5: After about 1 week youll see curds (made of carbohydrate, protein, and fat) or
the white part and yellow liquid or whey, enriched with lactic acid bacteria from the
fermentation of the milk. Extract the whey by pouring through a strainer

Figure 5 The yellow liquid is the whey

Figure 6 Adding molasses

Figure 7 Finished Product

STEP 6: Add Molasses or brown sugar in equal part to the collected whey. This is to
preserve the LABS at room temperature. So, if you have 1L of serum, add 1kilo sugar or
1L molasses. Otherwise store in fridge to keep. Then place it in clean container and
label it accordingly

USES AND APPLICATION OF LABS

Based on our research we summarizes the different uses of LABS:
It can be used as alternative strategy to antibiotics growth promoters used in farm
animals. Because continuous use of antibiotics may increase bacterial resistance, which
can threaten the health of both animals and humans. Mix 2tbsp to 1L water, then add that
mixture to animals water at 2tbsp/L (so the animals water contains little less than a
quarter tsp/L of lacto serum). But this is very flexible. The Lacto serum is not harmful, so
its just about adding enough to be effective, without wasting it.

In plants, spraying diluted solution of lactic acid bacteria serum (2tbsp. /1 liter of
water) to the plant and soil helps plant growth and makes them healthier. As it is applied
to the soil or the leaves, these beneficial bacteria aid in the decomposition process, thus
allowing more food to be available and assimilated by the plant. And it is also known to
produce enzymes and natural antibiotics aiding effective digestion and has antibacterial
properties that serves as defense against fungi and viruses.
In Aquaculture, with regular addition of this beneficial organism to poor quality
water (because of high amount of ammonia that pollutes water) ammonia problem is
minimized and helps hasten or complete the denitrification or converting wastes into
forms not harmful to fish. Add lacto at roughly 1L per 700m3 of fish-containing water.
Other applications: Used for its odor killing ability in animal wastes, clogged drains,
foul odor of canals and addition to beneficial organism in composts.
RECCOMENDATION
We recommend that:
1. Use LABS in morning, to avoid exposure to direct sunlight with UV that may
kill or reduce their effectivity.
2. Avoid store milk that have antibiotic components.
3. During production and storage keep away the container from rainwater for
this may cause contamination. Also keep it safe from wild animals.
4. Do not use plastic lids or covering that will prevent aeration. Thats why we
use paper.

5. Use gloves and protective gears, aside from LAB it is possible that it contains
harmfull organism that may enter your body through exposed body parts (ex.
wounds)
CONCLUSION
Lactic acid bacteria serum are therefore excellent material that can be made only
indoors using inexpensive common materials. Modern agriculture must been using this
because as what has been stated it can be substituted for antibiotics for animal growers
and beneficial for plants that may increase income of farmers using it and other useful
applications.

REFERENCES
Ikeda, David M. et al. Sustainable Agriculture, Farming: Lactic Acid Bacteria.
August 2013, CTHR. Minoa, Hawaii
Gilliland, S. E., 1990. Health and nutritional benefits from lactic acid bacteria. FEMS
Microbiol Rev. 1990 Sep;7(1-2):175-88.
Lim, A. K., 2005. Handout on natural farming system and technology seminar. Davao:
Tribal Mission Foundation International, Inc.
Guolong Zhang, The Use of Lactic Acid Bacteria as a Probiotic in Swine Diets, Mar.
2015 URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4384071/
Rory Turnbull, DIY Lacto Bacillus Culture, Jan. 2013 URL:
https://permies.com/t/25812/permaculture/DIY-Lacto-Bacillus-Culture
Gil and Patrick, 2016 URL:
http://theunconventionalfarmer.com/recipes/lactobacillus-serum/
Dugas, J., n.d. Lactic acid bacteria. URL:
http://www.waksmanfoundation.org/labs/mbl/lactic.html.