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IS 548-2-20 (1983): Methods of Sampling and Tests for Oils

and Fats, Part 2: Purity Tests, Section 20: Test for
Detection of TARAMIRA Oil in Mustard/Rapeseed Oil [FAD 13:
Oils and Oilseeds]

! $ ' +-
Satyanarayan Gangaram Pitroda

Invent a New India Using Knowledge

! > 0 B

BharthariNtiatakam

Knowledge is such a treasure which cannot be stolen

IS : 548 ( Part P/Set 20 ) - 1983

(Reaffirmed xxxxx
2000)

Indian Standard

2010

METHODS OF
SAMPLING AND TESTS FOR OILS AND FATS
PART
Section 20

PURITY

Test for Detection of

TESTS

TARAMIRA

Oil in Mustard/

Rapeseed Oil
( First

Reprint FEBRUARY 1993 )

665.3.34.83+665.334.94:543.869

UDC

@ Copyright 1983
BUREAU
MANAK

OF

BHAVAN,

INDIAN
9

BAHADUR

STANDARDS
SHAH

ZAFAR

MARG

NEW DELHI 1 lOW2

Gr2

.Novcmbzr 3983

IS : 548 (Part

P/Set 20 ) - 1983

Indian Standard
METHODS OF
SAMPLING AND TESTS FOR OILS AND FATS
PART

Section

20

PURITY

Test for Detection of

TESTS

TARAMIRA

Oil in Mustard/

Rapeseed Oil

Oils and Oilseeds Sectional Committee, CAFDC

RepresentiNg

Chairman
PROB M. M. CRAKRABARTY

Oil Technologists

Association of India, Kanpur

Members
PROF A, C. GUPTA ( Alternate to
Prof M. M. Chakrabarty )
Central Organization
for Oil Industry and Trade,
SHRI S. N. A~ARWAL
Bombay
SHRI H. P. GUPTA ( Alternate )
MARI~ETIN~ Directorate of Marketing and Inspection, Nagpur
AORICULTURAL
ADW~ER TO THE GOVERNMENT
OF INDIA
SHRI R. J. VERMA ( Alternate )
Ministry of Defence ( DC1 )
SHRI J. A. A~HTAPUTRE
SHRI P. N. A~ARWAL ( Alternate )
Tata Oil Mills Co Ltd, Bombay
DR B. P. BALIQA
DR N. L. MURTY ( Aftcrwte )
Swastik Household and Industrial Products Ltd,
SHHI M. A. BI~ATT
Bombay
SRRI C. R. KRISHNAMURTHY ( Alternate )
Hindustan Lever Ltd, Bombay
DR N. V. BRIXQI
DR V. V. S. MANI ( Alternate )
Central Committee for Food Standards ( Ministry
SHRI D. S. CHADHA
of Health and Family Welfare ), New Delhi
SHRIMATI DEBI MUKHERJEE ( Alternate )
National Test House, Calcutta
.%RI P. K. CHA~RABORTY
Vanaspati
Manufacturers
Association of India,
DR A. C. CHATRAPATI
Bombay
SHRI RAJ KAPILA ( Alternate)
Central Food Laboratory, Calcutta
DR K. C. GUHA
SHR-~.4. K. .D,H~x ( Af&,lale )
Kusum Products Ltd, Rishra
SHRI S. P. GIPT \
SlrRT A. R. N. KI;LU ( Afternate )
Godrej Soaps Ltd, Bombay
SHRI B. V. KANT \R
SHRI M. S. TH.\~;uR ( Alternate)
7

( Continued on p lee 2 )
$9 Copyr:,qhf 1983
BUREAU
OF INDIAN
STANDARDS
This publicalion IS protected under the I&ON Copyright Act ( XIV
of 1957 ) and
reproduction
in whole or in part by any means except with written permission of the
publisher shall br deemed to be ao infringement of copyright under the said Act.

IS t !i48 ( Part
( fhzlind

from

2jscc 20 ) - 1983

page 1 )

Wmbsrs

Rrpesenting
Directorate of Vanaspati,
Vegetable
Oils and Rat
( Ministry of Civil Supplies ), New Delhi
Regional Research Laboratory
( CSIR ), Hyderabad

DH M. K. KVNDU

DE G. LAKSHYINARAYANA
DR A. S. PANTULIJ ( Alternate )
Indi~~~&ltural
DR B. M. LAL
T. K. Nao
SERI V. LAKBBMI~AFTRAN
Saar S. RAMASWAM*

Khadi

!jHRT

and Village

Alternote)

Research

Institute

Industries

Directorate
General
New Delhi

of

Commission,

Technical

( ICAR
Bombay

Development

SR~I S. N. ACJQARWAL ( Altermats )

Central Warehousing
Corporation,
New Delhi
SHRI P. R. VISEAMBHAHAN ( Altnmatc)
Shriram Foods & Fertilizer Industries, New Delhi
SHEI S. C. SINGHAL
Sasr T. N. AMARWAL ( Alicrnafr )
Solvent Extractors Association of India, Bombay
SERI G. V. S~KUR
SHUI L. KRISRAN KUYA~ ( Alternafc )
DR ( SERI~~ATI ) KAMLA SORNIE Consumer Guidance Society of India, Bombay
Regional
Research
Laboratory
( CSIR ), Bhl
Da C. SRINIVASVLU
baneshwar
DR ( SERIWA+TI) K. VIJAYALAXBEIYI ( Alternate )
Oil Technological
Research Institute, Anantapur
Smu S. D. THIRVYWA RAO
Saar G. AZEEMODDIN( Alteraate )
Directorate of Oilseeds Development,
Hyderabad
SEBI M. D. WASNIX
SERI S. P. JUYAL ( Altnnnta )
Director General, IS1 ( Er-o@io Member
)
SERI S. K. MATPUB,
Director ( Chem )

SHRI P. RAMDAS

secretary
SHBI P. S. AROMA
Senior Deputy &rector ( Chem ), IS1

Oils and Fats Subcommittee,

CAFDC

5 : 1

Gmrmn

DR G. LAIWCMINARAYANA

Regional

Research

Laboratory

( CSIR ), Hyderabad

S-I

GOKULCA *ND AQRAWAL

Bombay Oilseeds and Oils Exchange Ltd, Bombay
CEARANDAS
V. MAIUWALA ( Alternuts)
AQRIOULTVRAL MARKETING AD- Directorate of Marketing and Inspection, Nagpur
mama TO THE GOVERNMENT OF
INDIA
SRRI T. V. MATHEW ( Altemotc )
SHRI J. A. ASHTAPVTRE
Miniiry
of Defence ( DGI )
SHKI P. K. MAJVMDAR ( Altemote )
SEEI V. K.BANHAL
Cent;JmOd&zation
for Oil Industry and Trad
!&LI

SHRI H. P. G~PTA ( Alfrrmis )

DR N. V. BRIXWI
Hindustan
DR V. V. S. MANX ( Altmrotr )

Lever

Ltd, Bombay
( Canthud on file

7)

IS : 548 ( Part 2/Set 20 ) - 1983

Indian Standard
METHODS OF
SAMPLING AND TESTS FOR O-ILS AND FATS
PART
Section 20

PURITY

lest for Detection of

TESTS

TARAMIRA

Oil in Mustard/

Rapeseed Oil

0.

FOREWORD

This Indian Standard

( Part e/Section
20 ) was adopted
by the
Indian
Standards Institution on 16 August 1983, after the draft finaILed
by the Oils and Ojlseeds Sectional Committee
had been approved
by the
Chemical
Division
Council
and the Agricultural
and Food Products
Division Council.

0.1

0.2 Methods of sampling and tests for oils and fats ( IS : 548 ) was first
published in 1954 and subsequently
revised in 1964 as Part 1 and it
covered methods of sampling, physical, chemical and qualitative tests.
0.2.1 In view of periodical review of qualitative tests for detection of
adulteration
in oils and fats, the concerned
Sectional Committee
decided
to cover such tests in Part 2 of this stiindard and IS : 548 ( Part 2 )1976* was accordingly
published.
0.2.2 The Sectional Committee
felt that additional
purity tests should
be covered in the form of separate test methods and should not be added
as amendments
to the standard
( that is Part 2 ) since it would create
confusion. The tests covered under various Sections of IS : 548 ( Part 2 )1976 are as follows:
Section

Test for the presence

test );

of sesame

Section
Section

Test for the presence

of

Test for
test );

Section

Test for the presence of Kuranja ( fzungem ) oil and

oils containing
phenolic sulk&es;

Section

10

*Methoda

the

presence

Test for tlke presence

tographic- nlethod;

of sampling

oil ( modified

cottonseed
of

linseed

of argemone

and tests for oils and fats:

3

Part

Baudouin

oil ( Halphen
oil

oil by paper

2 Purity

test );

( Hexabromide

tats.

other

chroma-

IS : 548 ( Part P/Set 20 ) - 1983

Section

11

Test for the presence

of hydrocyanic

Section

12

Test

of mineral

Section

13

Test for the presence of ground nut oil [ Bellier turbidity

temperature
test ( Acetic acid method ) 1;

Section

14

Test for the presence

taining cyanogenic

of l&sum oil and

compounds;

Section

15

Test

of castor

for the presence

for the presence

Section

16

Test

for the presence

Section

17

Test

for the presence

Section

18

Test for the presence

( phytosterol acetate

Section

19

Test for the presence

acid;

oil;

other

oils

con-

oil;

of Neem &I;
of other oils in castor

oil;

of animal
fat in vegetable
melting point test ); and

oil

of oil soluble colours.

the result of a test or analysis made in accordance

with
0.3 In reporting
this standard,
if the final value, observed or calculated,
is to be rounded
off, it shall be done in accordance
with IS : 2-1960*.

1. SCOPE
1.1 This
detection

standard ( Part 2 /Section 20 ) prescribes the method

of TARAMIRA
oil in mustardlrapeseed
oil.

2. OUTLINE

OF THE

of test for

METHOD

2.1 The method involves the isolation of total volatile oil by steam distillation process, its extraction with ethyl ether, concentration
and spotting
on TLC plates are developed in suitable solvent system and sprayed with
ammoniacal
silver nitrate.
Specific spots due to TARAMIRA
oil appear
in addition to the spots due to allyl-isothiocyanate
which is also present
in mustard, rapeseed oil.
3. APPARATUS
3.1 Distillation
distillation.
3.2

Steam

3.5

1-litre,

and

accessories

Generator

3.3 Measuring
3.4 Conical

Flask -

Flask

250-ml.

Flask -lOO-ml.

Separating

Funnel

500-ml.

*Rules for rounding off numerical values ( reuised ).

for

the

purpose

of

IS : 548 ( Part 2jSec 20 ) - 1983

3.6 Sprayer with Rubber Bulb
3.7 TLC Plates -

20 x 10 cm and a suitable

3.8 TLC Applicator

with measuring

3.9 Porcelain Dishes -

50-ml

developing

chamber.

blades.

each.

4. REAGENTS
4.1 Silver Nitrate Solution -

25 percent

in water.

4.2 Ammoniacal Silver Nitrate Solution - 2.5 ml of the 25 percent

silver nitrate solution ( see 4.1 ) is made up to 100 ml with liquor
ammonia.
This solution is to be used as a spraying agents.
4.3

Silica Gel -

4.4

Benzene

of suitable

grade*

with calcium

sulphate

as binder.

4.5 Absolute Alcohol

4.6 Liquor Ammonia
4.7

relative

density

0.88.

Solvent Ether

4.8 Solvent System 5. PREPARATION

Benzene

: absolute

alcohol

( 94 : 6 ).

OF TLC PLATES

5.1 Suitable

amount of silica gel at the rate of 5 g per plate is mixed well

The
with its double amount of water in a conical flask for 30 seconds.
thickness of the applicator
is preadjusted
at about 400 pm.
The plates
The plates are then
are coated as usual and allowed to dry under fan.
dried/activated
at 105C for one hour before use.
6. PROCEDURE

6.1 Take 5 ml of oil into a distillation flask and add about 200 ml of
Connect
with flask to the steam generator and distil the
water
to it.
total volatile
oil.
Collect
about
150-160 ml of the distillate
into a
Transfer the
measuring
flask which contains
10 to 15 ml of water.
distillate to a separating
funnel and extract twice with 25 ml portions
of
solvent ether.
Collect
the extract
into a conical flask and after a few
minutes decant into a porcelain dish taking care not to allow any drop
of water to fall in the dish.
Concentrate
the extract to about 1 ml on a
water bath OI! any alternate
heating
arrangement
the temperature
of
which shall not exceed 60C.
Spot about 15 ~1 of the extract on the
TLC plate and allow the solvent to evaporate.
Place the plates into a
___.
*A suitable grade is kieselgel G.
5

IS i 548 ( Part 2/6ec 20 ) - 1983

developing
chamber
containing
the solvent system ( benzene-absolute
alcohol ) ( see 4.8 ) so as to dip the plates about 1 cm.
After the solvent
mixture
has risen to exactly 10 centimetre height, remove the plates and
allow the solvent to evaporate.
Spray the plates with ammoniacal
silver
nitrate solution and set aside.
Within
10 to 15 minutes brownish spots
appear on the plate.
7. IDENTIFICATION

OF SPOTS

7.1 Specific

greenish brown spots due to TARAMIRA

appears at Rt 0.1
and 0.9. With lower amount the upper spots at Rt 0.9 sometimes may not
appear depending upon the amount of specific sulphur compound present
in the oil.

7.2 Common
black brown spots due to the allyl-isothiocyanate
which
is present both in mustard and TARAMIRA
oils appear at Rt 0.8.

7.3 Some
to be taken

shadow
spots
into account.

at RI 01 to 0.2 may also appear

which

are not

8. SENSITIVITY

8.1 This method is sensitive to detect the presence of TARAMIRA

oil/
powder in mustard or rapeseed oil/powder up to an extent of 2 percent. In
case the particular variety of seeds contain larger amount of the specific
volatile component
the method
would respond to as low as 1 percent
adulteration.
The method, however, depends on the isolation of volatile
activating
the enzyme
myrosinase.
The
solvent
extracted
oil by
TARAMIRA
oil having no characteristic
smell may not respond to this
test.

IS : 548 ( Part 2/Set 20 ) - 1983

( Conthued from page 2 )
RqJre_wnting

Members

Central Committee
for Food Standards ( Ministry
of Health and Family Welfare ), New Delhi
831~ DEBI MURHERJEE ( Alternate )
Vanaspati
Manufacturers
Association
of India,
DR A. C. CHATRAPATI
Bombay
SHXI C. NARAYANASWAMY.( Alternate )
Directorate
General
of Technical
Development,
SHRI R. D. KAWAT~A
New Delhi
Khadi and Village Industries Commission, Bombay
SHRI V. LAKSHMIKANTHAN
SHRI J. N. BHATTACHARYA ( Alternate )
Indian Council of Agricultural Research Institute,
D.R B. M. L~L
New Delhi
Indian Paint Association, Calcutta
SHRI R. K. MARPHATIA
SHRI RABIN SARKAR ( Alternate )
Tata Oil Mills Co Ltd, Bombay
DIE N. L. MURTY
Da A. D. SIIITOLE ( Alternate )
Oil Technologists Association of India, Kanpur
D,t S. M. PATEL
PROF V. V. R. SUURAI~XANYA~I( Alternate )
Central
Food
Technological
Research
Institute
Drt J. V. PR 4112IAXAR
( CSIR ), Mysore
Directorate of \anaspati, Vegetable Oils and Fats
SXRI N. S. RAJAGOP~L
( Ministry of Civil Supplies ), New Delhi
DR 1. A. SIDD~QUI( dllernate )
Indian Confectionery
Manufacturers
Association,
SHRI R. D. SHEN~I
New Delhi
Indian Soap and Toiletries
Makers Association,
SIIRI M. S. THAKUR
Bombav; nnd Godrej Soaps Ltd, Bombay
Oil 7 echnoicgical Research Institute, Anantapur
S~rltr S. D. TJXIRUMALA RAO
S:IILI D. AWHYUTA
RAMAYYA ( &tern& )
Directorate of Oilseeds Development, Hyderabad
S~rnr M. D. WaSNlIi
Snar S. I. JIXAL ( Alternate )
SWAI D. S. CHADHA

Panel

for Detection

of Adulteration

in Vegetable

Oils, CAFDC

5 : 1 : 4

Cmm?ncr
DR G. LAKSRMCNARAYANA

Regional
Research
Hyderabad

Laboratory

(CSIR

),

DR T. N. 11. KAIXAL ( Alternafe to

Dr G. Lakshminarayana )
Director of Health Services. Trivandrum
SMTK. P. A.RI-MA
Hindustan Lever Ltd, Bombay
DIG N. V. Bxrwcr
Dz V. V. S. MANI ( Alternate )
Department of Applied Chemistry,
University
of
I~{oF M. M. CX~KXABAXTY
Calcutta. Calcutta
Drt DIPAK Kunr IR BNATTACRARYYA ( Alfernafe ).
Jamna
La1 Rajaj Research
Institute ( KVIC ),
SERI N. CrrALAPATI Rso
Wardha

( Continued on page a )
7

IS : 548 ( Part 2/&c 20 ) - 1983

( Continued from pcrp~
7)
Rcpestnting

Members

National Dairy Research Institute, Karnal

DR N. C. GANQTJLI
Central Food Laboratory, Calcutta
DR K. C. GUHA
SHRI A. K. DHAR ( Alternate )
Central Agmark Laboratory, Nagpur
SARI T. V. MATHEW
SHRIK. L. CHATTERJEE ( Ahmatc )
Food & Drug Administration
( Government
of
SHRI B. C. MF.BTA
Maharashtra ), Bombay
Central Food
Technological
Research
Institute
DR J. V. PRABHAKAR
( CSIR ), Mysore
University of Bombay, Bombay
DR D. REBELLO
Office of the Public
Analyst
( Government of
DR S. B. SINGE
Uttar Pradesh ), Lucknoy
SHRI M. S. THAXTJR
Godrej Soaps Ltd, Bombay
Oil Technological
Research
Institute, Anantapur
SHRI S. D. TAIRUYALA RAO
SHRI D. ATCHYUTA RAMAYYA ( Altcmatc )

BUREAU

OF

Hesdquerters

iNOlAN

Manak Bhavan. 9 Bahadur &ah

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Central
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Zafar Marg. NEW DELHI 110002

: 331 01 31

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