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Rhinovirus Infections in the

First 2 Years of Life


Laura Toivonen, MD,a,b Linnea Schuez-Havupalo, MD,a,b Sinikka Karppinen, MD,a,b Tamara Teros-Jaakkola, MD,a,b
Maris Rulli, MD, PhD,a,b Jussi Mertsola, MD, PhD,a,b Matti Waris, PhD,c Ville Peltola, MD, PhDa,b

BACKGROUND AND OBJECTIVES: Rhinoviruses

frequently cause respiratory infections in young children. We


aimed to establish the burden of acute respiratory infections caused by rhinovirus during the
first 2 years of life.
LATAR BELAKANG DAN TUJUAN: Rhinoviruses sering menyebabkan infeksi pernafasan pada
anak-anak. Kami bertujuan untuk membangun beban infeksi pernapasan akut yang
disebabkan oleh rhinovirus selama 2 tahun pertama kehidupan
METHODS: In

this prospective birth cohort study, we followed 923 children for acute respiratory
infections from birth to 2 years of age. Data on respiratory infections were collected by daily
symptom diaries, study clinic visits, and from electronic registries. Respiratory viruses were
detected by reverse transcription-polymerase chain reaction and antigen assays during respiratory
infections and at the age of 2, 13, and 24 months. The rates of rhinovirus infections and associated
morbidities were determined.
METODE: Dalam penelitian prospektif kelompok kelahiran ini, kami mengikuti 923 anakanak untuk infeksi saluran pernapasan akut dari lahir sampai 2 tahun. Data pada
infeksi pernafasan dikumpulkan oleh catatan harian gejala, kunjungan klinik studi, dan
dari pendaftar elektronik. virus pernapasan terdeteksi oleh reaksi berantai
transkripsi-polimerase terbalik dan tes antigen selama infeksi pernapasan dan pada
usia 2, 13, dan 24 bulan. Tingkat infeksi rhinovirus dan morbiditas terkait
ditentukan.
We documented 8847 episodes of acute respiratory infections, with an annual rate of 5.9
per child (95% confidence interval [CI], 5.76.1). Rhinovirus was detected in 59% of acute
respiratory infections analyzed for viruses. Rhinovirus was associated with 50% of acute otitis
media episodes, 41% of wheezing illnesses, 49% of antibiotic treatments, and
48% of outpatient ofice visits for acute respiratory infections. The estimated mean annual rate of
rhinovirus infections was 3.5 per child (95% CI, 3.33.6), 47 per 100 children (95% CI, 4252) for
rhinovirus-associated acute otitis media, and 61 per 100 children (95% CI,
5568) for rhinovirus-associated antibiotic treatment. The prevalence of rhinovirus at 2,
13, or 24 months of age was 14 to 24%, and 9% of asymptomatic children were positive for
rhinovirus.
HASIL: Kami didokumentasikan 8847 episode infeksi pernafasan akut, dengan tingkat
tahunan 5,9 per anak (95% confidence interval [CI], 5,7-6,1). Rhinovirus terdeteksi
pada 59% dari infeksi saluran pernapasan akut dianalisis untuk virus. Rhinovirus
dikaitkan dengan 50% dari episode otitis media akut, 41% dari penyakit mengi, 49% dari
perawatan antibiotik, dan
48% dari kantor rawat jalan kunjungan untuk infeksi saluran pernapasan akut.
Diperkirakan tingkat tahunan rata-rata infeksi rhinovirus adalah 3,5 per anak (95% CI,
3,3-3,6), 47 per 100 anak (95% CI, 42-52) untuk rhinovirus terkait otitis media akut,
dan 61 per 100 anak (95 % CI,
55-68) untuk pengobatan antibiotik rhinovirus terkait. Prevalensi rhinovirus pada 2,
13, atau 24 bulan usia adalah 14 sampai 24%, dan 9% dari anak-anak tanpa gejala
positif untuk rhinovirus.
RESULTS:

CONCLUSIONS: Rhinovirus

infections impose a major burden of acute respiratory illness and antibiotic


use on young children.
KESIMPULAN: infeksi Rhinovirus memaksakan beban utama penyakit pernapasan akut dan
penggunaan antibiotik pada anak-anak.

abstract

Department of Pediatrics and Adolescent Medicine, Turku University Hospital, bTurku Institute for Child and
Youth Research, and cDepartment of Virology, University of Turku, Turku, Finland

Dr Toivonen contributed to the clinical follow-up and data collection, carried out data analyses, and drafted the initial manuscript; Drs Schuez-Havupalo, Karppinen,
Teros-Jaakkola, and Rulli contributed to the clinical follow-up and data collection and reviewed and revised the manuscript; Dr Mertsola contributed to the study
design and reviewed and revised the manuscript; Dr Waris contributed to the study design, carried out virological analyses, and reviewed and revised the manuscript;
Dr Peltola conceptualized and designed the study, coordinated and supervised data collection and analyses, and reviewed and revised the manuscript; and all authors
approved the nal manuscript as submitted and agree to be accountable for all aspects of the work.
DOI: 10.1542/peds.2016-1309
Accepted for publication Jun 21, 2016
Address correspondence to Ville Peltola, MD, PhD, Department of Pediatrics and Adolescent
Medicine, Turku University Hospital, 20521 Turku, Finland. E-mail: ville.peltola@utu.
PEDIATRICS (ISSN Numbers: Print, 0031-4005; Online, 1098-4275).

WHATS KNOWN ON THIS SUBJECT: Rhinoviruses commonly cause respiratory infections ranging
from asymptomatic infections and the common cold to asthma exacerbations and severe respiratory diseases
requiring hospitalization. However, the burden of rhinovirus infections in young children
has not been comprehensively described.
APA YANG DIKENAL DENGAN TOPIK: Rhinoviruses sering menyebabkan infeksi saluran
pernapasan mulai
dari infeksi tanpa gejala dan flu biasa untuk eksaserbasi asma dan penyakit pernafasan
parah yang membutuhkan rawat inap. Namun, beban infeksi rhinovirus pada anak-anak
belum komprehensif dijelaskan.
WHAT THIS STUDY ADDS: Rhinovirus infections impose a major burden on young children with an estimated mean annual rate of 3.5 in
children aged
0 to 23 months. Rhinoviruses associate with half of acute otitis media episodes, antibiotic treatments, and outpatient visits for acute
respiratory infections.

APA STUDI INI ADDS: infeksi Rhinovirus memaksakan beban besar pada anak-anak dengan
tingkat tahunan rata-rata estimasi 3,5 pada anak-anak usia
0 sampai 23 bulan. Rhinovirus asosiasi dengan setengah dari episode akut otitis media,
perawatan antibiotik, dan kunjungan rawat jalan untuk infeksi saluran pernapasan akut.
Copyright 2016 by the American Academy of Pediatrics

PEDIATRICS Volume 138, number 3, September 2016:e20161309

To cite: Toivonen L, Schuez-Havupalo L, Karppinen S, et al.


Rhinovirus Infections in the First 2 Years of Life. Pediatrics.
2016;138(3):e20161309

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ARTICLE

Acute respiratory infections are a major cause of morbidity in infants and young children, and rhinovirus
is the most common causative agent.13
Clinical manifestations of rhinovirus infection range from asymptomatic infections and the common cold
to asthma exacerbations and severe respiratory diseases requiring hospitalization.48

infeksi saluran pernafasan akut adalah penyebab utama morbiditas pada bayi dan anakanak, dan rhinovirus adalah agent.1-3 penyebab paling umum
Manifestasi klinis dari berbagai infeksi rhinovirus dari infeksi tanpa gejala dan
pilek untuk eksaserbasi asma dan penyakit pernafasan parah yang membutuhkan
hospitalization.4-8
With the use of sensitive polymerase chain reaction (PCR) and other molecular assays for diagnosis and
typing, the detection rates of rhinoviruses have increased, group A, B, and C rhinoviruses have been
identified, and the spectrum
of rhinovirus illnesses has been better recognized.9

Dengan penggunaan sensitif polymerase chain reaction (PCR) dan tes molekul lain
untuk diagnosis dan mengetik, tingkat deteksi dari rhinovirus meningkat, kelompok A,
B, dan C rhinovirus telah diidentifikasi, dan spektrum
penyakit rhinovirus telah recognized.9 lebih baik
However, the illness burden of rhinovirus infections in young children is poorly defined. Previous
studies have been either small, conducted
in selected populations, done before the era of PCR diagnostics, or they have not covered
noncomplicated upper respiratory tract infections that may contribute substantially to the disease
burden because of high frequency.3,1017

Namun, beban penyakit infeksi rhinovirus pada anak-anak yang kurang jelas. Studi
sebelumnya telah baik kecil, dilakukan
pada populasi yang dipilih, dilakukan sebelum era PCR diagnostik, atau mereka tidak
ditutupi infeksi saluran tanpa komplikasi pernapasan atas yang dapat berkontribusi
besar terhadap beban penyakit karena frequency.3,10-17 tinggi
We followed up on the occurrence and clinical outcomes of acute respiratory infections caused by
rhinovirus in a cohort of children from birth to 2 years of age. In addition to nasal swab sampling
at the study clinic, we used home sampling by parents. We also determined the prevalence of
rhinovirus at the age of 2, 13, and
24 months and the presence of associated symptoms.

Kami menindaklanjuti terjadinya dan hasil klinis infeksi pernapasan akut yang
disebabkan oleh rhinovirus dalam kelompok anak-anak sejak lahir sampai 2 tahun.
Selain hidung swab pengambilan sampel
di klinik studi, kami menggunakan rumah sampling dengan orang tua. Kami juga
menentukan prevalensi rhinovirus pada usia 2, 13, dan
24 bulan dan adanya gejala yang terkait.
METHODS

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TOIVONEN et al

FIGURE 1

Enrolment and follow-up of the study children. Follow-up was considered complete at the age of 12
and 24 months if the symptom diary was lled, or if there were records of outpatient clinic visits or nasal swab specimens at the age of 10 or 22
months, respectively.

Study Design and Conduct


From all children born in the Hospital District of Southwest Finland from January 2008 through April 2010
to Finnish or Swedish-speaking mothers (eligible cohort, n = 9811 mothers; n = 9936 children), families
of 1827 children (30 pairs of twins) were recruited either during the
first trimester of pregnancy or

Studi Desain dan Perilaku


Dari semua anak yang lahir di Kabupaten Rumah Sakit Southwest Finlandia dari Januari
2008 sampai April 2010
ibu Finlandia atau berbahasa Swedia (kohort memenuhi syarat, n = 9811 ibu; n = 9936
anak-anak), keluarga dari 1.827 anak-anak (30 pasang kembar) direkrut baik selama
trimester pertama kehamilan atau

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PEDIATRICS Volume 138, number 3, September 2016

soon after birth in the prospective,


observational birth cohort study, Steps to the Healthy Development
and Well-being of Children (STEPS).18
Parents of all children recruited for the STEPS Study were asked about participation of the child in an
intensive follow-up of respiratory infections from birth to 2 years of age, and 982 children participated
(Fig 1). No selection criteria other

segera setelah lahir di calon,


observasional studi kelompok kelahiran, Langkah-langkah untuk Pembangunan Kesehatan
dan Kesejahteraan Anak (LANGKAH) .18
Orang tua dari anak-anak direkrut untuk LANGKAH Studi ditanya tentang partisipasi
anak dalam intensif tindak lanjut dari infeksi pernapasan dari lahir sampai 2 tahun,
dan 982 anak-anak berpartisipasi (Gambar 1). Tidak ada kriteria seleksi lainnya

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TOIVONEN et al

than language were applied with


regard to the recruitment of families for the STEPS Study or for the subcohort with infection follow-up.
The follow-up was conducted from April 2008 to April 2012. Parents were asked to record all respiratory
and other symptoms, physician visits with associated diagnoses
and treatments, and illness-related

dari bahasa diaplikasikan dengan


Berkenaan dengan perekrutan keluarga untuk LANGKAH Studi atau untuk sub-kohort
dengan infeksi tindak lanjut.
Tindak lanjut dilakukan dari bulan April 2008 sampai April 2012. Orang tua diminta
untuk mencatat semua pernapasan dan gejala lain, dokter kunjungan dengan diagnosa
terkait
dan perawatan, dan penyakit terkait

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PEDIATRICS Volume 138, number 3, September 2016

absenteeism of the child from day care and the parent from work into a daily symptom diary.
Background information was gathered by structured question forms before birth or soon after birth, and
information on day care attendance was collected at ages 13, 18, and 24 months.

ketidakhadiran anak dari penitipan dan orang tua dari pekerjaan menjadi buku harian
gejala sehari-hari. informasi latar belakang yang dikumpulkan oleh bentuk pertanyaan
terstruktur sebelum lahir atau segera setelah lahir, dan informasi di kehadiran
penitipan dikumpulkan pada usia 13, 18, dan 24 bulan.
Families were encouraged to visit the study clinic whenever the child experienced an acute
respiratory infection and parents believed that an evaluation by a
physician was needed. At the study clinic, children were examined
by a study physician and clinical findings were documented using a structured form. Pneumatic
otoscopy and tympanometry were used for diagnosing otitis media. Nasal swabs from both nostrils at a
depth of 2 to 3 cm were obtained by using flocked nylon swabs (Copan, Brescia, Italy) by the study
physician.

Keluarga didorong untuk mengunjungi klinik penelitian setiap kali anak mengalami
infeksi saluran pernafasan akut dan orang tua percaya bahwa evaluasi oleh
dokter diperlukan. Di klinik studi, anak-anak diperiksa
oleh dokter studi dan temuan klinis didokumentasikan menggunakan bentuk terstruktur.
pneumatic
otoscopy dan timpanometri digunakan untuk mendiagnosa otitis media. penyeka hidung
dari kedua lubang hidung pada kedalaman 2 sampai 3 cm diperoleh dengan menggunakan
berbondong-bondong penyeka nilon (Copan, Brescia, Italia) oleh dokter studi.
Alternatively, if the family felt that a physician visit was not needed, or if they visited a physician
elsewhere, nasal flocked swab specimens were taken at home by the parents at
the onset of illness and sent to the laboratory by standard mail as described previously.19,20 Parents
were trained by study personnel to collect nasal swab samples at the first visit to the study clinic. Data
on emergency department visits and hospitalizations of the child, including results of virus diagnostics
performed as part of patient care, were collected from the Electronic Registry of the Hospital District of
Southwest Finland, which comprises information from both hospitals providing inpatient pediatric care in
the area (Turku University Hospital and Salo District Hospital).

Atau, jika keluarga merasa bahwa kunjungan dokter tidak diperlukan, atau jika mereka
mengunjungi dokter di tempat lain, hidung berbondong-bondong spesimen swab diambil
di rumah oleh orang tua di
timbulnya penyakit dan dikirim ke laboratorium melalui surat standar seperti orang
tua previously.19,20 dijelaskan dilatih oleh personel studi untuk mengumpulkan
sampel swab hidung pada kunjungan pertama ke klinik studi. Data
pada kunjungan gawat darurat dan rawat inap anak, termasuk hasil diagnosa virus
dilakukan sebagai bagian dari perawatan pasien, dikumpulkan dari Registry Elektronik
Kabupaten Rumah Sakit Southwest Finlandia, yang terdiri dari informasi dari kedua
rumah sakit menyediakan perawatan anak rawat inap di daerah ( Rumah Sakit
Universitas Turku dan RSUD Salo)
Children were invited to visit the study clinic at the age of 2, 13, and
24 months. Any current respiratory symptoms were documented and nasal swab specimens were
taken.
The Ministry of Social Afairs and
Health and the ethics committee of
the Hospital District of Southwest Finland approved the STEPS Study. Parents of participating children
gave their written, informed consent.

Anak-anak diajak untuk mengunjungi klinik studi pada usia 2, 13, dan
24 bulan. Apa saja gejala pernapasan saat didokumentasikan dan spesimen swab hidung
diambil.
Departemen Sosial dan
Kesehatan dan komite etika
Distrik Rumah Sakit Southwest Finlandia menyetujui LANGKAH Study. Orang tua dari
anak-anak yang berpartisipasi memberi tertulis, persetujuan mereka diberitahu.

Respiratory Virus Detection


The nasal swab specimens were stored at 80C before analysis. Swabs were suspended in phosphate
bufered saline and nucleic acids were extracted by NucliSense easyMag (BioMerieux, Boxtel,
the Netherlands) or MagnaPure
96 (Roche, Penzberg, Germany) automated extractor.

Spesimen swab hidung disimpan pada -80 C sebelum analisis. Penyeka dihentikan pada
fosfat buffered saline dan asam nukleat diekstraksi oleh NucliSense easyMag
(BIOMERIEUX, Boxtel,
Belanda) atau MagnaPure
96 (Roche, Penzberg, Jerman) extractor otomatis
Extracted RNA was reverse transcribed and the complementary DNA was amplified using real-time,
quantitative PCR for rhinovirus, human enteroviruses, and respiratory syncytial virus
(RSV) as described previously (all samples).21,22 Laboratory- developed antigen detection tests were
performed for influenza A and B viruses, parainfluenza type 1, 2, and 3 viruses, RSV, adenovirus, and
human metapneumovirus
for samples collected in January 2009 or later (89% of samples).

Diekstraksi RNA sebaliknya ditranskrip dan DNA komplementer diamplifikasi


menggunakan real-time, kuantitatif PCR untuk rhinovirus, enterovirus manusia, dan
virus pernapasan
(RSV) seperti yang dijelaskan sebelumnya (semua sampel) .21,22 laboratoriummengembangkan tes deteksi antigen dilakukan untuk influenza A dan B virus,
parainfluenza tipe 1, 2, dan 3 virus, RSV, adenovirus, dan metapneumovirus manusia
untuk sampel yang dikumpulkan pada bulan Januari 2009 atau lambat (89% dari sampel).
All specimens collected during influenza seasons were subjected to reverse transcription-PCR for
influenza A
and B viruses.23 The first and last day of each influenza season were defined on the basis of the
influenza antigen test results and data from the infectious disease surveillance registry of the National
Institute for
Health and Welfare, Finland. Routine respiratory viral diagnostics were performed by PCR or rapid
antigen detection methods for 26 patients as part of their care at the Turku University Hospital.

Semua spesimen dikumpulkan selama musim influenza menjadi sasaran reverse


transkripsi-PCR untuk influenza A
dan B viruses.23 Hari pertama dan terakhir setiap musim influenza didefinisikan atas
dasar hasil tes antigen influenza dan data dari registri surveilans penyakit menular
dari Institut Nasional untuk
Kesehatan dan Kesejahteraan, Finlandia. Rutin diagnostik virus pernapasan dilakukan
dengan PCR atau metode deteksi antigen cepat untuk 26 pasien sebagai bagian dari
perawatan mereka di Rumah Sakit Turku University.
Statistical Analysis
An episode of acute respiratory infection was defined as the presence of rhinitis or cough, with or without
fever or wheezing, documented in the diary by parents, or as a diagnosis of an acute respiratory infection
by a physician.
If several nasal specimens were collected during continuous symptoms, nasal swabs taken
within 14 days were considered as 1 episode, and the date of a new nasal swab taken >14 days from
the earlier sample was considered as the first day of a new episode.

Sebuah episode infeksi saluran pernafasan akut didefinisikan sebagai keberadaan


rhinitis atau batuk, dengan atau tanpa demam atau mengi, didokumentasikan dalam buku
harian oleh orang tua, atau sebagai diagnosis infeksi saluran pernafasan akut oleh
dokter.
Jika beberapa spesimen hidung dikumpulkan selama gejala terus menerus, penyeka
hidung diambil

dalam waktu 14 hari dianggap sebagai 1 episode, dan tanggal dari swab hidung baru
diambil> 14 hari dari sampel sebelumnya dianggap sebagai hari pertama dari episode
baru.

Coinfections were defined as detection of 2 viruses from 1 or several specimens during the same
episode within 14 days from each other. If there were repeated diagnoses of acute otitis media,
wheezing illness, pneumonia, laryngitis, or pharyngitis during continuous respiratory symptoms,
diagnoses within 14 days were calculated as 1 episode. If the diary data were missing, outpatient visits
and hospitalizations for acute respiratory infection 1 day before through 14 days after the nasal swab
collection were linked with the virologic result.

Koinfeksi didefinisikan sebagai deteksi 2 virus dari 1 atau beberapa spesimen


selama episode yang sama dalam waktu 14 hari dari satu sama lain. Jika ada diagnosa
berulang otitis media akut, mengi penyakit, pneumonia, radang tenggorokan, atau
faringitis selama gejala pernapasan yang berkelanjutan, diagnosis dalam waktu 14
hari dihitung sebagai 1 episode. Jika data buku harian yang hilang, kunjungan rawat
jalan dan rawat inap untuk infeksi saluran pernapasan akut 1 hari sebelum sampai 14
hari setelah koleksi swab hidung dikaitkan dengan hasil virologi.
When calculating the annual respiratory illness days associated with rhinovirus infections, the length of
an acute respiratory infection was limited to a maximum of 60 days. When analyzing other
characteristics and outcomes of virus infections, data were included from the symptomatic period with
a maximum duration of 14 days before and 14 days after collection of the nasal sample. Coinfections
involving rhinoviruses were excluded from analyses of characteristics of rhinovirus infections because
of the unclear role of diferent viruses in the pathogenesis of coinfections
.

Ketika menghitung hari penyakit pernapasan tahunan yang terkait dengan infeksi
rhinovirus, panjang infeksi saluran pernapasan akut dibatasi maksimum 60 hari.
Ketika menganalisis karakteristik dan hasil dari infeksi virus lain, data dimasukkan
dari periode gejala dengan durasi maksimal 14 hari sebelum dan 14 hari setelah
pengumpulan sampel hidung. Koinfeksi melibatkan rhinovirus dikeluarkan dari analisis
karakteristik infeksi rhinovirus karena peran jelas dari virus yang berbeda dalam
patogenesis koinfeksi
.
Categorical data were compared by using the 2 test. Continuous data were described and compared by
using means, SDs, or 95% confidence intervals (CI), and Students t test, or medians, interquartile
ranges (IQR), and MannWhitney U test, as appropriate. The proportion of children positive for rhinovirus
at 2, 13, and 24 months of age was compared with the Friedman test. Generalized linear models were
used to describe and analyze rates

Data kategori dibandingkan dengan menggunakan uji 2. Data kontinyu digambarkan dan
dibandingkan dengan menggunakan cara, SD, atau 95% confidence interval (CI), dan uji
t Student, atau median, rentang antar-kuartil (IQR), dan Mann-Whitney U test, yang
sesuai. Proporsi anak yang positif untuk rhinovirus pada 2, 13, dan 24 bulan usia
dibandingkan dengan uji Friedman. model linear umum yang digunakan untuk
menggambarkan dan menganalisis tingkat

TABLE 1 Baseline Characteristics of the Study Children


Characteristic

Study Children, n (%)


(N = 923)

Female
Older siblings
Mothers educational level at least professional
Living in the urban area
Mother smoked during pregnancy
Parent smoked during pregnancy
Breast-fed for at least 6 mo
Attendance at outside-home day care
At the age of 13 mo
At the age of 18 mo
At the age of 24 mo
Vaccinated against inuenzaa
20082009
20092010, with seasonal inuenza vaccine
20092010, with pandemic inuenza vaccine
20102011
20112012
a

435 (47)
376 (41)
574/892 (64)
544/895 (61)
44/801 (5)
141/818 (17)
432/716 (60)

Incidence and Characteristics of


Rhinovirus Infections

185/784 (24)
285/685 (42)
370/681 (54)
13/59 (22)
173/366 (47)
296/366 (81)
130/371 (35)
12/54 (22)

Calculated among children 6 mo of age actively followed-up at the beginning of each inuenza season.

for rhinovirus-associated outcomes.


Outcome counts were analyzed by
using negative binomial distribution
and log link with natural logarithm
of follow-up time as an ofset
variable. Pneumonia counts were
low and negative binomial regressionfailed; thus, for pneumonia, Poisson
distribution was used instead.
untuk hasil rhinovirus terkait.
jumlah hasil dianalisis dengan
menggunakan distribusi binomial negatif
dan log hubungan dengan logaritma natural
waktu tindak lanjut sebagai offset
variabel. jumlah Pneumonia adalah
rendah dan negatif regressionfailed binomial; dengan demikian, untuk pneumonia,
Poisson
distribusi digunakan sebagai gantinya.
of rhinovirus-positive infections from
infections with a nasal swab analyzed
for viruses. The number of rhinovirusassociated
acute respiratory infections was
determined as a product of the proportion
of rhinovirus infections and
total infections.
infeksi rhinovirus-positif dari
infeksi dengan swab hidung dianalisis
untuk virus. Jumlah rhinovirusassociated
infeksi saluran pernapasan akut adalah ditentukan sebagai produk dari proporsi
infeksi rhinovirus dan infeksi Total

70% of swabs being taken at


home by parents.
Characteristics of

acute respiratory infections


with
and without a nasal sample
are
compared in Supplemental
Table 5.

70% dari penyeka yang diambil di rumah oleh orang tua. Karakter dari
infeksi saluran pernapasan akut dengan
dan tanpa sampel hidung yang
dibandingkan di Tambahan Tabel 5.
Rhinovirus was detected in
59% of
acute respiratory infections
with
a specimen analyzed for
viruses
(Table 2). RSV was detected
in
6% and 1 of the other
respiratory
viruses studied in 5% of
acute
respiratory infections.
Coinfections
were confirmed in 2% of
episodes,
and 29% remained negative
in
virologic analysis. Symptoms,

Rhinovirus terdeteksi pada 59% dari


infeksi saluran pernapasan akut dengan
spesimen dianalisis untuk virus
(Tabel 2). RSV terdeteksi di
6% dan 1 dari pernapasan lainnya
virus ini dapat dipelajari dalam 5% dari akut
infeksi saluran pernapasan. koinfeksi
dikonfirmasi di 2% dari episode,
dan 29% tetap negatif di
analisis virologi. gejala,
Cls for product were detemined by using bootstrap sampel. P value <0.05 wee considered statistically
significant. The data were anallyzed wit the use spss software version d SAS software for Windows,

version 9.4 (SAS Institute, Inc, Cary,


NC).
Cls untuk produk yang detemined dengan menggunakan sampel bootstrap. Nilai P <0,05
wee dianggap signifikan secara statistik. Data anallyzed wit versi penggunaan
software SPSS d software SAS untuk Windows,
versi 9.4 (SAS Institute, Inc, Cary,
NC).

RESULTS
Study Population
A total of 982 children were recruited for follow-up of respiratory infections. Fifty-nine children did not
start the follow-up, resulting in the study group of 923 children (Fig 1, Table 1). Data on acute respiratory
infections were received from 95% of these children, and the mean duration of the follow-up was 1.69
(SD, 0.47) years. Based on data from the Finnish National Birth Registry, 55% of mothers in the eligible
cohort (n = 9811) had 1 children.18 Thus, our study population with 41% of children having 1 older
siblings was skewed toward including more first- born children than the general child population.

Sebanyak 982 anak-anak direkrut untuk tindak lanjut dari infeksi pernapasan. Lima
puluh sembilan anak tidak
mulai tindak lanjut, sehingga dalam kelompok studi dari 923 anak-anak (Gambar 1,
Tabel 1). Data pada infeksi saluran pernafasan akut yang diterima dari 95% dari

anak-anak ini, dan durasi rata-rata tindak lanjut adalah 1,69 (SD, 0,47) tahun.
Berdasarkan data dari Registry Kelahiran Nasional Finlandia, 55% dari ibu dalam
kelompok yang memenuhi syarat (n = 9811) memiliki 1 children.18 demikian, populasi
penelitian kami dengan 41% dari anak-anak memiliki 1 tua saudara miring ke arah
termasuk lebih dulu - lahir anak dari populasi anak-anak umum
Documentation of Acute Respiratory
Infections
A total of 8847 episodes of acute respiratory infection, causing 4691 outpatient visits and 85
hospitalizations, were documented. Of all outpatient visits, 2142 (46%) occurred at the study clinic,
2338
(50%) at other outpatient clinics or physician offices, and 211 (4%) at the emergency department.
Antibiotics were prescribed less frequently at the study clinic (in 29% of episodes) compared with
other sites (in 50% of episodes, P < .001). A nasal swab sample was collected during 4728 (53%)
acute respiratory infections with 70% of swabs being taken at home by parents. Characteristics of
acute respiratory infections with and without a nasal sample are compared in Supplemental Table 5.
Dokumentasi Saluran Pernafasan Akut
infeksi
Sebanyak 8847 episode infeksi pernafasan akut, menyebabkan 4.691 kunjungan rawat
jalan dan 85
rawat inap, didokumentasikan. Dari semua kunjungan rawat jalan, 2.142 (46%) terjadi
di klinik studi, 2338
(50%) di klinik lainnya rawat jalan atau kantor dokter, dan 211 (4%) di departemen
darurat. Antibiotik yang diresepkan jarang di klinik studi (di 29% dari episode)
dibandingkan dengan
situs lain (di 50% dari episode, P <0,001). Sampel swab hidung dikumpulkan selama
4728 (53%) infeksi saluran pernafasan akut dengan 70% dari penyeka yang diambil di
rumah oleh orang tua. Karakteristik infeksi saluran pernafasan akut dengan dan tanpa
sampel hidung dibandingkan dalam Tambahan Tabel 5.
Incidence and Characteristics of
Rhinovirus Infections
Rhinovirus was detected in 59% ofacute respiratory infections with da specimen analyzed for
viruses
( Table 2). RSV was detected in
6% and 1 of the other respiratory
viruses studied in 5% of acute
respiratory infections. Coinfectionswere confirmed in 2% of episodes,and 29% remained negative in
virologic analysis. Symptoms,
diagnoses, and treatment of acute
respiratory infections positive
or negative for rhinovirus are
compared in Table 3. Children
with an acute respiratory infectionpositive for rhinovirus experiencedfever, cough, poor appetite, or
gastrointestinal symptoms less
frequently than those with an acuterespiratory infection negative for
rhinovirus.
Rates of Acute Respiratory
Infections
The mean annual rate of all acute respiratory infections, including those lacking a specimen for
virologic analysis, was 5.9 per child (95% CI, 5.76.1) (Table 4). The rate of acute respiratory
infections positive for rhinovirus was 2.0
(95% CI, 1.92.1). Assuming that the proportion of rhinovirus infections
is similar in infections not analyzed as in those analyzed for viruses, we estimated that the annual
rate of acute respiratory infections caused by rhinovirus was 3.5 per child (95% CI, 3.33.6) in
children <2 years of age. Rhinovirus infections were estimated to cause a mean of

31 respiratory illness days per child annually. The rate of rhinovirus infections was highest among
children 6 to 23 months of age, but noticeably high already before 6 months of age.

Tarif Saluran Pernafasan Akut


infeksi
Tingkat tahunan rata-rata dari semua infeksi pernapasan akut, termasuk yang kurang
spesimen untuk analisis virologi, adalah 5,9 per anak (95% CI, 5,7-6,1) (Tabel 4).
Tingkat infeksi saluran pernapasan akut positif rhinovirus adalah 2.0
(95% CI, 1,9-2,1). Dengan asumsi bahwa proporsi infeksi rhinovirus
mirip infeksi tidak dianalisis seperti yang dianalisis untuk virus, kami
memperkirakan bahwa tingkat tahunan infeksi pernapasan akut yang disebabkan oleh
rhinovirus adalah 3,5 per anak (95% CI, 3,3-3,6) pada anak-anak <2 tahun. infeksi
rhinovirus diperkirakan menyebabkan rata-rata
31 hari penyakit pernafasan per anak per tahun. Angka infeksi rhinovirus adalah
tertinggi di antara anak-anak 6 sampai 23 bulan usia, tapi terasa tinggi sudah
sebelum usia 6 bulan.

28 (68)
16 (39)
7 (17)
0 (0)
1 (2)
3 (7)
0 (0)
0 (0)
1 (2)
606 (69)
436 (49)
101 (11)
11 (1)
11 (1)
12 (1)
14 (2)
1 (0)
20 (2)
Values are n (%) for acute respiratory infections with a nasal swab specimen collected. HEV, human enterovirus; HMPV, human metapneumovirus; PIV, parainuenza virus.

5511(60)
(65)
335(36)
(29)
9 4(10)
(24)
0 0(0)
(0)
10 0(11)
(0)
1 0(1)
(0)
22(2)
(12)
0 0(0)
(0)
0 0(0)
(0)
Any virus
Rhinovirus
RSV
HEV
PIV 1, 2, or 3
Inuenza virus A or B
HMPV
Adenovirus
Coinfection

3340 (71) 130489


(76)(70)
2775 (59) 71351
(41)(50)
262 (6) 45 82
(26)
(12)
69 (1)
0 (0)
11 (2)
66 (1)
1 (1)
5 (1)
45 (1)
1 (1)
9 (1)
41 (1)
2 (1)
11 (2)
10 (0)
0 (0)
2 (0)
72 (2)
10 18
(6)(3)

1664 (66)
1201 (48)
239 (10)
30 (1)
61 (2)
38 (2)
31 (1)
7 (0)
57 (2)

Hospitalization
(n = 41)
Outpatient Ofce Antibiotic
Visits (n = 2503)Treatments
(n = 882)
Laryngitis
Pneumonia
(n(n= =92)
17)
Acute Respiratory
Wheezing
Acute Otitis
Illness
Media
Infection (n = 4728)
(n (n
= 172)
= 702)
Detected Virus

TABLE 2 Viruses Detected in Nasal Swabs During Acute Respiratory Infections and Associated Diagnoses and Outcomes

Complications, Antibiotic
Treatments, Day Care Absences, and
Hospitalizations
Acute otitis media was documented in 13% of all rhinoviruspositive acute respiratory infections (Table 3). Antibiotic treatment
was pre- scribed in 15% of cases, and use
of over-the-counter pain or fever medications was common.
Thirty- six percent of rhinovirus infections led to a physician visit,
and acute otitis media was diagnosed in 35% of rhinovirus
infections during which a child was evaluated by a physician. As a
result of the high frequency of rhinovirus infections, they
accounted for 50% of acute otitis media episodes, 41% of
wheezing illnesses, 49% of antibiotic treatments, and 48% of
physician visits during virologically studied acute respiratory
infections (Table 2). The estimated mean annual rate of rhinovirusassociated acute otitis media was 47 per 100 children (95% CI, 42
52) and 61 per 100 children (95% CI, 5568) for rhinovirusassociated antibiotic treatment (Table 4). Rhinovirus was associated
with 39% of hospitalizations for an acute respiratory infection
studied for viruses. The annual rate of
lower respiratory tract infections (wheezing or pneumonia)
associated with rhinovirus was estimated as 8.5 per 100 children
(95% CI, 6.311.1), and the rate of hospitalizations
as 2.3 per 100 children (95% CI,
1.23.9).
Over half (51%) of all children attending day care stayed at home
during rhinovirus infection, thereby also often necessitating
parental absenteeism from work. Of a total
of 856 lost work days for childrens acute respiratory infections,
439 (51%) were associated with rhinovirus infections. The
estimated rate of lost work days because
of a childs rhinovirus infections was 3.4 per child per year (95%
CI,
2.94.0) among children attending day care.

Seasonality
Rhinoviruses were detected throughout the year with high- incidence periods from August to November
and from April to May (Supplemental Fig 3). From August to November, rhinovirus was found in
73% of acute respiratory infections and in 68% of acute otitis media cases.

Rhinovirus Prevalence at 2, 13, and


24 Months of Age
The proportion of children positive for rhinovirus regardless of symptoms increased from 14% at 2
months of age to 18% at 13 months of age and to 24% at 24 months
of age (P < .001) (Fig 2). At the scheduled visits, 653 of 2316 children (28%) had respiratory symptoms
when specifically asked by the study nurse. Children with respiratory symptoms were more frequently
positive for rhinovirus (41%) than asymptomatic children (9%, P < .001) (Supplemental Table 6).
Comparing rhinovirus-positive and rhinovirus- negative asymptomatic children, symptoms occurring
from 14 days before to 7 days after sampling, and respiratory symptoms in other family members, were
more frequently documented in those who were rhinovirus-positive (Supplemental Table 7).
Asymptomatic rhinovirus- positive children had lower numbers of rhinovirus copies in nasal swabs (mean
[SD] log of copies per swab,
4.5 [1.3]) than did children with respiratory symptoms (mean [SD] log of copies per swab, 5.7 [1.5];
P < .001).

DISCUSSION
Rhinoviruses were detected in more than half of all acute respiratory infections in this birth cohort
study, resulting in an estimated annual
rate of 3.5 infections per child. Only a minority of the children were hospitalized but, still, the total
burden of illness caused by

TABLE 3 Characteristics of Acute Respiratory Infections Positive or Negative for Rhinovirus in Children 0 to 23 Months of Age
Characteristic

Symptoms

Acute Respiratory Infectionsa

Rhinovirus-Positive
(n = 2775)

Rhinovirus-Negative
(n = 1884)
Duration of respiratory symptoms, median [IQR], d
Rhinorrhea
Cough
Fever 38.0C
Wheezing reported by the parents or by the doctor
Poor appetite
Vomiting or diarrhea
Outcomes
Child absent from day careb
Duration of absenteeism from day care, median [IQR], d
Parent absent from work because of childs illnessb
Duration of absenteeism from work, median [IQR], d
Episodes with an outpatient clinic or emergency department visit
Hospitalization
Duration of hospitalization, median [IQR], d
Diagnosis (other than acute respiratory infection)
Acute otitis media
Wheezing illness (bronchiolitis or recurrent wheezing)
Laryngitis
Conjunctivitis
Pharyngitis or tonsillitis
Pneumonia
Treatment
Antibiotic treatment during episode
Systemic corticosteroids
Analgesics or antipyretics
Antitussives

9.0 [6.013.0]
2358/2383 (99.0)
1449/2383 (60.8)
715/2383 (30.0)
215/2391 (9.0)
495/2197 (22.5)
211/2197 (9.6)

9.0 [5.013.0]
1488/1623 (91.7)
1173/1623 (72.3)
784/1623 (48.3)
218/1627 (13.4)
409/1341 (30.5)
206/1341 (15.4)

.16
<.001
<.001
<.001
<.001
<.001
<.001

197/384 (51.3)
2.0 [1.04.0]
163/384 (42.4)
2.0 [1.03.0]
999 (36.0)
16 (0.6)
1.0 [1.02.0]

171/273 (62.6)
3.0 [2.04.0]
130/273 (47.6)
2.0 [1.04.0]
1030 (54.7)
24 (1.3)
1.0 [1.02.0]

.004
.14
.19
.26
<.001
.01
.39

351 (12.6)
71 (2.6)
33 (1.2)
48 (1.7)
33 (1.2)
5 (0.2)

333 (17.7)
91 (4.8)
59 (3.1)
43 (2.3)
48 (2.5)
12 (0.6)

<.001
<.001
<.001
.18
<.001
.01

408 (14.7)
20 (0.7)
992/2197 (45.2)
57/2197 (2.6)

392 (20.8)
18 (1.0)
749/1341 (55.9)
64/1341 (4.8)

<.001
.38
<.001
.001

Values are n (%) of acute respiratory infections unless otherwise specied. Two-sided P values calculated by MannWhitney U test for continuous variables and by 2 test for
categorical variables.
a
Episodes positive for rhinovirus and another virus were excluded from the analysis (n = 69). Of 1884 rhinovirus-negative acute respiratory infections, 262 were positive for RSV, 69
for human enterovirus, 66 for parainuenza virus type 1, 2 or 3, 41 for human metapneumovirus, 24 for inuenza virus A, 21 for inuenza virus B, 10 for adenovirus, 2 for RSV and
human metapneumovirus, 1 for human enterovirus and inuenza virus A, and 1388 were virus-negative.
b
Calculated among children attending outside-home day care at the time of acute respiratory
infection.

TABLE 4 Rates of Acute Respiratory Infections and Associated


Outcomes
Acute Respiratory
Infections

Rhinovirus-Positive Acute
Respiratory Infections

Proportion of Rhinovirus- Positive Infections from Infections Analyzed for Viruses

Estimated Rhinovirus-Associated
Acute Respiratory Infectionsa
At the age of 023 mo
At the age of 05 mo
At the age of 611 mo
At the age of 1223 mo
Days with respiratory symptoms
Outpatient ofce visits
Hospitalization
Additional diagnoses and
treatment
Acute otitis media
Wheezing
Pneumonia
Antibiotic treatment
a

Mean/Child/y (95% CI)


5.9 (5.76.1)
2.0 (1.92.1)
3.9 (3.74.2)
1.5 (1.31.6)
6.5 (6.26.7)
2.4 (2.22.6)
7.1 (6.87.3)
2.1 (1.92.2)
50.4 (47.853.2)
18.8 (17.120.6)
3.1 (2.93.3)
0.9 (0.80.9)
Mean/100 Children/y (95% CI)
5.9 (4.47.9)
1.2 (0.72.0)

94.2 (86.9102.3)
18.8 (15.423.0)
2.4 (1.83.4)
123.5 (114.2133.6)

24.9 (22.028.2)
5.1 (3.86.8)
0.4 (0.20.9)
30.8 (27.334.7)

Proportion (95% CI)


0.59 (0.570.60)
0.57 (0.540.60)
0.61 (0.580.63)
0.58 (0.560.60)
0.61 (0.600.61)
0.48 (0.460.50)
0.39 (0.250.55)

Mean/Child/y (95% CI)


3.5 (3.33.6)
2.3 (2.12.4)
3.9 (3.74.2)
4.1 (3.94.3)
30.7 (29.032.5)
1.5 (1.41.6)
Mean/100 Children/y (95% CI)
2.3 (1.23.9)

0.50 (0.460.54)
0.41 (0.340.49)
0.29 (0.130.54)
0.49 (0.460.53)

47.2 (41.952.2)
7.8 (5.810.2)
0.7 (0.21.3)
61.2 (54.568.2)

The number of rhinovirus-associated acute respiratory infections was determined as the product of the proportion of rhinovirus infections and total infections.

FIGURE 2

Prevalence of rhinovirus infections and respiratory symptoms in children at scheduled visits at the
age of 2, 13, and 24 months.

samples (Supplemental Table 5). Antibiotic prescriptions were even more frequent in infections without a
virologic analysis.
Rhinoviruses have been detected by sensitive PCR techniques in 11% to
47% of asymptomatic children.13,26
This has raised concern about the clinical relevance of rhinovirus- positive PCR results. In the current
study, the prevalence of rhinovirus was rather high at 2, 13, or 24 months of age, and it increased
with age. However, when parents were specifically asked about
respiratory symptoms in the child at the time of the visit, we found that rhinovirus was detected
infrequently in completely asymptomatic
children. Furthermore, rhinovirus detections were often associated
with respiratory symptoms before or after sampling, or with respiratory symptoms in other members of
rhinovirus was heavy. This disease
burden was particularly seen in the number of outpatient visits
for acute respiratory infections, of which almost half were associated with rhinovirus, and in the use
of antibiotics, with an annual rate of
61 antibiotic treatments per 100 children associated with rhinovirus infections. Even illness episodes
that do not require a physician visit may be burdensome for the families. We report that rhinovirus
infections associate with a high annual
number of days with respiratory symptoms, frequent over-the-counter medication use, and half of all
parental workdays lost because of a childs acute respiratory infection.
Our results show that, similar to earlier studies,2,24 acute otitis media complicates rhinovirus infections
more infrequently than respiratory infections caused by RSV or other viruses, but the high frequency of
rhinovirus infections makes it the most important virus associated with acute otitis media. We also
found
that 41% of acute wheezing illnesses

were associated with rhinovirus,


which is in line with a previous birth cohort study.13
By using home sampling in addition to sample collection at study
clinic visits, we were able to study viral etiology in 53% of all acute respiratory infections from birth to
2 years of age. Home nasal sampling for respiratory viruses has been found feasible previously, and it
may increase the virus detection rate in
studies of outpatient infections.19,20,25
Based on the frequency of complications and other outcomes in rhinovirus-positive versus rhinovirusnegative infections, we estimated rhinovirus-associated
morbidity. This estimation is subject to an assumption that rhinoviruses would occur with a similar
frequency and severity in infections that
were not analyzed for viruses as in those that were analyzed. This assumption is supported by the fact
that the rates of acute otitis media or pneumonia did not difer between infections with or without nasal

the family. Our results suggest that totally asymptomatic rhinovirus infections are less frequent than
previously thought, and incidental rhinovirus findings are often due
to infections with mild symptoms. This is in accordance with previous studies reporting high rates of
recurrent infections caused by diferent rhinovirus types, and limited duration of virus shedding in
immunocompetent individuals, rather than prolonged carriage
of the same rhinovirus type.2729
The prevalence of rhinovirus increased with age, as did the rates of respiratory infections, probably
because of increased contact with other children.
Viruses other than rhinovirus and coinfections by 2 viruses were found in a low proportion of
respiratory infections in this
study, which may be explained by our study setting and the virologic methodology. We investigated
mostly mild infections in a healthy child cohort. The influenza A/H1N1 pandemic in 2009 was mild in
this

population with high vaccination coverage against it, and the 4-year study period probably evened the
efects of epidemic variation in the circulation of respiratory viruses.
Our study has limitations. Because we focused on rhinovirus, our virologic methods were not
comprehensive for all other respiratory viruses. PCR was performed for rhinovirus, RSV, human
enterovirus, and influenza A and B viruses, whereas an antigen detection test, with lower sensitivity
than PCR, was performed for 5
other viruses. The sensitivity of the PCR may be better for rhinoviruses and enteroviruses than for RSV
in home-collected specimens because nonenveloped viruses endure the environment better than
enveloped viruses. Because consulting the study physician was easy and
free for the study families, the rate of physician visits might be overestimated. However, only about
half of all outpatient visits occurred at the study clinic, which was not

open during evenings and weekends. Our study children had older
siblings less frequently than those who did not participate. Because respiratory tract infections are
more frequent in children with large families, 17 this diference might have led to underestimation of the
impact of rhinovirus infections.

CONCLUSIONS
This study provides new information about the major role of rhinovirus
in acute respiratory infections in young children. Rhinovirus infections are generally mild, but because of
their high frequency, the impact of rhinovirus on the total number of acute otitis media and antibiotic
treatments in young children is greater than that of any other respiratory virus. In addition to
other benefits, novel prevention or treatment modalities of rhinovirus infections could substantially
decrease the use of antibiotics
for respiratory tract infections in children.

ACKNOWLEDGMENTS
We thank all of the families that participated in this study, the midwives for their help in recruiting the
families, and the whole STEPS Study team for assistance with
data collection, study nurses Niina Lukkarla, Petra Rajala, and Mira Katajamki for their assistance in
the study clinic, Tiina Ylinen for her technical assistance in virus detection, Anne Kaljonen for her
assistance with data handling, and
Jaakko Matomki for his assistance with statistical analyses.

ABBREVIATIONS
CI:confidence interval
IQR:interquartile range
PCR:polymerase chain reaction RSV:respiratory syncytial virus STEPS:Steps to the Healthy
Development and
Well-being of Children
FINANCIAL DISCLOSURE: The authors have indicated they have no nancial relationships relevant to this article to disclose.
FUNDING: All phases of this study were supported by the University of Turku; Abo Akademi University; Turku University Hospital; Academy of Finland grants
123571, 140251, and 277535; the Foundation for Pediatric Research; and research funds from Specied Government Transfers, Hospital District of Southwest
Finland. Dr Toivonen was also supported by Satakunta Hospital District, the National Graduate School of Clinical Investigation, the University of Turku
Graduate School, and the Paulo Foundation.
POTENTIAL CONFLICTS OF INTEREST: The authors have indicated they have no potential conicts of interest to disclose.

REFERENCES
1. Monto AS, Sullivan KM. Acute
respiratory illness in the community.
Frequency of illness and the
agents involved. Epidemiol Infect.
1993;110(1):145160
2. Chonmaitree T, Revai K, Grady JJ,
et al. Viral upper respiratory tract
infection and otitis media complication
in young children. Clin Infect Dis.
2008;46(6):815823
3. van der Zalm MM, Uiterwaal CS,
Wilbrink B, et al. Respiratory
pathogens in respiratory tract
illnesses during the rst year of life: a
birth cohort study. Pediatr Infect Dis J.
2009;28(6):472476

4. Miller EK, Lu X, Erdman DD, et al; New


Vaccine Surveillance Network.
Rhinovirus-associated hospitalizations
in young children. J Infect Dis.
2007;195(6):773781
5. Peltola V, Jartti T, Putto-Laurila A,
et al. Rhinovirus infections in
children: a retrospective and
prospective hospital-based study.
J Med Virol. 2009;81(10):
18311838
6. Iwane MK, Prill MM, Lu X, et al.
Human rhinovirus species
associated with hospitalizations
for acute respiratory illness in
young US children. J Infect Dis.
2011;204(11):17021710

7. Kieninger E, Fuchs O, Latzin P, Frey U,


Regamey N. Rhinovirus infections in
infancy and early childhood. Eur
Respir J. 2013;41(2):443452
8. Mackay IM, Lambert SB, Faux CE, et al.
Community-wide, contemporaneous
circulation of a broad spectrum of
human rhinoviruses in healthy
Australian preschool-aged children
during a 12-month period. J Infect Dis.
2013;207(9):14331441
9. Jacobs SE, Lamson DM, St George K,
Walsh TJ. Human rhinoviruses. Clin
Microbiol Rev. 2013;26(1):135162
10. Blomqvist S, Roivainen M, Puhakka
T, Kleemola M, Hovi T. Virological and

serological analysis of rhinovirus infections during the rst two years of life in a cohort of children. J Med Virol.
2002;66(2):263268
11. Fox JP, Cooney MK, Hall CE. The Seattle virus watch. V. Epidemiologic observations of rhinovirus
infections, 1965-1969, in families
with young children. Am J Epidemiol.
1975;101(2):122143
12. Monto AS, Bryan ER, Ohmit S.
Rhinovirus infections in Tecumseh, Michigan: frequency of illness and number of serotypes. J Infect Dis.
1987;156(1):4349
13. Kusel MM, de Klerk NH, Holt PG, Kebadze T, Johnston SL, Sly PD. Role of respiratory viruses in acute upper and lower respiratory tract
illness in the rst year of life: a birth cohort study. Pediatr Infect Dis J. 2006;25(8):
680686
14. Regamey N, Kaiser L, Roiha HL,
et al; Swiss Paediatric Respiratory Research Group. Viral etiology of acute respiratory infections with cough in infancy: a community-based
birth cohort study. Pediatr Infect Dis J.
2008;27(2):100105
15. Fairchok MP, Martin ET, Chambers S,
et al. Epidemiology of viral respiratory tract infections in a prospective cohort of infants and toddlers attending daycare. J Clin Virol.
2010;49(1):
1620
16. Budge PJ, Grifn MR, Edwards KM, et al; RESPIRA-PERU Group. A household- based study of acute viral respiratory illnesses in Andean
children. Pediatr Infect Dis J. 2014;33(5):443447

17. Anders KL, Nguyen HL, Nguyen NM, et al. Epidemiology and virology of acute respiratory infections during the rst year of life: a birth cohort
study in Vietnam. Pediatr Infect Dis J.
2015;34(4):361370
18. Lagstrm H, Rautava P, Kaljonen A, et al.
Cohort prole: Steps to the healthy development and well-being of children (the STEPS Study). Int J Epidemiol.
2013;42(5):12731284
19. Peltola V, Waris M, sterback R, Susi P, Ruuskanen O, Hyypi T. Rhinovirus transmission within families with children: incidence of
symptomatic and asymptomatic infections. J Infect Dis. 2008;197(3):382389
20. Waris M, sterback R, Lahti E, Vuorinen T, Ruuskanen O, Peltola V. Comparison of sampling methods for the detection of human rhinovirus
RNA. J Clin Virol.
2013;58(1):200204
21. sterback R, Tevaluoto T, Ylinen T, et al. Simultaneous detection and differentiation of human rhino- and enteroviruses in clinical specimens
by real-time PCR with locked nucleic Acid probes. J Clin Microbiol.
2013;51(12):39603967
22. Toivonen L, Schuez-Havupalo L, Rulli M, et al. Blood MxA protein as a marker for respiratory virus infections
in young children. J Clin Virol.
2015;62:813
23. Jokela P, Vuorinen T, Waris M, Manninen R. Performance of the Alere i inuenza A&B assay and
mariPOC test for the rapid detection of inuenza A and B viruses. J Clin Virol.
2015;70:7276

24. Vesa S, Kleemola M, Blomqvist S, Takala A, Kilpi T, Hovi T. Epidemiology of documented viral respiratory infections and acute otitis media in
a cohort of children followed from two to twenty-four months of age. Pediatr Infect Dis J. 2001;20(6):574581
25. Lambert SB, Allen KM, Druce JD, et al.
Community epidemiology of human metapneumovirus, human coronavirus NL63, and other respiratory viruses
in healthy preschool-aged children using parent-collected specimens. Pediatrics. 2007;120(4). Available at:
www.pediatrics.org/cgi/content/full/
120/4/e929
26. Jartti T, Jartti L, Peltola V, Waris M, Ruuskanen O. Identication of respiratory viruses in asymptomatic subjects: asymptomatic
respiratory viral infections. Pediatr Infect Dis J.
2008;27(12):11031107
27. Jartti T, Lee WM, Pappas T, Evans M, Lemanske RF Jr, Gern JE. Serial viral infections in infants with recurrent respiratory illnesses. Eur
Respir J.
2008;32(2):314320
28. van der Zalm MM, Wilbrink B, van Ewijk BE, Overduin P, Wolfs TF, van der Ent CK. Highly frequent infections with human rhinovirus in healthy
young children: a longitudinal cohort study. J Clin Virol.
2011;52(4):317320
29. Peltola V, Waris M, Kainulainen L, Kero J, Ruuskanen O. Virus shedding after human rhinovirus infection in children, adults and patients with
hypogammaglobulinaemia. Clin Microbiol Infect. 2013;19(7):E322E327

Rhinovirus Infections in the First 2 Years of Life


Laura Toivonen, Linnea Schuez-Havupalo, Sinikka Karppinen, Tamara
Teros-Jaakkola, Maris Rulli, Jussi Mertsola, Matti Waris and Ville Peltola
Pediatrics 2016;138;; originally published online August 31, 2016;
DOI: 10.1542/peds.2016-1309
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PEDIATRICS is the official journal of the American Academy of Pediatrics. A monthly


publication, it has been published continuously since 1948. PEDIATRICS is owned, published,
and trademarked by the American Academy of Pediatrics, 141 Northwest Point Boulevard, Elk
Grove Village, Illinois, 60007. Copyright 2016 by the American Academy of Pediatrics. All
rights reserved. Print ISSN: 0031-4005. Online ISSN: 1098-4275.

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Rhinovirus Infections in the First 2 Years of Life


Laura Toivonen, Linnea Schuez-Havupalo, Sinikka Karppinen, Tamara
Teros-Jaakkola, Maris Rulli, Jussi Mertsola, Matti Waris and Ville Peltola
Pediatrics 2016;138;; originally published online August 31, 2016;
DOI: 10.1542/peds.2016-1309

The online version of this article, along with updated information and services, is
located on the World Wide Web at:
/content/138/3/e20161309.full.html

PEDIATRICS is the official journal of the American Academy of Pediatrics. A monthly


publication, it has been published continuously since 1948. PEDIATRICS is owned,
published, and trademarked by the American Academy of Pediatrics, 141 Northwest Point
Boulevard, Elk Grove Village, Illinois, 60007. Copyright 2016 by the American Academy
of Pediatrics. All rights reserved. Print ISSN: 0031-4005. Online ISSN: 1098-4275.

Downloaded from by guest on September 23, 2016

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