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INTRODUCTION
red
(Anief,
blood
cells.
Corresponding
chemical
2000).................................................
and
hydrophobic
distinct
binding
sites
(Syukri,
2002)..................................................
Giving
intravenous
tetracycline
(drug
free)
(Chein,
1987).
work,
length
of
employment
and
the
Procedures
mL.
RESEARCH METHODS
2. Preparation of Solutions
on
Laboratory
of
Biofarmaceutics
Pharmacokinetics
Sriwijaya
University
1.00-5.00 p.m.
3. Measurement Absorbance
Standard
(Shimadzu
magnetic
dynamic
solution
absorbance
UV-Vis
spectrophotometer
and
EB,
Japan),
dialysis
setup,
stirrer,
distilled
water
until
clean.
Observations
eggshell
30,
spectrophotometer
there
the
cumulative
is
were
water
made
on
the
using
outside
of
60,
90
minutes
(
360
using
nm).
UV-Vis
Repeat
DATA ANALYSIS
1. Standard Absorbances
Concentratio
ns
0,2
0,4
0,6
0,8
1
1,5
Absorbances
A
B
0,15
0,161
0,162
0,172
0,238
0,298
0,151
0,162
0,167
0,175
0,207
0,343
Curve B
0.4
absorbance
0.4
absorbancei
0 0.5 1 1.5 2
concentration
0 0.5 1 1.5 2
concentration
2. Absorbance tetracycline
Times
5
10
15
30
60
90
Absorbances
A
0,368
0,362
0,318
0,253
0,211
0,202
B
0,378
0,375
0,327
0,223
0,205
0,201
curve A
0.4
0.4
f(x) = - 0x + 0.36
0.2 R = 0.84
absorbnce
absorbance
f(x) = - 0x + 0.36
0.2 R = 0.74
0
50
100
time
50
100
time
Absorbances
A
B
0,211
0,201
0,279
0,273
0,298
0,32
0,349
0,405
0,399
0,423
0,447
0,441
curve B
0.6
0.6
0.4
f(x) = 0x + 0.25
R = 0.9
absorbance 0.2
0.4
f(x) = 0x + 0.26
R = 0.72
absorbace 0.2
0
0
50
100
50
time
100
time
Absorbances
A
B
0,246
0,237
0,297
0,281
0,354
0,339
0,386
0,415
0,428
0,43
0,463
0,456
5
10
15
30
60
90
curve A
curve B
0.6
0.5
0.4
f(x) = 0x + 0.29
R = 0.83
absorbance 0.2
0
f(x) = 0x + 0.28
R = 0.76
absorbance
0
50
time
100
50
100
time
Amount of
Cumulative
Diffused
Percent
Drug (mg)
(%)
5
10
15
30
60
90
0,1097
0,1071
0,0887
0,0613
0,0437
0,0399
10,97
21,68
30,55
36,68
41,04
45,03
Group B
5
10
15
30
60
90
Amount of Diffused
Cumulative Percent
Drug (mg)
(%)
0,1
0,0989
0,082
0,0454
0,0391
0,0377
10
19,89
28,09
32,63
36,54
40,31
spreads
release.
percent
cumulative
drug
Amount of
Cumulative
Diffused
Percent
Drug (mg)
(%)
5
10
15
30
60
90
0,0437
0,0723
0,0803
0,1017
0,1227
0,1429
4,37
11,59
19,62
29,79
42,06
56,35
Group B
5
10
15
30
60
90
Amount of Diffused
Cumulative Percent
Drug (mg)
(%)
0,0151
0,0258
0,0318
0,0438
0,0463
0,0488
1,51
4,09
7,27
11,65
16,28
21,16
spreads
release.
cumulative
into the
percent
cumulative
drug
percent
of
drug
release
by
to proteins.
cumulative_percent
Statistic
df
Shapiro-Wilk
Sig.
Statistic
df
Sig.
blue a
.191
.200*
.847
.148
blue b
.312
.069
.767
.059
blue a
.172
.200*
.950
.738
blue b
.289
.129
.885
.291
cumulative_percent
Statistic
df
Shapiro-Wilk
Sig.
Statistic
df
Sig.
blue a
.127
.200*
.990
.988
blue b
.238
.200*
.945
.700
blue a
.153
.200*
.968
.876
blue b
.158
.200*
.964
.848
c. 1 mL Tetracycline + 1 mL Plasma
cumulative_percent
Statistic
df
Shapiro-Wilk
Sig.
Statistic
df
Sig.
blue a
.156
.200*
.981
.955
blue b
.267
.200*
.904
.398
blue a
.142
.200*
.970
.890
blue b
.192
.200*
.971
.901
distributed.
Correlations
cumulative_perc Ammount_of_diff
Group
Group
Pearson Correlation
ent
1
-.111
-.165
.540
.607
12
12
12
Pearson Correlation
-.011
.963**
Sig. (2-tailed)
.730
Sig. (2-tailed)
N
cumulative_percent
used_drug
N
Ammount_of_diffused_dru Pearson Correlation
.000
12
12
12
-.155
.909**
.607
.000
12
12
g
Sig. (2-tailed)
N
12
8. T-Test Analysis
One-Sample Test
t-test for equality of means
Df
amount_of_drug_diffused
blue a
blue b
cumulative_percent
blue a
blue b
Sig. (2-tailed
6
.000
.000
.000
.000
Mean difference
1.00375
1.009
50.0100
50.0100
CONCLUSION
A and group B.
REFERENCES
Pengembangan
Bentuk
Biofarmasetika &
Surabaya.