TM

PERFORMANCE MATERIALS

Reagents for Mindray analyzers

Mindray BC-5500, BC-5200
tWBC + 5 part differential analysis
Reagent

Description

Product Nr.

Pack size

Diluent

Diluid M5

3419.9020PC

20L

Lysing reagent

CyMet MH CN Free

3497.0500PE

500 mL

Baso reagent

CyMet MBA

3489.1000PE

1L

Diff reagent

CyMet MD(I)

3418.1000PE

1L

Diff reagent

CyMet MD(II)

3488.0500PE

500 ml

Cleaner

ProClean MX5

3498.1000PE

1L

Hematology Control

BC Diff 5 Control L/N/H

3613, 3614, 3615

3.0 ml

The connection of the 20L polycube can be optimised by using our adaptor (code 82166).

Mindray BC-5300
tWBC + 5 part differential analysis
Application
Diluent
Lysing reagent

Cleaner
Hematology Control

Description
Diluid M5
CyMet MD(I) 53
CyMet MD(II)
CyMet LH 53
Proclean MX5
BC Diff 5 Control L/N/H

Product Nr.
3419.9020PC
2984.1000PE
3488.0500PE
2985.1000PE
3498.1000PE
3613/3614/3615

Pack size
20 L
1L
500 ml
1L
5L
3.0 ml

Product Nr.
3439.9020PC
3441.0500PE
3440.0500PE
3442.5000PE
3901
3917
3917.5000
3427/3428/3429
3463/3464/3465
3701/3702/3703
3433/3434/3435
3820/3821/3822

Pack size
20 L
500 ml
500 ml
5L
100 ml
1L
5L
2.5 ml
2.5 ml, pierceable
4.5 ml
2.5 ml
4.5 ml

Mindray BC-2800, BC-3000 Plus, BC-3200, Automated

tWBC + 3 part differential analysis
Application
Diluent
Lysing reagent
Rinsing/Auxiliaries
Emergency Cleaning

64

Description
Diluid Mindray
CyMet Mindray
CyMet Mindray CN Free
Rinse Mindray
ProClean Plus
Hypochlorite 0.5%

Hematology Control

8 Parameter Control L/N/H

Hematology Control

3 Diff Control L/N/H

Medonic

TM

melet schloesing

PERFORMANCE MATERIALS

Mindray BC-2000, BC-2300, Semi-Automated

tWBC + 3 part differential analysis

Emergency Cleaning

ProClean Plus
Hypochlorite 0.5%

Hematology Control

8 Parameter Control L/N/H

Hematology Control

3 Diff Control L/N/H

Product Nr.
3439.9020PC
3771
3441.0500PE
3862.1000
3862.5000
3901
3917
3917.5000
3427/3428/3429
3463/3464/3465
3701/3702/3703
3433/3434/3435
3820/3821/3822

Pack size
20 L
10 x 10 ml
500 ml for BC 2300
1L
5L
100 ml
1L
5L
2.5 ml
2.5 ml, pierceable
4.5 ml
2.5 ml
4.5 ml

mindray

Cleaning

Description
Diluid Mindray
Lyzerglobin PCE
CyMet Mindray
ProClean Extra

nihon kohden

Application
Diluent
Lysing reagent

orphee

Diluid, CyMet and ProClean are all trademarks of Avantor Performance Materials Inc.

J.T.Baker

JTB Nr.

A12-000167

Diluid M5

3419.9020PC

M-50 LHLysing reagent

A12-000221

CyMet MH CN Free

3497.0500PE

M-50 LBA Baso reagent

A12-000224

CyMet MBA

3489.1000PE

M-50 LEO (I) Diff reagent

A12-000219

CyMet MD(I)

3418.1000PE

M-50 LEO (II) Diff reagent

A12-000220

CyMet MD(II)

3488.0500PE

M-50 Cleanser

A12-000225

ProClean MX5

3498.1000PE

swelab

H. Nr

M-50D Diluent

sysmex

Mindray BC-5500/5200

urit

Diluent
Diluent
Rinse
Rinse
E-Z Cleaner
Probe cleanser

controls

JTB Nr.
3441.0500PE
3440.0500PE
3439.9020PC
3439.9020PC
3442.5000PE
3442.5000PE
3901
3917

cleaners

A12-000042 5.5L
A12-000047 20L
A12-000043 5.5L
A12-000048 20L
A12-000045100 ml
A12-00004617 ml

J.T.Baker
CyMet Mindray
CyMet Mindray CN Free
Diluid Mindray
Diluid Mindray
Rinse Mindray
Rinse Mindray
ProClean Plus
Hypochlorite

pbs

H Nr.
A12-000044 500 ml

Stains

Mindray BC-3000
Lyse

seac

Cross list: Mindray reagents vs. J.T.Baker

65

TM

PERFORMANCE MATERIALS

Priming and Calibration of Mindray Analyzers

Mindray BC-3000 Plus
Before switching from original reagents to Avantor Performance
Materials reagents it is important to know that the instrument is
running without any problems and that the values measured for
the blood controls (all three levels) are within the specifications
of the assay value sheet. Therefore it is necessary to run blood
controls or some fresh reference patient samples before you
switch to our reagents. These values are necessary for any
calibration afterwards.
Priming of Mindray BC-3000plus
1. Choose the option <SERVICE> from the main menu of the
software program by using the cursor keys. Then choose
<MAINTENANCE> and press <ENTER>.


Remove the aspirating tube from the Mindray reagent
cubitainer which you want to replace. Dont lay the tubes
on tissues or dusty surfaces. Place them in clean beakers.
Reagent alarms will occur!

It is important to rinse the outside surface of the aspirating
tubes with distilled or de-ionised water to prevent any kind
of reagent contamination.
2. Choose the functional key <PRIME> followed by <ENTER>
from the reagent you want to replace:

Mindray
Avantor

M-30D Diluent =
Diluid Mindray

M-30R Rinse =
Rinse Mindray

M-30L Lyse = CyMet Mindray or CyMet Mindray
CN Free

For example press <DILUENT PRIME>, <ENTER> when you

want to replace the M-30D Diluent for Diluid Mindray.
Hold the reagent sensor in a way (move sensor up) that the
reagent alarm will stop and that you can activate a reagent
prime with air.

INFORMATION IN THIS DISTRIBUTOR CATALOGUE. ALSO REFER

TO SECTION 5 OF THE BC-3000PLUS OPERATORS MANUAL.
1. Run blood controls or the same fresh patient samples that
you ran before changing the original reagents for Avantor
reagents.
2. Choose the option <CALIBRATION> from the MAIN menu.
Then choose <MANUAL CALIBRATION> and press <ENTER>
to access Manual Calibration screen. The calibration factors
are shown in a table.
3. Calculate the New Calibration Factors. Use the following
formula to calculate the new calibration factors:

old factor x reference value

New factor = --------------------------------------------Average of test value

See EXAMPLE at the bottom of the page.

4. Enter the new Calibration Factors for all the parameters you
want to calibrate.
5. Confirm the new Calibration Factors.
6. After entering new factors, run the controls or patient
samples again. Verify the results.
Example
5 fresh patient samples were measured with Mindray
reagents. Next MCV values are measured: 91, 88, 89, 87, 89.
The mean value for MCV is 89.
After priming the Avantor reagents into the instrument the
same 5 fresh patient samples gave next MCV values: 86, 84,
87, 84, 86. The mean value of MCV is 86.
Old calibration factor in the whole blood mode is 97,2%.

3. Place the aspiration tube in the cubitainer with Avantor

reagent. Choose the functional key <PRIME> followed by
<ENTER> from the reagent you want to replace:

REPEAT STEPS 1 TO 3 FOR ALL THE THREE REAGENTS

CONNECTED!!

4. Choose the functional key <EMPTY BATHS>, <ENTER> to

empty the diluent from the WBC and RBC baths. After that,
press <ENTER> to prime the Diluent into the baths
5. Perform at least 5 blank counts and check the background
values. Acceptable values for the background are:

WBC
< 0.5 K/l
HGB
< 0.2 g/dL

RBC
< 0.05 M/l
PLT
< 10 K/l

Repeat background counts until acceptable values are
found.
Manual calibration of Mindray BC-3000Plus Analyzers
FOR CALIBRATION INSTRUCTIONS AND CALCULATING NEW
CALIBRATION FACTORS REFER TO THE PARAGRAPH GENERAL

66

old factor x reference value

New factor = ----------------------------------------------------Average of test value
97,2 x 89
= ------------------ = 100,6 %
86

Note: For Automatic Calibration please refer to Section 5.4 in

the BC-3000plus Operators Manual.

Medonic

TM

melet schloesing

PERFORMANCE MATERIALS

Mindray BC-5500

Reagents for the Mindray BC5500 are bottled in similar

square bottles as the original reagents. For the Diluid
cubitainer we will provide our customers a special
adapter to overcome the difference in height due to the
fixed Diluent reagent aspiration tube.

T o guarantee optimal performance of the JT Baker

reagents, the priming protocol mentioned in this
handout should be followed every time you switch from
original Mindray reagents to JT Baker reagents or vice
versa. Especially the priming protocol for the Diluent and
the CyMet MD(I) reagents should be performed correctly
to avoid cross contamination between the reagents.

Before changing any reagents on the Mindray BC5500

instrument, run at least 5-10 fresh human samples or
BC-Diff 5 control blood with the original Mindray
reagents for reference and calibration purposes.

J .T. Baker differential reagents result in a different

separation between the mononuclear (Lymphocytes
and Monocytes) and polynuclear (Neutrophils,
Eosinophils and Basophils) cells compared to Mindray
reagents. Figure 1 shows and example of the different
mononuclear and polynuclear white blood cells

mindray

J.T. BAKER

MINDRAY
Figure 2. Improved Monocytes / Neutrophils separation
J.T. Baker vs. Mindray

seac


Therefore we advise to set the Sensitivity for this
Abn./Atypical Lym flag to 4 instead of 5. Go to
SETUP, Advanced, Flag Sensitivity, Edit, Abn./
Atypical Lym and change the value to 4.

swelab

This current reagent system has been tested on BC5500

with software version up to 1.13. In case of any newer
software versions or any hardware modification of the
BC5500 model we cannot guarantee the performance of
the reagent.

J T Baker CyMet MBA reagent causes an effect on the

position of the mononuclear and polynuclear white
blood cells and Basophil population in the BASOscattergraph (see figure 3). The Mindray Basophil reagent
is much stronger than the JT Baker Basophil reagent
resulting in different gain settings for both reagent
packages. Please follow the Optical System Setup
procedure for Basophils mentioned in this handout.

sysmex

Figure 3. Basophil subpopulation J.T. Baker vs. Mindray

urit

T he recipe for the CyMet MD(I) has been improved on

Eosinophilia detection. Therefore these reagent
replacement instructions are intended for use with
CyMet MD(I) lots higher than lot 0928900030 and all lots
of CyMet MBA

controls

orphee

Please read these notes prior to installation of the

J.T.Baker reagent package on the Mindray BC5500 Auto
Hematology analyzer.

T he separation between Monocytes and Neutrophils

(see figure 2) is improved when using the JT Baker
reagents in comparison to the Mindray reagents. The
Monocytes are positioned closer to the Lymphocyte
area. Mindray reagents tend to give more often
Monocytosis flagging, where JT baker reagents tend to
give more often Abn./Atypical Lym? The Lymphocyte
cluster is more stretched with JT Baker reagents, but the
total amount counted is equal.

nihon kohden

1. Important NOTES

2. Priming protocol

Diluid M5

M-50LEO(I) Lyse

CyMet MD(I)

M-50LEO(II) Lyse

CyMet MD(II)

M-50LH Lyse

CyMet MH CN Free

M-50LBA Lyse

CyMet MBA

M-50 Cleanser

Proclean MX5

M-50P Probe Cleanser

Hypochlorite 0.5%

pbs

Figure 1. White blood cells subpopulations

4-part Diff Scattergraph

J.T. BAKER

M-50D Diluent

Stains

MINDRAY

cleaners

Use next cross list for correct reagent installation and usage.

67

TM

PERFORMANCE MATERIALS

For the complete Deprime of the instrument we advise to

use the Pack-up function from the Overall maintenance
screen. This is the best method to flush out the original
reagents, flush the system with water and prime the system
with JT Baker reagents.
Pack-up protocol (see page 10-33 operator manual)
Click the service icon in the Main screen and click the
Overall button to enter the maintenance screen. Proceed as
follows:
1. Click the Pack-up button at the Overall screen.
2. Click Yes to proceed with the pack-up.
3. Remove all reagent pickup tube assemblies from their
bottles and containers when prompted on the screen.
4. Click OK to start the fluidic system emptying.
5. A progress bar pops up and shows the remaining time.
6. When prompted place all reagent pickup assemblies
into a container with distilled water.
7. Click OK to start to flush the analyzer with distilled
water.
8. After this flushing process a dialog box will ask to
remove all reagent pickup assemblies from the distilled
water.
9. Remove them and place them in a clean beaker.
10. Click OK to start to empty the fluidic system.
11. The instrument will power off automatically.
12. Power on the instrument and wait for initialization to
finish.
13. Several alarms will sound.
14. Click the Count button and click on the upper alarm
box.
15. Place all reagent pickup assemblies in their corresponding J.T. baker reagents.
16. Click on the Remove error button and reagent priming
will start for all the reagents.
17. When ready, perform at least 5 background counts.
18. Perform the Optical System setup protocol.
19. After that perform the calibration of the instrument.

MAS = High angle scatter (horizontal X-axis), related to

complexity
LAS = Low angle scatter (vertical Y-axis), related to size
7m standard particle test (DIFF & BASO channel):
:
1) In the count screen select Mode and change to the
OV-PD mode and click Diluent to dispense diluent
into an empty tube 3 times.
2) Dispense 1 drop of 7m standard particle into the
centrifugal tube and mix them.
3) Change the mode to OV-Whole blood-CBC+5DIFF and
test the dilution as whole blood
4) Then go to the Review > OpAdjust screen and select
the DIFF channel (see figure 5).

Figure 4. Optical Adjust screen (OpAdjust)5)

5) Click Calculate to check the four parameters:
Mindray reagents: AS-peak, 88 4; LAS-peak, 45 4
JT Baker reagents: MAS-peak, 88 4; LAS-peak, 45 4

3. Optical System Setup

Next methods should be used setting up the new types of

Optical systems of the BC5500 instruments with JT Baker
reagents correctly:
1. Using standard latex particles of 7m.
2. Using fresh human samples.
Both methods are perfectly described in Mindray instruction
materials.
3.1. I nstruction on using the standard particles of 7m .
For the new optical system, 7m standard particle is used for
both channels (DIFF & BASO).
For the DIFF channel there are no differences for the
MAS-peak and LAS-peak between the Mindray and JT
Baker reagents.
For the BASO channel there are differences between the
Mindray and JT Baker reagents. MAS-peaks and
LAS-peaks are different.

68

MAS 0.1max width, =14

LAS 0.1max width, =14
6) Select the BASO channel and click Calculate to check
the two parameters:
Mindray reagents: MAS-peak, 147 4; LAS-peak, 58 4

JT Baker reagents: MAS-peak, 89 4; LAS-peak, 45 4
7) If the MAS/LAS-peak value is out of normal range, input
the target value and press >>> to calculate the new
Gain. Please write down the new Gain; then access
Setup > Gain screen to modify the gains accordingly.

Medonic

TM

WBC LAS DIFF J.T. Baker

WBC MAS DIFF J.T. Baker

= WBC LAS DIFF Mindray

= WBC MAS DIFF Mindray

WBC LAS BASO J.T. Baker

WBC MAS BASO J.T. Baker

= WBC LAS BASO Mindray -71

= WBC MAS BASO Mindray -66

mindray

Quick Optical Gain Setup method:

There are several important checks to see if the MAS-peak and

LAS-peak gains for the BASO are set up correctly:
There should be a clear separation between the Basophils
and the rest of the white blood cells.
If the bottom of the total WBC population is cut by a
straight line, the total WBC count is not ok and the LAS
PEAK gain has to be increased.
See figure 7 for an incorrect separation between Basophils
and other White Blood Cell populations. MAS PEAK gain
has to be lowered.

nihon kohden

3.2. Instructions using fresh samples

IIt is advised to setup the instrument using standard
latex particles. are not available, gains can be adjusted for
the DIFF and BASO channels according to experience.
Experienced customers can used next quick setup method
to adjust the optical gains for DIFF and BASO channels.

melet schloesing

PERFORMANCE MATERIALS

Mindray

J.T. Baker

WBC LAS DIFF

97

97

WBC MAS DIFF

70

70

WBC LAS BASO

161

90 (161 - 70)

WBC MAS BASO

191

125 (191 - 66)

Figure 7. Incorrect separation Basophil population

sysmex

swelab

After correct Optical Gain Setup a normal scatter graph for

the 4-part Differential with JT Baker reagents is shown in
figure 5.

seac

Gain

orphee

Example:

Figure 5. WBC 4-part Differential

controls

urit

There are several important checks to see if the MAS-peak and

LAS-peak gains for the DIFF are set up correctly:
There should be a clear separation between Lymphocytes
and Monocytes populations.
There should be a clear separation between
Neutrophils+Baso and Eosinophils populations.
The DIFF scattergraph should be 3/4 or 4/5 of the DIFF
screen (LAS-peak).
The centre of the Neu+Baso should be in the right middle
of DIFF scattergraph. Otherwise there will be too many left
shift and immature cells flags.

pbs

cleaners

A normal scatter graph for the Basophils with J.T. Baker reagents
is shown in figure 6.

Stains

Figure 6. Basophil population

69

TM

PERFORMANCE MATERIALS

4. Calibration

It is recommended before changing to JT Baker reagents to

run fresh human samples or the BC-Diff 5 control blood as
reference.
After installation of JT Baker reagents on the Mindray BC5500
instrument calibration is necessary for several CBC parameters. Especially HGB and MCV are affected by the change of
reagents.
Please refer to Chapter 9 of the Operation Manual how to use
the calibration programs. As well you can use the Quick
Calibration guide mentioned below.
.
Quick Calibration guide
If you ran fresh human samples with original Mindray
reagents before changing to JT Baker reagents you can use
next quick calibration guide to perform the calibration.
Step 1: Calculate the mean for parameters WBC, RBC, HGB,
MCV and PLT from the samples that were
measured with original Mindray reagents
(reference values)
Step 2:

 easure the same samples, but now with JT Baker

M
reagents

Step 3: Calculate the mean for parameters WBC, RBC, HGB,

MCV and PLT from the samples that were now
measured with JT Baker reagents
Step 4: At the Main menu, click the Calibration icon to
enter the Calibration screen. Print the old
calibration factors
Step 5: Calculate for every parameter the new calibration
factor
For example: MCVmindray = 92, MCVJ.T.Baker = 88 and
the old calibration factor is 99.0

New Cal factor for MCV =

New Cal factor for MCV =

MCVmindray
------------------ * Old factor MCV =
MCVJ.T.Baker
92
---- * 99.0 = 103.5
88

Step 6: Enter the new factors in the Calibration

screen
Step 7: For verification measure the same samples
again and compare the averages with the
reference values
Step 8:

70

If values are still not ok, repeat Step 5 to 7

About Avantor Performance Materials

Avantor Performance Materials manufactures and markets high-performance chemistries and materials
around the world under several respected brand names, including the J.T.Baker, Macron Fine Chemicals,
Rankem, Diagnova, BeneSphera, and POCH brands.
Avantor products are used in a wide range of industries. Our biomedical and life science solutions are
used in academic, industry and quality control laboratories for research, pharmaceutical production and
medical lab testing, while our electronics solutions are used in the manufacturing of semiconductors and
flat panel displays. Based in Center Valley, Pennsylvania (USA), Avantor is owned by an affiliate of New
Mountain Capital, LLC.
For additional information please visit www.avantormaterials.com or follow www.twitter.com/avantor_news

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