Stem Cell Revolution

Introducing Indias first
Sepax
Automated Processing Technology
1800 345 6200 (Toll free) or +91 98301 66200 | www.cordlifeindia.com | info@Cordlifeindia.com
TAKING THE FIRST
STEP
Dear Expectant Parent,

Congratulations! This must be an exciting time for you as you await the arrival of your
baby. With your pregnancy coming to a close, we are glad that you consider stem cell
banking as one of the essential preparations for your newborn.
Rich in stem cells, cord blood could one day save the lives of your loved ones. A precious
gift that would otherwise be discarded, cord blood derived stem cells are proven source of
treatment for life-threatening diseases such as leukemia and lymphoma. Researchers
worldwide are also studying the potential application of different types of stem cells
derived from another source your baby's umbilical cord. So far, studies reported have
been promising, giving families more reasons to store umbilical cord stem cells too for
future use.
We understand that stem cell banking is a personal decision that should be made with a
full understanding of the current and potential lifesaving possibilities. In the
following pages, we have put together accurate and
evidence-based information on cord blood and
umbilical cord stem cells. We hope this will help you
make an informed decision on this once-in-alifetime opportunity.
Just like the parent and child bond, we at
Cordlife, are with you for the long haul. Having
been entrusted by tens of thousands of
families across the Asia Pacific region,
we are confident that we have the
right expertise and experience to
help you take care of your baby's
stem cells. At the end of the day,
we are here to give hope and save
lives.
We look forward to your joining the
Cordlife community.
With you always,
Cordlife
www.cordlifeindia.com | 1
What is cord blood?

Cord blood, also called 'placental blood', is blood that remains in the umbilical cord and placenta following
the birth of a baby and after the umbilical cord is cut. During pregnancy, the umbilical cord functions as a
lifeline between mother and child. After the baby's delivery, the blood present in the umbilical cord could
offer hope for the child and members of the family.
What are cord blood stem cells?

Cord blood is a rich source of haematopoietic like stem cells (HSCs), a proven resource for the treatment of
many life-threatening diseases such as leukaemia and lymphoma. HSCs though are primarily responsible
for replenishing blood and regenerating the immune system, they also have the unique ability to differentiate
into various cell types found in the human body (as depicted in the diagram below).
Other types of cells :
Blood and Immune cells :
What is cord blood &

why should we bank it?
2 | www.cordlifeindia.com
Red blood cells
White blood cells
Platelets
Heart cells
Nerve cells
Liver cells
Advantages of cord blood stem cells

Although stem cells can also be found in bone marrow
and peripheral blood (blood that circulates through your
body), stem cells from cord blood are increasingly being
used as a preferred source of adult stem cells for
transplantation (hematopoietic reconstitution). The table
below illustrates the advantages cord blood/cord tissue
has over other sources of stem cells.
Source
Peripheral Blood
Bone marrow
Donor needs to be injected multiple

times with growth factors to induce
the release of HSCs into blood
stream which is collected in multiple
sittings by an extraction instrument
(Haema-cytapheresis).
Cord Blood/
Cord Tissue
At the time of delivery.

Quick, painless and
risk-free for both
mother and child.
Collection
Multiple extractions
from donor invasive
operation under general
anesthesia.
Availability
Requires extensive donor Search.
Readily available
when needed.
Quality of
stem cells
Not as potent.
Younger and more

primitive.
Higher chance of complications after infusion.
Lower risk of
complications
Requires a perfect match between donor and recipient
Matching is easier
Can cost as high as USD30,000*
100% ownership with

no extra retrieval cost
Graft vs.
Host Disease
Matching
Issues
Cost
* Source- http://marrow.org/HD/Payor/NP00159_Payor_Procurement_2011_09v4.aspxAccessed March 2012
www.cordlifeindia.com
www.cordlifeindia.com || 55
The stem cells from the cord blood you store will be a perfect
match for your baby. It is also a ready source of genetically
related stem cells for someone else in the family. Compared
to bone marrow or other sources of the stem cells, the
likelihood* of locating a matching cord blood unit is 60%
higher between siblings.
Who can use my

baby's cord blood?
100%
perfect match
for self
60%
Likelihood of locating
a matching cord
blood unit compared
to bone marrow
*Source: Based on 4 matches out of 6 human-leukocyte
antigen (HLA) markers
How is cord blood collected?

The collection of cord blood is a painless, safe and quick process which does not interfere with the birthing
process. After the baby is born, the umbilical cord will be clamped and within minutes, the obstetrician or
care giver will drain the cord blood into a sterile single use blood bag. The collection kit provided by Cordlife
contains all the necessary materials to complete the collection of cord blood.
Of course, the collection of cord blood is secondary to any medical issues that may arise during the birth and
the final decision to collect always resides with the obstetrician or care giver.
What does cord blood processing entail?

Other than stem cells, cord blood contains red blood cells, white blood cells and plasma. The objective of
cord blood processing is to extract as much stem cells as possible for final cryopreservation.
Cordlife uses Sepax2 processing technology, which harvests 99.46% stem cells.
How is Cord Blood

Collected, Processed
and Stored?
Sepax 2
Introducing India's first advanced automated processing technology Sepax 2. This Swiss made and FDA
Cleared device can recover as high as 99.46% Stem cells. This technology ensures 57% more cell count
higher than any other processing method.
Sepax 2 the future in stem cell processing technology:
Gives automated reading of the stem cells.
The yield from the cord blood processing is consistent, with high reproducible results
More cells are recovered / harvested from your baby's cord blood for future treatment (if needed), allowing
better chance of a successful transplant.
Complies with highest quality standards such as AABB and FACT-Netcord.
Over 75% public banks rely on Sepax 2 technology for processing.
Processes 2ml 220ml of Cord blood.
Takes only 25-35 minutes to complete the entire processing. Hence maintains maximum viability of the stem
cells.
Uses the cool mix technology to maintain the maximum viability of the stem cells.
How are cord blood stem cells stored?

When it comes to cord blood banking, we always insist on the highest standards so that our clients can have
improved transplant
outcome. Our cryogenic storage tanks are designed to feature precise temperature
0
control at -196 C. It is equipped with a monitoring system that scans for any out-of-range conditions to
ensure the temperature is maintained within optimum range. In case of emergency, our storage tanks can
run for 14 days without power or additional liquid nitrogen. This system provides the most secure, long-term
storage solution.
Features of Sepax2 and what it means to You, as a Parent

Features
Swiss Quality
Swiss-made patented automated cord blood processing system.
Advanced technology with new protocols
Fully Automated
Requires no external centrifuge or operator intervention during processing.
Controlled, smooth and precise separation by an advanced optical sensor.
Benifits to you
We process your babys cord blood
with high Swiss precision, reliable,
state-of the- art technology.
The yield from cord blood processing is
consistent, with reproducible high results
(that is cell recoveries).
Cell Recovery
Able to achieve as high as 99%1Total Nucleated Cells (TNC) recovery rate
Safety
Closed processing system.
Each processing step is fully monitored and traceable throughout the procedure.
Possesses a troubleshooting system. Even in cases of power failure, all cord
blood units can be reprocessed without having errors.
The only cord blood processing technology that processes one cord blood unit at
a time.
Conducts automated kit test before the start of procedure and pressure test to
check for any leakage.
It optimizes the sedimentation parameter for each cord blood unit.
Regulatory Approvals
Cleared by U.S. FDA, approved by European Union (evidenced by CE Mark),
and Japan MHLW (Ministry of Health and Labor Welfare).
Complies with the highest quality standards such as AABB & FACT-Netcord3.
Statistics/Evidence
An advanced cord blood processing technology with proven transplant track
record.
Worlds most tested and proven automated system.
Over 1 million cord blood units3 have been processed by the Sepax System in
over 50 countries, this is almost half of the worldwide processing procedures for
cord blood inventory.
Trusted by majority of the worlds leading public and private cord blood banks.
The Sepax system has been chosen by over 75% of the Netcord organizations
that group the leading public cord blood banks around the world.
Our investment in advanced technology
First Bank
now
and in the future means you and
Cordlife is the only private
cord
blood
bank
in
India
to
offer
automated
cord
blood
your family will benefit from any future
processing with Sepax 2.
technology upgrade for the long-term
storage of your babys stem cells.
References
1.Cordlife umbilical cord blood processing data as at 30 April 2013
2.Valeri et al., Transfusion 1996;36:303-8
3. Biosafe, manufacturer of Sepax (figures accurate as of 31 March 2013)
Top 10 reasons why you

should consider banking
your baby's cord blood
01
02
1 of the top 10 common cancers in India, Non-Hodgkin's lymphoma, is a

stem cell treatable disease.
03
2 of the top 5 most common childhood cancers in India, leukaemia and

lymphoma, are treatable with stem cells.
04
05
06
07
08
09
10
1 in every 200 people may need to use his or her stem cells in a lifetime.
1 in 500 babies has cerebral palsy, a neurological disorder incurred at

birth, which may be treated successfully with cellular therapy.
In the case of cellular therapy and regenerative medicine, only the

patient's own stem cells can be used in the treatment for best results.
A guaranteed match for autologous transplants (where the donor and

recipient are the same individual)
A readily available supply of stored haematopoietic stem cells. This compares
well to having to do a national or international search which is costly and timeconsuming in an already time critical situation.
No risk of Graft vs. Host Disease (GvHD) for autologous transplants, a
situation where the transplanted tissue attacks the patient's own tissue.
Ease of collection, which is pain-free and risk-free to both mother and

child.
Umbilical cord blood stem cells are younger, and are more tolerant to tissue
mismatches, compared to other types of stem cells, e.g. bone marrow.
Sources: http://www.cordlifeindia.com/cord-blood-banking/why-bank-cord-blood
What is umbilical cord?

Umbilical cord functions as a lifeline between mother and child during a pregnancy. After a baby is delivered,
the umbilical cord is cut and normally discarded with the placenta as medical waste. This procedure is
painless due to the lack of any nerves on the umbilical cord.
What are umbilical cord stem cells?

A baby's umbilical cord has a large number of Epithelial Stem Cells (EpSCs) and Mesenchymal Stem Cells
(MSCs). While EpSCs are primarily responsible for forming the outer protection layer of the body or organ
such as skin, liver, pancreas, brain and gastrointestinal tract, MSCs have proven its capability to differentiate
into neurons, cartilage and bone.
Current research with these cells revealed encouraging results in the treatment of a wide variety of medical
conditions including stroke and replacement of cornea membrane. In the future, once these advances
translate to successful therapies, any family who stored their child's stem cells will have this resource to
support these potential treatments.
What is Umbilical
Cord Banking?
MESENCHYMAL
STEM CELL
EPITHELIAL
STEM CELL
Gastro-intestinal Tract
Eye (Corneal)
Liver (Hepatogenic)
Skin (Keratogenic)
Pancreatic (Pancreatogenic)
Fat (Adipogenic)
Bone (Osteogenic)
Muscles/Cardiac Muscles
Cartilage (Chondrogenic)
Brain & Neurons
(Dopaminergic &Neuro)
Upon Explant Differentiation Culture, Bio

Marker testing and Tissue verification the
cord details will be stored with the Global
Cord Registry, an international databank
that enforces the Explant patents and
authenticates the processing origin and
quality of the stem cells, empowering the use of
patented cell extraction and animal protein free culture and
the capacity to use the cells for therapy.
1
How is Umbilical Cord 3
Collected, Processed
and Stored?
5
STEP1
After your baby's cord blood has

been collected, your obstetrician
or midwife will clamp and cut a
segment of the umbilical cord.
This cord will be stored in a sterile
container provided in your
Umbilical Cord Collection Kit in
dry condition.
STEP3
Next, the umbilical cord is further

processed, cleaned with wash
buffer solution and segmented
into small pieces.
STEP5
Once the cryoprotectant solution

has been added, the tissue sections
are frozen gradually in the
controlled-rate freezer, where the
temperature is lowered by 1-2C per
minute to preserve the viability,
potency and plasticity of the stem
cells.
STEP2
The umbilical cord collected is

then sent to Cordlife's laboratory
without being X-rayed during
transit for processing and
cryopreservation. Prior to
processing, the identity of the
umbilical cord collected is
verified by our laboratory
biotechnologist to ensure that the
unit belongs to the right client. All
subsequent steps are performed
in a biohazard safety cabinet to
further eliminate the risk of
contamination.
STEP4
The disinfected tissue sections

are then transferred into multiple
cryovials added with
cryoprotectant solution to
safeguard the viability of the stem
cells in the tissue sections during
cryopreservation.
STEP6
After the controlled-rate freezing

process, the cryovials are
transferred into a MVE anticontamination vapour-phase liquid
nitrogen storage system for long
term cryopreservation at -190C.
Diseases treated with stem cells:

The following is a list of some of the diseases that have been treated with cord
blood and other sources of similar type of stem cells (Haematopoietic Stem Cell),
like bone marrow and peripheral blood. Stem cell therapies continue to change
and evolve quickly.
Blood Cancers
Diseases Treated with

Stem Cells and its
Potential Applications.
Acute Myelogenous Leukaemia

Acute Lymphoblastic Leukaemia
Chronic Myelogenous Leukaemia
Histiocytic Neoplasms
Other Myeloproliferative Neoplasms
Myelodysplastic syndrome
Multiple Myeloma
Immuno deficiency Disorders

Chronic Granulomatous Disease
Common Variable Immunodeficiency
Severe Combined Immune Deficiency
(SCID)
Wiskott-Aldrich Syndrome
Metabolic Disorders
Adrenoleukodystrophy
Gaucher's Disease
Hurler Syndrome
Krabbe Disease
Metachromatic leukodystrophy
Osteopetrosis
Wolman Disease
Solid Tumors
Hodgkin Lymphoma
Non-Hodgkin Lymphoma
Langerhans' Cell Histiocytosis
Neuroblastoma
Retinoblastoma
Non-Malignant Blood Disorders

Aplastic Anaemia
Chediak-Higashi Syndrome
Congenital Dyserythropoietic Anaemia
Diamond-Blackfan Syndrome
DiGeorge Syndrome
Evans Syndrome
Fanconi's Anaemia
Glanzmann'sThrombasthenia
Leukocyte Adhesion Deficiency
Paroxysmal Nocturnal Hemoglobinuria
Pure Red Cell Aplasia
Sickle Cell Anaemia
Thalassemia Major
Hereditary BM failure
syndromes
Banking cord blood does not guarantee that the cells will provide a cure or be applicable in every situation. Use will be ultimately determined by the treating physician.
Clinical Trials:
With the advancement of stem cell* research, the potential for future use of stem cell grows. Below is a list
of diseases currently under Clinical Trials. These are diseases for which stem cell treatments have shown
to be beneficial, but have yet to be implemented as a standard treatment. Some of these stem cell
transplants only slow down the progression of the diseases, but do not produce a cure. For other diseases,
the stem cell treatments may result a cure, but the optimum dosage and usage of the stem cells are still
under study.
For the latest information, please visit www.clinicaltrials.gov
Experimental Treatments
These are randomised controlled trials conducted in a scientific laboratory involving in-vitro (test tube or cell
culture) and in-vivo (animal) work.
The experimental trials are in place to assist and to decide whether they have scientific merit for further
development / progression to clinical trials.
Cartilage repair
Cerebral palsy
Crohn's disease
Diabetes Type 1
Ewing Sarcoma
Multiple Sclerosis
Myocardial Infarction
Rheumatoid Arthritis
Spinal cord injury
Alzheimer's Disease
Amyotrophic lateral sclerosis
Congenital hydrocephalus
Huntington's Disease
Liver cirrhosis
Parkinson's Disease
Stroke
Systemic Lupus Erythematosus
Traumatic Brain Injury
Cord Blood Registry Website

[http://www.cordblood.com/cord_blood_banking_with_cbr/banking/diseases_treated.asp ], 3 August
2011
Parent's Guide to Cord Blood Foundation
[http://www.parentsguidecordblood.org/content/usa/medical/diseases.shtml?navid=37 ], 3 August 2011
National Marrow Donor Program
[http://www.marrow.org/PATIENT/Undrstnd_Disease_Treat/Lrn_about_Disease/index.html ], 3 August
2011
Clinical Trials [ http://www.clinicaltrials.gov/ ], 3 August 2011
The above list of diseases is a compilation from the above websites and other sources such asmedical literatures and journals. (*Stem cells mentioned
here comprises of other cell lines such as Epithelial and Mesenchymal stem cells & etc. The clinical trials and experimental treatments listed above may
be using other lines of stem cells, and not only Haematopoietic stem cells.)
An once-in-a-lifetime decision. Learn before you act.

Cordlife is more than just a cord blood bank. We are always at the forefront of science in search of sound
and emerging biotechnologies which may avail your family of more treatment options. The full potential of
cellular therapy goes beyond its current proven applications. With rapid development and ongoing
advancements in current therapy trials, any family who has stored their child's stem cells can be part of
this medical revolution.
It is with this in mind that we offer Total Stem Cell Banking Solution.
What is Total Stem Cell Banking?

With Cordlife's Total Stem Cell Banking, you have the opportunity to store 3 different cell types from 2
sources; your baby's cord blood and umbilical cord. These are Haematopoietic, Mesenchymal and
Epithelial Stem Cells. Storing all these 3 important cell types extends the potential of stem cell
treatments. Other stem cells from the cord are also preserved for possible future therapies.
Cordlife's Total
Stem Cell
Banking Solution.
Service Type
Cord Blood Banking
Advanced Umbilical Cord Banking
Sources of stem cells
Cord Blood
Umbilical Cord
Types of stem cells
Haematopoietic stem cells (HSCs)
Types of stem cells for
Proven
Potential
Cancers such as
Leukaemia and
Lymphoma,
Blood disorders
such as
Thalassaemia,
Metabolic
diseases, immuno
deficiencies
Heart disease Stroke,

Type 1
Diabetes
Neurological
regeneration of the
brain and spinal cord,
liver diseases
* Refer to Page 4 for full

list of HSCs treatable
diseases.
Cordlife's Exclusive:
Epithelial stem cells (EpSCs)
Potential
Wound / Soft Tissue Repair:
Surface ulcers (diabetic ulcers
etc) Various type of burns other
tissue trauma
Organ Regeneration: Liver,
Pancreas, Gastrointestinal trace
healing
Eye: Replacement of cornea
Mesenchymal stem cells

(MSCs)
Potential
Tissue Repair: Myocardial infarction,
smooth muscle repair, Osteoarthiritis
(Cartilage repair and other orthopedic
applications), Stroke, Pulmonary fibrosis
Haematopoietic engraftment Support:
Shorten time of engraftment; increase
success rate of transplant
Immune Modulation: Type 1 diabetes,
Crohn's disease, eases the severity of
Graft Vs. Host Disease in renal and
hepatic transplants.
Sepax2
Sepax2 - India's first automated
processing technology that recovers
99.46% stem cells.
Quality Guarantee
Cordlife's
360 Degree Protection
for Your entire family .
Cordlife Quality Guarantee- worth US$

30,000.
Natural Calamity
Relief Program
Extensive protection in case of unforeseen
circumstances if your stem cells are
destroyed Cordlife shall provide US$
25,000.
Cordlife Cares
Program
Cashless medical assistance for your
family-Cordlife provides a financial
assistance of US$25,000 for your family for
a stem cell transplant.
Worldwide
Free Transport
Worldwide free transportfree
transportation of your stem cells around the
world.
You will receive reports to prove the viability of two

cell lines in both tissue and expanded cell form.
The technology is capable of isolating two types of

cell lines mesenchymal and epithelial stem cells
from the umbilical cord.
Explant, Differentiation
& Culture
Isolated cells are capable of further expansion under

artificially controlled conditions outside the human
body as per the requirement from day one
maintaining the uniqueness.
Developing the liver cells capable of producing Albumin,

Alpha fetoprotein and the mucus membrane like cells
producing Mucin from the differentiated cell lines gives the
ultimate proof to the functional capacity of the cells.
Tissue verification is the ultimate proof of

(i)Viability (ii) Potency (iii) Plasticity ( i v ) T r a n s
differentiability.
CordSURE BIOCORD
CordSURE BIOCORD is a basic Cordlife service package where stem cells from your baby's cord blood and
the umbilical cord tissue are collected processed and stored in India.
CordSURE EXPLANT
CordSURE EXPLANT is an advanced service package which consists of both long-term storage of stem
cells from your baby's cord blood and umbilical cord tissue. Your baby's umbilical cord tissue will be
processed and validated by Global Cord Registry in Singapore using their Patented Explant Differentiation
Conditioning and Culture Media. This important step will ensure the maximum viability of up to 4 billion cells
per cell line.
CordSURE EPICORD 50M & 500M

CordSURE EPICORD 50Mor 500M is the ultimate service package that ensures the long-termstorage of
your baby's cord blood stem cells as well as 50 million or 500 million, each of mesenchymal stem cells and
epithelial stem cells from the umbilical cord. Once the stem cells from the umbilical cord have been cultured
andexpanded using the Patented Explant Expansion Media by Global Cord Registry in Singapore, theywill
be stored both in Singapore and India.
Our Services
Cordlife Limited is a leading healthcare group of companies that provides a full suite of umbilical cord and
cord blood banking services to expectant parents for the collection, processing and cryopreservation of
cord blood and umbilical cord stem cells . The Group is dedicated to offering the highest possibility of
successful adult stem cell therapy to give hope and save lives. Listed on the Australian Securities
Exchange, Cordlife Limited is well established as the largest network of private stem cell banks across
Asia Pacific with state-of-the-art facilities in India, Indonesia and the Philippines as well as an affiliate
network that extends to Singapore, Hong Kong, and China.
Expertise and quality you can trust

The Group's in-depth knowledge of stem cell banking practices and standards is well recognized by
numerous world-class quality standard organizations as well as country regulators in the Asia Pacific
region. In 2007, Cordlife Limited was bestowed the prestigious Technology Pioneer title by the World
Economic Forum which further validated the Group's outstanding performance in the industry.
About Cordlife India
Cordlife Limited
at a glance
Cordlife India is a majority-owned subsidiary of Cordlife Limited, a company listed on the Australian
Securities Exchange. A winner of Technology Pioneer award conferred by the World Economic Forum,
Cordlife has accumulated more than 10 years of experience in stem cell processing and
cryopreservation.
Cordlife Limited owns and operates 3 state-of-the-art stem cell processing and cryopreservation facilities
in India, Indonesia and the Philippines along with an affiliate network extending to other full-fledged
facilities in Singapore, Hong Kong and Mainland China.
About Cordlife's Facility in India

Cordlife India is the largest and most advanced cord blood facility in the country, with storage capacity of
up to 150,000 cord blood units. The state-of-the-art laboratory with full processing, testing and
cryopreservation capabilities is equipped with continuous power back-up as well as the most-up to-date
security and surveillance systems. In addition, the building is specially designed to withstand any natural
calamity. Cordlife India is licensed by the Drug Controller General of India to collect and process cord
blood as well as cord tissue from and all Indian states and cities.
Technology Pioneer 2007 (World Economic Forum)

ISO 9001:2000 Certificate (Hong Kong)
DEPKES (Indonesia)
Globally Accredited
and Recognized
Ministry of Health Certification (Singapore)
AABB Accreditation
Ministry of Health Certification (India)

TGA Accreditation (Australia)
ISO 9001:2008 Certification (India)

World Economic Forum 'Technology Pioneer' Status

Over the years, our Group has achieved several firsts - one of which being the world's first and only cord
blood bank to be awarded the prestigious 'Technology Pioneer' title, conferred by the World Economic
Forum in recognition for advancing in the field of adult stem cell therapy, stem cell banking and
technologies. Cordlife is the world's first and only private stem cell company to receive this honor.
Quality and Protection Guarantee

Stem cell banking is parents' first and most precious gift for their children. Keeping this in view Cordlife offers
the only Quality Guarantee in India, which ensures cord blood replacement or a compensation of
US$30,000, should the stem cells lose their viability.
Long Term Financial Stability

Stem cell banking can provide a lifetime of protection for many families. That's why it is imperative for them
to choose a highly experienced, technically advanced and financially stable stem cell bank. Cordlife India is
a majority owned subsidiary of Cordlife Limited listed on the Australian Securities Exchange. With Cordlife
Group's robust growth history, strong financial backing and well-positioned plan to ensure long term safe
and secure storage of stem cells, you can confidently entrust your baby's stem cells to our care.
Quality You Can Trust
Confirmation of Assurance
Cordlife is the first and only company in India to introduce the novel and patented technology of isolating,
differentiating and expanding the two cell lines (EpSCs and MSCs) from the umbilical cord lining confirming
the viability and functionality of the stem cells.
Advantages of our processing method

Cordlife uses its unique patented technology to process and cryopreserve the cord tissue derived
Mesenchymal and Epithelial stem cells. Cordlife's technology ensures maximum harvesting of stem cells at
the time of need.
Maintain the original characteristics of stem cells:

The cord tissue is cut into multiple pieces, placed in multiple petri-dishes and then processed; thereafter the
unexpanded, undifferentiated stem cells are put in multiple vials and stored in mesenchymal growth storage
medium and epithelial growth storage medium.
Retain the potential of all stem cells:
Advantages of
Cordlife's Processing
and storage methods
Storing tissue sections of the umbilical cord is the safest and most reliable way to store all the stem cells
which can be derived from the amniotic membrane, Wharton's jelly, perivascular and peri-allantoic tissue,
which contain other potentially valuable stem cells yet to be identified, opening up more medical options for
your family in the future.
Expansion of stem cells:

Cordlife has the technology to expand the cord tissue derived stem cells multiple folds. Cordlife provides you
the option to expand the stem cells into 1 billion (500 million each) as cases like ulcers, burns and accident
require prompt treatment and theseexpanded stem cells can be provided for immediate use. However, the
stock of unexpanded and undifferentiated stem cells is kept intact. Moreover the use of animal protein and
membrane and carcinogenic cell extract free media ensures a safer and more acceptable yield.
33
Advantages of Cordlife's storage method : Multiple

cryovials
Cordlife uses multiple cryovials to store umbilical cord tissue
sections. By storing in cryovials, you have the option to
withdraw one cryovial at a time without affecting the viability
ofthe tissue sections in other cryovials. The cryovials are
further wrapped inside an overwrapbag to further safeguard
against cross contamination. When it comes to umbilical
cord blood banking, we always insist on the best so that our
clients can have better transplant and cellular therapy
outcome. The cryogenic storage tanks used by Cordlife,
MVE Anti-contamination vapour-phase Liquid Nitrogen
Storage System, have been used and trusted by the majority
of private cord blood banks in the world for decades. Unlike
other storage systems available in the market today, our
tanks have never been recalled by the U.S. FDA or the
manufacturer.
MVE Anti-contamination Vapour-phase Liquid

Nitrogen Storage System Designed for optimum
performance
Umbilical cord stem cells remain at cryopreservation
condition as long as they are stored below Glass Transition
Temperature (-135C). MVE system is designed to feature
precise temperature control at -190C, well below the
minimum storage temperature.
MVE system is equipped with a monitoring system that
scans for any out-of-range conditions to ensure the
temperature is always maintained at an optimum range
A liquid nitrogen reservoir is present below the sample
platform. This will typically maintain the desired temperature
for the samples in the storage tank for more than 20 days due
to vapour created by the liquid nitrogen.
Moinam was just 4 months old when he was found to be suffering from E-Beta thalassaemia, his parents went all over India
in search for a cure. But wherever they went they found onlydisappointment until they learned that stem cell transplant is the
only permanent cure. During this time young Moinam went through a hard time because of regular blood transfusions, high
dose medications and a deteriorating health. The parents opted for stem cell therapy and planned for a second baby so that
the cord blood stem cells of the newborn could be used to save young Moinam's life. During his baby sister Ahoma's birth
her stem cells were collected and stored with Cordlife so that it could be used for transplant. The cord blood stem cells along
with bone marrow stem cells of Ahoma were used for this unique treatment, first such case in India. Since transplant young
Moinam has been showing signs of recovery and is already a figure of inspiration for many such suffering from this disease.
Young Moinam has started showing promises of improvement; his hemoglobin level has considerably increased which proves the engraftment has taken
place. He needs to be careful for another 3 months to ensure that his body gets used to the environment and then he can go back to a normal life.
Successful Release of
Stem cells for
Transplant and
Cellular Therapy
Dr. Ashish Mukherjee, Transplant Physician & Director Netaji

Subhash Chandra Bose Cancer Research Institute
Sou
rce H
stan
Time
s
, 7th
2011
09
grap
e Tele
: Th
ource
May
er 20
cemb
De
h, 3rd
An accident at birth 2 years ago deprived Baby Georgia of

oxygen to her brain, resulted in cerebral palsy. The damage
to the toddler's brain caused her to move involuntarily,
suffer from muscle spasms and have up to 50 seizures a
day. It was only after Cordlife put the family in touch with a
renowned neurosurgeon that enabled Georgia to be
infused with her own cord blood, also stored by Cordlife.
Her conditions have improved since then.
Georgia has made good progress since her stem cell infusion. She has better
visual focus and eye contact with adults during play. She has more vocalization
and tries to 'sing-a-long' by making different sounds to nursery rhymes. Georgia
has improved postural and head control and is observed to be reaching more with
her arms.Ms. Jaclyn Tan, Consultant Occupational Therapist at OzWorks
Therapy
indu
004
, 1 July 2
its Times
he Stra
ource: T
Little Ryan was suffering from leukaemia at a tender age of

three. Despite a nation-wide search for bone marrow stem cells,
a suitable donor could not be found. Fortunately, baby sister of
Ryan, Rachel was born during that critical period which enabled
the family to store Rachel's cord blood with Cordlife, for Ryan's
transplant. Now 13, Ryan is in school and his leukaemia is in
remission.
We were elated when Rachel's blood was shown to be a 100-per-cent match for Ryan.
The healthy cells from his sister re-populated in his bone marrow and revived his
immune system.Dr. Tan Ah Moy, Head and Senior Consultant for Haematology and
Oncology services at the KK Women's and Children's Hospital where the transplant was
performed.
We learnt about stem cells from our

close friend. Our baby motivated us
to go for it.It is just for his good health
& to cope up with maximum future
health problems. -
Shiv Sagar Singh (cricketer)
"I chose to do cord blood banking because I wanted my child

to have every advantage in life that I am able to give him. I
was initially attracted to Cordlife because of the
professionalism and responsiveness of its personnel. From
the outset, the Cordlife representative when I dealt with
promptly, patiently and ably addressed my various concerns
such as those pertaining to technology involved, and the
long term viability of the company. To be able to feel secure
for my baby is like an unchained melody of life now." -
Client Testimonials
Anindya Chatterjee
(lead vocalist Chandrabindoo band ,Kolkata )
I am a kind of person who does a lot of research before taking a

decision in anything important like this. Out of the stem cell banks
that I consulted before registration, I personally liked and highly
recommend Cordlife for multiple reasons. First, I have to really
appreciate their level of service which has over exceeded my
expectations. The stem cell collection was done professionally.
The collection lady stayed with us for 48 hours in the hospital
patiently for the normal delivery to happen. After the collection I received my
certificates stating that the stem cells were stored successfully in no time. Out of my
personal experience I had with Cordlife, I strongly recommend their services. Finally I
am relieved that my babys stem cells are in good hands. Thank you Cordlife for
making it happen. -
Families that believe Health is Wealth
Krishna Molugu (Hyderabad)
I was intrigued by the concept of cord blood banking during my

pregnancy. Fortunately I was able to source Cordlife and was
immediately given information and assistance. Understanding the
importance and long term benefits of preserving cord blood for any
eventuality involving the health and well being of my baby; it is a
priceless gift and support that I have chosen to give my baby.
Shilpa Shetty (Actress, Mumbai)
Contact us 1800-345-6200 or +91 9830166200

to enquire more about banking your baby's
umbilical cord and cord blood stem cells. A
collection kit will be given to you upon your
enrollment with us.
Carry your collection kit to the birthing centre

and inform us upon your admission. A small
amount of blood will be drawn from the mother
which will be tested for infectious diseases.
Five simple steps

to Bank with Cordlife
After your baby is delivered, the umbilical cord will

be clamped and cut. Your caregiver will drain the
cord blood into a sterile blood bag provided and
umbilical cord will be placed into a sterile container
provided by Cordlife.
After the collection of your baby's

umbilical cord and cord blood, the unit
will be sent to our facility within 36 to 48
hours.
Your baby's umbilical cord and cord

blood will be processed and harvested
by our qualified technicians under sterile
environment and stored permanently at 190C.
New Born Screening for your baby:

Newborn screening is the practice of testing every newborn for certain harmful or potentially fatal disorders
that aren't otherwise apparent at birth. The baby may appear to be healthy but may be born with medical
conditions which may affect the babys mental and physical growth. This screening detects such disorders
and helps prevent serious outcomes.
New Born Screening a practice accepted across 52 countries to ensure a safe healthy
future for your child.
Japan. - > 99 %
U.K. - > 99 %
China -60 %
USA - 100%
INDIA
<1 %
S.A. ~ 40 %
S.E. Asia
95-99%
Australia
100%
Source:
Padila, Ann Acad Med Singapore 2008;37(Suppl 3):6-9
NHS , U.K, 2011 data http://www.labnews.co.uk/features/childs-play-newborn-screening-in-the-uk/
Borrajo, 2007, J Inherit Metab Dis, 30:466-481
Padilla et al, J Community Genet (2012) 3:3545
Some of the genetic disorders that can be detected at early stage and even prevented with
simple dietary measures
Asthma
Cancers
Cleft Palate
Diabetes
Heart Disease
Hyper tension
Obesity
Epilepsy
Speech Difficulties
Pigmental defect
Lethargy
Learning disabilities
Hypotonic
Hyperactivity / Aggression
Why new born screening?

1)Tests and protects your Baby against 110 disorders.
2)No pricking no puncturing required we use state of the art technology to test the samples through urine samples.
3)Safe and painless procedure.
4)It can be done at your convenience even after you are discharged from the hospital.
5)Easy as it comes just collect the sample and courier us the sample at our address and we will take care of the
rest .

Stem Cell Revolution

Transféré par

Informations du document

Titre original

Copyright

Formats disponibles

Partager ce document

Partager ou intégrer le document

Options de partage

Avez-vous trouvé ce document utile ?

Ce contenu est-il inapproprié ?

Droits d'auteur :

Formats disponibles

Stem Cell Revolution

Transféré par

Droits d'auteur :

Formats disponibles

Introducing Indias first

Automated Processing Technology

TAKING THE FIRST

Dear Expectant Parent,

What is cord blood?

What are cord blood stem cells?

Other types of cells :

Blood and Immune cells :

What is cord blood &

Red blood cells

White blood cells

Advantages of cord blood stem cells

Donor needs to be injected multiple

At the time of delivery.

Requires extensive donor Search.

Younger and more

Higher chance of complications after infusion.

Requires a perfect match between donor and recipient

Can cost as high as USD30,000*

100% ownership with

* Source- http://marrow.org/HD/Payor/NP00159_Payor_Procurement_2011_09v4.aspxAccessed March 2012

Who can use my

How is cord blood collected?

What does cord blood processing entail?

How is Cord Blood

How are cord blood stem cells stored?

Features of Sepax2 and what it means to You, as a Parent

Top 10 reasons why you

1 of the top 10 common cancers in India, Non-Hodgkin's lymphoma, is a

2 of the top 5 most common childhood cancers in India, leukaemia and

1 in 500 babies has cerebral palsy, a neurological disorder incurred at

In the case of cellular therapy and regenerative medicine, only the

A guaranteed match for autologous transplants (where the donor and

Ease of collection, which is pain-free and risk-free to both mother and

What is umbilical cord?

What are umbilical cord stem cells?

Upon Explant Differentiation Culture, Bio

After your baby's cord blood has

Next, the umbilical cord is further

Once the cryoprotectant solution

The umbilical cord collected is

The disinfected tissue sections

After the controlled-rate freezing

Diseases treated with stem cells:

Diseases Treated with

Acute Myelogenous Leukaemia

Immuno deficiency Disorders

Non-Malignant Blood Disorders

Cord Blood Registry Website

An once-in-a-lifetime decision. Learn before you act.

What is Total Stem Cell Banking?

Cord Blood Banking

Advanced Umbilical Cord Banking

Sources of stem cells

Types of stem cells

Haematopoietic stem cells (HSCs)

Types of stem cells for

Heart disease Stroke,

* Refer to Page 4 for full

Mesenchymal stem cells

Cordlife Quality Guarantee- worth US$

You will receive reports to prove the viability of two

The technology is capable of isolating two types of