Académique Documents
Professionnel Documents
Culture Documents
at
Content
1) About TRI.
2) About Biochemistry Division.
4) Analysis of tea samples.
5) Development of a method for manufacturing
paper from refuse tea.
About TRI...........................
Mechanization
Productivity improvement
Technology Transfer
Societal - Economics
Reference: http://www.tri.lk/research-and-development/biochemistry
-Pluck
-Pluck tea
tea as
as bud
bud with
with two
two leaves
leaves
Rolling
Rolling and
and CTC
CTC
-Brake
-Brake the
the tea
tea leaves
leaves into
into small
small particles
particles
Fermentation
Fermentation
-- Keep
Keep the
the tea
tea for
for oxidation
oxidation of
of polyphenols
polyphenols
Firing
Firing (Drying)
(Drying)
-Reduce
-Reduce the
the moisture
moisture of
of fermented
fermented tea
tea to
to 2%-3%
2%-3%
Grading
Grading
-Grade
-Grade the
the tea
tea based
based on
on particle
particle size
size and
and amount
amount of
of
fiber
fiber
Dust 1
BOPF
BOP
Peko
Dust 2
BM
Refuse tea
Refference:http://ceylonteainfo.com/recent-developments
--Extract
Extracttea
teainto
intohot
hotwater
waterfrom
fromrefuse
refusetea
tea
Filtering
Filtering and
and centrifuging
centrifuging
--Removal
Removalof
ofparticle
particleof
ofspent
spenttea
tea
Concentrating
Concentrating
-Concentrate
-Concentratethe
theextract
extractuntil
untilsolid
solidparticle
particlelevel
levelreaches
reaches24%
24%
Spray
Spray drying
drying
-Powder
-Powderof
ofinstant
instanttea
teais
isprepared
prepared
b)
c)
OH
OH
OH
OH
HO
OH
H
O
HO
HO
OH
OH
OH
OH
(-)-catechin
OH
theaf lavin
OH
OH
OH
H
OH
HO
O
H
OH
(-)-epigallocatechin
Theory
OH
HO
HO
OH
3(W 8O 23)(aq)
phosphotungstic acid
(aq)
(aq)
3H 3PO 4(aq)
COOH
COOH
gallic acid
OH
HO
HO
OH
phosphomolibtictic acid
3H3PO4(aq)
COOH
gallic acid
COOH
sample
- Dintercept)Vsample
S m
std
sample
d100
10000 w
DM, sample
where,
Dsample - the optical density obtained for the sample
test solution;
Dintercept- the optical density at the point the best-fit
linear calibration line intercepts the y-axis;
Sstd
Procedure :
Preparation of samples
The
The tea
tea leaves
leaves were
were ground
ground
Moisture
Moisture of
of the
the tea
tea sample
sample was
was determined
determined
0.200g
0.200g of
of the
the sample
sample was
was measured
measured
10.00
10.00 ml
ml extraction
extraction of
of 70%
70% methanol
methanol was
was prepared
prepared
(Solution
(Solution A)
A)
1.00
1.00 ml
ml of
of AA was
was diluted
diluted up
up to
to 100.00
100.00 ml
ml with
with distilled
distilled
water
water(Solution
(SolutionB)
B)
Standard preparation.
Series of gallic acid solutions were prepared
according to the below table.
Table 1: Concentration of gallic acid solution
Gallic acid standard
solution
Concentration of
solution/(g/ml)
10.01
20.02
30.03
40.04
50.04
Taking measurements:
1.00
1.00 ml
ml from
from solution
solution was
was poured
poured into
into aa graduated
graduated test
test
tube
tube
5.00
5.00 ml
ml of
of 10%
10% Folin-Ciocalteu
Folin-Ciocalteu phenol
phenol reagent
reagent was
was
added
added
After
was
After 44 minutes
minutes 4.00
4.00 ml
ml of
of 7.5%
7.5% Na
Na22CO
CO3(aq)
was
3(aq)
added
added
The
The solution
solution was
was mixed
mixed and
and kept
kept for
for 60
60 minutes
minutes
Absorbance
Absorbance was
was measured
measured at
at
765
765 nm
nm
Results :
Table 2: Average absorbance of standard solutions
Gallic acid
standard
solution
Absorbance
Concentration
(0.0001)/
of solution/
Arbitrary
(g/ml)
units
10.01
20.02
30.03
40.04
50.04
0.1667
0.2709
0.3943
0.5283
0.6461
0.7
Absorbance/
arbitori unit
0.6
0.5
0.4
0.3
0.2
0.1
0
0
10
20
30
40
50
60
Sample no
Absorbance
Mass of tea
total
(0.0001)/
(0.0001)/
polyphenol
(arbitori content (%)
g
units)
0.2004
0.5051
20.32
0.2008
0.5540
22.39
0.2003
0.4785
19.17
Conclusion :
Average % of total polyphenol content based on dry
mater of sample is 20.63%
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Development of a method
for
manufacturing paper
from
refuse tea
Procedure :
Pulp preparation.
Refuse
Refuse tea
tea was
was mixed
mixed with
with boiling
boiling water
water for
for 10
10
minutes
minutes
Extract
Extract was
was filtered
filtered and
and washed
washed with
with 200
200 ml
ml of
of
boiling
boiling water
water
the
the residue
residue was
was added
added to
to aa beaker
beaker containing
containing NaOH
NaOH
and
and boiling
boiling water
water
The
The mixture
mixture was
was mixed
mixed
The
The solution
solution was
was filtered
filtered
The
The residue
residue was
was ground
ground step
step wise
wise with
with
water
water
The
The mixture
mixture was
was filtered
filtered and
and washed
washed with
with water
water
Pulp
Pulp
http://homepage.cs.uiowa.edu/~jones/prairiepaper.htm
Used
refuse tea
Amount
Used
NaOH
amount
/g
/g
5.00
8.00
25.00
3.500
2.000
1.250
(0.0001) (0.001)
A
B
C
Mixing
time/
minutes
Grinding
Time/
minutes
5
30
30
1
3
3
Producing paper:
In procedure A:
The
The pulp
pulp was
was put
put on
on to
to aa net.
net.
Another
Another net
net was
was laird
laird on
on it.
it.
It
It was
was pressed
pressed and
and water
water was
was removed
removed
Paste
Paste was
was kept
kept to
to dry.
dry.
Paper
Paper
In A and B procedure:
The
The pulp
pulp was
was put
put on
on to
to aa sieve
sieve dipped
dipped in
in water
water
Particles
Particles were
were allowed
allowed to
to suspended
suspended
It
It was
was kept
kept it
it to
to drain
drain water
water for
for over
over night
night
It
It was
was pressed
pressed
It
It was
was kept
kept to
to dry
dry
Paper
Paper
Results :
Table 1: NaOH used per 100g of refuse tea for
each procedure
Procedure
5.000
3.500
70.000
8.000
2.000
25.000
25.000
1.250
5.000
High
High
Strength
High
High
Low
Colour
Golden
yellow
Paper of
Procedure
A
Paper of
Paper of
Procedure Procedure C
B
Smoothness
of surface
Strength
Colour
1
1
1
2
2
3
Conclusion:
NaOH amount added for pulp preparation and the
time of mixing and grinding the pulp should be
high to get a best quality paper.
Grind refuse tea is not suitable for getting paper
with higher strength
Suggestion:
In this process the tea extraction ,extracted
previously can be used to manufacture instant tea.
The NaOH extraction can be used to extract
protein. So that the paper is manufacture from
waste product of above processes.
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