Discussion :

From this experiment, we was conducted the experiment to observe the part
of the plant stem, root and leave between monocot and dicot under light
microscope to understand and differentiate the location and morphology, and
function of the plant cell.
Firstly, the thinnest cut was obtain through the cross section of the plant A
stem, then mounting on the slide. We was observed cross section of the plant A
stem. The plant A is the garden blossom scientific name Impatiens balsamina. The
observation of this plant stem was labelled shown in figure 1. From the observation,
the plant A was a dicot based on their structure consists of cortex and pith. The
plant cortex is the tissue located between the vascular bundles and epidermis. The
cortex is responsible for the storage of photosynthetic products and the uptake of
water and minerals. It also consists of a large amount of thin-walled parenchyma
cells, which maintain the structure of a plant. Pith is a tissue in the stems of
vascular plants. Pith is composed of soft, spongy parenchyma cells, which store and
transport nutrients throughout the plant. From this structure, we can differentiate
the plant either the plant dicot or monocot. The monocot plant absence of this
structure. After that, we observed the prepared slide provided sunflower Young
Stem Helianthus, and monocot stem. From this observation we clearly can
differentiate the structure between the dicot and monocot stem. We had also search
on the internet from the google engine on the comparison structure of the stem
between monocot and dicot.
For the next experiment, we had observed the plant A leaves under the light
microscope. Leave is the place where the plant makes their own food through
photosynthesis. Leaves generally consist of a flattened blade and a stalk, petiole,
which join the leaf to a node of the stem. There are the difference between monocot
and dicot. They are differ in the arrangement of veins, the vascular tissue of leaves.
Most monocot has parallel veins while dicot leaves have a branching veins. The
thinnest layers of the slide were cut and mount on the slide and then was observed.
From the observation, we can identify the plant A leaves are dicot plant by
comparing with the prepared slide. From our observation, the plant A sample was
similar with the sunflower leaves prepared slide. The leaf consist of several
structure include xylem and phloem, stomata, and cuticle. Xylem function it
conveys water and dissolved mineral upward from roots into the shoot. Phloem
functions in transport organic nutrients from where they are made to where they are
needed. Stomata are located at the bottom of the leaves. It control the water loss of
the plant by the opening and closing of the guard cell. Then, cuticle located at the
outer layer of the leaf by covering the leave. It was waxy substance functioning to
prevent water loss.
For the last experiment, we had observed the several prepared slide of the
root. The prepared slide included monocot and dicot root, sunflower root, and Zea

mays. Firstly we observed the monocot and dicot rot. From the observation, there
are several different between them. The dicot root, the cortex is comparatively
narrow while for monocot root the cortex is wide. Then, for the dicot root the
pericycle is single layered while for the monocot root the pericycle is multilayered.
Then, pericycle produces lateral roots cambium and cork cambium for the dicot root
while pericycle produces lateral root for the monocot root. Xylem vessels are
angular for the dicot root while xylem vessels are oval and rounded for the monocot
root. Then, the dicot root the pith is absent while for the monocot root the pith is
well developed. For the Zea Mays root prepared slide, it was the monocot root. The
structure was similar to the monocot root by comparing both slides. The component
of the root was labelled in the figure 4.1. It was observed at magnification 100x and
200x under the light microscope. For the sunflower root prepared slide, we
compared with the slide with the other root slide and we can conclude the sunflower
root was the dicot roots

References :
1. Salisbury, F. B., & Ross, C. W. (1969). Plant physiology. Belmont, CA:
Wadsworth Pub.
2. Romberger, J. A., Hejnowicz, Z., & Hill, J. F. (1993). Plant structure: Function
and development: A treatise on anatomy and vegetative development, with
special reference to woody plants. Berlin: Springer-Verlag.
3. Plant structure and their function , retrieved from :
https://www2.estrellamountain.edu/faculty/farabee/biobk/BioBookPLANTANAT.
html