Bode Technology 10430 Furnace Road, Suite 107 Lorton, VA 22078 Phone: 703-646-9740 ‘Supplomental Forensic Case Report ‘May 12, 2008 To: ‘Andy Horita BODE Case #: 2507-101 Boulder District Attorney's Office ‘Agency Case #: 96DA21871 17778" Street Boulder, CO 80302 List of Evidence Received on April 18, 2008 for possible DNA analysi Sample# Agency Description 2807-01-07 Labeied es "Barbie Nightgown BPD#12KKY, CBIEI7" :2807-101-07A ‘exterior and interior of the bottom front of nightgown 2807-101-078 exterior of the left shoulder region front and back of nightgown 2807-101-076 ‘exterior ofthe right shaulder region front and back of nightgown 2807-101-07D ‘exterior and interior of the botiom back of nightgown Table One contains Profiler Plus and Cofiler data submitted on March 12, 2008 for the following samples: Bpp# cer Description co1Be 148 Blood standard JonBenet-Victim SRTG 368-2 Blood Standards Burke Ramsey-Brother 4RTGIOBOTET 356-2 Blood Standards Patricia Ramsey-Mother SRTGIOBSTET 348-2 Blood Standards John B. Ramsey-Father 2RTGITZTET 338-2 Blood Standard Melinda Ramsey- % Sister (Common Father) ARTS 328.2 Blood Standards John Andrew Ramsey- % Brother (Common Father) DNA PROCESSING, RESULTS, and CONCLUSIONS: lem 2807-101-07 was processed for DNA typing by anclysis of the 13 CODIS Short Tandem Repeat loci, the D281338 locus, the D198433 locus, and the gender determining locus Amelogenin using the AmpFLSTR® Identifler® kit Appropriate positive and negative controls were used concurrently throughout the analysis. The DNA profiles reported in this case were determined by procedures that have been validated according to standards established by the Scientific Working Group on DNA Analysis Methods (SWGDAM) and adopted as Federal Standards, 41. The DNA profile obtained from sample 207-101-07A contains a mixture of at least two individuals including the victim and at least one male coritibutor. The individuals associated with ‘samples John B. Ramsey, John Andrew Ramsey, and Melinda Ramsey are excluded 2s possible contributors to the mixture profile obtained from sample 2S07-101-07A. The individuals ‘associated with Burke Ramsey and Patricia Ramsey cannot be excluded as possible Contributors {o the mixture DNA profile obtained from sample 2S07-101-07A. ‘Tre probabilty of randomly selecting an unrelated individual who would be included as 2 possible Contributor to this mixture atthe core CODIS loci (excluding CSF1PO, D138317, DSS818, and. FGA) is: 1 in 50.0 Thousand in the US Caucasian Population 1 in 220.0 Thousand in the US African American Population 1 in 43.0 Thousand in the US Southwest Hispanic Population 1 in 58.0 Thousand in the US Southeast Hispanic Population Page 1 of 4BODE Case #: 2807-101 Date: May 12, 2008 ‘Agency Case #: 960421871 2. The DNA profile obtained from sample 207-101-078 contains a mixture of atleast two individuals inc'uding the victim. The individuals associated with samples John B. Ramsey, John ‘Andrew Ramsey, and Melinda Ramsey are excluded as possible contributors to the mixture profile obtained from sample 2S07-101-078. The individuals associated with Burke Ramsey and Patricia Ramsey cannot be included or excluded as possible contributors to the mixtufe DNA. profile obtained from sample 207-101-076, 3. The DNA profile obtained from sample 2S07-101-07C contains @ mixture of atleast two individuals including the victim and at least one male contributor. The individuals associated with ‘samples John B. Ramsey, John Andrew Ramsey, and Melinda Ramsey are excluded as possible Contributors to the mixture profile obtained from sample 2S07-101-07C. The individuals associeted with Burke Ramsey and Patricia Ramsey cannot be included or excluded as possible ‘contributors to the mixture DNA profile obtained from sample 2807-101-07C. ‘4, The DNA profile obtained from sample 2507-101-07D contains a mbcture of at least two individuals including the vietim and at least one male contributor. The individuals associated with ‘samples Patricia Ramsey, John B. Ramsey, John Andrew Ramsey, and Melinda Ramsey are ‘excluded as possibie contributors to the mixture profile obtained from sample 2S07-101-070. The Individual associated with Burke Ramsey cannot be included or excluded as a possible Contributor to the misture DNA profle obtained from sample 207-101-070. ‘See Table Two for summary of alleles reported for these samples. NoTEs: 1. supplemental report was issued due to an additional submission of evidence. Please see original report dated March 24, 2008. 2. The DNA extracts and submitted evidence will be retumed to Andy Horta at the Boulder District Attorney's Office, Report submitted by: hry Dd ‘Amy Jeanguenat, MFS DNA Analyst I Page 2 of 4 DarraszBODE Case #: 2507-101 Date: May 12, 2008, ‘Agency Case #: 960421871 Table One: Analysis of Short Tandem Repeat Loci | ‘eaeet | ouno | femem | some | mee Papa | Locus Ramsey | Ramsey | Ramsey | Ramsey | Ramsey | ‘Ramsey ‘AMEL x xy xx xy, xx xY | 381358 16,17 17,18 14,47 16,18 15,18 15,18 | | FoA zo2s | 22a | 202% 2324 2128 7024 | Desti7e | 12,13 12.43 rata | 13.18 13,13 13.14 | paist1 | 29922 | 29922 | 20922 | 20022 | 22922 | 29012 press1 | 14.6 13,16 tata | 1416 16.18 12.14 pssst | 11,12 waz | te 12,13 13,13, 44,12 i pissy | 11.12 te | tn 12,42 an | | rsa | 810 38 0,12 98 910 | i Diess39 | 9,12 12,12 1412, 9,12 12,13, 912 } Tor | 9308 87 793 Z 793 | esripo | 1013 | 10.18 12.13 70,11 70,12 i in inconclusive, no resus ‘p= net performed aint results Page 3 of 4 fester710 abed ‘Ays08fzow0y 10} ploysenty mojaq efeiy— . “e(aNe JOURN ~ () 69>, 0% (peY'ez'0z WOd ae + | ‘siss90 “Wx “joury wen | we Wesel | i Be XOdL over van | suyk eersera | accisza sessoia aieseia oH (B1)'Lt'9b'(oh) ssersca cor | ouraso oe ozsszo zze'ez zee'ez HeStZa even) | were, C | ecnsea Txaz0 | poze} paz | ewz0 | ~ sno | sborzose | -ror-zose | -rorzose | -so1-z0s2 2002 ‘Zt Key ove 107 Wedey WISpUEL WOUS Jo SSMeUY TOM] FEL Lesizvage #889 Koustiy tol-20sz *# 0889 3008 bariaeza)