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M. Sc. Biochemistry
(Semester I-IV)
Choice Based Credit System (CBCS)
Department of Biochemistry
Saurashtra University
Rajkot
Course
CBC 1
CBC 2
CBC 3
IBC 1
PBC 1
CBC 4
CBC 5
CBC 6
IBC 2
PBC 2
CBC 7
CBC 8
EBC
EBC 1
EBC 2
PBC 3
CBC 9
CBC 10
EBC
EBC 3
EBC 4
PBC 4
Name of Paper
SEMESTER 1
Fundamentals of Biochemistry and Microbiology
Metabolism I
Protein Structure, Function and Enzymology
Biostatistics and Bioinformatics
(Interdisciplinary)*
practical
Assignment
SEMESTER 2
Cell Biology and Membrane Biochemistry
Metabolism II and Nutritional Biochemistry
Basic Concepts of Molecular Biology
Analytical techniques in Biology
(Multidisciplinary / Interdisciplinary)*
Practical
Seminar
SEMESTER 3
Human Physiology and Endocrinology
Advanced Molecular Biology
Elective Course (Any one)
Microbial Biochemistry*
Pharmaceutical Biochemistry*
Practical
Dissertation I**
SEMESTER 4
Clinical Biochemistry
Immunology
Elective Course (Any one)
Plant Biochemistry *
Biochemical and Environmental Toxicology *
Practical
Dissertation II**
Hours / Week
Credits
04
04
04
04
04
04
04
04
12
02
06
02
04
04
04
04
04
04
04
04
12
02
06
02
04
04
04
04
04
04
12
12
06
04
04
04
04
04
04
04
10
12
06
08
Total Credits
96
SEMESTER 1
2
CBC 2: METABOLISM- I
Measures of Central tendency: Mean, Mode and Median, Frequency Distribution, Measures of
Dispersion: Range, Variance, Standard deviation, Coefficient of variation, Confidence limits and
Confidence intervals. Confidence Limits and Confidence Intervals. Chi-square Test
UNIT 2: Statistical Tests in Biology
Students t Test, Meaning of Significance and Significance Levels. Paired and Unpairedt Test.
Analysis of Variance. Regression and Correlation Analysis
UNIT 3: Bioinformatics and Biological Databases
Current Perspective and Emergence of Bioinformatics and its Applications. Primary and
Secondary Database. Database Searching and Database Management. Alignments and Matrices
UNIT 4: Gene Prediction, Genome analysis and protein structure Prediction
Methods of Gene Prediction in Microbial Genomics and in Eukaryotes. Comparative Genomics
and Functional Genomics. Alignment of Protein Structures and its Prediction. Structural
Modelling
SEMESTER II
CBC - 4: CELL BIOLOGY AND MEMBRANE BIOCHEMISTRY
UNIT 1: Structure and Function of Cells and Cell Organelles
6
Evolution of Cell, Introduction to Cell, Cell Types including Cellular Specialization and
Differentiation, Differences in Plant and Animal Cells, Eukaryotic Cell Organelles Structure,
Functions and Biochemistry, Structure and Biochemical Aspects of Specialized Plant Cell
Organelles, Cell Plate, Primary and Secondary Cell Walls, Plasmodesmeta, Importance of
Vacuoles
UNIT 2: Membrane Biochemistry
Types and Sub Cellular Location, Chemical Composition of Biomembranes, Model of Lipid
Membranes, Differences between Biomembranes and Artificial Phospholipids Membranes,
Physical and Biochemical Methods to study Membrane structure and Properties, Membrane
Asymmetry, Lipid Protein Distribution and Interactions, Membrane Lipid Mobility: Transverse
and Flip-flop Movement, Functions of Biomembranes: Energy Transduction, Signal Recognition,
Specialized forms of Membranes-brush Border.
UNIT 3: Tissue Organization and Cytoskeleton:
Cell Differentiation, Organogenesis, Morphological, Functional and Biochemical Maturation of
Tissues, Cytoskeleton: Microtubules Polymerization, Dynamic, and Functions, Microtubules in
Cell Division, Role of Cytoskeleton Filaments in Cancer
UNIT 4: Cell Cycle
Phases of Cell Cycle, Functional Importance of each Phase, Molecular Events during Cell Cycle,
Check Points, Cyclins and Protein kinases, MPF (maturation promoting factor), Regulation of
Cell Cycle. Apoptotic Pathway and Cell Death.
Structure and Properties of Nucleic acids. Secondary and Tertiary structure of DNA, A, B and Z
Types of DNA, Major and Minor grooves. Structure and Properties of RNA, Cot Value and
Satellite DNA. Prokaryotic and Eukaryotic Gene Structure and Organization.
UNIT 2: DNA Replication
Importance of DNA Replication, DNA Polymerases, Other Enzymes and Protein involved in
DNA Replication, Mechanisms and Regulations of DNA Replication, Inhibiters of DNA
Replications, Major Differences between Prokaryotic and Eukaryotic DNA Replication.
UNIT 3: Transcription
Transcription in Prokaryotes, RNA Polymerases, Promoters, Initiation, Elongation and
Termination of RNA synthesis, Inhibitors of RNA Synthesis, Concept of Reverse Transcriptase.
Post-transcriptional Modifications.
UNIT 4: Translation
Basic Features of Genetic Code, Mechanisms of Translation, Ribosome Structure, A and P sites,
Importance of Different Codon, Regulation of Gene Expression in Prokaryotes: Enzyme
Induction and Repression, Operon Concepts, LAC Operon, TRP Operon
Theories of Tissue Fixation and Staining Techniques. Principles of Transmission and Scanning
Electron Microscopy. Principles of Phase Contrast and Fluorescence Microscopy. Principle and
Applications of Autoradiography
UNIT 2: Spectroscopy
Basic Principles of Spectroscopy, UV, IR, Raman, ESR, ORD. CD and Structure of Proteins
using NMR and ESR. Neutron and X-Ray Diffraction for Elucidation of 3D Structure. Molecular
Modelling, Mass Spectrometry
UNIT 3: Chromatographic Techniques
Basic Principle and types of Chromatography. Gas Chromatography, GC-MS, LC MS / MS.
Ion Exchange Chromatography, Gel permeation, Affinity and Reverse Phase Chromatography.
HPLC and FPLC
UNIT 4: Centrifugation and Electrophoretic Techniques
Principle and Applications of Centrifugation Techniques. Basic Principles of Electrophoresis,
Agarose Gel, Native and SDS-PAGE. Isoelectric Focusing, 2D-PAGE and their uses in Protein
Research. Fractionation and Blotting Techniques
SEMESTER III
CBC 7: HUMAN PHYSIOLOGY AND ENDOCRINOLOGY
10
11
Purification and Analysis of Nucleic Acids, Characteristics of Bacterial Strains used for Genetic
Manipulations, Properties and Applications of Plasmids, Phagemids, Phage Vectors, Cosmids,
YAC, BAC, etc, Oligonucleotide Synthesis and their uses.
UNIT 2: Gene Cloning and Expression
Construction of Genomic and cDNA Libraries, Molecular Genetic and Reverse Genetic
Strategies. Analysis of Cloned Genes Identification of Promoters and Enhancers,
Determination of Transcriptional Start Site, Intron-exon Organization, Gene Confirmation by
Gene Disruption
UNIT 3: Recombinant DNA technology
Enzymes used in Recombinant DNA Technology Principles and Applications of DNA
Polymerases, Restriction Endonucleases, DNA Modifying Enzymes. Principles and uses of
Nucleic acid Hybridizations, Principles and Applications of Polymerase Chain Reaction,
Principles and Methods of Nucleic acid Sequencing, Immuno-chemical Techniques.
UNIT 4: Protein engineering
Site directed mutagenesis, PCR-mediated Mutations, Applications of RAPDs and DNA Finger
Printing. Cell Cycle, Regulation, Cell Death, Cancer. Role of Bioinformatics in Protein
Engineering.
13
SEMESTER IV
14
and
Infectious
Diseases.
Liver:
Jaundice
and
Cirrhosis.
Kidney:
Properties and Overview of Immune Response, Innate Immunity, Cells and Tissues of The
Adaptive Immune System, Cytokines, Effect or Mechanism of Cell Mediated Immunity, Effect
or Mechanism of Humeral Immunity
UNIT 2: Recognition of Antigens and Maturation, Activation, Regulation of Lymphocytes
Antibodies and Antigens, The Major Histocompatibility Complex, Antigen Processing and
Presentation to T Lymphocyte, Antigen Receptors and Accessory Molecules of T and B
Lymphocytes, Lymphocyte Development and the Rearrangement and Expression of Antigen
Receptor Genes, Activation of T Lymphocyte, B Lymphocyte and Antibody Production,
Immunological Tolerance
UNIT 3: Diagnostic Immunology, Hybridoma Technology and Vaccination
Antigen
and
Antibody
Reactions:
Precipitation
and
Agglutination
Reactions,
Cell Plate, Primary and Secondary Cell Walls, Plasmodesmata, Importance of Vacuoles,
Characteristics of Meristematic Cells.
UNIT 2: Concepts of Photosynthesis and Phytohormons
Photochemistry, Energy Considerations, Light Reaction with Z Scheme, CO 2 fixation, Calvin
Cycle, C3, C4 and CAM, Photorespiration, Chemistry and Action of Phytohormones and Plant
Growth Regulators.
UNIT 3: Secondary Metabolites
Special Features of Secondary Plant Metabolism Formation and Functions of Alkaloids, Phenolic
Compounds, Tannins, Lignins, Flavonoid Pigments, Surface waxes, Cutin and Suberin the
Plant Protective Waxes, Terpenes. Different Types of Bioreactors for Mass Production
UNIT-4: Water Relations of Plants
Role of Water, Absorption, Conduction and Transpiration, Guttation, Water balance and Stress
Physiology. Osmoprotectant
Particulate Matter, Compounds of Carbon, Sulphur, Nitrogen and their Interactions, Methods of
their Estimation and their effect on Atmosphere. Types of Water Bodies and their General
Characteristics, Major Pollutants in Domestic, Agricultural and Industrial Wastes, Methods of
their Estimation, Effects of Pollutants on Plants and Animals. Pollution of Water by Micro
Organisms, Bacteriological Analysis of Water-sewage Treatment for Home and Industrial.
UNIT 2: Effects of Soil Salinity on Plants
Glycophyte Halophyte Plant Defense Mechanism, Effect on Growth, Role of Osmoprotectant,
Effect on Macro- and micro- Nutrient Accumulation
UNIT 3: Chemical Toxicology
Toxic Chemicals in the Environment, Impact of Toxic Chemicals on Enzymes, Biochemical
Effects of Ar, Pb, Cd. Hg, Biochemical Effects of CO, NOx, SOx, Pesticides
UNIT 4: Bioremediation
Bioremediation for Environmental Cleanup. Biomass Utilization and Microbial Degradation of
Xenobiotics.
Reference Books:
18
19
22. Harper's Illustrated Biochemistry by Murray, R. K., Granner, D. K., Mayes, P. A. &
Rodwell, V. W.
23. Human Nutrition and Dietetics by Davidson & Passmore
24. Human Physiology by Devis
25. Instant Notes in Biochemistry by Hames, B. D. & Hooper, N. M.
26. Introduction to molecular biology by Paolella, P.
27. Introduction to Protein Architecture: The structural biology of proteins by Lesk, A. M.
28. Introductory Biostatistics by Chap T. Le
29. Lehninger Principles of Biochemistry by Nelson, D. L. & Cox, M. M.
30. Modern Experimental Biochemistry by Boyer, R.
31. Molecular Biology of The Cell - Bruce Alberts
32. Nucleic Acids by Bloomfield, V. A., Crothers, D. M. & Tinoco, I.
33. Nutritional Biochemistry by Tom Brody
34. Plant Biochemistry by Heldt, H-W.
35. Plant Physiology By Taiz and Zeiger
36. Practical Protein Chromatography by Hutchens, T. W.
37. Principles of Biochemistry by Zubay, J.
38. Protein Biochemistry and Proteomics: Experimental series by Rehm, H
39. Protein purification: principles and practice by Scopes, R. K.
40. Protein structure: a practical approach by Creighton, T. E.
41. Proteins: structure and function by Whitford, D.
42. Textbook of Medical Physiology by Arthur C. Guyton 11th Ed.
43. Water and the cell by Pollack, G. H., Cameron, I. L. & Wheatley, D. N.
20