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Easy DNA Extraction from Anything Living

DNA Genome Project

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DNA Test DNA Extraction
Simple DNA Extraction

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How To Extract DNA

10 Interesting DNA

DNA versus RNA

Extraction Chemistry

Water Extraction

10 RNA Facts

How To Make a DNA
Model out of Candy

By Anne Marie Helmenstine, Ph.D.
Updated July 19, 2015.

DNA or deoxyribonucleic acid is the
molecule that codes genetic information in
most living organisms. Some bacteria use
RNA for their genetic code, but any other
living organism will work as a DNA source
for this project.

DNA Extraction Materials
While you can use any DNA source, some
work especially well. Peas, such as dried
split green peas, are an excellent choice.
Spinach leaves, strawberries, chicken liver,
and bananas are other options. Don't use
DNA from living people or pets, as a simple
matter of ethics.
When you extract DNA, it doesn't look like this!
DNA is visible to the naked eye as a gelatinous
blob. High magnification is required to see the
molecular structure. Richard New stead, Getty Images

100 ml (1/2 cup) of a DNA source

1 ml (⅛ teaspoon) table salt, NaCl
200 ml (1 cup) cold water

enzymes to denature protein (e.g., meat
tenderizer, fresh pineapple juice, or contact lens cleaning solution)
30 ml (2 tablespoons) liquid dishwashing detergent

70-90% rubbing alcohol or other isopropyl or ethyl alcohol

cup or bowl
test tubes

straws or wooden skewers

Perform the DNA Extraction
1. Blend together 100 ml of DNA source, 1 ml of salt, and 200 ml of cold water.
This takes about 15 seconds on high setting. You are aiming for a
homogeneous soupy mixture. The blender breaks apart the cells, releasing the

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If you examine the interface between the alcohol and each sample. CONTINUE READING BELOW OUR VIDEO Test Your General Science Knowledge 0:00 / 3:21 1. 3.Photos an… ' ] _a^kkgÛ " __kqj p4ecj 6 l . sources high in DNA will yield more product at the end. This is the DNA! 5.DNA that is stored inside. but you still need to get it out of solution. How It Works The first step is to choose a source that contains a lot of DNA. 4. You can examine the DNA using a microscope or magnifying glass or place it in a small container of alcohol to save it. The human genome is diploid. Careers and Education Chemistry Facts and Pictures . The enzymes are used to cut the DNA. Why would you want to cut it? The DNA is folded and wrapped around proteins. so it will float on the liquid. Tilt each tube and pour alcohol down the side of each glass or plastic to form a floating layer on top of the liquid. so it needs to be freed before it can be isolated. strawberries are octoploid and contain 8 copies of each chromosome. The other molecules in the sample will dissolve in alcohol. you should see a white stringy mass. Allow this solution to react for 5-10 minutes before proceeding to the next step. the DNA is separated from other cell constituents. meaning it contains two copies of each DNA molecule. Your goal is to remove the large solid particles. Swirl the contents gently to incorporate the enzyme. but DNA does not. Although you can use DNA from anywhere. Harsh stirring will break the DNA and make it harder to see in the container. After you have completed these steps. Pour the liquid through a strainer into another container. Add 30 ml liquid detergent to the liquid. but you don't want to pour it into the tubes because then it will mix. When you pour alcohol (the colder the better) onto the solution. Blending the specimen breaks apart the cells so you can separate the DNA from other molecules. This is where the alcohol comes into play. The detergent also separates the lipids (fats) from the sample. Salt and detergent act to strip away proteins normally bound to DNA. discard the solids. For example. Alcohol is less dense than water. Use a wooden skewer or a straw to capture and collect the DNA from each tube. 2. Keep the liquid. Many plants contain multiple copies of their genetic material. Stir or swirl the liquid to mix it. Add a small pinch of meat tenderizer or a squirt of pineapple juice or contact lens cleaner solution to each vial or tube.

There are lots of facts about DNA. Useful. + • How to Extract DNA From a Banana • DNA Definition and Structure • Quick Facts about Nucleic Acids • 10 Interesting DNA Facts I 25. DNA or deoxyribonucleic acid codes for your genetic makeup. @ 520 7. or fun. Fun.. and Interesting Facts about DNA $al 76 DNA and RNA By Anne Marie Helmenstine.D.com About Education Chemistry . P . important. 1.. Even though it codes for all the information that makes up an organism. the . <a7. 67/1k " J About. * 9 Movies That Horrifyingly Killed People In Real Life The Real Reason Hollywood Won't Cast Katherine Heigl Anymore Pow ered By ZergNet Jackie O's Granddaughter Is Basically Her Twin Hidden Secrets No One Noticed Ever Noticed in These Disney Films ļ Е &17. 5. DNA is built using only four building blocks. Learn More About DNA • • • 10 Interesting DNA Facts DNA Versus RNA DNA Facts Quiz O. . but here are 10 that are particularly interesting.the DNA molecule precipitates so that you can collect it. Ph.

This could include errors during transcription. This means you carry mutations! Some of the mutations cause no harm. About a thousand times a day. although scientists did not understand DNA was the genetic material in cells until 1943. eight hours a day. In other words. If you put all the DNA molecules in your body end to end. A parent and child share 99.nucleotides adenine. 7. a few are helpful. it would take approximately 50 years to type the human genome. You have 98% of your DNA in common with a chimpanzee. but some damage isn't repaired. guanine. you have more in common. 2. . such as cancer. 3. 4. Humans and cabbage share about 40-50% common DNA. 5. CONTINUE READING BELOW OUR VIDEO What Are The Seven Wonders Of The World 0:00 1. and cytosine. while others can cause diseases. damage from ultraviolet light. The deoxyribonucleic acid or DNA molecule forms a double helix. something happens to it to cause errors. There are many repair mechanisms. genetically speaking. the DNA would reach from the Earth to the Sun and back over 600 times (100 trillion times six feet divided by 92 million miles). Scientists at Cambridge University believe humans have DNA in common with the mud worm and that it is the closest invertebrate genetic relative to us.5% of the same DNA. Prior to that time. or any of a host of other activities. 3. thymine. Getty Images 2. with a mud worm than you do with a spider or octopus or cockroach. Friedrich Miescher discovered DNA in 1869. it was widely believe that proteins stored genetic information. If you could type 60 words per minute. Every human being shares 99% of their DNA with every other human. 6. David Fairfield. DNA is a fragile molecule.

* Monica Lewinsky Looks Completely Different Now About Education Pow ered By ZergNet Famous Movie Couples Who Hated Working Together Why Hollywood Won't Cast Sarah Michelle Gellar Anymore Inappropriate Scenes Cut From Popular Kids' Movies P . @ 520 7. +. 672@ * 287#+8l a7/21 /1 . </9. + • Build Your Own DNA Models • What Is DNA? • 10 Useful RNA Facts • DNA Definition and Structure I 25. 5SE. . P . Also from About. 285/1* 2: S Follow us: Enter your email SIGN UP © 2016 About. — All rights reserved. <a7. 77. The Balance & Lifewire Our Story Write for About Site Map Terms of Use & Policies Advertise With Us Help Careers at About .DNA vs RNA O. Inc.com: Verywell. * . .

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