%2C L2 #: 75al 7" J


About Education




Easy DNA Extraction from Anything Living

DNA Genome Project

Chemistry com

DNA Test DNA Extraction
Simple DNA Extraction

( 4qepι a bnki ( kkcha ή$hkq`

How To Extract DNA

10 Interesting DNA

DNA versus RNA

Extraction Chemistry

Water Extraction

10 RNA Facts

How To Make a DNA
Model out of Candy

By Anne Marie Helmenstine, Ph.D.
Updated July 19, 2015.

DNA or deoxyribonucleic acid is the
molecule that codes genetic information in
most living organisms. Some bacteria use
RNA for their genetic code, but any other
living organism will work as a DNA source
for this project.

DNA Extraction Materials
While you can use any DNA source, some
work especially well. Peas, such as dried
split green peas, are an excellent choice.
Spinach leaves, strawberries, chicken liver,
and bananas are other options. Don't use
DNA from living people or pets, as a simple
matter of ethics.
When you extract DNA, it doesn't look like this!
DNA is visible to the naked eye as a gelatinous
blob. High magnification is required to see the
molecular structure. Richard New stead, Getty Images

100 ml (1/2 cup) of a DNA source

1 ml (⅛ teaspoon) table salt, NaCl
200 ml (1 cup) cold water

enzymes to denature protein (e.g., meat
tenderizer, fresh pineapple juice, or contact lens cleaning solution)
30 ml (2 tablespoons) liquid dishwashing detergent

70-90% rubbing alcohol or other isopropyl or ethyl alcohol

cup or bowl
test tubes

straws or wooden skewers

Perform the DNA Extraction
1. Blend together 100 ml of DNA source, 1 ml of salt, and 200 ml of cold water.
This takes about 15 seconds on high setting. You are aiming for a
homogeneous soupy mixture. The blender breaks apart the cells, releasing the

) /k 1 ? 3 @
25 ' 8 5
$5, , J , C 6<, 77, 56

✔ About Today

✔ Living Healthy
✔ Chemistry

Enter your email


! %#I &) L OR
Chemistry 101 - Introduction to Chemistry
Chemistry Tests and Quizzes

Chemistry Demonstrations, Chemistry Exp…
Periodic Table and the Elements

Chemistry Disciplines - Chemical Enginee…

Chemistry Homework Help - Online Study …

Chemistry of Molecules and Compounds -…
Grow Crystals - Crystal Growing Projects …
Science Fair Projects
Chemistry for Kids

Chemistry in Everyday Life - Articles, Hom…
Lab Safety and Toxic Chemicals - Safety i…
Chemistry Laboratory

The detergent also separates the lipids (fats) from the sample. How It Works The first step is to choose a source that contains a lot of DNA. Many plants contain multiple copies of their genetic material. Harsh stirring will break the DNA and make it harder to see in the container. Add a small pinch of meat tenderizer or a squirt of pineapple juice or contact lens cleaner solution to each vial or tube. 2. CONTINUE READING BELOW OUR VIDEO Test Your General Science Knowledge 0:00 / 3:21 1. Pour the liquid through a strainer into another container. 3. Your goal is to remove the large solid particles. you should see a white stringy mass. sources high in DNA will yield more product at the end. Allow this solution to react for 5-10 minutes before proceeding to the next step. Use a wooden skewer or a straw to capture and collect the DNA from each tube. Alcohol is less dense than water. Tilt each tube and pour alcohol down the side of each glass or plastic to form a floating layer on top of the liquid. so it needs to be freed before it can be isolated. This is where the alcohol comes into play.Photos an… ' ] _a^kkgÛ " __kqj p4ecj 6 l . discard the solids. meaning it contains two copies of each DNA molecule. so it will float on the liquid. Add 30 ml liquid detergent to the liquid. Swirl the contents gently to incorporate the enzyme. If you examine the interface between the alcohol and each sample. Careers and Education Chemistry Facts and Pictures . Why would you want to cut it? The DNA is folded and wrapped around proteins. The enzymes are used to cut the DNA. Although you can use DNA from anywhere. After you have completed these steps. When you pour alcohol (the colder the better) onto the solution. Stir or swirl the liquid to mix it. the DNA is separated from other cell constituents. 4. but you don't want to pour it into the tubes because then it will mix. but you still need to get it out of solution. strawberries are octoploid and contain 8 copies of each chromosome. You can examine the DNA using a microscope or magnifying glass or place it in a small container of alcohol to save it. but DNA does not. This is the DNA! 5. For example. The human genome is diploid. Salt and detergent act to strip away proteins normally bound to DNA. Keep the liquid.DNA that is stored inside. Blending the specimen breaks apart the cells so you can separate the DNA from other molecules. The other molecules in the sample will dissolve in alcohol.

Even though it codes for all the information that makes up an organism. the .. and Interesting Facts about DNA $al 76 DNA and RNA By Anne Marie Helmenstine.D. . + • How to Extract DNA From a Banana • DNA Definition and Structure • Quick Facts about Nucleic Acids • 10 Interesting DNA Facts I 25. Useful. 1. important. @ 520 7. <a7. but here are 10 that are particularly interesting. or fun. There are lots of facts about DNA. * 9 Movies That Horrifyingly Killed People In Real Life The Real Reason Hollywood Won't Cast Katherine Heigl Anymore Pow ered By ZergNet Jackie O's Granddaughter Is Basically Her Twin Hidden Secrets No One Noticed Ever Noticed in These Disney Films ļ Е &17. 67/1k " J About.. P . Fun.com About Education Chemistry . Ph.the DNA molecule precipitates so that you can collect it. DNA is built using only four building blocks. 5. Learn More About DNA • • • 10 Interesting DNA Facts DNA Versus RNA DNA Facts Quiz O. DNA or deoxyribonucleic acid codes for your genetic makeup.

such as cancer. a few are helpful. something happens to it to cause errors. The deoxyribonucleic acid or DNA molecule forms a double helix. 4. About a thousand times a day.5% of the same DNA. it would take approximately 50 years to type the human genome. eight hours a day. 3. 5. and cytosine. damage from ultraviolet light. guanine. David Fairfield. the DNA would reach from the Earth to the Sun and back over 600 times (100 trillion times six feet divided by 92 million miles). you have more in common. If you put all the DNA molecules in your body end to end. This could include errors during transcription. DNA is a fragile molecule. thymine. 7. Humans and cabbage share about 40-50% common DNA. There are many repair mechanisms. A parent and child share 99. 2. This means you carry mutations! Some of the mutations cause no harm.nucleotides adenine. 3. In other words. Friedrich Miescher discovered DNA in 1869. it was widely believe that proteins stored genetic information. Getty Images 2. with a mud worm than you do with a spider or octopus or cockroach. although scientists did not understand DNA was the genetic material in cells until 1943. Prior to that time. genetically speaking. while others can cause diseases. Every human being shares 99% of their DNA with every other human. but some damage isn't repaired. If you could type 60 words per minute. CONTINUE READING BELOW OUR VIDEO What Are The Seven Wonders Of The World 0:00 1. Scientists at Cambridge University believe humans have DNA in common with the mud worm and that it is the closest invertebrate genetic relative to us. 6. or any of a host of other activities. You have 98% of your DNA in common with a chimpanzee. .

5SE. . Inc. @ 520 7. . * . Also from About.com: Verywell. + • Build Your Own DNA Models • What Is DNA? • 10 Useful RNA Facts • DNA Definition and Structure I 25.DNA vs RNA O. The Balance & Lifewire Our Story Write for About Site Map Terms of Use & Policies Advertise With Us Help Careers at About . * Monica Lewinsky Looks Completely Different Now About Education Pow ered By ZergNet Famous Movie Couples Who Hated Working Together Why Hollywood Won't Cast Sarah Michelle Gellar Anymore Inappropriate Scenes Cut From Popular Kids' Movies P . +. — All rights reserved. </9. 285/1* 2: S Follow us: Enter your email SIGN UP © 2016 About. 77. 672@ * 287#+8l a7/21 /1 . P . <a7.