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Easy DNA Extraction from Anything Living
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DNA Genome Project

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Human
DNA Test DNA Extraction
Simple DNA Extraction

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How To Extract DNA

10 Interesting DNA
Facts

DNA versus RNA

Extraction Chemistry

Water Extraction

10 RNA Facts

How To Make a DNA
Model out of Candy

By Anne Marie Helmenstine, Ph.D.
Updated July 19, 2015.

DNA or deoxyribonucleic acid is the
molecule that codes genetic information in
most living organisms. Some bacteria use
RNA for their genetic code, but any other
living organism will work as a DNA source
for this project.

DNA Extraction Materials
While you can use any DNA source, some
work especially well. Peas, such as dried
split green peas, are an excellent choice.
Spinach leaves, strawberries, chicken liver,
and bananas are other options. Don't use
DNA from living people or pets, as a simple
matter of ethics.
When you extract DNA, it doesn't look like this!
DNA is visible to the naked eye as a gelatinous
blob. High magnification is required to see the
molecular structure. Richard New stead, Getty Images










100 ml (1/2 cup) of a DNA source

1 ml (⅛ teaspoon) table salt, NaCl
200 ml (1 cup) cold water

enzymes to denature protein (e.g., meat
tenderizer, fresh pineapple juice, or contact lens cleaning solution)
30 ml (2 tablespoons) liquid dishwashing detergent

70-90% rubbing alcohol or other isopropyl or ethyl alcohol
blender
strainer

cup or bowl
test tubes

straws or wooden skewers

Perform the DNA Extraction
1. Blend together 100 ml of DNA source, 1 ml of salt, and 200 ml of cold water.
This takes about 15 seconds on high setting. You are aiming for a
homogeneous soupy mixture. The blender breaks apart the cells, releasing the

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The human genome is diploid. Why would you want to cut it? The DNA is folded and wrapped around proteins. but you still need to get it out of solution. Add a small pinch of meat tenderizer or a squirt of pineapple juice or contact lens cleaner solution to each vial or tube. Keep the liquid. Add 30 ml liquid detergent to the liquid. so it will float on the liquid. meaning it contains two copies of each DNA molecule. Careers and Education Chemistry Facts and Pictures . How It Works The first step is to choose a source that contains a lot of DNA. discard the solids. so it needs to be freed before it can be isolated. but DNA does not. but you don't want to pour it into the tubes because then it will mix. Harsh stirring will break the DNA and make it harder to see in the container. Although you can use DNA from anywhere.Photos an… ' ] _a^kkgÛ " __kqj p4ecj 6 l . Blending the specimen breaks apart the cells so you can separate the DNA from other molecules. 3. Many plants contain multiple copies of their genetic material. When you pour alcohol (the colder the better) onto the solution.DNA that is stored inside. Swirl the contents gently to incorporate the enzyme. You can examine the DNA using a microscope or magnifying glass or place it in a small container of alcohol to save it. Tilt each tube and pour alcohol down the side of each glass or plastic to form a floating layer on top of the liquid. Allow this solution to react for 5-10 minutes before proceeding to the next step. sources high in DNA will yield more product at the end. This is the DNA! 5. For example. the DNA is separated from other cell constituents. After you have completed these steps. Salt and detergent act to strip away proteins normally bound to DNA. The detergent also separates the lipids (fats) from the sample. Use a wooden skewer or a straw to capture and collect the DNA from each tube. 2. Pour the liquid through a strainer into another container. Stir or swirl the liquid to mix it. The other molecules in the sample will dissolve in alcohol. Alcohol is less dense than water. strawberries are octoploid and contain 8 copies of each chromosome. 4. Your goal is to remove the large solid particles. The enzymes are used to cut the DNA. If you examine the interface between the alcohol and each sample. CONTINUE READING BELOW OUR VIDEO Test Your General Science Knowledge 0:00 / 3:21 1. you should see a white stringy mass. This is where the alcohol comes into play.

Ph. Even though it codes for all the information that makes up an organism. * 9 Movies That Horrifyingly Killed People In Real Life The Real Reason Hollywood Won't Cast Katherine Heigl Anymore Pow ered By ZergNet Jackie O's Granddaughter Is Basically Her Twin Hidden Secrets No One Noticed Ever Noticed in These Disney Films ļ Е &17. @ 520 7. and Interesting Facts about DNA $al 76 DNA and RNA By Anne Marie Helmenstine. but here are 10 that are particularly interesting.com About Education Chemistry . DNA is built using only four building blocks. the . 5. DNA or deoxyribonucleic acid codes for your genetic makeup. Useful. Fun. P ..D. or fun. + • How to Extract DNA From a Banana • DNA Definition and Structure • Quick Facts about Nucleic Acids • 10 Interesting DNA Facts I 25. Learn More About DNA • • • 10 Interesting DNA Facts DNA Versus RNA DNA Facts Quiz O.. . There are lots of facts about DNA. important. 1. 67/1k " J About.the DNA molecule precipitates so that you can collect it. <a7.

Humans and cabbage share about 40-50% common DNA. The deoxyribonucleic acid or DNA molecule forms a double helix. In other words. If you put all the DNA molecules in your body end to end. 4. while others can cause diseases. 2.5% of the same DNA. you have more in common. a few are helpful. Friedrich Miescher discovered DNA in 1869. the DNA would reach from the Earth to the Sun and back over 600 times (100 trillion times six feet divided by 92 million miles). 3. About a thousand times a day. eight hours a day. it was widely believe that proteins stored genetic information. thymine. genetically speaking. Every human being shares 99% of their DNA with every other human. You have 98% of your DNA in common with a chimpanzee. If you could type 60 words per minute. 5. it would take approximately 50 years to type the human genome.nucleotides adenine. . DNA is a fragile molecule. 7. but some damage isn't repaired. although scientists did not understand DNA was the genetic material in cells until 1943. such as cancer. 6. damage from ultraviolet light. 3. A parent and child share 99. something happens to it to cause errors. or any of a host of other activities. and cytosine. David Fairfield. This means you carry mutations! Some of the mutations cause no harm. This could include errors during transcription. Getty Images 2. CONTINUE READING BELOW OUR VIDEO What Are The Seven Wonders Of The World 0:00 1. There are many repair mechanisms. Scientists at Cambridge University believe humans have DNA in common with the mud worm and that it is the closest invertebrate genetic relative to us. with a mud worm than you do with a spider or octopus or cockroach. guanine. Prior to that time.

— All rights reserved. 672@ * 287#+8l a7/21 /1 . 285/1* 2: S Follow us: Enter your email SIGN UP © 2016 About. @ 520 7. 77.com: Verywell. The Balance & Lifewire Our Story Write for About Site Map Terms of Use & Policies Advertise With Us Help Careers at About . <a7. * Monica Lewinsky Looks Completely Different Now About Education Pow ered By ZergNet Famous Movie Couples Who Hated Working Together Why Hollywood Won't Cast Sarah Michelle Gellar Anymore Inappropriate Scenes Cut From Popular Kids' Movies P . P . Also from About. 5SE. </9. . * . . +.DNA vs RNA O. Inc. + • Build Your Own DNA Models • What Is DNA? • 10 Useful RNA Facts • DNA Definition and Structure I 25.

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