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MKJ5122
SECTION012
2016/02/28
BIOL 220W
Moyan Jia
mkj5122
BIOL 220W
Moyan Jia
mkj5122
BIOL 220W
Moyan Jia
mkj5122
Sample
DNA ladder
Moyan Jias sample 1 mkj5122
Moyan Jias sample 2 mkj5122
XinTangs sample 1 xzt5037
XinTangs sample 2 xzt5037
Negative control
DNA ladder
Table 2: Wells and Samples Match
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BIOL 220W
Moyan Jia
mkj5122
The DNA result was then analyzed and compared with rCRS via MEGA.
3.0 Results
The gel electrophoresis result demonstrates that all the DNA segments and
negative control are on the same horizontal plate (shown in figure 1), which
indicates the result is not precious. Most experimenters data shows that gels
added master mix displaying non-sense results. The DNA purification was
operated again.
2 3 4 5 6
Figure 1: Gel Electrophoresis under UV, all segments ran same distance
After the DNA sequence was analyzed into specific nucleotides, experimenter
mkj5122s DNA was compared and contrasted with rCRS. MEGA displays and
traces DNA in wave graphs (figure 2). All noisy and non-sense DNAs were
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BIOL 220W
Moyan Jia
mkj5122
BIOL 220W
Moyan Jia
mkj5122
# rCRS
Mutation
Known Exist
166
16140
T to C
Yes[6]
209
16183
A to C
Yes[7]
215
16189
T to C
Yes[8]
220
16194
A to C
Yes[9]
222
16196
Inserted T
No
223
16197
C to T
Yes
227
16201
C to A
No
231
16205
C to A
No
240
16214
C to A
Yes
258
16232
C to A
Yes
260
16234
C to A
Yes
262
16236
C to A
No
265
16239
C to A
Yes
269
16243
T to C
Yes
284
16258
A to C
Yes
308
16282
C to A
Yes
319
16293
A to C
Yes
BIOL 220W
Moyan Jia
mkj5122
BIOL 220W
Moyan Jia
mkj5122
BIOL 220W
Moyan Jia
mkj5122
BIOL 220W
Moyan Jia
mkj5122
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BIOL 220W
Moyan Jia
mkj5122
References
[1] Finnil, Saara, Mervi S. Lehtonen, and Kari Majamaa. "Phylogenetic Network for
European MtDNA." 2001. American Journal of Human Genetics. The
American Society of Human Genetics. 68 (6): 1475-1484
[2] Mckinney, Emily A., and Marcos T. Oliveira. "Replicating Animal Mitochondrial
DNA." 2013. Genetics and Molecular Biology Genet. Mol. Biol.36 (3):
308-15.
[3] BIOL 220W: Populations and Communities Laboratory Manual-Spring 2016.
Penn State University, 1 Mar. 2016
[4] Bandelt, Hans-Jrgen, Anita Kloss-Brandsttter, Martin B. Richards, Yong-Gang
Yao, and Ian Logan. "The Case for the Continuing Use of the Revised
Cambridge Reference Sequence (rCRS) and the Standardization of Notation
in Human Mitochondrial DNA Studies." 2013. Journal of Human Genetics J
Hum Genet 59(2): 66-77.
[5] "More about Haplogroups." 23andMe Customer Care. N.p., n.d. Web. 01 Mar.
2016.
[6] Horai, S., Hayasaka, K. Intraspecific nucleotide sequence differences in the
major noncoding region of human mitochondrial DNA Human 1990.
Genetics. 46 (4): 828-842 .
[7] Zhang, J., Zhang, Z. X., Du, P. C., Zhou, W., Wu, S. D., Wang, Q. L., Chen, C.,
Shi, Q., Chen, C., Gao, C., Tian, C., Dong, X. P. Analyses of the
mitochondrial mutations in the Chinese patients with sporadic
Creutzfeldt-Jakob disease 2015. Human Genetics . 23 (1): 86-91 .
[8] Aquadro, C.F., Greenberg, B.D. Human mitochondrial DNA variation and
evolution: analysis of nucleotide sequences from seven individuals" 1983.
Genetics. 103 (2): 287-312 .
[9] Bilal, E., Rabadan, R., Alexe, G., Fuku, N., Ueno, H., Nishigaki, Y., Fujita, Y., Ito,
M., Arai, Y., Hirose, N., Ruckenstein, A., Bhanot, G., Tanaka, M.
Mitochondrial DNA haplogroup D4a is a marker for extreme longevity in
Japan 2008. PLoS One. 3 (6): e2421 .
[10] Haplogroup Markers:
http://mitomap.org/bin/view.pl/MITOMAP/HaplogroupMarkers
[11] Simple Tree Mitomap (Web)
http://www.mitomap.org/pub/MITOMAP/MitomapFigures/simple-tree-mit
omap-2012.pdf
[12] Kong, Qing-Peng, Yong-Gang Yao, Chang Sun, Hans-Jrgen Bandelt,
Chun-Ling Zhu, and Ya-Ping Zhang. "Phylogeny of East Asian
Mitochondrial DNA Lineages Inferred from Complete Sequences." 2003.
The American Journal of Human Genetics. 73 (3): 671-76. Web.
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BIOL 220W
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mkj5122
[13] Kim, Ki Cheol, Han Jun Jin, and Wook Kim. "Mitochondrial Haplogroup B Is
Negatively Associated with Elite Korean Endurance Athlete Status." Genes
Genom Genes & Genomics 34.5 (2012): 569-73.
[14] Kivisild T, Tolk HV, Parik J, Wang Y, Papiha SS, Bandelt HJ and Villems R
The emerging limbs and twigs of the East Asian mtDNA tree 2002. Mol.
Biol. Evol. 19: 1737-1751.
[15] Andrews,R.M., Kubacka,I., Chinnery,P.F., Lightowlers,R.N.,Turnbull,D.M. and
Howell,N. Reanalysis and revision of the Cambridge reference sequence
for human mitochondrial DNA 1999. Nat. Genet. 23 (2), 147
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