Use of Enzymatic Agent Sunphase For Feeding Layers

Wuhan Sunhy Biology Co., Ltd.
Use of enzymatic agent Sunphase for feeding layers

Introduction
Deficit and high price of feedstuffs of animal origin resulted in the increasing use of all-vegetable diet in
which significant quantity of phosphorus is in the form of phytate and its biological availability is low.
Phytate also can form complexes with divalent cation, starch and protein. Such complexes are difficult to
be broken up in the alimentary tract. Phosphorus bound in phytate is released only in its small amount in
the course of digestion, because there are no associated enzymes in the digestive tract.
These negative properties of phytate may be significantly reduced through introduction of microbiologic
phytase into feed, which can increase the availability of phosphorus and improve the uptake of calcium,
microelements, protein and amino acids. And phytase can release bound phosphorus making it possible
to save the non-organic phosphorus.
Objective
To determine efficiency of enzymatic agent Sunphase (produced by Wuhan Sunhy Biology Co., Ltd,
China) used in the feed mixes for layers.
Materials and methods

Trial place
The study was conducted in State Scientific Institution Siberian Scientific-research Poultry Institute of
Russian Agricultural Academy of Agricultural Sciences (Russia).
Experimental animals
Layers of the parent stock of Sibiryak 2 cross (Plymouth Rock white) aged 182-350 days of life were
used. Each layer was given an individual number by the leg ring tagging and was placed in the individual
cell. The experiment was conducted in accordance with the principles and guidelines approved by the
local breeding habits.
Experimental enzyme
The enzymatic agent Sunphase (5000 U/g) is a new generation phytase, which is intended for splitting
the phytate of the feedstuffs. The recommended dosage in the feed of layers is 80 g/t.
Experimental design
Table 1 Experimental design
Group
Details of feeding
Control
Basic diet
Sunphase
Diet deficient in calcium and phosphorus + Sunphase
Address: No.5, Lingjiashan Nanlu, East-lake Developing District, Wuhan City, Hubei, China, 430074
Tel/Fax: (00)86-27-87537736/87514058 E-mail: overseasale@sunhy.cn
Http: //www.sunhy.cn

Table 2 Composition and nutritional value of the feed for layers, %
Ingredient
control
Sunphase
Wheat
43.091
44.268
Barley
20.000
20.000
Rape-seed cake
3.000
3.000
Sunflower cake
7.000
7.000
Chalky flour
6.371
6.343
Full-fat soya
13.669
13.277
Sunflower oil
2.031
1.783
Fish meal
1.500
1.500
Premix
2.000
2.000
Monocalcium phosphate
0.825
0.303
Methionine
0.100
0.100
Lysine
0.051
0.058
Sodium salt
0.362
0.360
0.008
Sunphase
Index
OK*
K**
Matrix value
Metabolizable energy, KJ/kg
11495
11495
Crude protein
15.700
15.700
Calcium
3.000
2.900
0.100
Phosphorus
0.400
0.280
0.120
Lysine
0.720
0.720
Methionine
0.360
0.360
Methionine + cystine
0.620
0.620
Threonine
0.580
0.580
Note: OK*- nutritional value of basic diet

K**- nutritional value of diet deficient in calcium and phosphorus
Statistical analyses
All statistical analyses were carried out using the SPSS 16.0 for Windows. The means within each
treatment and among treatments were subjected to one-way ANOVA and compared using Duncans
multiple range tests. Differences were considered significantly at P<0.05.
Results and discussion

Use Sunphase in the feed of laying birds enhanced the laying rate per average layer by 6.39 (P<0.05) in
comparison with the control group (Table 3). And during the whole period of the study, Sunphase group

showed better result in terms of egg production per average layer (P<0.05) and egg weight (Table 3).
The daily requirement of feedstuffs for each layer amounted to 161 g. Hence, the main factor which
determines the feed consumption is the egg production and weight: the higher them the lower feed
consumption. On account of this layers of Sunphase group showed less feed consumption of 10 eggs
(P<0.05) and 1 kg eggs (P<0.05) in comparison with control group (Table 3).
Table 3 Effect of Sunphase on laying performance of layers
Items
Control
Sunphase
Laying rate, %
70.85b
77.24a
Egg production, pcs
119.02b
129.76a
Egg weight, g
63.9
64.4
Feed consumption of 10 eggs, kg
2.26a
2.08b
Feed consumption of 1 kg eggs, kg
3.56a
3.24b
Note: different lowercase superscript in the same row means significant difference (P<0. 05).
Table 4 Effect of Sunphase on morphological qualities of eggs

Absolute weight, g
Relative weight, %
Yolk
Albumen
Eggshell
Yolk
Albumen
Eggshell
Shell thickness,
mm
Control
17.36
36.25
6.89
28.69
59.92
11.40
0.378
Sunphase
16.77
37.39
6.74
27.53
61.39
11.07
0.369
Control
18.81
36.80
6.89
30.10
58.88
11.02
0.362
Sunphase
19.18
37.51
7.01
30.11
58.88
11.01
0.356
Control
20.10
38.74
7.27
30.40
58.61
10.99
0.367
Sunphase
19.43
39.39
7.08
29.49
59.77
10.74
0.372
Control
22.01
37.75
7.34
32.80
56.26
10.93
0.347b
Sunphase
23.02
38.72
7.36
33.31
56.04
10.65
0.371a
Control
21.76
40.44
7.20
31.35
58.27
10.37
0.329b
Sunphase
22.46
42.14
7.20
31.27
58.69
10.03
0.346a
Group
238 days
266 days
294 days
322 days
350 days
Note: different lowercase superscript in the same line of the same days means significant difference (P<0. 05).
The absolute egg weights of control group and Sunphase group for the period of 238-350 days
increased by 8.9 g and 10.9 g respectively. But it was found that the increase values of yolk and albumen

were practically equal: in control group-4.40 and 4.19 g, in Sunphase group-5.69 and 4.75 g. When
study the changes of the relative weight of eggs for groups, similar phenomenon was observed in two
groups: increase of the yolk weight, decrease of the weight of albumen and eggshell (Table 4).
With the increase of age, thickness reduction of the eggshell was observed. However, layers of Sunhase
group significantly surpassed the control group in terms of thickness by 0.017 mm (P<0.05) at the end of
the study. Thus, use Sunphase contributed to enhancement of the quality of eggshell, particularly in the
second half of the productive period (Table 4).
Table 5 Effect of Sunphase on digestibility coefficients of nutritive substances, %
Index
Control
Sunphase
Crude protein
83.13b
87.35a
Crude fiber
29.24b
34.78a
Crude fat
77.84b
84.98a
Note: different lowercase superscript in the same row means significant difference (P<0. 05). The following table is the
same.
Digestibility test was conducted at the age of 300 days. Digestibility of crude protein, fiber and fat of
Sunphase group was increased by 4.22, 7.14 and 5.54 (P<0.05) compared with the control group. As it
was already noted that layers of Sunphase group had better laying performance, the correlation
coefficients of laying performance and digestibility of nutritive substances were found as equal (Table 5).
Table 6 Biochemical analyses of liver and bone of layers
Index
Control
Sunphase
Vitamin A, g/g
395.97
394.13
Vitamin E, g/g
47.58
47.87
11.1
9.55
Ash, %
53.88
55.06
Calcium, %
22.80
24.20
Phosphorus, %
9.50
10.83
Liver
Lipids, %
Tibia bones
Use Sunphase did not exert negative influence on depositing of the vitamins in the liver. Content of
vitamins A, E and lipids in the liver of Sunphase group is at the level of control group (Table 6).
The state of mineral metabolism was evaluated with regard to the content of calcium and phosphorus in
tibia bones. More ash, calcium, phosphorous were found in tibia bones when Sunphase was used.

Conclusion
Use of the enzymatic agent Sunphase in the feed mixes deficient in calcium and phosphorus for layers
can improve laying performance, morphological qualities of eggs, digestibility coefficients of nutritive
substances and mineral metabolism in tibia bones.

Use of Enzymatic Agent Sunphase For Feeding Layers

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Wuhan Sunhy Biology Co., Ltd.