INSTALLATION

Table of contents
General environment . . . . . . . . . . . . . . . . . . . . . . . 2
Celly 70 components . . . . . . . . . . . . . . . . . . . . . . . 3
Fluidics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Instrument preparation . . . . . . . . . . . . . . . . . . . . . . 5
Access to fluidics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Control of fluidics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Reagents and electric connections . . . . . . . . . . . . . . 8

Diluent (white connector). . . . . .
Lysing agent (green connector) .
Rinsing solution (blue connector)
Drain (red connector) . . . . . . . .

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.10
.10
.10
.11

<F8> SETUP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
<F1> Reference values . . .
<F2> Date and time . . . . .
<F3> Result Printout. . . . .
<F4> RS232 settings . . . .
<F5> Reagents and waste .
<F4> Other Set Up . . . . . .
Distributor SETUP . . . . . . .

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.13
.16
.16
.18
.19
.20
.23

Prime . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Starting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

1 General environment
Celly 70 should be installed on a clean and stable
benchtop, free of exposition to direct sunlight,
draft (draught) or dust. Celly 70 works at ambient
Temperature (18 to 26C). Reagents should be
stored between 8C 6[46F], and 30C [86F] and
used from 18 [64F] to 26[79F]; note that White
Blood Cell Differential is compromised above 26C
[79F] (electronic setting being adjusted according
to ambient temperature : 20~22C [68~72F] at
Attention

the factory). Reagent temperature is important for

good operation (do not store reagents in a cold
area). Keep Celly 70 apart from all intense sources
of electrical interferences like centrifuges,
motors,...
Keep around Celly 70 at least 20 cm free to insure
proper ventilation. Also take care to not obstruct air
circulation below instrument.

Celly 70 must be operated only by qualified and trained operators. Take seriously blood samples handling. To avoid any accidental exposition to pathogen agents as HIV or hepatisis virus,
it is recommended to wear blouse, gloves and even safety mask and eyeglasses.

Warning

Never put any vial filled with liquid on the top of the instrument

Warning

To move instrument, place hands under chassis (not under front cover). To avoid backache,
maintain back straight and it is highly recommended to move instrument with two people.

Important

Hematology analysers are very sensitive to the quality of power supply. It is mandatory to connect Celly 70 on a grounded plug and to UPS (Uninterrupted Power Supply) if stability of main
voltage is not satisfactory

2 Celly 70 components
2.1 Fluidics

Turret
Dilution Cuvette assembly
Liquid syringes
Vacuum/Pressure syringe
Counting Chambers

WBC Metering Tube

Internal Vacuum/Pressure Reservoir
Waste Pump
Waste Detector

2.2 Hardware
486 mother board
Specific hematology electronic boards
6"1/4 color LCD TFT display

2 serial ports RS232 to SIL and optionnal thermic printer

Parallel port for optional printer

2.3 Power supply

115 or 230 V (autocommutation), 200VA, 50 or
60 Hz
Attention

Fuses : 2 x 2A for 230 V, 2 x 4A for 115 V (fast)

If commutation from 115 to 230 V is automatic, control fuse value before this operation

3 Unpacking
This picture shows how is installed Celly 70 in its
housing without external body. The first step is to
remove the top

4 Instrument preparation
Remove protection films (right door, screen and
turret). For access to turret, remove front cover :
Pull the front cover
straight and progressive-

4.1 Access to fluidics

To have access to
fluidics, open the
right plexiglass
door, then remove
the shield that
propects
chambers : it must
be
removed
straight, the three

holding pins together

4.2 Control of fluidics

After
all
connections,
before
switch
ing
ON,

open black door located on the right side and remove all the clips from electovalves. Keep them in a
safe place, they can be usefull if instrument is
stopped for long time. Check if no tube is bent and
if all of them are correctly fit in valves. For help, refer to celly 70 fluidic schematics, page 73

4.3 Package
It is recommended to conserve original packaging
in case of transportation of instrument.

5 Reagents1 and electric connections

20 cm
(8")
30 centimeters of
space
can be saved by450using
5 liters
mm (17.7")

450 mm(17.7")

420 mm (16.5")

80

0m

(3
2"
)

Container shown to
compare encombrance.
In such case, consumption of HEMAREF II considerably increases

Keep space below instruWeight 25 kg

Installation with reagents on

the floor. Recommended.
For rinse solution, 5 liters containers as shown. Two
advantages :

FIGURE 1.

- different size of containers -> decrease

risk of error
- lower consumption,

Installation Celly 70 (front)

All connectors are located at the back of the

1. For reagents reference codes, refer to reagents appendix

analyzer :

Power cable
Keyboard cable
Printer cable (optional) parallel or serial
RS232 Communication Cable (2, optional for
LIS or DPU printer)

All tubes, color coded, are located at the back of the

analyzer. Reagents containers can be installed on
the level benchtop or on the floor (recommended,
refer to diagram). All Hycel reagents are compatible with all Celly 70 parts.

DB 9 male (COM 2 : serial printer DPU or LIS)

DB 9 female (VGA CRT screen ; not used)
Not used
DB 25 (parallel printer)
DB 9 male (COM 1: LIS or serial printer DPU)
Keyboard

Diluent (white)

Fuse (2A for 230V, 4A for 115 V)

Rinse (blue)
Power switch
Lyse (green)

Power chord

Air syringe purge(yellow)

Waste (red)
Distance between back of
Celly 70 and wall must be
20 cm (8") or more

nt
Dilue

Rinse
Waste

FIGURE 2.

Installation Celly 70 (back)

5.1 Diluent (white connector)

Tygon Tubing: 2 x 4mm diameters (3/32" x 5/32"),
1.5m (4.9 feet) length
HEMATON PLUS : azide-free isotonic solution for
diluting whole blood specimen with Celly

70 analyzer. HEMATON PLUS must be used in order to produce a white blood cell differential analysis.

5.2 Lysing agent (green connector)

Tygon Tubing : 1.5 x 3mm diameters (1/16" x 1/8"),
0.50m (1.8 feet) length.
CELLYSE : it rapidly breaks down red blood cell
membrane, freeing hemoglobin and reducing the
size of cellular debris to a level that does not interfere with white blood cell analysis. In addition, it
contains a cyanide radical that combines with the
free hemoglobin to form stable cyanmethemoglo Warning

bin. Its absorbance is directly proportional to the

hemoglobin concentration over the clinically useful
range.
CELLYSE must be used in order to produce a
white blood cell differential analysis. This reagent
is stable for 30 days at room temperature after
opening.

Agitate bottle to remove water drops condensated on wall (especially in case of high temperatures)

5.3 Rinsing solution (blue connector)

Tygon Tubing: 2 x 4mm diameters (3/32" x 5/32"),
1.5m (4.9 feet) length.
HEMAREF II is highly concentrated enzyme
based, azide-free, isotonic cleaning detergent
solution. It is used in the metering tube during the

counting cycle and provides rinse solution in the

aperture at the completion of the cycle and for shutdown procedure. This rinsing solution is compatible with the plastic parts of the counter.

5.4 Drain (red connector)

Tygon Tubing: 2 x 4mm diameters (3/32" x 5/32"),
1.5m (4.9 feet) length.
Connect red marked tubing to fitting located on the
back of Celly 70. Screw the tap located at the other
side on the top of waste container.

Residues in the waste must be treated according to

local legislation. (Voir Cyanide waste elimination,
page A - 1)

6 <F8> SETUP
Switch on the ON/OFF button at the back of the
analyzer, on the down left side.

Switch the printer on, if any. After program loading, a first menu showing containers levels is displayed.
Press [ESC] key for access to MAIN MENU and
<F8> for access to settings.

Ver
Before doing anything, it is necessary to customize
instrument (laboratory header, printer and external connection, reference values etc.)

To have access to SETUP, press <F8> in MAIN

MENU or move cursor with arrows keys to select
(highlight) SETUP menu and valid with <Enter>
key

6.1 <F1> Reference values

Reference values are already set. But it may be necessary to adapt to local conditions. Also, each laboratory should establish its own reference table.

Ver
Tip

Only one reference table is available. If necessary, it is possible to use veterinary mode to create different human species (male, female, baby etc.)
Results out of these ranges are :
- flagged with an arrow (high) or (low) in run
menu

- red in the results file (DATALOG)

- printed in bold and underlined.

Ver
- Move the cursor with arrows keys or with
<ENTER> key.
Notes

- Edit new value and validate with <ENTER>

- Leave Edition with <ESC>

It is highly recommended to print references table

Value are displayed in International 1 system units

<F1> let the access (with service password) to fine

settings :
Minimum
number of cells
for WBC differential is
set at 1K/L. Under
this value, there is no
differential (not
enough cells for good
accuracy)

MCHC is between
33 and 34
and a value >
38 is impossible :
system alert
Parameters
for interferences between
small RBC and
big Plts
G

(D2)

WBC

50

100

(D4)

(D1)

(D3)

Ver

200

300

400

When there is a valley between low

and high threshold for mids cells,
the threshold is positionned at
the lowest point of the valley.
This adjustment can be modified a posteriori, manually

6.2 <F2> Date and time

Date and Time edition.

Format DD/MM or MM/DD depends of

setting (see date format, page 19)

Ver

6.3 <F3> Result Printout

Printout are in black and white. For color printouts the transfer of results with histograms to host

computer is necessary. In such case, management

of printout is done by LIS

Ver
Epson LX and LQ type, Canon BJ 200, 210, 240,
250, 300 (Epson compatible)
Epson Stylus C80 or compatible
Hewlett Packard printers emulating PCL3, with
parallel port (HP 5650, 5652)
Serial thermic printer DPU 414, 40 columns
11" (28 cm) or 12" (30.5 cm) paper size (except
for thermic printer)
Data are automatically1 printed after each run
or not.

Reference ranges are printed or not

With / without biologic alerts printout
Half width / full width (except with DPU)
histograms are not printed, system and biologic
alerts are only printed with their abbreviation
(1 line per type of alert), after results
With or without header : with header a space is
reserved for headed paper (except with DPU)
Histograms can be printed filled (not available
with the DPU printer) or empty.2

1. It is not recommended to select automatic printout AND

automatic transfer in the same time (interest ?). This is
possible but as these proccesses are not fast, there is not
enough time to transfer data to 2 peripherials during dilution
cycle (30 sec.). If transfer is too long, it will be interrupted to
not delay next count.

2. When histograms are printed filled (in black), they are also
displayed filled (in colors). Note that this option empties ink
cartridges very fast and increases cost per test with no real
interest.

 It is possible to select one printout (with histograms) or two (without histograms) per page
(except with DPU).
<F1> key (not displayed) : raw datas (for research use). Thereafter raw datas are printed
on only one page. It is necessary to wait before

6.4 <F4> RS232 settings

Counts can be tranfered to host during count or

Ver
(and) from datalog.

running a new numeration ; so do not use this

option with automatic printout. This option is
not available with the thermic printer
<F1> SETUP is displayed if a serial printer is selected
Select highest speed possible with RTS/CTS

Access to RS232 settings with laboratory

password (labo by default)
SETUP :

Low transmission speeds are

not
recommended, especially
when
histograms are
transfered.
Time to enter
identity during
count is deVer

6.5 <F5> Reagents and waste

With laboratory password (labo by default)

For CELLYSE, 1 liter vials should used only for

instruments performing more than 150
tests per day, else it
may perempted before
its end.

HEMAREF II

- For HEMATON PLUS and HEMAREF II 1:

4 numeric characters maximum, no decimal,
space or comma
Maximum volume 20 liters
- For CELLYSE :
4 numeric characters maximum, no decimal,
space or comma
Maximum volume 1000 mL
- For Waste
1. Correction factor is more important for HEMAREF II because
its flow depends of position of container, of pump, of age of
pump

 4 numeric characters maximum, no decimal,

space or comma
Maximum volume 50 liters1

6.6 <F4> Other Set Up

Access reserved by password : laboratory password
is <labo> by default. Other passwords and settings
are reserved for service.

Ver

Corrections factors will be determined with the

observation of containers.
1. Remember that bigger is the waste, more it is difficult to
manage (heavy to move, decontamination)

Ver
Language selection : French, English, Spanish,
Italian1 selected with F10
Customizable laboratory name : two lines of 40
characters each
Default mode selection: HUMAN or VET selected with F10
System units selection :selected with F102
International 1

k / mm3

M / mm3

g / dL

SI 2

Giga / L

Tera / L

g/L

1. or other languages provided by local distributor

Date format
DD/MM/YYYY
MM/DD/YYYY
2 characters for day and month, 4 for year
(from 1980 to 2099).
When format is not correct, an alarm window is
displayed reminding the right format. After
pressing <ENTER> key, previous date is displayed in an alarm window, with cursor at the
beginning of field.
Delay before autorefilling : delay before refilling counting chambers from 10 to 60 minutes
2. it is recommended to select units once and for all to prevent
wrong results in various unit systems

 Note

it is better to use 10 minutes (it is only a refilling cycle to prevent apertures and glass pipes to
dry)
Number of count sequences : select with key
<F10> one or two sequences. By default one sequence run to have the highest throughput (> 70
tests/hour). Two sequences counts are more

Note

For Start-Up and calibration procedures, always two counting sequences are performed independantly from this setting
Plt bias (Customizable, from 0 to 15) : this value
is substracted from platelets counts. Use preferably a low value (5 to 10)
Predilution correction factor : 4 numeric characters from 0.75 to 1.25. If 3 decimals are entered, number is rounded with 2 decimals
RDW correction factor : 4 numeric characters
from 0.75 to 1.25. If 3 decimals are entered,
number is rounded with 2 decimals

Note

precise, especially for platelets and allow better

detection of partial clogs.
1 sequence -> speed
2 sequences -> quality

PDW correction factor : 4 numeric characters

from 0.75 to 1.25. If 3 decimals are entered,
number is rounded with 2 decimals
Laboratory password : maximum 22 numeric characters. Small and capital letters are
differenciated. By default <labo>

At the end of installation, it is recommended to print all setups (printer, serial, passwords etc.)
and to keep printouts in safe place (with computer setup for example)

6.7 Distributor SETUP

In <OTHER SETUP> menu, pressing <F1> gives
acces to distributor settings (with distributor
password) :

Limits of cell counts trigerring decontamifactory calibration factors. When instrument is installed,
all laboratory calibration factors are set at 1.00
WBC time of flight reference has been determined in factory
and should not be changed. If atmospheric pressure is affects TOF, modify altitude correction factor (service )
Logos may be changed (service)

Distributor password is distri by default. As this

password can be changed and lost, there is a universal service password :

(<day of week> + <day of month> + <month>) x

123
Exemple : thursday 16th december 2004 ->
(4 + 16 + 12) x 123 = 32 x 123 = 3936

7 Prime
From MAIN MENU select prime or press <F6>
(priming). For the first time, it is recommended to

HEMAREF II

prime reagents one by one. With <F1>, prime

HEMATON PLUS. Control if liquid is correctly
aspirated from diluent container. With <F2> prime
CELLYSE. Control if lyse is well aspirated from lyse
bottle. With <F3>, prime HEMAREF II. Control if

liquid is well aspirated. For security, prime again

reagents all together with <F4>
If there is problem of aspiration, check if all the
tubes are properly fit in valves, if no tube is bent, if
all fittings are waterproof (properly tighten,
enough not too much)

8 Starting
Press [ESC] key for access to MAIN MENU.

HEMAREF II

Select "Startup" menu by pressing <F1> twice

Check that the results obtained are correct (WBC <

0.5, RBC < 0.3, PLT < 30 and 179<Hb channel<255) else validation is denied.

Note

If validation is possible but zeros too high, it is recommended to rerun startup with F10, else
accuracy of results may be affected (residues are substracted from measured values)

TIP

If startup validation is difficult, leave startup procedure and proceed to count with <F3>, with
choice no startup cycle. Perform one or two tests with any blood (even one day old), then
with no tube in the turret (blank or diluent test). Most of time background noise is decreased
to about zero. Last step is to proceed to startup that should be OK
Calibration should be checked with blood controls
before running samples (Voir <F5> Quality,
page 37).

Go to RUN menu (<F3>), insert a homogenized

blood tube into the turret slot and rotate manually
counter clockwise the turret full stop into the instrument. Once the specimen tube is in position

under the sampling needle, the turret is locked in

and the dilution process starts. As the blood is sampled, red light is ON. The light remains red during
the cycle. As sampling is done, the turret rotates automatically back to the initial position and the tube
can be immediately removed. Other operations are

automatic. Put another specimen tube in the turret

slot, when the front green LED light is ON again,
Celly 70 is ready for next sample test by turning turret.
If any error occurs for count, mechanical or fluidic, a flag or message is displayed on the screen.

MAINTENANCE
Table of contents
Access to parts . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Cover removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
Access to chambers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29

Weekly maintenance . . . . . . . . . . . . . . . . . . . . . . . 30
Preventive maintenance . . . . . . . . . . . . . . . . . . . . . 31
Aperture cleaning . . . . . . . .
Turret cleaning . . . . . . . . . .
Needle cleaning . . . . . . . . .
Pump check up and cleaning
Keyboard skin. . . . . . . . . . .
Long time stop . . . . . . . . . .
Turret Kit replacement. . . . .
Hygiene . . . . . . . . . . . . . . .

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.31
.34
.37
.38
.38
.38
.39
.43

9 Access to parts
9.1 Cover removal

Pull cover straight

9.2 Access to chambers

Shield must be removed straight

When it is reinstalled, be careful with
tubes and stirring motor connections

10 Weekly maintenance
- Purge of air syringe : with pressure variations,
condensation of water may occur inside air
syringe, decreasing its effective volume (poor
vacuum, with TOF increasing, incomplete liquid
transfer from dilution cuvettes to counting
chambers).
Open yellow fitting located on the back of the
instrument (see Figure 3, Installation Celly 70
(back), page 11) and let liquid drain by gravity.
A better drain can be done by pulling air syringe
up and down (from main menu, <F7/F1> : Ser Very

important

Rinse tube must be connected to the front of needle as shown on the picture

vices/service, then <air syringe> or during backflush (<F4> clean aperture and open purge fitting)
- Cleaning of bench below Celly 70 : air orifices are
located under instrument and some dust could
be aspirated, spoiling vacuum tubes and syringe.
- Cleaning of turret (See turret cleaning, page 34)
- Control of needle : needle must be straight and
clean and properly installed in its holder

11 Preventive maintenance
11.1 Aperture cleaning
- Before calibration and in case of repeted clog
alerts.
- Always perform this operation in service menu
(in order to prevent any automatic refilling cycle)
- Prepare tissue paper to wipe any drop of liquid

- In service menu (See aperture : drain before dismounting., page 47) drain counting chambers.
- Open the right door and remove counting chambers protection shield (page 8).

Disconnect electrode plug

Disconnect tubes from chambers (do
not pull tubes but push them with nails)

- Follow schematics to dismount apertures (same

procedure for RBC and WBC)
For WBC, bent
pins must be installed in UP and
DOWN positions
as shown

Chamber with aperture holder

FIGURE 3.

Remove the two

bent pins

Pull aperture holder to the

right

Counting chambers aperture dismounting

- There is one oring per aperture to avoid leakages. Remove it carefully to avoid contact with
bleach. Make sure that spare orings are always
avialble in stock

-Prepare pure
bleach
(36
Chl). Put the
apertures
in
bleach solution
at least 15 minutes. Dont let
orings
and
electric connectors in contact
with
bleach,
they would be
Bleach solution
damaged. Two
vials are provided with opened covers in accessory kit.

- Then rinse apertures with deionised water. Dry

with lint free tissue, then reinstall the oring.
- Reconnect tubes
- Reinstall external aperture holders on counting
chambers. Be careful with oring. Push bent pins
in place . Replug electrode. Re-install the cover
shield, right side first : be careful ! do not pinch
tube(s).

- Leave service menu by pressing <ESC>.

- Run several counts on diluent to make sure that
background counts are correct.

11.2 Turret cleaning

Recommended every week for 70 numerations/
day.
Remove the front cover (page 28)
Select the test TURRET DISMOUNTING in service menu
Disconnect the three transfer tubings (yellow, orange1 and red)
and spring

1. orange fitting is for RBC/Plt : tube is

specific (rubber tubing, reference
524100055).

Pull the turret up, do not bend needle.

Clean inside and outside of the turret with absorbent paper moistened with cleaning and disinfectant liquid (no solvent such as alcohol, some water
with dishes detergent or HEMAREF II is quite convenient). Clean also black plexiglass (no alcohol nor
solvent)
Caution

Never put fingers in dilution cuvettes ! Else, it is required to clean them

Reinstall turret taking care of electromagnet
plunger position.

Attention

Clean turret axis. If necessary lubricate it slightly

(just a film)
Clean the dilution cuvettes with a cotton tip moistened with HEMAREF II or ID109 (strong detergent).

Reinstall fitting transfer tubings to appropriate

color fitting and spring plug.
After complete installation run 2~3 samples
with bleach in place of sample and then few
counts on diluent

Do not dismount dilution and evacuation chambers, except if absolutly necessary : plactic is
very thin and fragile. If cuvette holder is dismounted, think to put some silicon grease on
screws before re-installation, to avoid oxydation or (an) deposit of salt

11.3 Needle cleaning

Warning

Needle
cleaning with
nylon wire
FIGURE 4.

Cleaning the internal side of the needle

being delicate, it should be done only by
service staff :
- go to service menu to prevent automatic rinsing

- Remove needle from potence and disconnect tube from the top
- use the nylon wire provided in the
accessory kit, push it from top to bottom.

- remove front cover (page 8)

- Reinstall needle, tube(s), front cover,

quit service and run background(s)

needle must be absolutely fully inserted, with rinse tube in the front, otherwise the external flow of diluent cannot rinse the needle properly, and a large
contamination may occur.
Attention

Needle must be clean.

Salt deposit as shown
here is acceptable. Clean
with tepid water

Needle must be absolutely straight ;

during the external rinsing, the liquid
must follow the needle from top to bottom, else needle remains dirty. If necessary, stripe needle with very thin sand

paper (800) from top to bottom (never in

other direction!). Then it must be soaked
to help liquid flow (put ID109 or liquid
soap on tissue paper and apply on needle), followed by blank cycles

11.4 Pump check up and cleaning

Recommended every 6 month.
Cleaning of draining pump :
Remove the front cover
Disconnect red labeled tubing located on the
right of sampling needle
In place of this tubing, connect white labeled silicone of accessory kit (length 0.5m [1.6 foot], external 6mm [1/4"])
Put the end of this tubing in a vial filled with 50
ml (about) of Hemaclean.

Select the test <drain sink> in service menu. The

pump starts for 12s and hemaclean is aspirated
from vial. Repeat operation until vial is empty.
Do not use water else pump will stop after 2 seconds
With 50 mL, vial should be almost empty in 2 sequences
Then disconnect silicone tubing and reconnect
original tubing1.
1. Repeat this cleaning if necessary. Then if aspiration is not
correct, pump must be renewed (complete pump or only
internal membranes, call distributor)

11.5 Keyboard skin

Regularly check keyboard skin. In case of tearing,
it is recommended to replace it.

11.6 Long time stop

It is not recommended to let an hematology instrument idle for a long period. As tubes are all filled
with salty solutions, risks of crystallization are very
high, with as consequences tubes
clogged, sometime impossible to clean (lyse, evacuation of draining chambers).
To let the analyser idle over one week (or for
storage) :

1. Drain reagents by priming on air :

- remove tubings from reagent containers
(HEMATON PLUS, HEMAREF II, CELLYSE)
- from main menu, press <F6>, prime ALL
REAGENTS. Repeat operation twice.
2. Prime with water (deionised)
- place tubings in deionised water and prime
(ALL REAGENTS).

- press <ESC> to return to main menu.

3. Counts on water (deionised)
- press <F3> and perform three counts on water.
- press <ESC> to go back to main menu.
4. Drain reagents by doing a prime on air :
- repeat step 1
- switch off the instrument

5. Reinstall springs on pinch valves1

in case some springs are lost , remove pinched
tube from front valve slot of the double valves (1,
2, 3, 4, 5, 9, 11 and 12) : tube will not be damaged
after long stop
1. Dont forget to remove springs or to reinstall tube before
restarting

11.7 Turret Kit replacement

Reference G710070
This kit includes :
- Turret
- Cuvettes and cuvette holder

- Base
- Optical sensors (installed)
- Solenoid (installed)

11.7 .1 Removal
of assembly

Before all remove the complete fluidic module

To remove turret base, remove

the three screws holding base

and loosen these two screws

Disconnect all plugs (optical sensors, solenoid,

tubes), remove the belt.
To disconnect
optical
sensors
connectors press
with a thin screwdriver there

To reinstall assembly, there is no adjustment. Be

carefull with belt. Re-fit tie-raps on cables to get a
clean installation.

11.8 Hygiene
External covers of the instrument should be regularly dust off (using a wet duster) and decontaminated with a disinfectant moist towel.
Do not forget
the turret
the keyboard skin

the printer buttons

All components of the analytical system should
be decontaminated by the laboratory staff as
explained in the preceding paragraphs before
any service intervention.

TROUBLE SHOOTING
Table of contents
WBC counting time (WBC time of flight [tof]) . . . . . . 46
WBC tof too long. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46
WBC tof too short . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46

Fluidic troubles . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Drain trouble . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
Transfer trouble . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
Checking valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48

Reagent problems . . . . . . . . . . . . . . . . . . . . . . . . . 49
No lysing agent. . . . . . . . . .
No diluent . . . . . . . . . . . . .
No rinsing solution . . . . . . .
Inversion between reagents .

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Other . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
MCV too low . . . . . . . . . . . . . . . . . .
WBC differentiation . . . . . . . . . . . . .
WBC histogram width . . . . . . . . . . .
Hemoglobin high and WBC very high .
High Plt count on diluent . . . . . . . . .
Plt results low . . . . . . . . . . . . . . . . .
Poor repetivity . . . . . . . . . . . . . . . .
Plt results too low . . . . . . . . . . . . . .
RBC and Plt results . . . . . . . . . . . . .

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12 WBC counting time (WBC time of flight [tof])

TOF reference can be modified in Setup (only by
service). To determine TOF, with a machine perfectly clean (apertures), run counts on diluent.
There should be no variation on TOF (maximum

0.1 second). Compare TOF measured to TOF in

setup and change this one if necessary.
Note that TOF is measured and set when automatic calibration is performed.
Reference time should be around 8 seconds.

12.1 WBC tof too long

Dirty aperture:
Protein building on apertures reduces the diameter and flow rate. This is affecting particles
counts and sizing. Such troubles are exceptional
when daily maintenance is properly done.
Clogged apertures :
Fibrin, crystals, micro-clot in blood can block
the apertures. Dirty apertures clog more frequently. If the problem is persistent, check for
reagent cleanliness and proceed to a cleaning
procedure (aperture cleaning, page 31). Also,
check if aperture oring is well fit.

Vacuum too low

Check for silicone tubing air leaks (V6, V8, V9,
V12, V11) control if tube in V5 is well pinched
Detergent HEMAREF II missing
Problem with air syringe : purge it (see access
to parts, page 28)
Atmospheric pressure too low. A correction is
available. This event happens especially in high
altitude location.

12.2 WBC tof too short

Air leakage in aperture, counting fluidics or metering tube.
Salt deposit in the metering tube.
Low solution level in counting chamber, lack of
diluent, incomplete transfer to counting cham-

bers. Check valves 4, 5, 6, 10 and 12 (see transfer

trouble, page 47).
Lack of HEMAREF II. Check if present in metering tube.
Lack of HEMATON PLUS

13 Fluidic troubles
13.1 Drain trouble
Bad drain can be caused by :
valves problems (see checking valves)
pump failure because of salt deposit on membrane (refer to pump check up and cleaning,
page 38 to solve problem)
Draining detector failure :
- Select service menu.

- Disconnect red labeled tubing located on the

right of sampling needle
- Connect a syringe filled with HEMATON PLUS
(at least 10cc) to the fitting connected to the
drain detector.
- Select fluidic detector in service menu and check
drain detector when pushing successively air and
HEMATON PLUS.

13.2 Transfer trouble

Incomplete transfer to the counting chambers :
some liquid can flow out of the front of instrument.
Check if counting chambers are tight : be sure
that orings are correctly fitted (1 per aperture)
Check fluidic connections on turret. Avoid to
pinch the tubings.
Check if there is no clog in outputs of dilution cuvettes.
Check valves 4, 5, 12 and 11 (see reagent problems, page 49).
Note

Look for pinched or clogged tubings ; disconnect the tubing from valve V4 for WBC ; for
RBC, there is no valve between dilution cuvette
and counting chamber. Connect a syringe filled
with distilled water to the tubings : push and
pull water in tubings.
Check vacuum/pressure syringe (see air syringe (vacuum/pressure), page 45) and purge it
(access to parts, page 28)

Instrument OFF for more than a week, check transfer before starting in order to avoid overflows at start-up.

13.3 Checking valves

Do all valves operate properly (see tests, page
46).
Some tubings can be pinched in valves. Remove
them from valves one by one and roll them between fingers, then re-install them. Make sure
that tubings are not twisted.

Check for silicone tubings damages ; replace

them with same size tubing (in accessory kit,
1.98 / 3.18 mm (1/8" / 1/12") for two-way valves
and 1.58 / 3.18 mm (1/8" / 1/16") for oneway
valves).

14 Reagent problems
14.1 No lysing agent
Numeration of WBC very high, bad differentiation,
Hb high. See example hereafter

WBC histogram is
saturated (because
without lyse, RBC
are counted).

Valve problem : check valve 3 activation

Tubing out of reagent in the bottle
No more reagent in bottle : replace it

Pinched or sticked feeding tubing if left idle too

long : check tubings in valve V3. Both tubings
are silicone 1.98 / 3.18 mm (1/8" / 1/12"). They
should not be bent nor sticked.

14.2 No diluent
Lack of precision and even rising drift
Empty container : replace it

Tubing out of the container

Check valves 1 and 2 (activation and pinched
tubes)

14.3 No rinsing solution

Empty container : replace it
Tubing out of the container

Check valves 8, 9 and 12 (activation and

pinched tubes)

14.4 Inversion between reagents

If HEMATON PLUS and HEMAREF II are inverted (or HEMAREF II everywhere) cell membranes
are affected by surfactants present in HEMAREF
II. Consequently :
MCV is very low

WBC differential is abnormal (only one peak

in Lym position)
Hb is normal
Most of blood controls are normal (in blood controls as cells membranes are very strong they
resist more than patient cells)

15 Other
15.1 MCV too low
Check if the oring is well fit in RBC aperture assembly.
An ionic short-circuit may appear between RBC
and WBC : change silicone tubing in valves V7
and V10.

Make sure that veinous blood is sampled with

EDTA K3

15.2 WBC differentiation

WBC differential analysis should preferably be
done on fresh blood sample (more than one 1/2
hour, less than 4 hours) and well mixed (2-5
minutes but not over-mixed) and with an ambient temperature between 18 and 26C (64 to
79F). Also take care of reagent temperature :
in case of storage at a temperature very different from laboratory temperature, let balance
minimum one day.
The anti-coagulant should always be the same
(dilution ratio, anti-coagulant quality,), preferably EDTA K3.
Caution

WBC aperture slightly dirty (see aperture

cleaning, page 31)
Inversion between HEMATON PLUS and HEMAREF II : this drags WBC histogram to left
and only one peak remains in Lympho position.
MCV is decreased
Lysing agent is affected by too high temperatures (>26C) and its efficiency may decrease
after 2 weeks. For laboratories doing few tests
per day, 250 mL bottles are recommended.

with blood control, WBC differentiation may remain correct if HEMATON PLUS and HEMAREF II
are inverted

15.3 WBC histogram width

WBC gain should be adjusted in order to have the
end of granulocytes distribution between 300 and
330 fl for normal human samples. The average of
lymphocytes peak is around 60 fl.

Lysing agent is affected by too high temperatures (>26C) and its efficiency may decrease
after 2 weeks. For laboratories doing few tests
per day, 250 mL bottles are recommended.

15.4 Hemoglobin high and WBC very high

Probably lack of lysing agent : open the right door
(black plexiglass), remove counting chamber shield
and look at the lyse reaction in the WBC chamber
(red, turbid solution should become translucid).
Check presence of lyse in bottle. Replace if necessary
Improper mixing : check motor (page 46)

Sticked or clogged tubing : check tubings in

valve V3
If necessary, replace the two silicone tubings
1.98 / 3.18 (1/8" / 1/12") (thin wall, do not use
tubings with thick wall in double way valve).
In case of both high RBC and high Plts, check
correct inflow of Hematon Plus (no bubbles in
tubings).

15.5 High Plt count on diluent

1. Container
Empty diluent container : replace it
Plunger out of container
2. Pollution :
Pollution of dilution cuvettes : The best way to
get rid of the problem is to rub the inner sides of
the dilution cuvettes with a cotton tip impregnated with Hemaclean or better with pure
ID109. ID109 is a strong cleaning solution for
decontamination you may use either diluted to
prime the Celly, or pure to impregnate a cotton

tip and rub the dilution cuvettes. You may order

it : p/n : 571300170 Designation : ID109 cleaning solution. Do not pour the Hemaclean or
ID109 in the cuvette (NEVER POUR PURE
ID109 INSIDE THE CUVETTES), just rub the
empty cuvette inner sides with the cotton tip impregnated. Then start directly a startup cycle.
No more than 3 consecutive startup cycles
should be required to obtain acceptable results.
Bad rinsing of needle : refer to needle cleaning,
page 37

 Too much grease around the diluent piston and/

or the sample piston : Disassemble the diluent
syringe and the sample syringe from the dilutor
assembly. Wipe with a soft tissue both the pistons and the inside of the syringe body to eliminate all visible grease. A very very thin film of
silicone (provided by Hycel) grease is sufficient
for efficient lubrification (not visible, just
enough to make the o-ring bright). Any excess
will drive to high Plt background counts.
Pollution of the reagents : Replace both HEMATON PLUS by new containers.
Pollution inside the sampling needle : refer to
needle cleaning, page 37.
3. Electronic noise :
Bad earth connection : Check the earth connection from the laboratory mains installation to
the main plug of the instrument. Do not forget to
check the power cord and eventual extension
cords.

Note
Attention

Noisy mains : Use an on line UPS with sinus

output. Be careful as some UPS are sometimes
more electrically noisy than the mains directly
from the wall plug.
Bad shield : Verify that the counting chamber
shield is well inserted. Verify that the pre-amplifier board shield is well screwed. Using an
ohmmeter, check the ground continuity between
the pre-amplifier shield and the pre-amplifier
board ground map, then between the pre-amplifier board ground map and the chassis. Control also spring plug ()
Incorrect electronic adjustments : Verify Plt offset, gain and threshold adjustments. A too high
offset, too high gain or too low threshold might
drive to high background Plt counts.
Some electronic components of pre-amplifier
board are very sensible and can be damaged by
electric chocks. Contact service department
Aperture oring damaged or dirty : a ionic short
-circuit in parallel to aperture generates high
level of noise (change it).

When important noise appears and disappears, usually it is a problem of electric contact
In any case after such adjustment it is mandatory to check with blood (control and patient)
validity of settings

 Attention

It is recommended to let only service people to do these settings

15.6 Plt results low

On human bloods : needle may be dirty, refer to
needle cleaning, page 37

15.7 Poor repetivity

On RBC/Plt (and WBC)
Insert properly needle in its holder
Clean needle inside and outside
Check diluent fluidics

Control mixing procedure

Bent sampling needle
Needle may be too high or tow low (see sampling needle, page 46)

15.8 Plt results too low

Control mixing procedure

Be sure that sampling needle does not touch bottom of tube/vial (see sampling needle, page 46).

15.9 RBC and Plt results

Needle may be bent or position not proper
(height, position to remove drop...) refer to sampling needle, page 46

Control WBC cuve (position, cracked ?)

ANALOGIC ADJUSTMENTS (MEASURE)

Table of content
Measurement principles . . . . . . . . . . . . . . . . . . . . . 56
Problems of centering and recirculation . . . . . . . . . . . . . . . . . 56
What is what? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Synoptic of measure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

Hb adjustments . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
HGB offset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Hb channel setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

Offset adjustment on RBC, WBC channels . . . . . . . . . 62

RBC offset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Platelets offset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
WBC offset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

Analog board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Counting thresholds adjustments . . . . . . . . . . . . . . . . . . . . . 64
Swirling back adjustments . . . . . . . . . . . . . . . . . . . . . . . . . . 64

Analog gains adjustment

. . . . . . . . . . . . . . . . . . . . 67

RBC gain adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

PLT gain adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
WBC gain adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

Preamplifier schematic . . . . . . . . . . . . . . . . . . . . . . 69
Analog board schematic . . . . . . . . . . . . . . . . . . . . . 70

16 Measurement principles
16.1 Problems of centering and recirculation
%
130
PIV
110
PIII
PII

PI

80

PI
60
E

0.5%

PII

E
40

PIII

20

0.5

1.5

x/d

(aa)

5
10
20

95

12

30
40
50
60
70
80

0 2 05 0

When cells are not

well centered in aperture (PII, PIII, PIV),
there is distortion of
pulses.

PIV

11

90

100

96

10
10

1 00

-0.5

5
110
125

RIII
RII

After going through the aperture,

because of turbulences, cells go
back near aperture, giving small
long pulses (RI, RII, RIII) that
must be eliminated from sizing and
counting (anti recirculation). In
Celly 70, RBC reference electrode
is long to aspirate sample close to
aperture and a filter rejects too
long pulses. For adjustment, refer
to swirling back adjustments, page
64

RI

100 %

PI

80
60
(a)

RIII

40
20

RI

RII

Pulses are very long

compared with PI
Fig 5 : Centering and recirculation

Trigger
Level
t

(ab)

16.2 What is what?

-Plt threshold removes noise from measure. Too high threshold
eliminates small platelets.
-RBC thresholds eliminates platelets and noise from RBC
count. In case of very low MCV (goat, sheep...), this
threshold may be decreased (with interferences of
Plt on RBC count) else some RBC are rejected

Plt gain
RBC gain
RBC pulse

Amplitude

Plt pulse

RBC threshold
Plt threshold

Time

Noise

Fig 6 : Aspect of signal

On preamplifier, offset and gain can be adjusted :

Offset : when input is at zero, output is at
zero. The noise (in green on above diagram) is symmetric around the base line.
Adjustment with voltmeter.
Gain : adjusts the amplitude of pulses, so
is responsible of measure of volumes. For

a d j u s tPLT
ment, reference material is
Increase of DC gain
necessary : usually fresh blood control,
with for RBC, value of MCV, for Plt value of
MPV. For WBC peak of Lymp gives a first
approach
RBCbut final adjustment is done on
Increase
of DC gain
patient
bloods.
fL

10

20

30

100

200

300

fL

WBC
Increase of DC gain

16.3 Synoptic of measure

Gain

Hb
Offset
WBC and Hb channels
use the same converter

Elimination of negative pulses

P (Z80)

From P : choice Hb/WBC

ADC
WBC

Pulse OK
Pulse
Elimination of negative pulses
WBC pulses
5V

Anti recirculation

Low threshold
Gain

P (Z80)

Gain

P (Z80)

ADC

ADC
RBC + Plt
RBC

Plt
Pulse OK

Pulse OK
Offset

Offset

Pulse

Pulse

Plt pulses

RBC pulses
5V

Anti recirculation

Low threshold

Fig 7 : Schematics of signal processing (analogic)

5V
Low threshold

Anti recirculation

17 Hb adjustments
17.1 HGB offset
Disconnect the Hb LED connector at the Pre-amplifier Board input (J2).
Connect a special adaptor at the Hb output of the
Pre-amplifier Board (black Cinch cable.).

Connect a voltmeter according to the polarity and

adjust the offset voltage just upper 0.0 mV with the
potentiometer indicated on the Pre-Amplifier
Board cover. Reconnect the Hb LED connector.

17.2 Hb channel setting

It enables the user to adjust the Hb reference
channel if necessary.
Press <F4>. The instrument fills the counting
chambers with diluent if they are empty. (If an au-

tomatic rinsing has been done, the chambers are already full).
Then, the Hb reference value is displayed.

GAIN PLT

OFFSET PLT

OFFSET RBC

GAIN RBC

OFFSET
H
WBC
B

GAIN WBC

OFFSET Hb

RBC WBC
RBC

G
A
I
N

WBC

If the Hb value is out of

range (180 to 254), the instrument beeps continuously every two seconds
and a message is displayed
until the reference is adjusted.
Adjust the hemoglobin
gain potentiometer (on
preamplifier board, near
the metering tube) to
reach 235 - 2451 at 19 21C. This adjustment
must be done according to
the ambient temperature
(the Hb reference decreas-

es when the temperature increases : about 1 channel for 1C).

Once the adjustment is correct, leave the menu
with the <ESC> key.
The new Hb reference value is automatically
memorized and the S8 alert message (if any) is not
set during next counts. ()
Hb calibration is not affected by new Hb reference
channel in the range 180 ~ 254
1. As long as Hb reference channel is in 180~254 range, results
are OK. Nevertheless, higher it is, better is resolution
(precision). Experience in the field shows that between 235
and 245, results are the best and there is enough margin for
small drift. But, in any case, it is highly recommended to
warm-up instrument during about 30 minutes in order to have
good stabilization of power supplies and temperature

Important

This adjustment has to be done only after cleaning WBC chamber. Normally there is no reason
to change adjustment

Warning

Avoid direct strong light interference (sun or light spot) when plexigass smoked door is open.
Install counting chamber shield
When adjustment is over, press <ESC> to quit and
go back to Service menu.

Fig 8 : Preamplifier implantation

GAIN PLT
Pot 10

Pot 9

J5

B200272-ED3
Hycel

OFFSET PLT
PLT
RBC
WBC
HB

J6

5
4
3
2
1

Gnd Gnd
Id
D+
DBUS Gnd

OFFSET H
WBC
G
B

Pot1
Pot2

Pot 4

Pot 5

Pot 8

OFFSET RBC

Pot7

G
A
I
N

GAIN RBC

GAIN WBC

OFFSET Hb

RBC WBC
RBC

J2
J4
LED Hb RBC

J3
WBC

WBC

J1
Hb sensor
Carte pre-ampli
Preamplifier board
B200272

18 Offset adjustment on RBC, WBC channels

For the following adjustment,
the ground must be connected
to the analog controller on the
GND test point. Any other
ground point may result in
wrong adjustment.
Some CINCH connectors are
connected to preamplifier outputs and are accessible on the

Plt
RBC
WBC
Hb

back of instrument. From top to bottom, Plt, RBC,

WBC and Hb. For RBC, WBC and Plt, values are 3
times higher than on corresponding test points
(JP1 for RBC, JP5 for WBC and JP3 for Plt).
A connection kit is available, reference :
534 500 130, including 4 cables.
The adjustment screwdriver is included in starting
kit.

18.1 RBC offset

Connect an oscilloscope on test point JP1 (RBC
INPUT) on the Analog Board and test point GND.
Adjust the offset to 0,00 V with the best precision.
RBC offset potentiometer is located on the pre-amplifier board.

GAIN PLT

OFFSET PLT

OFFSET RBC

GAIN RBC

OFFSET
H
WBC
B

GAIN WBC

OFFSET Hb

RBC WBC
RBC

G
A
I
N

WBC

18.2 Platelets offset

Connect an oscilloscope on test point JP3 (PLT
INPUT) on the Analog Board and test point GND or
connect a Cinch cable equipped at the other end
with an oscilloscope connector on the first connector (from the top) at the back of the analog controller.

GAIN PLT

OFFSET PLT

OFFSET RBC

GAIN RBC

G
A
I
N

Adjust the offset to 0,00

V with the best precision. PLT offset potentiometer is located on the
pre-amplifier board.

OFFSET
H
WBC
B

GAIN WBC

OFFSET Hb

18.3 WBC offset

Connect an oscilloscope on test point JP5 (WBC
INPUT) on the Analog Board and test point GND or
connect a Cinch cable equipped at the other end
with an oscilloscope connector on the third connec Note

RBC WBC

tor (from the top) at the back of the analog controller.

Adjust the offset to 0,00 V with the best precision.
WBC offset potentiometer is located on the pre-amplifier board.

Always check during a diluent count that the offsets are properly adjusted and that the background signals are stable and less than
- for Plt,120 mV peak-to-peak if connected at the back of the analog board
- for RBC, 60 mV peak-to-peak if connected at the back of the analog
- for WBC,50 ~ 60 mV peak-to-peak if connected at the back of the analog board

19 Analog board
Important

For the thresholds and swirling back adjustments, the ground must be connected to the GND
test point on analog controller board. Any other ground point may result in wrong adjustments.

19.1 Counting thresholds adjustments

1. RBC : connect the + of a voltmeter on test point
JP2 and adjust the voltage to 150 mV +/- 0.5 mV
with potentiometer P1.
2. Plt : connect the + of a voltmeter on test point
JP4 and adjust the voltage to 65 mV 0.5 mV
with potentiometer P3.

3. WBC : connect the + of a voltmeter on test point

JP6 and adjust the voltage to 200 mV +/- 0.5mV
with potentiometer P5.

19.2 Swirling back adjustments

Note:

These adjustments must be done with an oscilloscope (1 VDC, 10S). It is advisable to perform
these adjustments during blood counts (e.g. normal control blood) as it is easier to visualize
the signal in such conditions.
1. RBC : connect an oscilloscope on test point JP8
and adjust the pulse length to 50 S with potentiometer P2.
2. Plt : connect an oscilloscope on test point JP9
and adjust the pulse length to 48 S with potentiometer P4.

3. WBC : connect an oscilloscope on test point

JP10 and adjust the pulse length to 50 S with
potentiometer P6.

 Note

See on next page test points on diagram

Fig 9 : Analog board implantation

65 0.5mV with P3

200 0.5mV with P5

Red (RBCinput)

White (WBC input)

WBC threshold

Black (Hb input)

Plt threshold

Yellow (Plt input)

JP13
J5
U19

J4

J3

U23

CA3100

U12

CA3100

U17

JP6
WBC
threshold

+ +

JP4
TL071
Plt
threshold

U2

U11

U13

U5

4066
P5

JP1 RBC input

JP3
Plt
input

P3 P4

+
+

CA310

P1 P2

U8

GND

RBC
antirecirculation

U3

U16

JP7

U20

CA310

AD7575

AD7575

U18

U4

CA310

U1

U10
AD7575

JP5
WBC
input

RBC threshold

J1

P6

WBC antirecirculation

Plt antirecirculation

JP12

+
JP2 RBC threshold

U22

74LS132

U9

U15
4066

74LS132

JP 10
WBC
Bring

50 S with P6

4538

74LS132
JP 8
RBC
Bring

JP 9
Plt
Bring
U14

U24

U21

U15

4066

U6
4538

48 S with P4

4538

150 0.5mV
with P1

50 S with P2

20 Analog gains adjustment

For the gains adjustments, all calibration factors
must be set to 1 (see Calibration menu). A normal
blood control must be used, in control mode. Check
its expiration date and make sure it was kept in
good conditions and properly mixed. Low or high
controls are not recommended.

Offsets, thresholds and anti-recirculation must be

set before these adjustments.
Instrument must be perfectly clean (apertures, reagents, no background noise)
Remember that when gain increases, histogram
drifts to the right.

20.1 RBC gain adjustment

Adjust the RBC gain in order to get the target MCV
indicated for the normal blood on the Control Assay Sheet.

On human sample, RBC indices should be within

normal ranges.

20.2 PLT gain adjustment

Adjust the PLT gain in order to get the target MPV
indicated for the high control blood on the Control
Assay Sheet. The usual mobile PLT high threshold
is close to 20 fL. Sometimes it's difficult to see it. On
human blood MPV is 9 fL. A partial blocking of the
orifice increases the MPV (MCV too, but less).
Sometime the blocking is so low that MPV is the
only parameter affected.
Caution

Run human samples to check the PLT histograms

shape.
A low PLT gain adjustment may result in high PLT
results as, in such case, small RBCs may be counted
as PLTs.
On normal human samples, the average PLT high
threshold is found between 20 and 30 fL.

Normally threshold is set in valley between Plt and RBC distributions. But if big platelets are
proportionally important, some rippling may appear on the right of Plt histogram and threshold
can be set in wrong position. This happens more with low Plt counts and on low controls. In
such case, use manual moving thresholds to correct software interpretation

20.3 WBC gain adjustment

1. With blood control : check that thresholds are in
right positions (choose control mode, not
patient), especially between Lymphos and Mids.
End of Grans population should be between 350
and 360 fL. No alarm should be displayed (D1
means gain too low) and 3 part diff should be

Always re-check offset adjustments after final gain adjustments as they may slightly have
changed.
L

(D2)

50

100

(D4)

(D3)

WBC
(D1)

Caution:

correct
2. With patient blood : when samples are normal
average of end of Grans should be between 310
and 320 fL, maximum 330. Use several blood
samples to have a good idea of adjustment.

200

300

End of Granulos population

on
patient
blood : 320 to 330 fL

400

21 Preamplifier schematic
J2

U3
VI
VO
Com

V+

U13
VI VO
Com

C42

R6

V+

+5V

V+

2
1 Hb LED

POT1 (gain Hb)

U6

C4

C5

D2

V+

R20

V+

V+

J1

C38

V-

+24V

R14

R13

J3

C9
R11

Gnd

R12

V-

C46

+24V

WBC
POT5 offset WBC)
C31

R19

C10

R51

R45

R37

C25

R21

R49

C52

C27

R53
C50

V-

R23

C54
R52

V+

C40

V+

D5

U10

D3

R56

R54

C51

V-

R24

R30
R29

Q4

+5V

R42
C23

TL071

TL071

C35

R33
R31
R25

R43

C19
R26

R7
R9

V+

in
nc
nc
/shdn

R8

LT1121_H

PLT
RBC
POT10 (offet Plt)

U12
RBC

WBC
HB

5
4
3
2
1

Gnd Gnd
Id
D+
DBUS Gnd

J6

TL071
R47
R44

POT8 (offset RBC)

R45
C36

R41

VV-

U8

C24

-V

C33

C18

C16 C7

POT9 ((gain Plts)

+V U11

U9

G
4

R32

J5
1

V-

RBC
J4
/Plt

C32

out
adj
gnd
nc

R35

C34

C21

5 4

3 2

R10

POT7 (gain Plt)

R27

in 8
nc
nc
/shdn

R34

R28
C22

R22

C55

C45

V+

R40

C49
C53

C44
-V

C20

U4

LT1121_H
+24V

R50

CA3100

V+

out
adj
gnd
nc

C17 C8

Molex 6pts

C26

C39
+V

V-

C48 C15

V+

D4
R55

+24

V-

WBC

TL071

C14

R17

R1

Hb

U7

R18

C29

D1
R5

TL071

C1

TL071

POT2 (offset Hb)

U2

R3

TL071

R39

WBC

U5

3
2
1
HB photo
sensor

C43

R4

C28
R15

U1

C30

R16

C12

Q2

POT4 (gain WBC)

R38

C13

R36

V+

V+

Hb

C11

C3

R2

CA3100
C6

R48

C2

C41

RBC

Carte pre-ampli
Preamplifier board
B200272

22 Analog board schematic

LM317 LH
1

CLK Z80A

3
Vin

VCC

Vout

16
5
17
3
1
2

VCC
ADJ

15

AIN
CLK
VREF

14
13
12
11
10
8
7
6

080
081
082
083
084
085
086
087

TP
CS
RO
AGND

A PB0
A PB1
A PB2
A PB3
A PB4
A PB5
A PB6
A PB7

JP12
RBC

Busy
-12V

WBC

VCC

JP1

Hb

VCC

RBC

4 8 1

4538

Jack J1

JP8

6
3
CA3100

14
15

P2
75

100K

12
11
4538

VCC

2
1

+12V

3
+12V

Vin

4
5

JP2

LM78LO5ACZ

1
Vout

AC

CX

VCC

+T
-T
Q

3
13
AST1
4066

10

CA3100

10

CX

Pulses RBC

74LS132

+T
-T

3
P1
10k

AC

75

GND

1
2
3
4
5
6
7
8
9
10

Plt

VCC

VCC

4 8 1

13
8

10

+12V
7416

74LS132
-12V

JP13
CLK Z80B

3
Vin

VCC

Vout

16
5
17
3
1
2

VCC
ADJ

15

AIN
CLK
VREF

14
13
12
11
10
8
7
6

080
081
082
083
084
085
086
087

TP
CS
RO
AGND

B PB0
B PB1
B PB2
B PB3
B PB4
B PB5
B PB6
B PB7

VCC
VCC

Platelets

4 8 1

4538

JP9

6
3
CA3100

14
15

P4
75

100K

12
11
4538

VCC

2
1

+12V

3
+12V

LM78LO5ACZ
Vin

4
5

AC

P3
10k

CX
VCC

+T
-T
Q

C P80
C P81
C P82
C P83
C P84
C P85
C P86
C P87
CLK Z80C
pulse WBC
RST3
START
COM
VCC

13

4066

11

10

8
4

10

TL 071
45

Pulses Plt

10

74LS132

+T
-T
A

GND

3
6

AC

CX

Vout

VCC

B P80
B P81
B P82
B P83
B P84
B P85
B P86
B P87
CLK Z80B
pulse Plt
RST2

Busy
-12V

Jack J3

VCC

A P80
A P81
A P82
A P83
A P84
A P85
A P86
A P87
CLK Z80A
pulse RBC
RST1

VCC

12

+12V
7416

74LS132

+12V

-12V
-12V
1

3
Vin

VCC

CLK Z80B

Vout

3
1
2

VCC

ADJ

16
5
17

15

AIN
CLK
VREF
TP
CS
RO
AGND

Hemoglobin
Jack J5

4066
9

B PB0
B PB1
B PB2
B PB3
B PB4
B PB5
B PB6
B PB7

JP7
VCC

Busy

4066

14
13
12
11
10
8
7
6

080
081
082
083
084
085
086
087

1
VCC

13

6
+12V

+12V

-12V
+

VCC
VCC

WBC
2

Jack J4

VDD

4 8 1

4538

VCC

JP10

6
3
Vin

CA3100

15

75

100K
VCC

LM78LO5ACZ

2
1
4
5

1
Vout
3

GND
+

+
2

12
11
4538

+12V

+12V

P3
10k

AC

6
CA3100
4 8 1

AC

VCC

+T
-T
Q

6
13
4066
74LS132

+T
-T
Q

3
8

5
4

10
VCC

5
74LS132

-12V

V+

Pulses WBC

10

CX

5
4

CX

7 5

2
+

14

P6

+12V
7416

1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50

FLUIDICS INSTALLATION
23 Celly 70 fluidic components
Tube N

tube

length (mm)

material

Tube N

tube

length (mm)

material

1.98 / 3.18

105

Silastic

21

1.98 / 3.18

215

Silastic

1.98 / 3.18

290

Silastic

22

1.98 / 3.18

50

Silastic

1.98 / 3.18

70

Silastic

23

1/16 - 1/8

205

Tygon

1.98 / 3.18

70

Silastic

24

1/16 - 1/8

30

Tygon

1/16 - 1/8

15

Tygon

25

1.57 / 3.18

85

Silastic

1.98 / 3.18

125

Silastic

26

1.57 / 3.18

60

Silastic

1.98 / 3.18

130

Silastic

27

1.57 / 3.18

25

Silastic

1.98 / 3.18

60

Silastic

28

1.98 / 3.18

165

Silastic

1.98 / 3.18

60

Silastic

29

1/16 - 1/8

15

Tygon

10

1.57 / 3.18

50

Silastic

30

1.98 / 3.18

285

Silastic

11

1/16 - 1/8

15

Tygon

31

1.57 / 3.18

110

Silastic

12

1/16 - 1/8

20

Tygon

32

1.98 / 3.18

180

Silastic

13

1/16 - 1/8

80

Tygon

33

1/16 - 1/8

105

Tygon

14

1/16 - 1/8

85

Tygon

34

1/16 - 1/8

55

Tygon

15

1.98 / 3.18

170

Silastic

35

1/16 - 1/8

15

Tygon

16

1.98 / 3.18

235

Silastic

36

1/16 - 1/8

60

Tygon

17

1.98 / 3.18

155

Silastic

37

1/32 - 3/32

25

Tygon

18

1/16 - 1/8

300

Tygon

38

1/16 - 1/8

255

Tygon

19

1/8 - 1/4

500

Tygon

39

1/16 - 1/8

65

Pharmed

Tube N

20

tube

1/16 - 1/8

length (mm)

305

material

Tygon

Tube N

40

tube

1/16 - 1/8

length (mm)

85

material

Tygon

24 Celly 70 fluidic schematics

Sample syringe
Diluent syringe Seringue chantillon Needle rinse
Rinage aiguille
Seringue diluant

Lyse syringe
Seringue lyse
Lyse input
Arrive lyse

Tubes filled with grey are

located in valves front slot

10
9
1

V2

V4

V3

40

5
18

13

15

From WBC cuvette

De cuvette blancs

12

7
14

V1

37

11

V6

Metering tube
tube volumtrique

Les tuyaux remplis en gris

sont positionns dans la fente
avant des vannes

V5

38

RBC chamber
Chambre GR

WBC chamber
Chambre GB

39

From RBC cuvette

De cuvette rouge

17
20

16
29
27

30
21

V8

V9

31
V11

V10

V12

22

26

23
24
33

32

A
IR

34

ge

19

30

28

25
V7

36
32

Detergent input
Arrive detergent
Diluent input
Arrive diluant

A ir

sy

Dr
a
d in d
tec ete
teu cto
r
r li
qu
ide

Pump
pompe

Vacuum / pressure jar

rservoir pression / vide

rin

From sink cuvette

De poubelle

25 Celly 70 fluidic diagram

Diluent
Sample

Single valve closed

Lyse

Double valve
Liquid syringes

Lyse input
2

18

V1

V3
7

V4
15

14

13

15
RBC
count

WBC
count

17

29

RBC

start
Air syringe
stop

16
32

27
28

19

21
V7

V8

V9

V10

V11

30

22

26

red
33

34

16

Waste pump

V12
23

yellow
Air syringe purge

Vacuum / pressure
reservoir

25

orange

28

31

30

Atmosphere

WBC

V6

12

V2
5

11

V5

Sampling
needle

Sink

green

drain
detector

yellow

metering tube

Waste

red

Detergent blue
Diluent

white